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Biomedical subjects

A B Poletaev

Publications and source records attributed to A B Poletaev.

At least 37 records · Page 2Linked to original sources
PubMed1985

[Possible role of brain-specific proteins S100 in the regulation of phosphorylation-dephosphorylation of endogenous substrates of the nerve tissue].

The effects of brain-specific proteins S100 (BSP S100) and their endogenous protein ligands (both cationic and anionic) on the phosphorylation of macromolecular substrates from rat brain and liver were studied. It was shown that BSP S100 significantly affects both the phosphorylation and dephosphorylation of various substrates, depending on BSP S100 endogenous protein ligands.

Animals↗
PubMed1984

[Detection and isolation of glycoproteins and nuclear proteins in the bovine brain].

Using ammonium sulphate precipitation, ion exchange chromatography and preparative isoelectrofocusing, 9 organ-specific glycoproteins and 16 specific nuclear proteins were isolated from bovine brain nervous tissue in a homogeneous state. The isolation of proteins was controlled by a solid phase immunoenzymatic analysis. The molecular weight, subunit composition and isoelectric points of the proteins were determined and their ability to interact with immobilized calf thymus DNA and concanavalin A was demonstrated. It was assumed that the multiplicity of specific proteins of brain tissue is a molecular basis which provides for the functional specificity of the nervous tissue at large.

Animals↗
PubMed1983

[Effect of proteins S100 on RNA and protein biosynthesis in neurons and glial cells of the rat brain].

The effects of brain-specific proteins S100 injected into lateral ventricles of rat brain on biosyntheses of RNA, proteins and endogenous proteins S100 in neurons and glial cells were investigated. It was found that exogenous proteins S100 essentially and irreversibly stimulate RNA and total protein syntheses in glial cells, while bovine serum albumin stimulates RNA and protein syntheses in neurons but not in glial cells. The dynamics of changes in the content of newly synthesized endogenous proteins S100 in glial cells and neurons are suggestive of transport of S100 protein molecules synthesized by glial cells, into the neurons. The absence of appreciable changes in the indicated parameters in rats injected with total rabbit sera proteins as well as in animals subjected to "false injections" provide evidence for the specificity of metabolic effects of proteins S100 and serum albumin.

Animals↗
PubMed1982

[Biospecific preparation and some properties of molecular factors, interacting with brain-specific proteins S100].

The biospecific preparation and a preliminary analysis of physico-chemical properties of water-soluble proteins from bovine brain and liver and brain oligopeptides specifically adsorbed on a column with immobilized brain-specific proteins of the major fraction S100 were carried out. Using thin-layer electrophoresis on cellulose and tachyphoresis, it was found that four oligopeptide cations and three anionic oligopeptides interact with S100. Polyacrylamide gel electrophoresis revealed at least six brain proteins and 11 liver proteins, which interact with S100. An essential role in interaction of protein ligands from the bran (but not from liver) with immobilized S100 proteins belongs to Ca2+. The use of natural endogenous ligands for the study of the biological role of brain-specific proteins S100 is discussed.

Animals↗
PubMed1980

[Heterogeneity of brain-specific proteins of S100 type].

A procedure for preparative separation of proteins from bovine brain precipitated in a saturated solution by ammonium sulfate (pH 4.0) and remaining in the supernatant is described. It was shown that not less than 22 fractions (up to 28 fractions considering the "equivocal" reaction) produce different responses to the antiserum against the major protein, i.e. S100.

Animals↗
PubMed1977

[Immunochemical analysis of glial and neuronal fractions of rat brain].

A method of obtaining antineuronal and antiglial immune sera is described. Data of the quantitative immunochemical analysis of the antigens of the neuronal and glial fractions of the rat brain are presented. The neurons proved to contain 4, and the glia--3 brain-specific proteins; one or two of them are common for these cell fractions.

Animals↗
PubMedDate not supplied

[Antibodies against nerve tissue proteins S100, GFAP, and MP65 and pregnant women with early toxemia].

Serum levels/affinities of natural autoantibodies to proteins S100, GFAP and MP65 in patients with physiological pregnancy are usually constant and may vary among individuals within relatively narrow range. In patients with early toxicosis in pregnancy dispersion of serum autoantibodies levels were often found beyond the normal range. This group includes cases with significantly elevated as well as abnormally decreased immunoreactivity parameters. Percent of newborns with different deviations from women with abnormal serum levels/affinities of autoantibodies to proteins S100, GFAP and MP65 was higher 1,5-2 fold compared with population.

Adolescent↗
PubMedDate not supplied

[Effect of antibiotic therapy on embryotropic antibody level in women].

Embriotropic antibodies (e-Ab) serum contents were analysed in 29 serum samples obtained from women suffering with primary lues. Before treatment the content of e-Ab against MBP, S100, ACBP14/18, and MP65 were markedly elevated in 28 samples. Two injections of Retarpen (2.5 mln Units with one week interval) were enough for effective treatment of lues. At the same time treatment caused deep and reversible inhibition of e-Ab production. One or two months later the levels of e-Ab returned to normal values in 21% of women, in 52% patients the hypoproduction, and in 27% the hyperproduction of e-Ab were noted. Deviations of e-Ab production after effective retarpen treatment suggest the existence of additional reasons which may influence e-Ab production, and necessity of additional diagnostic and curative measures for such women during pregravidal period.

Adult↗
PubMedDate not supplied

[Effect of antibodies to different brain-specific proteins on the formation of motor reactions in the rat].

On Skinner and darkness passive avoidance models, a study was made of the formation of purposive behaviour in rats following an artificial change in the metabolism of brain-specific proteins S100, CP-25 and S60-200 (the two latter isolated for the first time) by means of intraventricular injection of corresponding antibodies. (Administration of non-immune antibodies was used as control.) None of them exerted any influence on motor activity, use of water and food or on speed of learning. Change in the metabolism of CP-25 did not affect elaboration of habits. Action on S100 and S60-200 produced a stage by stage change of habit formation typical of each of them. An assumption was made of connection of S100 with the apparatus of afferent synthesis and of connection of S60-200 with the process of discordance.

Animals↗