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At least 19 recordsLinked to original sources
PubMed1981

Subcutaneous, isogeneic transplantation of duct-ligated pancreas in streptozotocin-diabetic mice. Relationships between carbohydrate tolerance and hormone content in transplant or host pancreas.

Mice with subcutaneous, isogeneic transplants of duct-ligated pancreas from either two or five donors displayed impaired glucose tolerance to gastric or intravenous glucose, or to an overnight fast followed by a 15-min meal of mouse food. Compared with peripheral insulin responses in normal controls, those of transplant recipients were less after gastric or intravenous glucose, but no different after the meal. Despite normalization of peak insulin levels in the peripheral circulation of isografted mice, glucose clearance was still impaired and this probably resulted, in part, from a relative insufficiency of insulin in the portal circulation. Results of glucose tolerance tests, after transplant recipients consumed a relatively small amount of food, suggested that near-normal glucose homeostasis may be present for these mice under normal feeding conditions. In mice that received incremental doses of streptozotocin, impaired glucose tolerance to a meal was observed if pancreatic insulin content fell below 26% of normal. We failed to show a similar relationship between glucose tolerance and pancreatic insulin content in transplant recipients since all showed impaired glucose tolerance. Despite the initial transplantation of different amounts of islet tissue, at termination insulin content (transplant plus endogenous pancreas) was essentially the same for all recipients, totaling 19-22% of that found in a normal mouse pancreas.

Animals↗
PubMed2001

Role of gonadotropins in malignant progression of sex cord stromal tumors produced by sequential auto- and isogenic transplantation of ovarian tissue in ovariectomized rats.

PURPOSE: In this study the effect of continuous stimulation of gonadotropins on sex cord stromal tumors in the rat was examined. METHODS: Sex cord stromal tumors were induced by transplantation of ovaries under the splenic capsule of ovariectomized rats. Beginning 180 days after transplantation, these tumors were taken out and cut into several pieces, which were then retransplanted (by isotransplantation) under the splenic capsule of 80 either intact or ovariectomized rats. RESULTS: Most of the tumor grafts grew up to a median size of 0.7 cm in ovariectomized rats. However, some of the tumors recovered from recipient rats that were retransplanted with donor tumors differed significantly from the others. Characterized by a high mitotic rate, nuclear atypia, size (up to 3.8 cm) as well as growth in intact animals, these tumors were defined as malignant. They could be kept in culture and always led to the development of metastases after retransplantation into other rats. CONCLUSION: Benign sex cord stromal tumors can show malignant growth after transplantation. This study for the first time demonstrates that gonadotropins are involved in the induction of ovarian malignancies.

Animals↗
PubMed1981

Reversal of diabetes by isogeneic transplantation of cultured pancreatic islets.

Pancreatic islets were isolated by collagenase digestion from female Wistar rats and cultured at 20 mmol/l glucose. The enhancement of Mg++ concentration from 0.8 mmol/l up to 5.3 mmol/l had a protecting effect on the glucose-induced insulin release in the subsequent short-time incubation and prevented the age-depending decrease of B-cell function. About 1,000 cultured islets injected into portal vein normalized the plasma glucose of streptozotocin-diabetic rats. The plasma glucose patterns during the glucose load were nearly identical to healthy controls. These findings suggest that the cultured islets maintain the ability to secrete insulin in response to glucose in vitro as well as in vitro and that such islets can reverse an experimentally induced diabetes.

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PubMed1980

A method for multiple intraarterial injections in the allogeneic or isogeneic transplanted rat kidney by use of a long term catheter in the renal artery.

An experimental model using a permanent catheter in the artery of a transplanted rat kidney is presented. The experimental set-up allows selective infusion or injection into the renal circulation of unrestrained non anesthetized rats. It is also possible to make repeated studies of the renal circulation in a rejecting rat kidney transplant. Preliminary investigations and future applications are discussed.

Animals↗
PubMed1976

Subcutaneous, isogeneic transplantation of duct-ligated pancreas in streptozotocin diabetic mice: relationships between recovery and hormone contents in transplants or host pancreas.

Recovery from diabetes was observed in streptozotocin-treated mice that received subcutaneous, isogeneic transplants of duct-ligated pancreas. Transplants excised from recovered hosts contained both immunoreactive insulin (IRI) and glucagon (IRG), indicating that both A and B cells capable of hormone storage were present. The IRI content in transplants, although only one sixth of that transplanted 6 wk earlier, was still 21/2 times greater than that in the host pancreas and was inversely related to the plasma glucose of the recipient during and after recovery. The IRI content in the transplant added to that in the host pancreas totaled 13% of the IRI found in the normal mouse pancreas, which sufficed for over-all recovery from diabetes but was insufficient to provide normal glucose tolerance and insulin response to a major glucose challenge. The abnormally high content of glucagon noted in the pancreas of hyperglycemic, sham transplanted mice was reduced by one-half in the pancreas of those transplanted mice returning to normal plasma glucose and insulin. Thus, the insulin content of the transplant was important to the recovery of isografted mice, but in addition, and perhaps as a consequence of recovery, there was a slight increase in the insulin storage capacity of the host pancreas and a marked reduction of glucagon compared to the content of these hormones in the pancreas of hyperglycemic, sham transplanted mice.

Animals↗
PubMed1982

The glomerular filtration rate of isogeneically transplanted rat kidneys.

The glomerular filtration rate (GFR) was determined in rats with an isogeneic kidney transplant and compared with that of unilaterally nephrectomized rats. Experiments were carried out in adult rats, 3 months of age, weighing approximately 300 g, as well as in juvenile rats, 6 to 8 weeks of age, weighing approximately 170 g. All donor kidneys were taken from adult rats. The GFR was measured regularly, using a chromium 51-EDTA clearance technique which permitted repeated measurements to be taken in the same animals, during a 15-week followup period. After unilateral nephrectomy the GFR per 100 g body weight (BW) increased compared with that of a single normal kidney. Adult transplant recipients had a GFR per 100 g BW of about 80% of that of unilaterally nephrectomized rats. There was no statistical difference in the GFR when comparing adult recipients of either a normal or a hyperfunctional kidney. When isografts were transplanted to juvenile recipients, there was an initial decrease in the absolute GFR compared with the donor value in the case of a normal adult donor kidney. This decrease was even more pronounced when a hyperfunctioning kidney was transplanted to a juvenile recipient. However, when related to BW the GFR was, as in the adult recipients, about 80% of that of unilaterally nephrectomized juvenile rats. During the followup period the systolic blood pressure was measured regularly by tail plethysmography, in order to detect any blood pressure elevations, which are a frequent complication in adult and pediatric human renal transplantation. However, no hypertension was observed after isogeneic kidney transplantation in the various groups. These results show that the GFR of isogeneically transplanted rat kidneys amounts to about 80% of the maximally attainable level. Isogeneic transplantation of an adult kidney to a juvenile recipient results in a rapid adaptation of the GFR to the smaller size of the body and does not cause an increase in blood pressure.

Age Factors↗
PubMed1982

Morphogenetic potential of rat growth cartilages as isogeneic transplants in the interparietal suture area.

The proximal end of the tibia or the spheno-occipital synchondrosis with some adjoining bone were isogeneically transplanted across the interparietal suture of 10-day-old rats. As a sham procedure, a piece of calvarium traversed by a part of the interparietal suture was interchanged between pairs of animals. Untreated rats served as controls. The animals, injected with Alizarin red S, were killed 25-75 days after the operation, 10 in each group. The transverse dimensions of the neurocranium were larger in the rats with the cartilage transplants than in the controls, particularly at 25 days after the operation; the differences persisted longer in the animals with the synchondroseal transplant. The orientation of the bone interdigitation at the anterior lambdoidal suture changed temporarily in response to the excessive lateral displacement of the parietal bones. The observations indicate that basicranial synchondroses, like epiphyseal cartilage, are endowed with a tissue-separating property that may persist for a prolonged period under transplant conditions. As such cartilaginous structures may also be affected by environmental influences, there must be a two-way interaction between the synchondroses and their immediate environment.

Animals↗
PubMed1976

Subcutaneous, isogeneic transplantation of either duct-ligated pancreas or isolated islets in streptozotocin diabetic mice.

Recovery from hyperglycemia was observed in streptozotocin diabetic mice that received subcutaneous, isogeneic transplants of either isolated islets or duct-ligated pancreas. Transplants of isolated islets obtained from collagenase-digested adult pancreas provided recovery from hyperglycemia, but the incidence of recovery depended on the amount of islet tissue initially transplanted. Hyperplastic, insulin-rich islets obtained from the pancreas of obese hyperglycemic mice (ob/ob) allowed recovery between 3 and 6 weeks, whereas an equivalent number of islets obtained from non-obese, normal donors gave only partial recovery after 8 weeks. Implantation of pancreatic endocrine tissue obtained from adult donors whose pancreatic ducts were ligated several weeks earlier, led to consistent recovery within 8 to 10 weeks. The content of immunoreactive insulin (IRI) extracted from transplants of mice recovering from hyperglycemia was 16 to 19% of that found in the normal mouse pancreas and was about 4 times greater than that remaining in the recepient's own pancreas. Transplants removed from hosts that did not recover contained a relatively small amount of IRI indicating that these transplants contained insufficient insulin stores to allow recovery form hyperglycemia.

Animals↗
PubMed1977

Differences in the osteoinductive potential of transplanted isogeneic dental structures of the rat.

Pulp tissue, enamel and incisal and basal dentine of the mandibular incisor were taken from one litter of rats and transplanted subcutaneously or intracerebrally to sex-matched, 5-day-old animals of the subsequent litter of the same parents. As sham-operations the mere transplantation instrument was inserted into the transplantation sites. With some exceptions, the host animals were killed 4, 32, 128-138 and 210 days after the operation and the transplantation sites were examined either grossly or microscopically or both. The pulp tissue transplantation had resulted in formation of osseous tissue observed 128 days post-operatively. Bone was found in association with many of the basal dentine transplants 128-210 days after their insertion whereas no such tissue was observed with the transplanted enamel of incisal dentine. The sham-operation seemed to have elicited intracerebral bone formation in two animals. The osteoinductive activity of the transplanted tissues seemed reduced by their mineral phase. Further, in contrast to demineralized hard tissues, the non-demineralized inductive agents do not seem to possess bone morphogenetic properties.

Animals↗
PubMed1991

Growth potential of the rat mandibular condyle as an isogeneic transplant traversing the interparietal suture.

The mandibular condyle of 5-, 10- or 20-day inbred male rats was transplanted across the interparietal suture of male litter-mates and the transverse dimension of the neurocranium was measured from dry skulls at 25 or 35 days. The width of the neurocranium had increased significantly from days 5 or 10 to 25 in the rats with the transplants, whereas the difference from controls was small or non-existent by 35 days. Histological examination showed that the cartilaginous zone was reduced in the transplants at 25 days but that there were still layers of chondrocytes at 35 days. It is suggested that the rat mandibular condyle is endowed with a tissue-separating, interstitial growth potential that, to some extent, is unrelated to mobility, and essentially effected by chondrocyte hypertrophy.

Animals↗
PubMed1981

Subcutaneous, isogeneic transplantation of duct-ligated pancreas in streptozotocin diabetic mice II. Hormone storage as a function of time and of the recipient's initial glycemic state.

Between 7 and 18 wk after transplantation, the insulin contained in duct-ligated, pancreas transplants increased twofold whereas glucagon content showed no significant change. Insulin contents of the recipient's own endogenous pancreas, though severely depleted after streptozotocin injection, also showed a twofold increase. The combined insulin reserves in transplant in mice at 18 wk which totaled 30% of that in aged-matched, normal controls was still insufficient to promote normal tolerance and insulin response to intravenously injected glucose. In a second experiment reported herein, we found that the insulin and glucagon reserves in ten week-old transplants were not significantly different between recipients that were either diabetic (streptozotocin-treated) or nondiabetic at the time of transplantation. Thus, the initial glucose levels in the host environment, whether elevated or not, did not differentially effect the level of hormones eventually accumulated in transplants.

Animals↗
PubMed2003

Acute ischemia/reperfusion injury after isogeneic kidney transplantation is mitigated in a rat model of chronic renal failure.

The influence of chronic renal failure on renal susceptibility to an acute ischemic insult was evaluated. Recipient Lewis rats were randomly assigned to undergo 5/6 nephrectomy (chronic renal failure, CRF) or sham operation (normal renal function, NRF). After 11 weeks, normal kidneys of Lewis donor rats were transplanted in the recipients. The outcome of the isografts was assessed. Filtration capacity of the isografts in the CRF rats was preserved to approximately one-quarter of its normal capacity on the 1st day post-transplantation, whereas it fell to 0 in the NRF rats. This was reflected by a significantly higher increase in serum creatinine in the latter group. The isografts in the CRF rats had a significantly lower degree of acute tubular necrosis and no increase in the number of macrophages and T lymphocytes in the first 24 h in contrast to the NRF rats. Epithelial regeneration and repair started earlier in the CRF group. In conclusion, the present study indicated that CRF blunted ischemia/reperfusion injury of a transplanted kidney, and that its regeneration capacity was certainly not hampered by the presence of chronic uremia. These results will be the basis for studies on modulation of early leukocyte-endothelial interactions resulting from immunological disturbances inherent to the uremic environment.

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