We have produced monoclonal antibodies directed against defined amino acid sequences of rhodopsin by using peptides for hybridoma selection, rather than for immunization. Mice were immunized with rhodopsin, and hybridoma culture supernatants were assayed by ELISA to detect anti-rhodopsin antibodies. These antibodies were further tested by competition ELISA using synthetic peptides from the rhodopsin sequence in order to select antibodies with the desired amino acid sequence specificity. Site-specific monoclonal antibodies selected in this way have affinities for the protein which are typically higher than that of antibodies which have been generated against peptide-carrier conjugates as antigens.

Amino Acid Sequence↗

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Synthesis of 14C labelled 1-(N'-carboethoxyhydrazion)-phthalzine hydrochloride ("Binazin").

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Antihypertensive Agents↗

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[Classification of central nervous system sarcomas].

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Brain Neoplasms↗

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Optimization of peptide synthesis on polyethylene rods.

Multipin solid-phase peptide synthesis is widely used for epitope mapping of monoclonal and polyclonal antibodies. However, neither the chemical yield nor the homogeneity of products currently match those of solid-phase synthesis of peptides on resins. In order to improve synthesis parameters, we have repeated the standard procedure and introduced modifications during synthesis of model heptapeptides and peptides from the sequence of rhodopsin and other proteins. Good incorporation of amino acids using the multipin peptide synthesis system can now be obtained in less synthesis time and with less costly reagents.

Acetamides↗