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At least 19 recordsLinked to original sources

Lamb production and its components in pure breeds and composite lines. II. Breed effects and heterosis.

Ewe performance was compared for five pure breeds of sheep (Finnsheep, F; Rambouillet, R; Dorset, D; Targhee, T; Suffolk, S) and the crosses in development of two maternal composite lines (C1 = 1/2F1/4R1/4D and C2 = 1/2F1/4T1/4S). The data involved 10,959 breeding season records of 4,219 ewes by 412 sires. Ewe production and the components (fertility, litter size, neonatal and preweaning lamb survival and mean lamb weaning weight) were adjusted for age and standardized across season of lambing and years. The D and F ewes produced more weight of lamb/ewe exposed than R, S and T ewes because of higher D and F fertility, higher D lamb survival and larger F litters. First cross and inter se generations of C1 and C2 ewes averaged 17 to 27% higher fertility than the parental mean. Litter size averaged about one lamb higher for F than for other pure breeds, but only slightly higher for C1 and C2 than for the mean of F and other breeds. Lower neonatal survival for F than for other breeds and crosses was associated with the larger F litters and with 2 to 8% positive heterosis in the crosses. Preweaning survival of suckled and nursery lambs was low for F and S and positive heterosis ranged from 9 to 19% in crosses. Mean lamb weaning weights were highest for S, lowest for F, with little heterosis in crosses. Crossbred ewes reared .3 to .4 more lambs than mean for parental pure breeds. Heterosis in C1 and C2 ranged from 11 to 28% for lambs born, 27 to 43% for lambs weaned and 29 to 44% for weight of lamb weaned/ewe exposed. Decline in heterosis with inter se mating of crosses was no greater than expected from the reduction in predicted heterozygosity.

Animals↗

Inheritance of reproductive traits of female common ducks (Anas platyrhynchos) in pure breeding and in inter-generic crossbreeding with muscovy ducks (Cairina moschata).

1. Genetic parameters of reproductive traits were estimated in a population of common duck, in purebreeding and crossbreeding (with Muscovies) insemination systems. A total of 989 females were studied over three generations as well as 4025 purebred offspring and 4,125 male mule offspring. 2. Traits studied were age at first egg, total number of eggs laid until the age of 48 weeks, fertility and hatchability rates in pure and crossbreds, weight at 6 and 30 weeks of age, average egg weight and body weight of the male mule ducks at 6 weeks of age. 3. Heritability estimates were found to be medium range for reproductive traits (0.15 to 0.47). Heritability value for fertility or hatchability in crossbreds was twice as high as in purebreds (0.32 vs 0.15 for fertility; 0.36 vs 0.16 for hatchability). 4. Fertility in purebreeding and in crossbreeding were two different traits (r(g) = 0.49) while hatchability displayed a high genetic correlation between breeding systems (r(g) = 0.88). 5. Genetic correlations with number of hatched mule ducks were medium or high and favourable. Genetic correlations between reproductive traits and weights were low (< 0.36), the most related trait being the body weight of the male mule duck at 6 weeks of age.

Animals↗

Equine leukocyte antigens: relationships with sarcoid tumors and laminitis in two pure breeds.

Frequencies of equine leukocyte antigen distribution were determined by complement-mediated cytotoxicity testing among populations of Thoroughbred and Standardbred horses, including animals affected with equine sarcoid and laminitis. A highly significant association is described between the presence or history of sarcoid lesions in Thoroughbreds and the expression of the major histocompatibility complex (MHC)-encoded antigens, W3 and B1. No association was found between antigenic expression frequencies and laminitis in either breed. These findings suggest that a strong relationship exists between the equine MHC and a predisposition to sarcoid.

Animals↗

Lamb production and its components in pure breeds and composite lines. I. Seasonal and other environmental effects.

Season of lambing and other environmental effects on ewe performance were analyzed for purebred Finnsheep (F), Rambouillet (R), Dorset (D), Targhee (T) and Suffolk (S) and the generations of crosses in development of two maternal composite lines (1/2F1/4R1/4D) and (1/2F1/4T1/4S) in accelerated (January, May and September) or annual April lambing. The data involved 10,959 ewe breeding season records for 4,219 ewes of 412 sire families over 4 yr. Various measures of ewe productivity and its components (fertility, litter size, neonatal and preweaning survival and weaning weight) were analyzed. Fertility was higher for annual April than for accelerated May or January lambing and was sharply lower for September lambing. Fertility of F and F-cross ewes was significantly higher for May and lower for January lambings relative to R and D ewes. Litter size also was higher in annual April (1.9) than in January (1.8) or May (1.7) and September (1.4). Neonatal and preweaning survival was higher in September when litter size was smaller. Mean weaning weights were depressed in the larger April and May litters. Thus, weight of lambs weaned/ewe exposed was higher for annual April than for May and January lambing and was very low for September lambing. Hormone treatment of ewes for September lambing increased fertility from 16 to 44% and litter size from 1.6 to 1.8. Short lambing interval (8 mo vs 12+ mo) reduced mean ewe fertility by five percentage points, and most for the January lambing of Finnsheep ewes.

Animals↗

[Use of a PCR method for controlling pure-breeding of honeybees Apis mellifera mellifera L. in the southern Urals].

A key problem of honeybee (Apis mellifera mellifera) breeding in the Southern Urals is its cross-breeding with the Caucasian honeybee Apis mellifera caucasica. Mitochondrial DNA (mtDNA) in these subspecies differ in the length of a fragment localized between genes CO-I and CO-II, which can be used as a marker. A pair of 20-mer primers for PCR was chosen by means of computer design in order to determine the fragment size in both of the subspecies. The amplified fragment was shown to have a length of 350 bp in A. m. caucasica and 600 bp in A. m. mellifera. The difference in length results from the different ratio between two main elements P and Q, which comprise a major part of this sequence in these subspecies: a copy of P element and two copies of Q element in A. m. mellifera, and a copy of Q element only in A. m. caucasica. This sharply defined distinction allows us to use PCR for differentiating the subspecies, estimating the heterogeneity in the colonies, and rejecting queens in the selection process because of the maternal inheritance of the studied character. The nucleotide sequence of the amplified mtDNA fragment of A. m. mellifera was determined.

Amino Acid Sequence↗

An epidemiological study of gastrointestinal parasites of dogs from Southern Greater Buenos Aires (Argentina): age, gender, breed, mixed infections, and seasonal and spatial patterns.

A total of 2193 fecal samples from owned dogs were collected during the 2003-2004 period in Southern Greater Buenos Aires, and were evaluated for the presence of intestinal parasites by a flotation-centrifugation method. The overall prevalence was 52.4%, and the 11 species found were: Ancylostoma caninum (13%), Isospora ohioensis complex (12%), Toxocara canis (11%), Trichuris vulpis (10%), Sarcocystis sp. (10%), Giardia duodenalis (9%), Isospora canis (3%), Hammondia-Neospora complex (3%), Dipilydium caninum (18 cases), Cryptosporidium sp. (5 cases), and Toxascaris leonina (1 case). There was no significant difference in the overall prevalence between genders (female = 50.4%, male = 54.6%), and breeds (pure = 52.3%, mixed = 53%), but prevalence in puppies (<1 year) was higher than in adult dogs (62.7% versus 40.8%, respectively). Only the prevalence of A. caninum differed between genders, with higher values for males. The prevalences of six of the parasite species showed a decreasing trend with increasing host age, and an inverse pattern was found for two other species. The prevalences of three protozoa were significantly higher in pure-breed dogs, and those of two nematodes were significantly higher in mixed-breed dogs. The prevalences of T. canis, A. caninum, and T. vulpis were spatially heterogeneous with a clear Southwest-Northeast gradient. Only prevalences of Sarcocystis sp. and G. duodenalis showed seasonal variation. The frequency distribution of the number of species per fecal sample did not differ from a random distribution. Results obtained throughout the world were discussed.

Age Factors↗

Electrocardiographic observation on spontaneously occurring arrhythmias in chickens.

In order to clarify the spontaneous occurrence of arrhythmias in chickens, electrocardiograms using standard bipolar limb leads were recorded for 461 chickens of 7 breeds, including 3 pure breeds: Rhode Island Red (RIR, n = 125), Japanese Game (JG, n = 101), and White Leghorn (WL, n = 52); and 4 crossbreeds of RIR: the first filial generation (F1) obtained by mating RIR with JG (RIR x JG, n = 44), the backcross obtained by mating the Fl of RIR x JG with the parental JG ((RIR x JG) x JG, n = 10), the F1 obtained by mating RIR with WL (RIR x WL, n = 73), and the F1 obtained by mating RIR with the commercial hybrid chicken, Dekalb Amber Link (DAL)(RIR x DAL, n = 56). Nine types of arrhythmias were observed in 107 chickens (23.2%, 107/461): 66 had sinus arrhythmia, 19 had atrial premature contraction, 9 had ventricular preexcitation syndrome, 7 had ventricular premature contraction, 2 had second-degree atrioventricular block. 1 had atrial fibrillation, I had aberrant ventricular conduction. I had intraventricular conduction disturbance, and 1 had ventricular electrical alternans. Except for sinus arrhythmia, the incidence of other arrhythmias in RIR was significantly high, compared with that in pure breeds other than RIR (p < 0.001) and that in crossbreeds of RIR (p < 0.01). Arrhythmias other than ventricular premature contraction and sinus arrhythmia will be the first to be described as spontaneously occurring arrhythmias in chicken. Although the reason for both the highest incidence of sinus arrhythmia in WL and the predominance of other arrhythmias in RIR were obscure, the present results suggest the possibility of using chickens, especially RIR which shows a high incidence of arrhythmias, as a relevant animal for studying arrhythmias.

Animals↗

Direct and maternal genetic covariances by age of dam for weaning weight.

Weaning weights of calves of dams at ages in years of 2, 3, and older were modeled to be three separate traits. Fixed effects were sex of calf-year of birth combinations for nine pure breeds and sex of calf-year of birth-generation for three composite populations. Random effects fitted for each trait were correlated direct and maternal genetic, maternal permanent environmental, and temporary environmental. Direct and maternal effects were correlated across traits. A multiple-trait, derivative-free REML algorithm was used to estimate the 30 (co)variance components. Number of animals per breed group ranged from 1,244 to 4,326. For the three traits for pure breeds, average proportions of phenotypic variance were .34, .31, and .27 for direct genetic; .16, .15, and .12 for maternal genetic; and .18, .20, and .17 for maternal environmental effects. Average correlations among the three traits were .84 for direct genetic, .78 for maternal genetic, and .71 for maternal environmental effects. Average of direct-maternal genetic correlations for pure breeds was .05. For the composite breeds, average proportions of phenotypic variances were .44, .46, and .36 for direct genetic; .06, .06, and .05 for maternal genetic; and .16, .14, and .14 for maternal environmental effects. Average correlations among the three traits were .93 for direct genetic, .76 for maternal genetic, and .85 for maternal environmental effects. Average direct-maternal genetic correlation was .09 for composites. No evidence was found for greater direct-maternal genetic correlation for earlier than for later ages of dam. Sign and magnitudes of direct-maternal genetic correlations seemed to differ among pure breeds and were reflected in composites from those parent breeds.

Aging↗

Ownership pattern and management practices of cattle herds in the Gambia: implications for a breeding programme.

A questionnaire and a census were carried out in 1998/99 on 201 cattle herds in 44 villages located in three different areas of The Gambia, in order to obtain information about the ownership pattern, management and breeding practices for optimizing and extending a pure breeding scheme for indigenous N'Dama cattle. The herds had an average of 58.5 head, of which 39.3% were cows, and most of them were multiowner herds, having on average 5.5 owners. Overall, women represented 38.3% of the owners, but they owned only 15.9% of the cattle. Some of the senior herdsmen received no payment, as they were family members, but most of them received payment in kind, i.e. milk off-take from the herd. Payment in cash only or cash as well as milk was rare. In larger herds it was quite common for additional herdsmen to be hired throughout the year, their payment shifting towards cash only or cash and milk. Many small herds had no older bulls or breeding bull and almost all breeding bulls were born within the herd. In most cases, it was the herd owner who was responsible for the selection of the breeding bull. Traits related to the growth and milk production of the dam were considered as most important for a good breeding bull. The results are discussed with respect to the extension of a pure breeding scheme for N'Dama cattle.

Animal Husbandry↗

Haemoglobins in cattle and buffalo. Haemoglobin types of Bos taurus, Bos indicus, Bos banteng and Bubalis bubalis in northern Australia.

The frequency of haemoglobin types in several major breeds of cattle in northern Austrailia was determined. In all Boss taurus cattle examined only the three common bovine haemoglobin types (AA, AB, BB) were found. F2 Africander cross-breeds showed only AA patterns. The frequency of haemoglobin B was significantly higher in Bos indicus type cattle than in Bos taurus breeds. In the pure breed Banteng cattle (Bos banteng) three genotypes (BB, CB, CC) were present. The eleven buffaloes types each showed two haemoglobins (A1 and A2) in proporotions of 71 to 29 respectively.

Animals↗

Assessing the efficacy of a ram-genotyping programme to reduce susceptibility to scrapie in Great Britain.

Susceptibility to clinical scrapie is associated with polymorphisms in the PrP gene. The 'ARR' allele of this gene reduces susceptibility to clinical disease caused by all known strains of the transmissible spongiform encephalopathy (TSE) agent. The British government proposes to use a ram-genotyping scheme to breed genetic resistance to clinical scrapie into the national sheep population. We considered how best to target limited genotyping resources to achieve the maximum rate of genotype evolution. We created a metapopulation model of the British sheep industry, which includes the major pure-breeds of sheep and the cross-breeds produced by crossing these pure-bred animals. The main criterion for assessing the efficacy of different strategies was the time taken to increase the prevalence of the ARR allele in the slaughter-lamb population. Our model predicted that the most-effective strategy would be to target genotyping to those rams used for pure-breeding (i.e. mated with the same breed of ewe). This strategy was compared to two further strategies, in which the proportion of rams genotyped in each breed depended on the prevalence of the ARR/ARR genotype in that breed. A policy in which the proportion of animals genotyped is reduced as the ARR prevalence in that breed increases is efficient. The most-effective policy was targeting the hill sector in the early years and gradually switching to genotyping more terminal-sire and longwool rams as the resistance of the hill sector increases.

Animal Husbandry↗

Variability of canine microsatellites within and between different dog breeds.

Polymorphic animal microsatellites have proved valuable genetic markers. For this project, the variability of 19 canine microsatellite loci was examined within and between three pure breeds of dog: Greyhounds, Labradors, and German Shepherds. The number of alleles, absolute and relative frequencies, and the statistics that express polymorphism within a breed were determined. The evolutionary relationships among these closely related dog breeds were estimated by genetic distance measures developed for use with microsatellite loci. According to the pairwise genetic distances, Greyhounds and German Shepherds had longer diverse evolutionary histories than Greyhounds and Labradors or Labradors and German Shepherds. Although a few breed-specific alleles were observed, the significant differences between breeds are in their relative frequencies and distribution of the alleles across a locus. None of the three pure dog breeds corresponds to Hardy-Weinberg equilibrium. A considerable reduction in intrapopulation variation was observed within three pure breeds, compared with the population of individuals belonging to 15 dog breeds. This reduction was especially pronounced in the Greyhound breed, which expressed the lowest degree of variation. Intrapopulation variations of Labradors and German Shepherds did not differ significantly, that of Labradors being only slightly higher. The intra-species variation of dogs is lower than in humans, mouse, or rat, but similar to that in domestic animals, probably reflecting similarly high inbreeding coefficients. However, some highly informative loci were common to all dog breeds tested so far. Such population data are necessary for mapping studies and linkage analysis in dogs.

Alleles↗

Genetic evaluation by best linear unbiased prediction using marker and trait information in a multibreed population.

Genetic evaluation by best linear unbiased prediction (BLUP) requires modeling genetic means, variances, and covariances. This paper presents theory to model means, variances, and covariances in a multibreed population, given marker and breed information, in the presence of gametic disequilibrium between the marker locus (ML) and linked quantitative trait locus (MQTL). Theory and algorithms are presented to construct the matrix of conditional covariances between relatives (Gv) for the MQTL effects in a multibreed population and to obtain the inverse of Gv efficiently. Theory presented here accounts for heterogeneity of variances among pure breeds and for segregation variances between pure breeds. A numerical example was used to illustrate how the theory and algorithms can be used for genetic evaluation by BLUP using marker and trait information in a multibreed population.

Genetic Linkage↗