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At least 19 recordsLinked to original sources

Blood groups and biochemical polymorphism in the Namaqua sheep breed.

The Namaqua is an indigenous fat-tailed African breed of sheep which has remained relatively isolated and which at one time dwindled to near extinction. Frequency data are given for blood group antigens, red cell glutathione and potassium types, for electrophoretic variants of red cell haemoglobin, 'X' protein, nucleoside phosphorylase, NADH-diaphorase, lysine and carbonic anhydrase and of plasma esterase, transferrin and albumin. Of particular interest was the occurrence of the i blood group, a bimodal distribution in red cell glutathione concentrations and red cell potassium concentrations of around 57 mmol/l cells, i.e. neither typically LK nor HK type.

Animals

Performance of three tropical hair sheep breeds.

The performance of three hair sheep breeds (Brazilian Somali, Morada Nova, and Santa Ines) was evaluated in the production environment of northeastern Brazil. Data from a total of 524 lambs sired by 21 rams and out of 190 ewes were analyzed. These data were collected from 1980 to 1983 at Sobral, Brazil from an experiment designed to compare performance of the three breeds. The traits included weights of lambs at birth, weaning, 8 mo, 10 mo, and yearling ages and ewe characters of weight at lambing (EWT), total lamb weight born (LWB), total lamb weight weaned (LWW), and prolificacy rate (PR). Differences (P < .01) among breeds were found for all characters. The Brazilian Somali, the smallest and slowest-gaining breed, was less sensitive to yearly variation than were the other breeds. The Santa Ines, the largest and fastest-gaining breed, was superior in LWB and LWW per ewe lambing. Averaged over the 4-yr period, the Morada Nova had the highest PR (1.82), and the Brazilian Somali and Santa Ines had similar PR (1.39 and 1.31, respectively). Environmental effects on PR due to yearly rainfall quantity and distribution pattern influenced lamb growth up to weaning and ewe reproduction performance. Breed x year (P < .05) interaction effects on PR were largely attributable to the Somali breed's exhibiting higher PR than the Santa Ines and Morada Nova breeds during the relatively wet years of 1981 and 1982.

Age Factors

Genetic variations in blood proteins within and between Spanish dairy sheep breeds.

Eleven flocks of three Spanish sheep breeds have been studied: four Churra, four Lacha and three Manchega. Genetic variations between flocks of the same and different breeds have been analysed, using eight genetic blood systems and a wide range of statistical methods. The results show that the Churra, Lacha and Manchega breeds present significant differences between each other. Significant intraracial variations have been observed in Churras and Lachas, although they were almost non-existent in Manchegas. In some cases the genetic distances between flocks of the same breed were greater than the distances between flocks of different breeds. The data indicate that the closest breeds genetically are Lacha and Manchega.

Animals

Genetic variation and distances of five Italian native sheep breeds.

Genetic distances among five of the most important Italian native sheep breeds were estimated by using gene frequencies of four blood group and seven blood protein loci and by three different computing methods. The values of the three distance matrices were highly correlated. Genetic distances were found within the range values reported in the literature for local breeds. Shorter distances fitted the historical evidence of migrations.

Animals

Hemotypes in Spanish sheep breeds.

The structure and the genetic diversity of the Churra, Lacha and Manchega sheep breeds have been analysed using hemotypes observed in eight loci. The three breeds are different in their hemotypes in terms both of quantity and quality. The proportions of unique hemotypes in Churra (60%), Lacha (61%) and Manchega (67%) revealed a high level of individual diversity within each breed. Racial genetic diversity follows the descending order of: Manchega-Lacha-Churra. The value of N:H (number of animals: total hemotypes) in the multi-racial population was 3.05.

Animals

Simulation of genetic and environmental interaction of three tropical hair sheep breeds.

The Texas A&M Sheep Simulation Model was used to examine genetic-environmental interactions (GEI) among Brazilian Somali, Morada Nova, and Santa Ines breeds and type and level of feed supplement in northeast Brazil. Two sets of simulations were conducted to examine effects of different supplement quality (napier grass vs cunha legume) and quantity (napier grass only). The analyses indicated the benefits from supplementing lambs at weaning during the dry season and from supplementing lactating ewes at the beginning of lambing season when forage is scarce. The cunha legume was an effective supplement, but choosing between cunha legume and napier grass is an economic consideration. Increasing the amount of napier grass supplement per ewe from 200 g/d to 400 g/d resulted in a significant GEI (i.e., different levels of response such as lamb mortality rate, lamb weaning weight, and lambing rate for different breeds). The Brazilian Somali was the least responsive, whereas the Santa Ines was the most responsive to supplementation. It was concluded that the level of supplement should be higher for the Santa Ines and Morada Nova than for the Somali. The amount of supplement should be regulated so that it neither substitutes for nor lowers the utilization of the native caatinga forage. None of the breeds was simulated to be superior in all environments. Thus, considering the wide range of resources of individual farmers, any one of these breeds may be the preferred choice, depending on the individual set of production resources.

Animal Feed

[Transplantation of embryos in sheep breeding].

Intra- and inter-breed transplantations were carried out with Bulgarian fresh embryos and with frozen embryos imported from Poland. A complex biotechnology was worked out, including methods of estrus synchronization, of inducing superovulation, and of producing, morphologic evaluation, storing, and transplantation of sheep embryos. Discussed are the possibilities of using embryo transplantation in sheep breeding for the fuller use of the reproductive potential of elite ewes as donors of embryos, for the introduction of new genotypes by means of importing frozen embryos as well as a modern model for fundamental investigations in the reproductive and cell biology.

Animals

Natural scrapie in British sheep: breeds, ages and PrP gene polymorphisms.

One hundred and sixty-seven sheep of 32 breeds and crossbreeds affected by natural scrapie throughout Britain were tested for the presence of restriction fragment length polymorphisms of the PrP gene observed when their DNA was digested with EcoRI or HindIII. These polymorphisms have already been associated with different susceptibilities to experimental scrapie (controlled by alleles of the Sip gene) in a flock of Cheviot sheep. In two studies 86 to 92 per cent of the sheep were found to carry the PrP gene EcoRI fragment e1 which is associated with high susceptibility (or the sA allele of Sip) to experimental scrapie. The PrP gene HindIII genotypes of the natural scrapie sheep were not apparently associated with differences in susceptibility to scrapie. There was no link between the polymorphisms and the age or breed of the affected sheep.

Age Factors

Large-scale low-coverage whole-genome sequencing reveals the genetic architecture of wool and growth traits in fine-wool sheep.

Breeding sheep with superior growth performance and wool quality is essential for the sustainability of the fine-wool sheep industry. In this study, we perform low-coverage whole-genome sequencing (lcWGS) on 3842 individuals from 5 sheep breeds (4 fine-wool and 1 semi-fine wool) and generate a large genomic dataset. By comparing these breeds with coarse-wool sheep, we characterize the genomic landscape and selection signatures of fine-wool sheep. We identify several known functional genes associated with hair follicle development and skin morphology, including EGFR, KRT74, EDAR, EREG, and GLI2. Furthermore, GWAS of 19 traits identifies 156 candidate genes significantly associated with growth and wool characteristics, including LCORL for body size, EGFR for clean wool yield, and PRDM1 for fiber diameter. Notably, EGFR is detected in both GWAS and selection signature analyses, indicating its important role in phenotype formation and historical selection. Overall, our findings reveal the genetic basis of growth and wool traits in fine-wool and semi-fine wool sheep, highlight EGFR, LCORL, and PRDM1 as candidate genes, and provide valuable genomic resources and candidate markers for future functional validation and molecular breeding.

Body size

The proposed use of melatonin in controlled sheep breeding.

The regulation by melatonin of hypothalamic-pituitary events in the ewe to advance seasonal oestrous activity, with no undesirable effects upon fertility, and its induction of those seasonal responses associated with short days indicates an essential role for melatonin in controlled-breeding programs in major sheep-producing countries. The development of suitable controlled-release systems to provide a choice of practical methods of melatonin delivery under field conditions is discussed as also are geographical and breed factors in controlled breeding with melatonin.

Animals

[The occurrence and distribution of maedi-visna virus infection in lower Austrian sheep breeding establishments].

In a period of one year blood samples of 374 sheep of Lower Austrian flocks were examined in intervals of two months for antibodies against Maedi-Visna (MV). Further on specific attention was paid to the prevalence of MV-infection in the flocks. The antibody-titers were determined by means of agar gel-immunodiffusion test. In 5 (23.8%) of 21 flocks 45 (12.0%) of the sheep were seropositive. Seroprevalence varied within the examined breeds: 19 (27.1%) of 70 Karakul-sheep, 18 (28.1%) of 64 East-Friesian milk-sheep, 7 (9.4%) of 74 Merino sheep and 1 (0.9%) of 111 mountain sheep. None of 55 black-headed sheep showed MV seropositive reaction. Clinical symptoms of Maedi-disease were especially observed in milk-sheep and for the first time in Austria in Karakul-sheep. Unfavorable housing conditions (insufficient room, bad climatic conditions), breed specific behavior patterns (crowding of Karakul-sheep) and a high prevalence of MV infections promote a high incidence the disease.

Animals

[Some parameters in reproduction of a Maroccain sheep breed (race d'Man) (author's transl)].

The importance of the sheep in Marocco was shown with help of statistical data. As there exist very few scientific work on sheep reproduction in this country, we began to work on local breeds with the race d'Man. The breed is asaisonal and is able to lamb 2 times within 13 months, with mostly 2 or more youngs (1,8 lambs per partum). The delay of gestation was found with 150 days, the duration of a cycle 17 days. The libido shown by the rams is violent. They easily accepted an other male as phantome for the collection of sperm with the easily accepted artificial vagina. The gonads are ovoid formed and are early developed. Remarkable is the tail of the epididymis which is covered in a pocketlike formed structure of the distal scrotum. Its volume is remarkable. Concerning the characteristics of the sperm there is no difference with other breeds. Therefore the conclusion seems reasonable that this apparent development of the epididmymis tail is an organ for thermoregulation for the sperm as the breeds origin are the oasis of the Sahara. The early onset of puberty as well as the violent sexual interest and the high rate of reproduction and asaisonal behavior could be a reference to a "primitive breed".

Animals

Genome-Wide SNP Characterisation of Three Kazakh Sheep Breeds: Kazakh Fat-Tailed Coarse-Wool, Degeres, and Etti Merino.

Kazakhstan's sheep portfolio underpins much of the country's mutton and wool production, yet several of its principal breeds remain genomically uncharacterised. The aim of this study was to characterise the genomic diversity, population structure, and global phylogenetic placement of three economically important Kazakh breeds and to determine whether they constitute separate gene pools requiring independent management. We present the first genome-wide SNP characterisation to include the Degeres (DE), the Etti Merino (EM), and the Kazakh fat-tailed coarse-wool (KKG) breeds simultaneously. A total of 1497 animals (DE = 354, EM = 642, KKG = 501) sampled across seven production households were genotyped and, after quality control, analysed at 42,279 SNPs, of which 22,766 LD-pruned markers were used for principal component analysis and AMOVA. We applied principal component analysis (PCA), pairwise FST, analysis of molecular variance (AMOVA), neighbour-joining phylogenetics, model-based ancestry estimation (ADMIXTURE), and Hill-number diversity profiling, and projected the breeds against the global Ovine SNP50 HapMap panel (74 reference breeds, 2819 animals; 37,685 shared SNPs). All three breeds retained uniformly high within-breed diversity (expected heterozygosity 0.413-0.417) with fixation indices at or near zero. AMOVA partitioned 94.03% of variance within breeds (&#x3a6;ST = 0.060, p < 0.001). PCA, phylogeny, and ADMIXTURE concordantly resolved three breed-specific clusters at K = 3, with a maximum interbreed FST of 0.038 within the study dataset. Against the global panel, EM was genetically closest to Merino and Merino-derived reference breeds (pooled FST = 0.017) and substantially more distant from Southwest Asian sheep (FST = 0.045), whereas DE and KKG showed the reciprocal pattern (FST = 0.027 and 0.020 to Southwest Asia, 0.052 to the Merino group). DE additionally displayed the heterozygote excess and partial admixture expected of an incompletely consolidated composite. These results delineate three distinct gene pools and carry direct implications for breed management and the conservation of genomic diversity in Kazakhstani sheep.

ADMIXTURE

[Blood group systems of Caucasian-breed sheep].

Reagents for six genetic systems of blood groups are obtained and tested on the international scale. For A-system: anti-Aa and Ab for B-system: anti-Bb, Bc, Bg, Bi, Be, Bd; for other systems anti-Ca, Cb, Ma, Da sera are obtained. Antibodies of the R-system are isolated from normal sera of the sheep and goat blood. Antigen frequency is studied for eight systems of blood groups in 205 Caucasian sheep.

Animals

Controlled sheep breeding: update 1980-1985.

This contribution to the Symposium concerns four topics which have been addressed in our laboratory over the past five years. First, the responses to a controlled light environment of Merino ewes and rams have been compared with those of two British breeds. The endocrinological patterns were similar in all breeds but cyclic ovarian activity and ram libido were different. While showing a degree of entrainment to photoperiod, the breeding patterns were much less rigidly controlled in the Merinos than in the others. Second, the effectiveness of establishment of a cervical reservoir of spermatozoa, in ewes in which oestrus and ovulation have been controlled, has been re-examined. This is highly dependent on the time of insemination relative to that of the release of LH. Maximum numbers are found when ewes are inseminated shortly after the LH peak, i.e. some 6-10 h after the onset of oestrus. Third, the quantitative and temporal endocrinological and behavioural events following standard, progestagen-PMSG treatment have been quantified. Contrary to earlier expressed beliefs, these events are remarkably predictable provided an intensive system of mating or detection of oestrus is used. The onset of oestrus in treated anoestrous crossbred ewes has a normal distribution, with a range of 24 h, centred around a mean of 33 h after withdrawal of a 30 mg Cronolone intravaginal sponge and injection of 500 i.u. PMSG. This period of time is dose-dependent. The LH peak occurs 4.5 +/- 0.7 h later and the times of onset of oestrus and of LH release are highly correlated (r = 0.93). Ovulation is some 24 h later again. Fourth, differences in the response of ewes to different batches of PMSG have been defined. While the three commercial preparations studied regularly induced ovulation in anoestrous ewes at doses of 250 i.u. and above, the quantitative responses varied greatly. One preparation would not induce multiple ovulation, even at high doses. There are differences in steroidogenesis and in pregnancy rates, associated with dose of PMSG and the consequent ovulation rate: the ideal would be for every ewe to shed two or three ova. A higher ovulation rate is acceptable, as early embryonic mortality generally reduces the litter size. This is particularly important in deep anoestrus. However, this does not solve the problem of breeding in early lactation.

Animals