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At least 19 recordsLinked to original sources

Foods predictive of nutrient intake in Chinese diet in Taiwan: II. Vitamin A, vitamin B1, vitamin B2, vitamin C and calcium.

Dietary consumption data obtained by food weighing for 539 households in Taiwan in 1980-1981 were used to search for predictor foods of individual intake of several vitamins and calcium. Stepwise multiple regression analysis was carried out on food items that were univariately and significantly associated with nutrient intakes, to screen for the important predictors. Less than ten foods were enough to explain more than 90% of the interperson variation for vitamin A, vitamin B1, and vitamin B2. For vitamin C and calcium, 20 food items could explain only 87% of the variation. Salt, brown sugar, and soy bean sauce were selected among calcium predictors. The information on foods predictive of nutrient intake is useful in designing food frequency questionnaires and materials for nutrition education. Although this paper provides such information for Chinese living in Taiwan, some issues described (such as the need to assess spices) may have been overlooked and could be generalized to populations using stir-frying as a major cooking method.

Ascorbic Acid↗

[Enzymatic activity of xenobiotic metabolism in rats with a varying body allowance of vitamin B1].

Vitamin B1 in rats induced in the liver elevation of activity of metabolic enzymes of xenobiotics bound with membranes (dimethylaniline N-demethylase, aniline n-hydroxylase, aryl esterase). At the same time activity of the cytoplasmatic enzymes alcohol dehydrogenase and glutathione-S-transferase was appreciably lowered. An additional load with thiamine (20 mg/kg) led to a drop in activity of the membrane-bound enzymes. Vitamin B1 deficiency modified the effect of the inductor phenobarbital. Additional administration of vitamin B1 to thiamine-deficient animals normalized the thiamine level in the liver, and activity of hydroxylase, aryl esterase, formaldehyde dehydrogenase, and significantly decreased demethylase activity. In vitamin B1-deficient animals high detergent concentrations significantly suppressed NADH-dichlorophenol-indophenol-reductase activity, while low concentrations activated this enzyme as compared to the control.

Animals↗

[Vitamin B1].

Vitamin B1 (thiamin), taken-up into cells, is converted to thiamin diphosphate (TDP), and TDP acts as a cofactor for several enzymes involving in carbohydrate metabolism. CoA-dependent oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase multienzyme complex (PDC) with NAD+ as an electron acceptor in most organisms involving mammals and higher plants. PDC consists of three component enzymes, one of which is pyruvate dehydrogenase (lipoamide) which contains TDP as a prosthetic group. Similar multienzyme complex for 2-oxoglutarate or branched chain 2-oxoacids is also found in mammals. In anaerobic bacteria, archaebacteria and anaerobic protozoa, pyruvate:ferredoxin oxidoreductase (PFOR) functions for the oxidative decarboxylation of pyruvate with ferredoxin in place of NAD+. PFOR contains TDP as a cofactor; however its structure is quite different from PDC and 1-3[4Fe-4S] clusters are involved as redox centers. Pyruvate:NADP+ oxidoreductase (PNOR), which catalyzes the oxidative decarboxylation of pyruvate with NADP+ as an electron acceptor, occurs in mitochondria of Euglena gracilis, a protist containing chloroplasts. PNOR consists of two functional domains, one of which contains TDP and 3[4Fe-4S] clusters and resembles PFOR. Another domain involves FMN and FAD as redox centers and its structure is similar to NADPH-cytochrom P450 reductase.

Carboxy-Lyases↗

[Simultaneous determination of vitamin B1 and vitamin C in aqueous solution with piezoelectric crystal quartz sensors].

According to the response properties of piezoelectric crystal quartz sensors to the solution conductivity the frequency shift response was derived to show a linear dependence on the concentrations of vitamin B1 (VB1) and vitamin C (VC). This was experimentally verified and a calibration model for simultaneous determinations of the two vitamins was established. The usefulness of the technique was evaluated by quantitation of mixtures of unknown composition using common multiple linear regression (MLR) and partial least squares (PLS). The average relative standard deviations for six samples were 4.46% for VB1 and 6.63% for VC with MLR; and 1.97% for VB1 and 2.74% for VC with PLS, respectively.

Ascorbic Acid↗

[Effect of vitamin B1 and vitamin E on methionine poisoning in broiler chickens].

An experiment was carried out with 100 broiler birds of the Leghorn breed, aged 20 days. The birds were divided into groups and were given various amounts of methionine--0,5, 2, and 3 per cent with the basic standard mixture. The clinical signs of methionine intoxication were followed up--drowsiness, weakness in the legs, opisthotonus, and twisting of the neck (torticollis) alons with the loss of equilibrium. It was found that with birds receiving 0.5 per cent methionine with the diet the amount of vitamins A, E, and B1 in the blood serum and the liver rose considerably, while with birds given 2 and 3 per cent methionine with the feed the amount of these vitamins dropped essentially. Therepeutic treatment with 3 mg of vitamin E and vitamin B1 each in the course of 4 to 6 days yielded a good effect. An improvement of the general condition and disappearance of the most typical symptoms of methionine intoxication were recorded.

Animal Feed↗

Assessment of thiamin (vitamin B1) and riboflavin (vitamin B2) status in an adult Mediterranean population.

The aim of the present study was to assess the nutritional status for thiamin (vitamin B1) and riboflavin (vitamin B2) in an adult Mediterranean population, in order to identify patterns of intake, groups at risk for deficiency and factors that might influence this risk. A cross-sectional survey was carried out in Andalusia, a western Mediterranean region in southern Spain. Nutrient intakes were studied in a random sample of 3390 subjects (1746 men, 1644 women) who were between 25 and 60 years of age. Blood samples were obtained for biochemical assays in a random subsample of 372 subjects (181 men, 191 women). Food consumption was assessed by 48 h recall. Vitamin B1 and B2 were measured as erythrocyte transketolase and as erythrocyte glutathione reductase activation coefficients, respectively. Energy and vitamin intakes were significantly higher in men than in women. Intakes were below two-thirds of the recommended dietary allowance for vitamin B1 in 7.80 % of the men and 4.50 % of the woman, and were below this level for vitamin B2 in 18.00 % of the men and 11.70 % of the women. Age, educational level, alcohol use and smoking were also associated with differences in the intake of these nutrients. Biochemical analyses showed that vitamin B1 and B2 status was deficient in 6.40 and 5.30 % of the population, respectively. Although factors such as gender, age, level of education, drinking and smoking can have an effect on the risk of inadequate intake of these nutrients, these factors did not affect biochemical indices of nutritional status in the present study.

Adult↗

[Content of free and bound thiamine diphosphate in the liver hyaloplasm of vitamine B1 deficient rats].

The amount of free and protein-bound thiamin diphosphate (TDP) in the liver hyaloplasm of B1 vitamin deficient rats has been measured. In the norm the content of protein-bound TDP remains stable (4.5--4.7 micrograms/g tissue) and does not grow upon thiamin injections. The level of the free coenzyme varies appreciably: in the B1-avitaminotic state the content of free TDP decreases, and in the B1-saturated condition it may exceed the norm 4 times. In the liver this enzyme occurs only as a holoenzyme. In case of B1 vitamin deficiency in the diet the transketolase apoform cannot be detected in the liver. A new model for rapid generation of B1-avitaminosis characterized by a significantly lower level of free and bound TDP is described.

Animals↗