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At least 19 recordsLinked to original sources
PubMed1988

Health and living arrangements among older women: does living alone increase the risk of illness?

This study compares the physical, mental, and functional health of aged women living alone to those living with a husband and to those living with others, and considers whether living arrangements lead to health problems or if health problems influence the choice of living arrangements. Data were collected in 1984 during the first wave of a prospective study of 807 women 65 years or older living in an area consisting of 20 contiguous census tracts in Baltimore, MD. Multivariate analyses demonstrate no particular disadvantage associated with living alone for this group of elderly women. After controlling for age and socioeconomic status, analyses indicate that it is those living with persons other than their husbands who are most impaired. In addition, poor health does not appear to result from living arrangement; rather, those who are sickest choose to live with others because of health problems.

Activities of Daily Living↗
PubMed1979

Cellular interaction between fixed and living cells. Transfer of radioactive materials from living cells to fixed cells.

Transfer of radioactive materials to fixed cells from an overlying layer of living cells has been examined to determine whether fixed cells can act as acceptors of glycosyltransferases of living cells. After the incubation of living cells were removed by EDTA treatment, and the radioactivity associated with the fixed cells was determined. Lipids, proteins and carbohydrates were found to be transfered from the living cells to the fixed cells. The amount of radioactivity transferred to the fixed cells was dependent on the number of both fixed and living cells and increased with the time of incubation. When fixed cells were treated with chloroform-methanol before the addition of living cells, the transfer of both lipids and proteins to the fixed cells decreased drastically, but only a slight decrease incarbohydrate transfer was observed. Most of the radioactive materials transferred from living cells labeled with glucosamine or fucose to chloroform-methanol-treated fixed cells were solubilized by trypsin but not by the detergents tested. Approximately 55% of the materials transferred from the cells labeled with glucosamine could be solubilized by hyaluronidase and chondroitinase, and the rest was solubilized by neuraminidase and a glycosidase mixture. The treatment of chloroform-methanol-extracted fixed cells with trypsin caused a significant decrease in the transfer from cells labeled with glucosamine. When nucleotide sugars were used as the radioactive precursor, no significant amount of radioactivity was transferred to the fixed cells.

Autoradiography↗
PubMed1992

The effect of live weight gain and live weight loss on body composition of Merino wethers: dissected muscle, fat, and bone.

Sheep were slaughtered after live weight gain (LWG) and live weight loss (LWL) to evaluate the effect of different periods of normal and retarded growth on dissected carcass components. Thirty-five Merino wether sheep were allowed ad libitum access to the experimental diet of 80% alfalfa chaff and 20% cereal grain (17.23% CP and 18.10 MJ/kg of GE) to grow from 23.0 to 33.0 kg live weight and then fed to lose 10 kg at 133 g/d. Five animals were slaughtered at live weights of 23.0, 26.3, 29.6, and 33.0 kg during LWG and 29.6, 26.3, and 23.0 kg during LWL. Fleece-free empty BW was similar in both LWG and LWL animals at all common slaughter weights because of the lesser and greater weights of the alimentary tract contents and the fleece, respectively, in the LWL animals. Carcass weight or dissected side weight was greater in LWL animals at each common slaughter weight but significantly so (P less than .05) at the 23.0 kg live weight only. Total side muscle weight was similar in LWG and LWL animals at each common slaughter weight, but the proportion of muscle in the dissected side weight was lower (P less than .05) in LWL animals at 23.0 kg live weight. Total side fat, subcutaneous fat, intermuscular fat, and kidney and channel fat weights were all greater in LWL animals at each common slaughter weight, but significantly so (P less than .05) at the 23.0 kg live weight only. Total side bone weight was higher at both 26.3 kg (P less than .01) and 23.0 kg (P less than .05) in the LWL animals. Weight loss in young sheep mobilized more weight of muscle than fat from the carcass, whereas bone weight was constant.

Adipose Tissue↗
PubMed1989

Vanadate inhibits degradation of short-lived, but not of long-lived, proteins in L-132 human cells.

Vanadate, at concentrations higher than 0.04 mM, inhibits the intracellular degradation of short-lived proteins in exponentially growing L-132 human cells. The inhibition is not due to a decrease in viability or in the ATP contents of the cells. Since vanadate decreases proteolysis in cell extracts, the inhibition appears to affect the proteinases which degrade these proteins. Under optimal nutritional conditions, the degradation of long-lived proteins is accelerated by vanadate, thus providing additional evidence that in exponentially growing cultured cells degradation of short- and long-lived proteins occurs by different processes. Vanadate also efficiently inhibits the lysosomal degradation of endocytosed proteins and of long-lived proteins under step-down conditions. However, this effect seems to be unrelated to the observed inhibition of degradation of short-lived proteins, because chloroquine and leupeptin, which inhibit degradation of proteins by lysosomes, do not modify the degradation of these proteins. Our results provide for the first time a probe which, owing to its opposite effects on the degradation of short- and long-lived proteins, could be useful to clarify the mechanisms involved in protein degradation in cultured cells.

Cell Division↗
PubMed1992

The effect of live weight gain and live weight loss on body composition of merino wethers: chemical composition of the dissected components.

Chemical composition of the dissected side parts and dissected side was measured during live weight gain (LWG) and live weight loss (LWL) to determine effects on the chemical composition of the dissected side parts. Thirty-five Merino wethers had ad libitum access to the experimental diet (17.23% CP and 12.09 MJ/kg of DE) to grow from 23.0 to 33.0 kg live weight and then were fed to lose a total of 10 kg in three periods of 25 d each at the rate of 133 g/d. Groups of five animals were slaughtered at live weights of 23.0, 26.3, 29.6, and 33.0 kg during LWG and 29.6 kg (first period), 26.3 kg (second period), and 23.0 kg (third period) during LWL. The greater dissected side weight in LWL animals than in LWG animals at 23.0 kg of live weight was due to the significantly greater chemical fat (P < .05) in the LWL animals than in the LWG animals at 23.0 kg. There were no significant differences between treatments in the protein and water weights in the dissected side. The general increase in the chemical fat in the dissected side of the LWL animals was due to the significant increase in the chemical fat in the muscle (P < .01) and bone (P < .01, 23.0 and 26.3 kg and P < .05, 29.6 kg) at each common slaughter weight and subcutaneous fat (P < .05), intermuscular fat (P < .05) kidney and channel fat (P < .05), and total side fat (P < .01) at 23.0 kg.(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗
PubMed1989

Prevalence, treatment, and outcome of heart disease in live-born children: a prospective analysis of 91,823 live-born children.

All 91,823 children born in 1980 in Bohemia (population 6.314 million; area 52,478 square kilometers) were examined at least four times during infancy and at the age of three and four years. All children who died were autopsied and those with heart disease were selected. A total of 779 children (8.223/1000 live births) were suspected by provincial pediatric cardiologists of having a heart disease. All of these were examined at the age of four years at our Center of Pediatric Cardiology. At this age heart disease was proved in 613 alive or deceased children (6.676/1000 live births), congenital cardiac malformations in 589 (6.415/1000 live births), and cardiomyopathies in 24. The most frequent congenital heart defects (CHD) were ventricular septal defect (VSD) (31.41%), atrial septal defect (ASD) (11.37%), aortic stenosis (AS) (7.64%), pulmonary stenosis (PS) (7.13%), coarctation of the aorta (CoA) (5.77%), and transposition of the great arteries (TGA) (5.43%), followed by persistent ductus arteriosus (PDA) (4.75%), atrioventricular septal defect (AVSD) and hypoplastic left heart syndrome (HLHS) (4.07% each), tetralogy of Fallot (TF) (3.56%), and pulmonary atresia (PA) (2.38%). A prevalence of less than 0.1/1000 live births was found for the remaining cardiovascular defects. One hundred fifty-nine (25.9%) patients were admitted to our highly specialized center, 116 (19.7%) catheterized and 85 (13.9%) treated surgically, during the first four years of life. A total of 440 (71.8%) patients survived the fourth year of life. The percentage of deaths was 25.6% among those with congenital heart diseases and 71% with cardiomyopathies. The overall mortality rate was 27% in surgically and 26% in medically treated patients.

Aortic Coarctation↗
PubMed1985

An improved fluorochrome microassay for the detection of living and non-living intracellular bacteria in human neutrophils.

Acridine orange fluorescence may be used to distinguish living from non-living intracellular bacteria in individual glass-adherent neutrophil granulocytes (PMN). An improvement of the original assay (Smith and Rommel, 1977; Pantazis and Kniker, 1979) is described which allows differentiation between ingested and cell-adherent bacteria. It is shown that this differentiation is impossible with the original method using wet-mounted preparations. With the improved method, however, using dry-mounted preparations, cell-adherent as well as extracellular bacteria lose their fluorescence. Moreover, the fluorescence of cell nuclei and granula is reduced to a minimum. Phagocytosis kinetics and selective inhibition of the myeloperoxidase of PMN show that living intracellular bacteria fluoresce green and non-living bacteria red in such dry-mounted preparations. The preparations can be stored and interpreted for at least 2 months. Application of this method requires 0.1 ml blood or cell-rich body fluid per preparation and is fast and inexpensive.

Acridine Orange↗
PubMedDate not supplied

Proliferative potential and DNA repair in lymphocytes from short-lived and long-lived strains of mice, relation to aging.

The replicative capacity and DNA repair ability of spleen lymphocytes of young and old mice from short-lived and long-lived strains were studied. DNA repair after both UV- and gamma-induced damage was investigated. Proliferation after Con A decreased as a function of age in both mouse strains and paralleled an age-associated decline in repair of DNA damage induced by either UV or gamma-irradiation. Compared to the long-lived, the short-lived strain displayed an earlier impairment of both proliferative and repair potentials. DNA repair after gamma-induced damage only occurred if lymphocytes were stimulated to proliferate. Resting lymphocytes appeared unable to repair strand breaks. By contrast, DNA repair of UV-induced damage showed two components: one was dependent on the cell proliferative state, the other was not. Both components were stimulated or induced by mitogen. Resting lymphocytes were able to perform an appreciable amount of repair after UV irradiation. Our results suggest that resting or post-mitotic cells possess a greater possibility to regulate repair of UV-induced than gamma-induced damage. We speculate that it may be the level of this proliferation-independent, but mitogen inducible form of repair which correlates with maximum life-spans between species, thereby explaining why repair of UV- but not gamma-induced damage reveals such a correlation.

Aging↗
PubMed1978

The replicative life spans of euploid hybrids derived from short-lived and long-lived human skin fibroblast cultures.

Two recent technical advances facilitate the derivation of proliferating hybrids from human diploid fibroblast strains without recourse to biochemical selection: (1) a new chemically-mediated method of somatic cell fusion (PEG-DMSO) yields hybrids at rates as high as 1 in 160 colonies after dilute plating of treated cell mixtures, and (2) a simple technology for assessment of DNA content (flow microfluorometry) permits rapid and highly sensitive monitoring of ploidy. Employing these techniques, we isolated 43 chromosomally stable hybrid clones from 12 crosses between seven different strains representing a wide range of longevities. Crosses between short-lived strains resulted in short-lived hybrid offspring, whereas hybrids derived from long-lived parents tended to be long-lived. Crosses between strains of contrasting longevities gave clones with intermediate growth potentials relative to the other types of hybrids. The failure to observe complementation (enhanced longevity) of hybrids argues against random recessive single-copy gene mutations as important determinants of clonal senescence.

Cell Fusion↗
PubMed1986

Distinct short-lived and long-lived antibody-producing cell populations.

This report analyzes the life span of Ig-containing cells (IgCC) in different sites of antibody production. The experimental approach was based upon the observations that most IgCC are derived from proliferating precursors while IgCC themselves are mainly nondividing end cells. Rats were given a continuous infusion of [3H] thymidine via an osmotic pump inserted in the peritoneal cavity. At intervals of 1, 3, 5 or 10 days after starting infusions, tissues were taken and analyzed by a combination of immunohistology and autoradiography to identify the proportions of IgCC which had gone through S phase of the cell cycle during the period of infusion. After 3 days infusion the median and (range) percent-labeled IgCC in the medullary cords of mesenteric and cervical lymph nodes and the red pulp of the spleen were, respectively, 88 (81-90), 75 (66-77) and 88 (82-93). Conversely that for IgCC in bone marrow was only 13 (11-17) and that in the lamina propria of the jejunum 47 (33-68). The rate of increase in labeling of bone marrow IgCC with length of infusion was approximately linear. Extrapolation of this slope suggests that bone marrow IgCC have a life span in excess of 3 weeks. The slopes of increase in IgCC labeled with time for lymph nodes and spleen were clearly biphasic suggesting that while most IgCC in these tissues have a life span of less than 3 days, there is also a minor population of long-lived IgCC. The lamina propria appears to have approximately equal proportions of long and short-lived IgCC. The life span of IgCC, with the exception of IgMCC, appears to be a feature of the site of antibody production rather than the Ig class produced. Almost all IgM-containing cells were found to be short lived.

Animals↗
PubMed1989

Elderly persons living alone: the effect of community context on living arrangements.

Current explanations of elderly household composition focus largely upon individual demographic, economic, and health characteristics and frequently ignore the effects of the community context within which elderly persons live. In this study, we incorporate the community context into an aggregate explanation of intermetropolitan variation in rates of living alone among elderly persons. We contend that three crucial community resources influence the rate of living alone: demographic and normative environment, economic affordability, and community social services. Data for Metropolitan Statistical Areas in the United States in 1980 provide initial support for this community-level explanation of elderly household composition.

Age Factors↗
PubMed1991

The meaning of living with AIDS: a study using Parse's theory of man-living-health.

This paper explores the meaning of living with Acquired Immune Deficiency Syndrome (AIDS) using Parse's theory of man-living-health as the conceptual framework. The design of the study is based on the qualitative descriptive method. Analysis of the data gathered from interviews with fourteen subjects reveals the emergence of three themes related to the meaning of living with AIDS: (a) an abrupt shift in patterns of being give rise to changing priorities, (b) fluctuating possibilities arise in the uncertainty of being with and away from close others, and (c) changing hopes and dreams surface from the insights of suffering. Practice propositions were identified from the findings of the study.

Acquired Immunodeficiency Syndrome↗
PubMed1991

Alienation of elderly Korean American immigrants as related to place of residence, gender, age, years of education, time in the U.S., living with or without children, and living with or without a spouse.

Dean's Alienation Scale (DAS) was administered to 137 Korean immigrants, aged sixty years or older, who had resided in the United States for at least one year but not more than fifteen years. The study was made in order to determine the relationship of alienation to the independent variables of place of residence (an ethnically homogeneous Korean community in Los Angeles vs. ethnically heterogeneous communities in Oklahoma), gender, age, years of education, time in the United States, living with or without children, and living with or without a spouse. Significant relationships (p less than .01) were found between one or more of the subscales of the DAS (powerlessness, normlessness, social isolation, and total alienation) and place of residence, age, time in the United States, and whether living with or without a spouse. Simultaneous regression analyses were performed to determine the relative contribution of the independent variables to each of the subscales.

Acculturation↗
PubMedDate not supplied

[Lead concentrations in breast milk of women living in urban areas compared with women living in rural areas].

In the last twenty years a great interest has grown up about the accumulation of lead in the environment, because many studies have found relationship between exposure to low lead doses and neuropsychological impairment of children. The Authors have measured and then compared the lead content of milk from women living in city-areas and women living in rural areas, not having professional lead exposure. 34 samples of women milk from the rural areas and 20 samples from the city areas were examined: the lead mean concentration of the rural areas milk samples was 45.62 microgr/l with a range from 0 to 425 microgr/l; the mean concentration of the city areas samples was 126.55 microgr/l with a range from 1 to 472 microgr/l. The data were compared with the Student t-test and the difference was statistically significant at p = 0.01. The Authors examine the correlations between environmental lead accumulation and increased lead concentration in woman milk, laying stress on the possible hazard to health and neuropsychological development of children, mostly of those living in urban areas with heavy road traffic and industrial activity.

Female↗
PubMed1987

Vitamin D status of two groups of elderly in Oslo: living in old people's homes and living in own homes.

Vitamin D status of two groups of elderly was determined by measuring serum concentrations of 25-hydroxyvitamin D3 (25-OHD), and dietary and supplemental vitamin D intake. Group A consisted of 35 women (mean age 86 years), and 21 men (mean age 80 years) living in 5 different homes for elderly. Group B consisted of free-living elderly, 24 women (mean age 76 years) and 7 men (mean age 79 years). There was no or little seasonal variation in serum 25-OHD concentrations in either group. Of those who did not use vitamin D supplements, 83% of the women and 77% of the men in group A, and 40% of the women and none of the men in group B had 25-OHD concentrations below 20 ng/ml in the winter serum samples. Of the 49 individuals who used supplements, only 2 had values below 20 ng/ml. In the institutionalized elderly, 14-15 micrograms vitamin D per day as supplement was associated with a mean increase in 25-OHD concentration in winter serum samples of 25 ng/ml. Dietary intakes of vitamin D were low for both groups, especially for the women (less than 3 micrograms). It is concluded that elderly Norwegians, in particular those living in institutions, ought to improve their vitamin D status by increasing their exposure to sunshine and by including more oily fish in their diet. If this is difficult to achieve, vitamin D supplement (10 micrograms/day) should be used.

Aged↗
PubMed1992

[On the daily living and development of infants living in urban and rural regions].

Temporal pattern of daily living behavior and development in infants aged 3-6 yr were compared between urban and rural regions at Fukuoka-prefecture. The present study consisted of questionnaire on behavior of daily living and tests for development in intelligence and motor function. Rural infants got up and went to bed earlier than urban infants. Urban infants with less physical activity were inferior to rural infants in motor skill. It is suggested that manual dexterity and intelligence may be related.

Activities of Daily Living↗
PubMed1991

[Measurement of activities of daily living in patients with dementia living at home: development of a questionnaire].

In clinical practice and for scientific research a method is needed for the assessment of changes in functioning in daily life of dementia patients living at home. As existing methods have their limitations, a questionnaire was developed (the Interview for Deterioration in Daily life in Dementia: IDDD), to be answered by caregivers. The questionnaire concerns self-care and complex activities, that are usually performed by men as well as women. Attention is paid to the difference between lack of initiative and impaired performance itself. Caregivers are interviewed in a structured interview. A study among 30 caregivers of dementia patients living at home, showed the relevance and usefulness of the questions. The questionnaire shows high internal consistency, which may be the result of our structured interview technique.

Activities of Daily Living↗