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Results for “bacterial strain isolation”

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At least 19 recordsLinked to original sources
PubMed1979

[Bactericidal activity "in vitro" of sulfamethoxazol-tri-methoprime (SMZ-TMP) alone and in combination with various antibiotics on bacterial strains isolated from urinary tract infections (author's transl)].

The authors study "in vitro" effects of sulfamethoxazol-trimethroprime (SMZ-TMP) on 1 078 bacterial strains isolated from urinary-tract infections during january 1974, the second trimester 1974 and january 1975. Study involves two parts : comparison between bacteriostatic activity of SMZ-TMP, ampicillin and nalidixic acid; evaluation of bactericidal activity of antibiotic associations including SMZ-TMP. MIC study included all strains. On Gram- negative bacilli the bacteriostatic activity of SMZ-TMP (74,6 percent of sensitive strains) was comparable with nalidixic acid (79,6 percent of sensitive strains) and better than ampicillin 31,4 percent of sensitive strains). On staphylococcus strains the bacteriostatic activity of SMZ-TMP (84,6 percent of sensitive strains) is better than ampicillin (36,4 percent of sensitive strains); on streptococcus strains the bacteriostatic activity of ampicillin (71 percent of sensitive strains) is better than SMZ-TMP (5l percent of sensitive strains). 473 associations including SMZ-TMP were studied upon 44 strains of Gram- negative bacilli by bactericidal test. ("Cross disposition" method, derive from "cellophane transfer method".

Ampicillin↗
PubMed2026

Complete genomes of 22 bacterial strains isolated from polluted soil microbiota via enrichment on PFAS as a sole carbon source.

A total of 22 bacterial strains were isolated from PFAS-contaminated soil (Veneto, Italy), after a 5-month enrichment using perfluorooctanoic acid and heptafluorobutyric acid. Whole genomes were sequenced and screened with a curated database of dehalogenase-related proteins. All genomes showed potential for fluorinated compound transformation.

Veneto region, Italy↗
PubMed2026

Genome sequences of four bacterial strains isolated from the phyllosphere of Mangifera indica trees in the polluted tropical city of Medellín, Colombia.

Complete and draft genome sequences of four phyllosphere-associated bacterial strains (Microbacterium radiodurans, Brachybacterium rhamnosum, Sphingomonas citri, and Curtobacterium sp.) isolated from Mangifera indica leaves in polluted Medellín, Colombia, are presented. These resources enable future studies on plant-microbe interactions and phyllosphere microbial mediation of atmospheric pollutants under urban stress.

Mangifera indica↗
PubMedDate not supplied

[The sulfamethoxazole-trimethoprim combination 1st survey into its in vitro activity in brazzaville, Congo Results of 780 bacterial strains isolated at the National Laboratory and the General Hospital in a single year].

The combination of SMZ-TMP has recently been introduced in Congo. This year (1978), it is very used by many Physicians. To help the congolese Physicians working without antibiogram frequently, we have tested 780 strains from August 1977 to August 78. The susceptibility of these strains was: for E. coli 90%, Klebsiella 69,20%, Staphylococcus 93% and for all strains studied Gram + and Gram - the percentage was 75,51. As many authors have showed, the strains of Pseudomonas, Streptococcus faecalis and Providencia are resistant. Our results remain similar to the results obtained by some french and african authors from 1970 to 1975.

Drug Combinations↗
PubMed1979

Role of plasmids in mercury transformation by bacteria isolated from the aquatic environment.

Eight mercury-resistant bacterial strains isolated from the Chesapeake Bay and one strain isolated from the Cayman Trench were examined for ability to volatilize mercury. Mercury volatilization was found to be variable in the strains tested. In addition, plasmids were detected in all strains. After curing, two of the bacterial strains lost mercury resistance, indicating that volatilization is plasmid mediated in these strains. Only two cultures demonstrated ability to methylate mercuric chloride under either aerobic or anaerobic conditions. Methylation of mercury, compared with volatilization, appears to be mediated by a separate genetic system in these bacteria. It is concluded that mercury volatilization in the estuarine environment can be mediated by genes carried on plasmids.

Anti-Bacterial Agents↗
PubMed1977

Rapid identification and antibiotic sensitivity testing of bacteria isolated from clinical infections.

A total of 834 bacterial strains isolated from urine were subjected to rapid biochemical and serological identification and rapid antimicrobial sensitivity testing using Autobac 1. For enterobacteria (742 strains) six tests (acetoin-, beta-galactosidase-, hydrogensulphide-, indole-, ornithin-decarboxylase-and urease-production) correctly identified to genus or species level more than 99% of the strains within four hours. Staphylococci and streptococci (92 strains) were identified with full accuracy within two hours using a rapid deoxyribonuclease assay and immunoelectroosmophoresis and coagglutination. The overall accuracy for the automated antibiotic susceptibility testing using Autobac was 93% as compared to the standard plate diffusion method. In terms of rapidity for 91% of the bacterial strains the susceptibility testing was completed within four hours. After five hours 99% of the strains were analysed. Our data indicate that rapid and automated assays can be accurate and furnish the physician with adequate data within 24 hours after receipt of a urinary specimen.

Agglutination Tests↗
PubMedDate not supplied

[Epidemiological aspects relating to the determination of fosfomycin sensitivity: results of a study on 300 strains isolated in a hospital milieu in Turin].

The AA. evaluated the sensitivity to Fosfomycin and to other commonly used antibiotics of 105 bacterial strains isolated from hospital environment and 195 from pathological materials. They also studied the problem of bacterial resistence to Fosfomycin considering the frequency of "inner colonies" and of bacterial cross-resistance to other antibiotics.

Anti-Bacterial Agents↗
PubMed1979

Histamine production by Klebsiella pneumoniae and an incident of scombroid fish poisoning.

A histamine-producing strain of Klebsiella pneumoniae was isolated from a sample of tuna sashimi implicated in an outbreak of scombroid fish poisoning. None of the other nine gram-negative bacterial strains isolated from the tuna sashimi was capable of equivalent histamine production. Bacterial histamine production was monitored in a tuna fish infusion broth (TFIB), and the implicated K. pneumoniae was capable of producing 442 mg of histamine per 100 g of tuna in TFIB in 7 h under controlled incubation conditions. Only 12 of 50 other K. pneumoniae strains, representing 5 distinct biochemical types, which had been originally isolated from foods, were able to produce such levels of histamine in TFIB. No correlation was found between histamine production and other biochemical characteristics or antibiotic resistance. Of the 12 histamine-producing strains, 11 belonged to type 2, which is characterized as indole negative with positive reactions in the urea and Voges-Proskauer tests. However, only 50% of the type 2 strains examined produced high levels of histamine in TFIB. Additionally, the implicated K. pneumoniae strain and one other strain belonged to type 1, which is characterized by positive reactions in the indole, urea, and Voges-Proskauer tests.

Animals↗
PubMed1979

Subgingival microflora of periodontal patients on tetracycline therapy.

Samples of subgingival plaque were collected from periodontal patients receiving two different tetracycline treatment regimens following conventional periodontal therapy. Four patients had received an oral dose of 1000 mg/day for 2 weeks and nine had received 1000 mg/day for 1 week followed by 250 mg/day for extended time periods. The latter regimen is similar so that commonly prescribed for the chronic skin disease, acne vulgaris. Taxonomic characterization of plaque isolates indicated that the predominant organisms cultured from both treatment groups were species of Streptococcus and the branching, filamentous Gram-positive rods, Actinomyces and Rothia. A much greater microbial complexity was observed among the flora isolated from patients taking 250 mg/day. The latter group harbored many of the fastidious Gram-negative organisms presently implicated in the etiology of periodontal disease. All bacterial strains isolated were tested for resistance to tetracycline by replica-plating cultures on an agar medium containing two-fold dilutions of the antibiotic. Streptococcus, Veillonella and Neisseria were the bacterial genera found to be consistently resistant to tetracycline, with minimum inhibitory concentrations as high as 128 micrograms/ml. Among the Actinomyces, selection for antibiotic resistance was common only within a single species, A. odontolyticus. However, A viscosus strains isolated at 54% of the subgingival flora from one patient did demonstrate an intermediate resistance level, growing on concentrations up to 8 micrograms/ml. Tetracycline resistance was shown by a variety of Gram-negative rods isolated from the low dosage group. Although a minimum number of patients were studied, extensive microbial analyses of the predominating subgingival bacteria indicated that the goal of tetracycline therapy was achieved during the 2-week therapy period at 1000 mg/day.

Actinomyces↗
PubMed1979

Isolation and characterisation of bacteria from abscesses in the subcutis of cats.

Thirty-six closed abcesses in the subcutis of cats were examined. Of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. Twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes only. Bacteriodes was the genus most commonly isolated (28.6% of all isolates) followed by Fusobacterium (19.0%) and Pasteurella (multocida) (13.1%). Peptostreptococcus anaerobius was the most commonly isolated anaerobic species (13.2% of anaerobic isolates and 9.5% of all isolates)and Past, multocida was the most commonly isolated facultative anaerobe (46.8%; 13.1%of all isolates).

Abscess↗
PubMed1979

["In vitro" activity of cefamandole, cefalotin, cefoxitin and cefuroxim against enterobacteria and Staphylococcus aureus isolated in hospital (author's transl)].

The in vitro activity of cefamandole was compared with that of cephalotin, cefoxitin, and cefuroxime against 612 bacterial strains isolated in hospital. Cephalotin was more active than cefamandole and the other two cephalosporins against oxacillin-sensitive Staphylococcus aureus strains. In contrast, the activity of cefamandole was superior to that of the other cephalosporins against oxacillin-resistant strains of S. aureus. This improved activity was confirmed by study in media with a high salt content and by population analysis. The activity of cefamandole against cephalotin-sensitive enterobacteria was superior to that of the other cephalosporins. Its activity was comparable to that of cefoxitin and cefuroxime against cephalotin-resistant strains. However, there were marked variations in this activity in relation to the different species. The therapeutic use of these new cephalosporins, and of cefamandole in particular, should thus be selective and based on the results of bacteriologic studies.

Cefamandole↗
PubMed1976

Characteristics of lipopolysaccharides of Salmonella typhi isolated from carriers and patients suffering from typhoid fever.

Lipopolysaccharides (LPS) of Salmonella typhi strains, isolated from carriers and patients suffering from typhoid fever, were characterised according to their biochemical properties, morphological structure and degree of aggregation of complexes. All preparations of LPS, regardless of their origin, were morphologically heterogeneous. Free electrophoresis and immunoelectrophoresis demonstrated that LPS preparations were composed of components possessing different mobilities in electric fields. LPS of bacterial strains isolated from both carriers and patients, split upon reaction in immunoelectrophoresis with specific antiserum 73, rabbit antiserum to Salmonella typhi Vi Bhatnagar and 0-901 split into anodic and cathodic fractions. The anodic fraction reacted similarly as Vi antigen. LPS from Salmonella typhi Ty-2 yielded only the cathodic fraction, typical for O antigen. LPS from strains which were passaged twice in nutritional medium possessed identical properties as LPS from fresh cultures of Salmonella typhi. Electron microscopy revealed that LPS appears as long bands, rods, ellipsoid forms and amorphous material. Contrary to amorphous material, the bands, rods and ellipsoid forms possessed three-layer structure.

Antigens, Bacterial↗
PubMed2025

Functional metaproteomics for enzyme discovery.

Discovery of microbial biocatalysts traditionally relied on activity screening of isolated bacterial strains. However, since most microorganisms cannot be cultivated in the lab, such an approach leaves the majority of the microbial enzyme diversity untapped. Metagenomic approaches, in which the DNA from a microbial community is directly isolated and then used either for the creation of an expression library or for sequencing and metagenome annotation have alleviated this shortcoming to an extent, but have their own limitations: the generation of large expression libraries is time-consuming and their screening is costly, while metagenome annotation can infer biocatalytic function only from prior knowledge. We have thus developed a functional metaproteomic approach, which combines the immediacy of traditional activity screening with the comprehensiveness of a meta-omics approach. Briefly, the whole metaproteome of an environmental sample is separated on a 2-D gel, biocatalytically active proteins are visualized in-gel through zymography, and those candidate biocatalysts are then identified through mass spectrometry, searching against a metagenome-derived database obtained from the very same environmental sample. Here we explain the process in detail, with a focus on esterases, and give guidelines on how to develop a functional metaproteomic workflow for enzyme discovery.

Proteomics↗
PubMed1975

Bacterial blocking activity of specific IgG in chronic Pseudomonas aeruginosa infection.

A factor has been demonstrated in the serum of some patients with chronic Gram-negative infections which specifically blocks bactericidal activity against the infecting organism. Sera with this factor, that had been obtained from patients suffering from chronic Pseudomonas aeruginosa infections, were fractionated by Sephadex G-200 and DEAE-Sephadex column chromatography. Blocking activity was detected in the Sephadex G-200 '7S' peak and eluted from the DEAE column along with the serum IgG. Immunoelectrophoresis studies of this material along with pure IgG showed that the bactericidal blocking factor in these patients was IgG. The blocking factor was specific in its ability to protect bacteria, and could be absorbed from the serum by the particular bacterial strain isolated from the patient. Possible clinical importance of blocking activity by IgG is suggested by the persistent nature of the Ps. aeruginosa infections in these patients.

Antibodies, Bacterial↗