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Results for “complete genome sequence”

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At least 19 recordsLinked to original sources

Complete genome sequence of Bacillus subtilis strain S-LA1, a potential plant probiotic endophyte from the medicinal plant Leucas aspera.

Bacillus subtilis strain S-LA1 is an endophytic bacterium isolated from Leucas aspera roots that harbors a 4.2 Mbp genome predicted to encode several traits for nutrient acquisition, plant growth promotion, and plant probiotic efficacy. Genomic characterization underscores its potential as a microbial resource supporting sustainable agriculture and crop disease management strategies.

Bacillus

Complete genome sequence of a novel alternavirus infecting Fusarium falciforme.

We present the complete genome sequence of a novel alternavirus, tentatively named "Fusarium falciforme alternavirus 1 (FfAV1)", isolated from Fusarium falciforme. The host, F. falciforme strain Fod375, was isolated from a soil sample in Spain in 2012 and was found to be infected with a virus containing a tetra-segmented double-stranded (ds) RNA genome. The genome segments, designated as dsRNA1 (3529 bp), dsRNA2 (2641 bp), dsRNA3 (2459 bp), and dsRNA4 (1471 bp), each possess a single open reading frame (ORF). The protein predicted from dsRNA1 contains the typical domains of an RNA-dependent RNA polymerase (RdRP) homologous to those of previously reported alternaviruses, while the protein predicted from dsRNA3 shows homology to alternavirus capsid proteins. The proteins encoded by dsRNA2 and dsRNA4 are of unknown function. All predicted proteins exhibited the highest sequence identity with their counterparts in Hebei alternavirus and Marquandomyces marquandii alternavirus 1. Phylogenetic analysis supported the placement of this FfAV1 isolate within the genus Alternavirus. Considering these results, we propose that FfAV1, along with the two closely related unassigned alternaviruses, represents a new species within the genus.

Genome, Viral

The coding-complete genome sequence of Arabidopsis latent virus 1 from Iraq.

Here, we report the coding-complete genome sequence of Arabidopsis latent virus 1 (ArLV1; Comovirus arabidopsis) from Iraq. The virus was identified by metatranscriptomic sequencing of asymptomatic cucumber (Cucumis sativus) leaves harboring thrips collected from commercial greenhouses. The bipartite genome comprises RNA1 (5,553 nt) and RNA2 (3,582 nt).

Arabidopsis latent virus 1

Complete genome sequence of Acinetobacter bacterium strain BZX-2 isolated from hybrid sturgeon.

We report the complete genome sequence of Acinetobacter sp. strain BZX-2, isolated from a hybrid sturgeon (Acipenser baerii ♀ × Acipenser schrenckii ♂). The genome consists of a 3,819,278-bp chromosome with 3,566 predicted protein-coding genes. The genomic characteristics and antibiotic resistance genes identified provide a basis for the prevention and control of sturgeon diseases.

Acinetobacter

The first complete genome sequence of Ammi majus latent virus from the new natural host culantro.

A potyvirus (isolate AMLV-CQ) infecting culantro (Eryngium foetidum L.) imported from Vietnam was identified by RT-PCR. The complete genome sequence of AMLV-CQ was determined to be 9,549 nucleotides in length. It contains a large open reading frame encoding a 3,082-amino-acid putative polyprotein, flanked by 5´ and 3´ untranslated regions (UTRs) of 77 and 226 nt, respectively. AMLV-CQ is closely related to five other completely sequenced potyviruses, sharing 68-69% nucleotide and 69-70% amino acid sequence identity. However, the coat protein (CP) gene shares 89% nucleotide and 93% amino acid sequence identity with that of a partially sequenced potyvirus, Ammi majus latent virus (isolate AMLV-WF17). These results suggest that AMLV-CQ and AMLV-WF17 are isolates of the same species. To our knowledge, this is the first report of a complete genome sequence of an AMLV isolate, and culantro was identified as a new natural host for this virus. In addition, a one-step RT-PCR assay was developed that provides a rapid, robust, and highly sensitive approach for the detection of AMLV.

Eryngium

Complete genome sequences of three effective nitrogen-fixing strains of Bradyrhizobium ottawaense from Canada.

We report complete genome sequences of three nitrogen-fixing Bradyrhizobium ottawaense strains isolated from soybeans in Canada. Each ~9.0 Mb genome (chromosome and plasmid) harbors predicted genes for nodulation, nitrogen fixation, N2O mitigation, phosphate solubilization, iron acquisition, phytohormone production, and stress tolerance, highlighting their potential for sustainable agriculture.

Bradyrhizobium ottawaense

Complete genome sequences of three co-occurring Pseudomonas isolates from California Botanic Garden topsoil.

We report the complete genome sequences of three Pseudomonas isolates recovered from topsoil at the California Botanic Garden. Two isolates share ~99.4% average nucleotide identity, enabling investigation of intraspecies microvariation; the third represents a co-occurring distinct species, together capturing species- and strain-level genomic diversity within a natural soil community.

Pseudomonas

Complete genome sequence of Streptomyces californicus ADR1, an anti-infective, anti-biofilm and anti-oxidant producing endophyte isolated from the medicinal plant Datura metel.

OBJECTIVE: Streptomyces californicus strain ADR1 is an endophytic actinobacterium isolated from Datura metel that produces secondary metabolites with potent antibacterial and anti-biofilm activities against WHO-listed high-priority Gram-positive pathogens. While anti-bacterial and antioxidant potential of the strain ADR1 has been extensively characterized, its complete genome sequence remains to be investigated for further insights into its biosynthetic potential. This study presents the complete genome sequence analysis of the strain ADR1 to provide a robust genomic foundation for understanding its metabolic versatility and biosynthesis of compounds with therapeutic significance. DATA DESCRIPTION: The ADR1 genome was sequenced using Illumina HiSeq. The assembly comprised 262 scaffolds with a total genome size of 8.4 Mb and G + C content of 72.5%, containing 7427 protein-coding genes. AntiSMASH and IIT-Hyderabad novelBGC analysis revealed 39 biosynthetic gene clusters, including non-ribosomal peptide synthetases, type I polyketide synthases, terpene and melanin clusters, correlating with the diverse therapeutic compounds previously identified through GC-MS analysis. This high-quality genome provides crucial insights into the biosynthetic potential underlying potent antimicrobial and antioxidant activities of the strain ADR1.

Streptomyces