Search PubMed⌕ Search

Biomedical subjects

A Abraham

Publications and source records attributed to A Abraham.

At least 109 records · Page 6Linked to original sources
PubMed1985

Thymic functions in uremic rats: evidence for thymosin alpha 1 deficiency.

Changes in the circulating lymphocyte populations and thymus glands were studied in rats with experimental chronic renal insufficiency (CRI). Compared to normal or sham-operated animals, rats with CRI had significant reduction in the percentages of circulating T and B lymphocytes. CRI was also associated with marked thymic atrophy and reduction in the numbers of small cortical and medullary thymic lymphocytes. Quantitative microfluorometry revealed a significant reduction in the intrathymic concentration of a potent immunomodulator, thymosin alpha 1 in all uremic animals. There was a significant positive correlation between the percentages of circulating T lymphocytes and the intrathymic concentrations of thymosin alpha 1.

Animals↗
PubMed1984

Glomerulocystic kidney disease. Report of an adult case.

A case of glomerulocystic kidney disease (GCK) in an adult is reported. The patient described here had no other congenital malformations, and there was no family history of renal disease. The histopathologic findings support the glomerular origin of GCK. This case illustrated that GCK may be associated with stable renal function for many years. In view of this, GCK should be considered in the differential diagnosis of cystic kidney disease in the adult.

Adult↗
PubMed1984

High-pressure liquid chromatographic separation of glycosylated and acetylated minor hemoglobins in newborn infants and in patients with sickle cell disease.

High-pressure liquid chromatography on a synchropak CM-300 column was used for separation and quantitation of glycosylated and acetylated minor hemoglobins and major hemoglobins in 15 newborn infants, 17 patients with sickle cell anemia and one each with SC condition, CC disease, Hb S heterozygosity, and Hb C heterozygosity. Hemoglobin Bart's, Fla1, Fla2, Flb, Flc, FO, Ald, AO, and A2 have been separated from newborn infants. Similarly, hemoglobins Fla, Flb, Flc, FO, Sla+b, Slc, A2, Sld, and SO were separated from patients with SS condition. Minor hemoglobins in all the samples were also separated by conventional Bio-Rex 70 chromatographic methods. The percentages of the various hemoglobin components determined by high-pressure liquid chromatography agreed rather well with those obtained by Bio-Rex 70 chromatography. However, the quality of the resolutions and the sharpness of the peaks were, in general, superior with the high-pressure liquid chromatography approach, which is much faster, requires significantly less sample than Bio-Rex 70 chromatography, and is particularly suitable for fast analytical chromatography of minor and major hemoglobins.

Acetylation↗
PubMed1984

Rapid purification of avian influenza virus for use in enzyme-linked immunosorbent assay.

A rapid and easy purification method was developed to obtain avian influenza antigen for use in immunochemical assays. This was achieved by rapid concentration of virus from infective allantoic fluid, using 8% (w/v) polyethylene glycol 8000, and later, by purification on gel-permeation chromatography through controlled-pore glass beads. Rabbit anti-turkey globulins were made specific for turkey globulins, using affinity chromatography, conjugated to horseradish peroxidase and used in enzyme-linked immunosorbent assay. A significant increase in specificity and sensitivity of the enzyme-linked immunosorbent assay was observed when purified antigen was used in place of a crude antigen preparation. This purified antigen eliminated the false-positives obtained as a result of the turkeys being previously vaccinated with egg-grown virus vaccines (Newcastle disease virus). The details of the technique and the importance of antigen preparation are discussed.

Animals↗
PubMed1984

Glycosylated hemoglobins in heterozygotes and homozygotes for hemoglobin C with or without diabetes.

Glycosylated hemoglobin in red blood cell hemolysates of five patients homozygous for CC, 18 patients with SC condition, and 13 patients heterozygous for Hb C with or without insulin-dependent diabetes mellitus were separated by Bio-Rex 70 chromatography. The various glycosylated components were identified by analysis of the hemoglobin components for ketoamine and phosphate, in vitro glycosylation studies, and by the quantitative differences in the minor components between the participants with and without diabetes. The percentages of Hb A1a + b, Hb A1c, and Hb C1c were significantly increased in the Hb C heterozygote with diabetes. Similarly, the percentages of Hb S1a + b and Hb S1c were elevated in the SC patient with diabetes. It was noteworthy that the levels of these components became normal after adequate control of diabetes. Moreover, the levels of Hb C1c in the CC participants and Hb S1c (Hb S1c/total Hb S) in the SC patients were significantly higher than the Hb S1c levels previously reported in patients with sickle cell anemia. These findings might reflect the fact that CC and SC patients have less severe hemolytic anemia. Moreover, the relative proportions of Hb A1c and Hb C1c were nearly the same in Hb C heterozygotes, which indicated that Hb A and Hb C were glycosylated in vivo to approximately the same extent.

Anemia, Sickle Cell↗
PubMed1984

Carcinomas of the thyroid gland invading larynx and trachea.

Twenty-nine patients with a thyroid carcinoma invading the larynx and trachea were treated over a 25-yr period. Eight patients had invasion of the walls of the trachea or larynx and 21 had invasion into the lumen. Of the 15 patients who underwent limited surgery, i.e. total thyroidectomy, tracheostomy and neck dissection, 8 had intraluminal invasion and 7 suffered from bleeding into the trachea or airway obstruction. In contrast, none of the 13 who underwent extensive surgery, i.e. thyroidectomy, laryngectomy, partial resection of the trachea and one resection of the pharynx, (12 of whom had intraluminal invasion) showed either bleeding into the trachea or airway obstruction. Although the prognosis was no better in the group undergoing extensive operation there is the feeling that in cases with intraluminal invasion extensive surgery is indicated to prevent the severe airway difficulties which often develop in such patients.

Adult↗
PubMed1983

Demonstration of a minor hemoglobin with modified alpha chains and additional modified hemoglobins in normal and diabetic adults.

We have attempted to separate various hemoglobins in 10 normal and 11 diabetic persons by Bio-Rex 70 chromatography at 4 degrees C with an exponentially increasing sodium phosphate gradient. Minor hemoglobins, namely AIa1, AIa2, AIb1, AIb2, AIb3, AIc, AId1, AId2 and AId3, have been separated and eluted in that order. Most of these minor hemoglobins were newly observed and could not be detected by the previous chromatographic techniques. On the basis of colorimetric assay protein-bound ketoamine was present in all the minor hemoglobins, which was confirmed by chromatographic separation of hemoglobins after reduction with NaB3H4. All the minor hemoglobins, with the exception of Hb AIa1, HbIa2, and Hb AIb1, showed a 2-fold increase in the diabetic patients. Hb AIc (5.0% in normals; 9.0% in diabetics) and Hb AId3 (1.9% in normals; 3.2% in diabetics) were present in the largest amounts. Both Hb AId3 and Hb Ao showed a decrease in oxygen affinity in the presence of 2,3-diphosphoglycerate, whereas Hb AIc showed no effect. Separation of globin chains by cellulose acetate electrophoresis at pH 8.6 showed that the cathodal mobility of the alpha chains of Hb AId3 was slower than those of Hb Ao. Glycosylation of Hb with [14C]glucose followed by separation of hemoglobins by two Bio-Rex 70 chromatographic methods indicated that the minor Hb formed by glycosylation of the alpha-chain amino-terminus was separated from Hb Ao, while the minor hemoglobins formed by the glycosylation of epsilon-NH2 groups chromatographed with Hb Ao.

Chromatography, Ion Exchange↗
PubMed1983

The effect of right temporal lobe lesions on matching of smells.

Thirty patients with bitemporal lobe epilepsy, 14 patients with right temporal lobe epilepsy, 14 patients with left temporal lobe epilepsy, 14 patients who had right temporal lobectomy, 10 patients who had left temporal lobectomy and 12 patients who had lobectomy involving frontal or fronto-parietal regions were tested for their ability to match smells. A simple matching technique was used. It was found that patients with right temporal lobe lesions had difficulty in matching smells. There was no significant difference between the right temporal lobe epilepsy group and the right temporal lobectomy group. The implications of these findings are discussed.

Discrimination Learning↗
PubMed1983

Affinity chromatographic quantitation of glycosylated hemoglobin in newborn infants.

Levels of glycosylated hemoglobin (glyco Hb) were determined in 45 newborn infants by affinity chromatography on phenylboronate-agarose gel and found to correlate well with the results obtained by two chemical methods. However the percentages of Hb FIa+b and Hb FIc determined by Bio-Rex 70 chromatography did not correlate with the glyco Hb values. The glyco Hb levels in the newborns were significantly lower than in the adults. Rechromatography of the glyco and nonglyco Hb fractions on Bio-Rex 70 columns indicated that Hb FO-like glyco Hb (presumably formed by glycosylation of the alpha chain amino terminus and epsilon-amino groups of lysyl amino acids) constituted about 60% of the glyco Hb fraction, whereas both Hb FIc and Hb FIa+b components contained only small portions of the glyco Hb.

Adult↗
PubMed1982

Modification of sickle hemoglobin by acetaldehyde and its effect on oxygenation, gelation and sickling.

Erythrocytes from sickle cell anemia patients and chromatographically purified Hb S0 were incubated with 0.25 to 120 mM acetaldehyde for 15 min to 6 h at different temperatures and pH. Several hemoglobin adducts stable to dialysis were separated by Biorex 70 chromatography and the proportions of the adducts formed were dependent on the period of incubation, acetaldehyde concentration, pH and temperature. Acetaldehyde treatment showed an increase in solubility, minimum gelling concentration and oxygen affinity and a decrease in sickling which showed a dependence on Hb S modification. With 0.25 to 1 mM acetaldehyde, significant inhibition of sickling was observed without any effect on the physical characteristics of the hemoglobin molecule. Acetaldehyde may act as a gelation inhibitor as well as a cell sickling inhibitor.

Acetaldehyde↗
PubMed1982

Synthesis of the minor fetal hemoglobin Fic in colonies of erythropoietic precursors isolated from human umbilical cord blood.

The biosynthesis of the minor hemoglobin FIc, which contains acetylated gamma chains, and the major hemoglobin Fo was studied during erythroid cell differentiation and maturation in cultures of erythroid precursors isolated from five human umbilical cord blood samples. A gradual decrease in the synthesis of Hb FIc was observed during the maturation of the erythroid cells when cultured from 7 to 15 days. The synthesis of Hb Fo did not show a consistent change; however, the relative synthesis of Hb FIc was the highest in the 7-day-old colonies and decreased at day 15 to levels observed in the reticulocytes. The addition of sodium butyrate, which is known to promote histone acetylation, significantly increased the synthesis of Hb FIc in 7-day-old colonies.

Butyrates↗
PubMed1982

Normal serum ferritin levels in a patient with HEMPAS syndrome and iron overload.

Serum ferritin levels in a patient with HEMPAS syndrome (hereditary erythroblastic multinuclearity associated with positive acidified serum test) were correlated with body iron stores directly measured on spleen and liver biopsy specimens as well as by quantitative serial phlebotomy. Normal serum ferritin concentrations were found in the presence of a moderate excess in iron stores (approximately 6-12 times normal). They temporarily increased after transfusion and splenectomy with a prompt return to the normal range. As repeated phlebotomies over a period of nine months depleted the excess iron stores, the serum ferritin ultimately decreased to a subnormal concentration. The serum ferritin concentration was not a reliable index of increased body iron stores in this iron overloaded patient, but did reflect their depletion by serial phlebotomy.

Anemia, Dyserythropoietic, Congenital↗