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3 recordsLinked to original sources
PubMed2026

Prevalence, Species Diversity, and Molecular Characterization of Viruses Infecting Phlox (Phlox paniculata) from Botanical Gardens in Russia.

Phlox (Phlox spp., Polemoniaceae) are herbaceous ornamental plants that are cultivated around the world for their fascinating flowering. P. paniculata cultivar collections at the Tsitsin Main Botanical Garden and the Botanical Garden of Lomonosov Moscow State University (both in Moscow, Russia) were surveyed for virus diseases using high-throughput sequencing and RT-PCR. Twenty-seven plants with virus-like symptoms on the leaves were selected for analysis. Alfalfa mosaic virus (AMV), Arabis mosaic virus (ArMV), ArMV satellite RNA (satRNA), beet ringspot nepovirus (BRSV), BRSV satRNA, cucumber mosaic virus (CMV), Spiranthes mosaic virus 3 (SpiMV3), tobacco streak virus (TSV), and tobacco rattle virus (TRV) were identified. AMV and TSV were the most common viruses found in 22 and 25 out of the 27 samples tested, respectively. ArMV satRNA was detected in phlox for the first time, adding to the list of viruses infecting this crop. The nearly complete genomes of the detected viruses and satRNAs were assembled and characterized. The CMV and TRV genome sequences from phlox were first deposited in GenBank. Only RNA1 was detected in the TRV-infected 'Fligelleutenant Bolke' cultivar plant, suggesting that the so-called non-multiplying infection has been found for the first time in a plant species other than solanaceae.

Arabis mosaic virus↗
PubMed1977

[Satellite RNA (RNA3) of tomato black ring virus is found with one of the 2 major RNAs (RNA2) in a new capsid nucleoprotein].

Tomato Black Ring Virus (TBRV) like other NEPOviruses posseses two nucleoproteins M and B and two major RNAs, RNA1 and RNA2 respectively distributed in B and M. A new nucleoprotein has just been discovered and comprises one molecule of RNA2 associated with one molecule of RNA3. RNA3 is a small RNA of molecular weight 500,000 d considered to be a satellite RNA. Its level appears to depend on the infection stage, local or systemic. RNA3 is able to modify the relative proportions of nucleoproteins M and B and their respective RNAs. The satellite RNA, might be part of the genome and represent a monocistronic mRNA for protein capsid synthesis. However it seems perhaps more tempting to correlate TBRV-RNA3 with satellite RNA5 of certain strains of Cucumber mosaic virus.

Capsid↗
PubMed2026

Virome metatranscriptomic profiling of birch pollen reveals a diverse viral community.

INTRODUCTION: Viruses are increasingly recognized as integral components of plant-associated biological systems. However, their occurrence and diversity in the reproductive tissues of woody plants remain poorly understood. Birch (Betula spp.) produces large quantities of wind-dispersed pollen that can travel over long distances and may harbour viruses or virus-derived nucleic acids originating from the host plant and/or its associated microbiota. METHODS: We investigated the virome of birch pollen collected from trees growing in central and suburban Berlin, Germany. Metatranscriptomic analyses were performed on pooled pollen samples collected in 2020. These analyses were complemented by RT-PCR screening of individually processed pollen samples collected in 2025 from resampled trees. Primer walking was additionally used to recover an extended genome sequence of a pollen-associated birch toti-like virus. RESULTS: Multiple virus-associated contigs were identified in both pooled metatranscriptomic datasets. These included sequences corresponding to the cherry leaf roll virus (CLRV), the birch idaeovirus (BIV) and the birch toti-like virus (BTLV), as well as additional virus-like contigs provisionally assigned to lineages related to the Orthototiviridae, Botourmiaviridae, Endornaviridae, and Chrysoviridae families. RT-PCR analysis of individually processed pollen samples confirmed the continued detection of CLRV, BIV, and BTLV within the Berlin sampling framework. A near-complete genome sequence was recovered from a pollen-derived BTLV isolate from Berlin, showing high amino acid sequence identity to a recently described leaf-derived BTLV isolate from the United States. DISCUSSION: These findings demonstrate that birch pollen harbours a diverse assemblage of plant- and/or microbiome-associated viruses and expand the known tissue distribution and geographic range of BTLV. More broadly, they establish pollen as an underexplored ecological niche for virome research and provide a foundation for future studies on the ecology, transmission dynamics, and epidemiological significance of pollen-associated and pollen-transmitted viruses.

Betula↗