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Results for “vasculogenesis”

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5 recordsLinked to original sources
PubMed1978

The relation of aberrant vasculogenesis to skeletal malformation in the hamster fetus.

Oral administration of teratogenic doses of retinoic acid to pregnant hamsters on day 10 of gestation is associated with dysmorphogenesis of the appendicular skeleton. During the 24 h following retinoic acid treatment, the developing limb bud vasculature was disorganized, with blood vessels encroaching on areas where mesenchymal condensation of the skeletal blastemata normally occurs. Large, branching marginal folds and endothelial cell vesiculations protruded into the blood vessel lumina. It is suggested that the vascular changes observed may affect the concurrent early development of the skeleton and contribute to the skeletal malformation seen in near-term fetuses.

Abnormalities, Drug-Induced↗
PubMed2026

Live-cell transcriptomics with engineered virus-like particles.

Transcriptomic profiling is widely applied to characterize cellular gene expression, yet existing approaches lyse cells and preclude direct analysis of transcriptional dynamics in the same sample over time. We addressed this limitation by engineering mammalian cells to "self-report" their transcriptional states via mRNA export in virus-like particles (VLPs). Repeated sampling of culture media from VLP-producing cell populations faithfully captured evolving transcriptional states in complex biological settings, including acute inflammatory stimulation of primary cell spheroids and multi-day differentiation of pluripotent stem cells. We engineered VLP components for multiplexed readouts from distinct cell types in co-culture and for tuning self-reported RNA profiles. Finally, we demonstrated the unique utility of self-reporting for selective longitudinal tracking of endothelial cell dynamics within the enclosed architecture of a microphysiological co-culture system to identify perivascular stroma-dependent temporal gene programs underlying vasculogenesis. Altogether, this work establishes cellular self-reporting as a broadly enabling technology for live-cell transcriptome-wide gene expression profiling.

RNA↗
PubMed1978

An ultrastructural study of developing extracellular matrix in vitelline blood vessels of the early chick embryo.

This investigation was designed to describe the morphological events in embryonic development of peripheral blood vessels (vasculogenesis) and to relate this process to the appearance of extracellular matrix (ECM) during growth and maturation of these tissues. Extraembryonic vitelline vessels of the early chick embryo were chosen for this study and light, transmission, and scanning electron microscopy were carried out on vessels excised from chick embryos (Hamburger-Hamilton stages 8 through 23). Our data show that early (stage 10) vessels are composed of two distinct epithelial layers, an inner layer of presumptive endothelium surrounded by a layer of splanchnopleuric mesoderm. During development, the inner layer gives rise to mature vascular endothelium while splanchnopleuric mesoderm differentiates to form primitive vascular smooth muscle. Ultrastructural studies show the presence of collagen and basal lamina in the extracellular space between these two layers during initiation of endothelial and smooth muscle cytodifferentiation. Furthermore, ruthenium red-positive material is present on basal surfaces of developing vascular endothelium at this time, indicating possible glycosaminoglycans (GAG) or other polyanionic components of the ECM. These data suggest that the sequential production of basal lamina, collagen (s), and/or GAG's by developing peripheral vessel wall epithelia may be critical to their final differentiation.

Animals↗
PubMed1978

Development and ultrastructure of peripheral subretinal neovascularizations.

Anterior to the equator an additional vascular layer can regularly be found between peripheral retina and choroid. The vessels are located within Bruch's membrane, subjacent to the retinal pigment epithelium. Apparently, this layer is not present at birth and develops at a relatively young age. With the electron microscope all steps of vasculogenesis could be followed. The presence of such vessels also in the eye of a 29-year-old individual suggests that they represent a physiologic condition rather than an ageing change. In contrast to the subretinal neovascular membranes of the posterior fundus, these peripheral neovascularizations show no relation to any known fundus disease.

Adult↗
PubMed1979

[Correlations between the processes of cell differentiation and development of blood vessels in the (chick embryo) adenohypohhysis].

Utilizing semi-thin and, respectively, thick sections after indian ink injections, we have analyzed morphohistogenesis and vessel development in the adenohypophysis of chick embryos from the 4th to the 19th incubation day. Our preliminary results indicate that a close correlation exists between cellular differentiation and vasculogenesis. Vessels begin to enter the gland only at the 6th day when the first endocellular granules become detectable, and, from the 10th day, they markedly increase in number and size, gradually acquiring a peculiar sinusoidal arrangement around cellular groups, as cell differentiation further progresses.

Animals↗