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[Anatomic and histologic studies of a Triatominae subfamily (Heteroptera, Reduviidae). Triatoma infestans' brain and its nerves].

To give a concrete aspect of structure, topography and function of the different nervous systems of the Triatominae, we begin this study with a description of the senso-motor system. As the first part we choose the study of the brain and its nerves. The external form of the brain is conditioned by the anatomy and morphology of the head. The brain is situated in the posterior part of the head beneath and behind the ocelli. It is characterized by the reduction of its longitudinal extension and, by this, the connectives between Syncerebrum and Gnathocerebrum are extremely short, but large, and the third commissure is included in the mass of the mandibular segment of the Gnatocerebrum. The optical masses are of the typical form, also the optical commissure. The Central Corps is volumous and is directly linked with the majority of the centres of the Protocerebrum and Deutocerebrum. The Pedunculated Corps has only one glomerulus that looks like a pedunculated mushroom without any signal of the formation of a calyx, found in ants and other more evoluted insects. Beneath the Central Corps, the extremities of the pedunculus disintegrate and form a number of claviform annexes like in the primitive Machilidae. The Deutocerebrum is characterized by volumous antennal centre, composed by numerous little glomeruli in peripheral situation. The Tritocerebrum is very reduced and has in its anterior part two very short frontal connectives and, by this, the frontal ganglion lies near the Tritocerebrum. The compact mass of the Gnathocerebrum shows its composition by three pairs of ganglions only by observation of series of preparations. With exception of the Lobi optici, 8 pair nerves and 3 impair nerves come from the brain: From the Protocerebrum: the nerves of the ocelli and the nerve of the Corpus allatum. From the Deutocerebrum: the nerves of the antennae and those of the pharynx. From the Tritocerebrum, the nerves of the labrum and, by the frontal ganglion, the Nervus recurrens and the nerve of pharyngeal muscles. From the Gnathocerebrum: the nerves of the amndibulae, maxillae, labium and of the salivary glands.

Animals

A comparison of sampling techniques for domestic populations of Triatominae.

Six methods of sampling domestic populations of Triatoma infestans (Klug in Brazil have been studied. The results suggest that where bug densities are low, the Gomez-Nuñez box may be used, but with higher bug densities withdrawal or removal collecting allows a direct estimation of the size of the bug population. The number of samples to be taken in removal collections depends upon the density of the bug population and the degree of accuracy required.

Humans

Dipetalogaster maximus (Hemiptera, Triatominae) for xenodiagnosis of patients with serologically detectable Trypanosoma cruzi infection.

In patients serologically positive for Trypanosoma cruzi infection the three bug species/instar combinations used in xenodiagnosis showed third-instar Dipetalogaster maximus to be more efficient in detecting circulating trypanosomes than the first instar of the same species which, in turn, is more sensitive than third-instar Triatoma infestans. The sensitivity of the pool technique of faecal examination compared with individual dissection was investigated. Four pool examinations (the product of 20 bugs) were equivalent to 10 individual bug dissections. Because of the ease of providing large numbers of bugs for mass xenodiagnosis, first-instar D. maximus has replaced third-instar T. infestans in our routine work. The value of third-instar D. maximus as a xenodiagnostic agent needs further investigation.

Chagas Disease

Improvements in routine xenodiagnosis with first instar Dipetalogaster maximus (Uhler 1894) (Triatominae).

First instar Dipetalogaster maximus proved as effective as third instar Triatoma infestans in detecting circulating Trypanosoma cruzi in patients with chronic infections examined in a routine xenodiagnostic laboratory. Since rearing costs are diminished, D. maximus has now replaced T. infestans as the xenodiagnostic agent in our laboratory. However, further work is needed to define the optimal conditions necessary when using this species in xenodiagnosis.

Animals

Biochemical characterization of some species of Trypansoma (Schizotrypanum) from bats (Microchiroptera).

Trypanosoma (Schizotryanum) species isolated from bats (Microchirotera) in Europe and Latin America were examined by determining the buoyant densities of their nuclear and kinetoplastic deoxyribonucleic acid (DNA) and the electrophoretic patterns of six isoenzymes. By these criteria they were separated into three distinct groups -- two from Europe (T. dionisii and T. vespertilionis) and one from America. T. dionisii was also separable by its morphology in vitro. Geographical location and DNA buoyant densities suggested that the American stocks were more closely related to T. cruzi than to the European species, though they differed from it marginally in kinetoplastic DNA density and in being noninfective to mice. Similar stocks studied by other workers have been shown to differ from T. cruzi also in reduced infectivity to, and lack of natural association with, Triatominae, and in antigenic composition. It is therefore proposed that trypanosomes of the subgenus Schizotryanum occuring naturally in Microchiroptera and differing from T. cruzi sensu stricto as outlined above should be treated as a distinct subspecies, T. cruzi marinkellei ssp. nov. T. cruzi sensu stricto thus becomes the nominate subspecies T. cruzi cruze Chagas 1909.

Alanine Transaminase