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At least 19 recordsLinked to original sources
PubMedDate not supplied

[Functional connections between the suprapharyngeal ganglion and segmental motor centers of the cockroach Periplaneta americana].

Studies have been made of non-specific influences of supraesophageal ganglion on the excitability of the segmental centers in the cockroach P. americana. It was shown that electrical stimulation of the mushroom bodies decreases the frequency of the background discharges which are observed in the neuropile of the central body, decreasing simultaneously the activity of motor fibers of the Vth nerve. On the contrary, stimulation of the central body increases the frequency of the spontaneous activity in motor output. It is suggested that the inhibitory influences from the mushroom bodies upon the segmental motor apparatus may spread both directly and in a stepwise fashion, i. e. via the intermediate structure of the central body. The latter is involved into the control of muscle tonus in the cockroach.

Animals↗
PubMed1977

Origin, destination and mapping of tritocerebral neurons of locust.

The connectivities of the tritocerebrum of locust (Locusta migratoria L., Schistocerca gregaria (Forsk.)) were studied histologically and by means of cobalt chloride infusion. Its neuropil consists partly of fibers which traverse the tritocerebrum and areas consisting of neuropilar agglomerizations ("glomeruli"). The following direct connections between the tritocerebrum and other regions were observed: connections to 1) dorsal and lateral brain regions (mushroom body, optic lobe), 2) the ventral nerve cord, 3) the stomatogastric nervous system (here the protocerebrum and the subesophageal ganglion are also involved in these connections), 4) the retro-cerebral glands (corpora cardiaca, corpora allata), and 5) muscles of the foregut.

Animals↗
PubMed1975

Cobalt sulphide staining of optic fibres in the brain of the cricket, Gryllus campestris.

Neuronal projections from one optic lobe to other parts of the brain were stained in the cricket Gryllus campestris using the cobalt sulphide technique and Timm's sulphide-silver method. The results are: Four tracts directly connect the medulla with the lobula and medulla of the contralateral optic lobe. Direct medullar projections end mainly in the non-glomerular neuropile of the protocerebrum, but also penetrate the calyx of the mushroom bodies, pons and central body in small numbers. A few somata which send fibres into the medulla lie in the pars intercerebralis, in the protocerebrum ventral to the opposite beta-lobe, the outer margin of the medulla of the contralateral optic lobe and between deuto- and tritocerebrum. The anatomical and physiological relevance of the stained connections is discussed.

Animals↗
PubMed2023

A systematic CRISPR screen reveals redundant and specific roles for Dscam1 isoform diversity in neuronal wiring.

Drosophila melanogaster Down syndrome cell adhesion molecule 1 (Dscam1) encodes 19,008 diverse ectodomain isoforms via the alternative splicing of exon 4, 6, and 9 clusters. However, whether individual isoforms or exon clusters have specific significance is unclear. Here, using phenotype-diversity correlation analysis, we reveal the redundant and specific roles of Dscam1 diversity in neuronal wiring. A series of deletion mutations were performed from the endogenous locus harboring exon 4, 6, or 9 clusters, reducing to 396 to 18,612 potential ectodomain isoforms. Of the 3 types of neurons assessed, dendrite self/non-self discrimination required a minimum number of isoforms (approximately 2,000), independent of exon clusters or isoforms. In contrast, normal axon patterning in the mushroom body and mechanosensory neurons requires many more isoforms that tend to associate with specific exon clusters or isoforms. We conclude that the role of the Dscam1 diversity in dendrite self/non-self discrimination is nonspecifically mediated by its isoform diversity. In contrast, a separate role requires variable domain- or isoform-related functions and is essential for other neurodevelopmental contexts, such as axonal growth and branching. Our findings shed new light on a general principle for the role of Dscam1 diversity in neuronal wiring.

Animals↗
PubMed2025

Learning neural dynamics through instructive signals.

Rapid learning is essential for flexible behavior, but its basis in the brain remains unknown. Here we introduce the PRISM plasticity rule, a unifying mechanistic model of three well-established, fast-acting synaptic plasticity rules-in hippocampus, cerebellum and mushroom body-which relies exclusively on pre-synaptic activity and an "instructive signal" from another brain area. Using a multi-region network model we show that guiding PRISM plasticity with instructive signals enables the network to quickly learn extremely flexible nonlinear dynamics underlying behaviorally relevant computations, as well as to emulate unknown external system dynamics from real-time error signals, which we demonstrate with comprehensive simulations supported by exact mathematical theory. Thus, PRISM plasticity guided by instructive signals is well-suited to rapidly learn general-purpose neural computations-in contrast to canonical Hebbian rules. Finally, we show how including this plasticity rule in artificial learning algorithms can solve long-range temporal credit assignment, a long-standing challenge in machine learning.

cerebellum↗
PubMed1976

beta-Mannosidase from the mushroom Polyporus sulfureus.

beta-D-Mannosidase (EC 3.2.1.25), a useful tool for the structural studies of heterosaccharide chains, has been isolated in a highly purified form from the fruiting bodies of the mushroom Polyporus sulfureus. This mushroom is unique among reported sources of this enzyme in that it has the advantage of being almost free of alpha-mannosidase activity. The purification procedure involves ammonium sulfate fractionation followed by Sephadex G-100 filtration and chromatography on columns of DEAE-cellulose and hydroxylapatite. The final enzyme preparation gives essentially a single band on disc gel electrophoresis. The purified enzyme liberates the beta-D-mannopyranosyl unit from various natural substrates such as the core glycopeptide, Man(GlcNAc)2-Asn isolated from ovalbumin, from Taka-amylase A, and from human alpha1-acid glycoprotein. It also hydrolyzes (Man)2-GlcNAc from the urine of an alpha-mannosidosis patient, 1,4-D-mannobiose and mannotriose isolated from ivory nut mannan, 4-O-beta-D-mannopyranosyl-L-rhamnose, 6-O-beta-D-mannopyranosyl-D-galactose and 4-O-beta-D-mannopyranosyl-N-acetylglucosamine. The molecular weight of this enzyme is estimated to be about 64,000 by gel filtration. For p-nitrophenyl-beta-D-mannopyranoside, the pH optimum is between 2.4 and 3.4 and the Km is 1.6 mM.

Basidiomycota↗
PubMed1978

[The potassium content of mushrooms (author's transl)].

The potassium content of 410 species (1075 samples) of wild mushrooms ranged between 1.5 and 117g/kg dry wt. It was dependent on species and genus, and on the average was highest in Coprinaceae and lowest in Polyporaceae. A positive correlation was observed between potassium and water content; consequently, the potassium content of the wet wt. showed less variation: it ranged between 0.6 and 14.6 g/kg. In the solid matter of single fruit-bodies (11 species) potassium was distributed as follows: flesh of the cap greater than stem greater than gills or tubes greater than spores. The flesh of the cap contained maximally 1.8 times the potassium of the gills. But due to a correspondingly higher water content, significant potassium differences between fresh weights of these parts of fruit-bodies were uncommon. Potassium was concentrated 20--40-fold in fruit-bodies as compared with the soil.--According to our results mushrooms rank among the most potassium-rich vegetables, appropriate for consideration in diets in chronic potassium deficiency. On the other hand, toxic effects must be considered, especially in cases of renal insufficiency.

Basidiomycota↗
PubMed1978

Differences in normal structure and reaction to adjuvant between the costal and the visceral pleura.

Visceral and costal pleurae of the rabbit were studied by light and electron microscopy in the normal state, after India ink injection into the pleural cavity and in pleuritis induced by intrapleurally injected adjuvant. In normal rabbits, the mesothelial cells in the visceral pleura had more numerous microvilli and pinocytotic vesicles than in the costal pleura. The surface coat on the microvilli was much thicker in the former than in the latter. Intrapleurally injected India ink particles were phagocytosed by mesothelial cells and macrophages. The phagocytotic activity was more prominent on the costal side than on the visceral side. In rabbits with adjuvant induced pleuritis, the visceral pleura, but not the costal pleura, showed mushroom-like projections on the pleural surface which were composed of a fibrin mass mixed with phagocytotic macrophages and covered by proliferative mesothelial cells. These hitherto poorly known structures, which were formed, though less conspicuously, also after India ink administration, seemed to play an important role in the initial protection of the lung against foreign body invasions from the pleural cavity. With the progress of pleuritis, the mushroom-like projections changed into patchy granulation tissues which were well localized and did not exceed the elastic layer. In the costal pleura, apparently due to a poorer protective mechanism than in the visceral pleura, granulation tissue was formed much more extensively and diffusely. No signs of transformation of mesothelial cells into fibroblasts were recognized. Fat-containing cells closely resembling the Ito cells in the liver occurred in the submesothelial and subpleural layers of the visceral pleura.

Acid Phosphatase↗
PubMedDate not supplied

Damage caused by two finnish mushrooms, Cortinarius speciosissimus and Cortinarius gentilis on the rat kidney.

The toxicity of two mushroom species found in Finland, Cortinarius speciosissimus and Cortinarius gentilis, on the rat was studied. Dried, homogenized mushroom was given orally by stomach tubing. A dose of 500 mg dried mushroom/kg body weight, was used. It was demonstrated that both species caused renal damage. No damage could be shown in other organs. The renal histopathological changes corresponded to those of tubulo-interstitial nephritis. The sensitivity of different individuals to the fungal toxins varied greatly.

Animals↗
PubMedDate not supplied

Sex differences in renal damage induced in the mouse by Amanita virosa.

The sensitivity of male and female mice to Amanita virosa was compared. Dried, homogenized mushroom was given orally by stomach tubing at doses of 100, 200, 400 and 800 mg dried mushroom/kg body weight. Both in males and in females, the kidneys were the only organs showing macroscopical changes. The dose of 100 mg/kg caused renal damage in females, whereas in males the first signs of kidney damage were seen at the dose of 400 mg/kg. The renal lesions observed in the males were located in the cortex, while in the females they were limited to the outer stripe of the outer medullary zone. Testectomy diminished the nephrotoxicity of A. virosa in male mice and caused changes in the localization of renal lesions.

Amanita↗
PubMed1977

[Phalloidine intoxication. Results of various forms of treatment in 47 patients].

The effectiveness of three plasma cleansing techniques (exchange transfusion, peritoneal dialysis and plasmapheresis) is studied in the treatment of phalloides intoxication. The severity of the latter is dependent upon the amount of toxin ingested, with can now be measured by radio-immunology, as well as the fixation of the toxin in the liver. Methods to ensure its elimination must be instituted as soon as possible. Thus in 43 patients who had consumed more than 50 g of fresh fungi, there were no deaths amongst those treated during the first 36 hours and 7 deaths out of 22 patients treated late. It is not yet possible to define the respective values of each method.

Adolescent↗
PubMed2025

CRISPR/Cas9-directed disruption of wc-2 leads to the absence of fruiting body development in Pleurotus ostreatus.

Light, particularly blue light, is a key environmental factor that induces fruiting in certain agaricomycetes. In this study, we characterized mutant strains of Pleurotus ostreatus with disrupted wc-2, which encodes one of the white-collar proteins, Wc-2, to investigate the role of light in fruiting in P. ostreatus. We introduced two different plasmids containing expression cassettes for Cas9 and two different gRNAs targeting wc-2 separately into the dikaryotic P. ostreatus strain PC9×#64. Among the 11 dikaryotic hygromycin-resistant transformants, six strains did not form fruiting bodies. Genomic PCR followed by sequencing analysis suggested that all six fruitless strains were dikaryotic wc-2 disruptants. Small aggregate structures were not observed in the dikaryotic wc-2 disruptants grown under light conditions, as in PC9×#64 grown in a red box. These results suggest that Wc-2 is essential for the initiation of blue light-induced fruiting in P. ostreatus.

Pleurotus↗
PubMed1975

Production of ethylene and other volatiles and changes in cellulase and laccase activities during the life cycle of the cultivated mushroom, Agaricus bisporus.

Nine volatile hydrocarbons, as well as methyl chloride, carbonyl sulphide and carbon disulphide, have been identified by mass spectrometry as products of Agaricus bisporus in the compost used in comerical mushroom beds. Of these, only ethylene showed a pattern of production that could be correlated with developmental phases of the crop, high levels being produced whenever fruit bodies were rapidly enlarging. In laboratory flask cultures, under controlled conditions, high levels of ethylene occurred whenever young fruit bodies entered the expansion phase. The enhanced rate of ethylene production continued over several days, irrespective of whether fruit bodies were removed. Production occurred within the colonized compost; no ethylene was evolved by the fruit body itself. When the first fruit bodies expanded, either in beds or culture flasks, laccase levels in the compost fell and those of a beta-1,4-glucanase (cellulase) rose. The enzyme switch occurred once only, during maturation of the first fruit bodies, whereas an elevated ethylene production was associated with each occasion when fruit body maturation took place. The low level of laccase and high of cellulase characterized the whole of the reproductive stage of A. bisporus, whereas the phasic periods of high ethylene production distinguished between periods of fruit body maturation and intervening resting periods.

Agaricales↗
PubMed1978

[Cadmium in mushrooms (author's transl)].

The cadmium content of 402 species of wild mushrooms was determined by flameless atomic absorption spectroscopy. Altogether 1049 samples, grown mainly, in southern Germany, were tested. The cadmium content was between less than 0,1 and 120 mg/kg dry weight, equivalent to less than 0,01 and 10,8 mg/kg fresh weight. Samples with low cadmium content were predominant: 68% of the samples contained less than 2 mg/kg dry weight, 86.5% contained less than 5 mg/kg dry weight, equivalent to about less than 0,2 and less than 0,5 mg/kg fresh weight, respectively. The cadmium content was clearly species-dependent, and to a lesser extent genus-dependent. Samples containing more than 10 mg/kg dry weight occurred in 41 species, among these were 9 Tricholomataceae, 10 Agaricaceae, 11 Cortinariaceae, 3 Amanitas and 4 Russula species. Samples containing more than 50 mg/kg dry weight were found in Agaricus augustus, A perrarus, A. silvicola, A. macrosporus, A. maleolens, and Inocybe bongardii. In single fruiting-bodies the lowest cadmium content was found in the stem, whereas the highest content was found in the gills and tubes. Cadmium content of the gills was at most five times the amount present in the cup. In cadmium-rich mushrooms a marked concentration as compared with the cadmium content of the soil had occured.

Basidiomycota↗
PubMed1977

Digoxin elimination by exchange transfusion.

The report covers four cases presenting simultaneous indications for digitalisation and exchange transfusions. Intravenous administration of digoxin was followed: 1. by monitoring of the behaviour of the plasma digoxin level; 2. by determination of the total amount of glycoside eliminated by the blood exchange. Particular attention was paid to the effect of the delay between injection and exchange transfusion on the amount of digoxin eliminated. All four cases showed moderate falls in plasma levels. The amounts of digoxin eliminated by exchange transfusion were in reverse relationship to the delay between administration of digoxin and the blood exchange. At no time did the eliminated fraction exceed 5% of the total amount present in the body.

Child, Preschool↗
PubMed1976

Metabolic mapping of the primary visual system of the monkey by means of the autoradiographic [14C]deoxyglucose technique.

An autoradiographic technique that employs 2-[14-C]deoxyglucose to measure the local rates of glucose utilization within the brain has been applied to the binocular visual system of the Macaque monkey. This method, which pictorially displays the relative rates of glucose consumption in the component structures of the brain, delineates the regions of altered functional activity because of the close relationship between functional activity and energy metabolism. Bilateral retinal stimulation results in the delineation of different rates of glucose consumption in at least four cytoarchitectural layers of the striate cortex. The most intense metabolic activity appears to be in Layer IV, the locus of the termination of the geniculocortical pathway. Bilateral visual occlusion lowers the rates of glucoes consumption in striate cortex and markedly reduces the metabolic differentiation of the various layers. Unilateral visual deprivation delineates the laminae of the lateral geniculate body and the ocular dominance columns of the striate cortex. It also results in the autoradiographic visualization of regions with normally monocular input in the striate cortex, such as the rostral portions of the mushroom-like configurations in the calcarine cortex, which represent the extreme temporal crescents of the visual fields, and small regions in the most caudal part of the mushroom configurations, which are believed to represent the cortical loci of the blind spotsof the visual fields.

Animals↗