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At least 19 recordsLinked to original sources
PubMedDate not supplied

Design of preservation solutions for universal tissue preservation in vivo: demonstration of efficacy in preclinical models of profound hypothermic cardiac arrest.

The design of new solutions for the universal preservation of tissues is a quest that would facilitate multiple-organ harvesting from organ donors since current preservation solutions do not provide optimum preservation for all organs. In contrast, a new approach to bloodless surgery using hypothermic blood substitution (HBS) to protect the whole body during profound hypothermic circulatory arrest (clinical suspended animation) has focused on the development of a hybrid solution design with the objective of providing universal tissue preservation. In this study, a porcine model of uncontrolled lethal hemorrhage was employed. A combination of two new solutions, maintenance and purge, was used in a cardiopulmonary bypass (CPB) technique to affect profound hypothermia and prolonged cardiac arrest (60 min), with resuscitation after surgical repair of the vascular deficit induced to affect exsanguination. After rewarming and recovery, pigs were monitored for 6 weeks for neurological deficits, cognitive function (learning new skills), and organ dysfunction. All the normothermic control animals died (n = 10), whereas 90% (9 of 10) in the HBS group survived (P < .05). Moreover, all of the survivors were neurologically intact, displayed normal learning and memory capability, and had no long-term organ dysfunction. Histology of brains after 6 weeks revealed no ischemic damage in marked contrast to control animals, which all showed diffuse ischemic damage. The demonstrated efficacy of these synthetic, acellular HBS solutions for protection of all the tissues in the body during clinical suspended animation justifies their consideration for multiple-organ harvesting from cadaveric and living donors.

Animals↗
PubMed1995

Reovirus 3 not detected by reverse transcriptase-mediated polymerase chain reaction analysis of preserved tissue from infants with cholestatic liver disease.

Reovirus type 3 has been implicated in the origin and pathogenesis of extrahepatic biliary atresia and idiopathic neonatal hepatitis, but routine detection of this virus in hepatobiliary tissues from affected infants by culture and histological techniques has been unsuccessful. In this study, oligonucleotide primers specific to the M3 genome segment of reovirus 3 (Dearing) were used in a reverse transcriptase-mediated polymerase chain reaction technique to develop a sensitive and specific assay for the detection of reovirus 3 RNA in formalin-fixed, paraffin-embedded patient samples. Optimal reaction conditions were determined by testing infected murine tissues and preserved human liver tissue supplemented with reovirus 3. Archival specimens from 50 infants, including 14 with extrahepatic biliary atresia, 20 with idiopathic neonatal hepatitis, and 16 age-matched controls, were evaluated. Successful amplification of human albumin complementary DNA from the preserved tissues confirmed the presence of intact RNA in every patient specimen tested. Analysis of the amplification reactions by agarose gel electrophoresis and Southern blot hybridization detected the presence of reoviral RNA only once in a single patient sample. These results do not support a strong role for reovirus 3 in the development of neonatal cholestatic liver disease. The recent association of other RNA viruses of the Reoviridae family with murine liver disease and human extrahepatic biliary atresia indicates that continued investigation into a viral cause for idiopathic neonatal hepatobiliary disease is warranted.

Animals↗
PubMed2001

A new animal model for tissue preservation.

In vivo preservation of tissues may exist as a problem in experimental and clinical research. Adipose tissue, nerves, and vessels are the tissues that are usually preserved in vivo for future use or for the evaluation of results in experimental research. Limited volume and difficult conditions in such areas in animal models usually create disappointing results, because of the difficulty in distinguishing the experimental from the surrounding tissues; the insufficiency of the volume of space; and the lack of compliance in animals. A new rat model for in vivo preservation studies is described. A muscular pocket designed between the external and internal oblique muscles is a good choice as an animal model for tissue preservation in plastic surgery research.

Abdominal Muscles↗
PubMed2004

Cell and tissue preservation and storage for transplantation. Present and future.

Historical remarks and data concerning the present status of tissue preservation and storage are presented. Even though, the need for preserved tissue allografts is still very high and tissue bank services are very popular, the opinion is expressed that the future of tissue banking may be correlated with organ and tissue engineering.

Animals↗
PubMed2002

Stability of cytochrome P450 enzymes in human liver samples stored in different tissue preservation buffers.

The effects of various tissue preservation buffers on cytochrome P450 (P450)-mediated activities of microsomes prepared from fresh surgical liver samples were examined. Two individual human liver samples were obtained, and three portions of each were preserved in one of three solutions: phosphate buffer, Krebs-Heinseleit solution, or University of Wisconsin solution. Microsomes were prepared within 24 h, 3 days, and 7 days after the resection of the samples. Marker P450 activities were measured. Liver microsomes prepared within 24 h displayed similar ethoxyresorufin O-deethylation, bufuralol 1'-hydroxylation (BF 1'-OH), and chlorzoxazone 6-hydroxylation activities in all preservation solutions, whereas S-mephenytoin 4'-hydroxylation and midazolam 1'-hydroxylation activities displayed some variation depending on the preservation buffer used. Most of the marker P450 activities were stable for 3 days after removal from surgical patients and declined at 7 days; however, a decline in BF-1'OH activity was observed even at day 3. These results suggest P450-specific activity depends on the method by which human liver samples are preserved. Moreover, the results of these studies establish the minimum tissue preservation criteria that, when met, qualify the drug disposition data generated from subcellular fractions derived from a particular surgical tissue sample.

Buffers↗
PubMed1980

[Biotension of the corneal tissue preserved in a nutrient medium for tissue cultures at 4 degrees C].

The biotension generated by cells and tissues is connected quite well with the intensity of the metabolic processes in them and in seems that it is affected considerably by a series of external factors-temperature, salt content ect. For the purposes of transplantation in the present investigation the authors examined the effect of the nutritive medium for tissue cultures T-199 on the investigated biotension of the corneal tissue during the period of its preservation. They carried out studies on 335 corneas and the results were estimated statistically. The mean rate of a change on the biotension for the single series of investigation was determined. He found that the measured biotension was affected bu the time and conditions of preservation of the corneal tissue, when he compared the dispersion in the values of the tension after the preservation of the tissue in two media moist chamber and nutritive medium for tissue cultures). The nutritive medium used probably influenced positively the rate of the adaptation processes in the tissues after its separation from the ocular buebus, delayed the processes of deterioration and to a large extent stabilized the measured biotension.

Animals↗
PubMed1983

Parallel growth of rectus sheath grafts and recipient aorta. Critical role of graft tissue preservation.

Previous experimental studies revealed striking similarities in mechanical properties of host aortic tissue and rectus sheath inserted as an aortic graft, as well as enlargement of some of the grafts. We have now evaluated the capacity of rectus sheath grafts to keep pace with aortic growth and investigated the role of tissue preservation in determining the ability of such grafts to maintain suitable dimensions. Autologous rectus sheath grafts were substituted for thoracic aortic segments in 15 puppies, 8 weeks of age. Nine grafts were inserted with careful attention to tissue preservation (live grafts), but in six experiments, cells were killed and tissues fixed by freezing and thawing in acetone before graft implantation (devitalized grafts). Grafts were fixed in situ by controlled pressure perfusion and examined 1, 2, and 3 months after implantation. Over the 3 month experimental interval, body weight increased from 2.0 +/- 1 pounds to 25.0 +/- 2.5 pounds, and as is characteristic for the period of maximum growth rate for the animals. Total thoracic aortic length increased 41.2% +/- 6.1% and aortic diameter, 30.0% +/- 3.1%, during this time. Growth rate of the total thoracic aortic length during the first month was greater for the devitalized graft group, but aortic length was the same for both groups at 3 months. The live grafts increased 42.3% +/- 12.0% in length and 27.3% +/- 3.0% in diameter, i.e., to the same degree as the aorta, whereas the devitalized grafts showed only slight increases in length and diameter (2.1% +/- 2% and 5.3% +/- 5%, respectively). The live rectus sheath grafts were augmented and largely replaced by a newly formed, layered fibrocellular structure, 45.8 +/- 25.8 mm3 in volume, whereas in the devitalized grafts more of the original rectus sheath persisted and only a minimal amount of organized connective tissue sheath was formed (volume 18.7 +/- 8.9 mm3). In addition, cellularity was much greater in live grafts than in devitalized specimens (990.9 +/- 186.8 cells/mm2 and 423.6 +/- 124.7 cells/mm2, respectively). All of the grafts were lined by endothelium. These findings indicate that autologous rectus sheath aortic grafts may be used to provide channels which keep pace with aortic dimensions during the period of maximum growth, provided that the graft tissue is viable on implantation. Long-term durability of such grafts may also depend on tissue preservation.

Abdominal Muscles↗
PubMed1998

[Comparative study on DNA abstraction of human tissues preserved by two different methods].

Comparative studies have been made on the effect of DNA abstraction of different human tissues preserved by different methods. The results showed that different human tissues preserved in 50% alcohol may have similar DNA abstraction effect for a relatively long time as frozen tissues. They have no significant difference (chi 2 < or = 0.21, P > or = 0.995). This preservation method has the advantage of simplicity, high DNA output and good effect, and is suitable for preserving tissues under special circumstances.

Alcohols↗
PubMed2002

Liver surgery in the era of tissue-preserving resections: early and late outcome in patients with primary and secondary hepatic tumors.

During recent decades, the understanding of the segmental-oriented liver anatomy has enabled development of resection of isolated liver segments or sectors as dictated by the localization and extent of the mass lesion. These newer surgical procedures provide the advantage of maximal preservation of functional parenchyma, therefore minimizing the occurrence of postoperative liver failure and, at the same time, expanding the indications for surgery. We analyzed the results after classical hemihepatectomies and segment-based resections in a consecutive, nonselected patient group. During the 7-year period between November 1993 and November 2000, 270 patients with primary or secondary liver tumors were treated in our hospital; 167 of these patients underwent curative resections and their relevant data were entered into a statistical database. There were 77 classical hemihepatectomies and 90 tissue-preserving resections. Total mortality and morbidity for the series was 3.6% and 29.9%, respectively. Morbidity but not mortality was significantly lower after tissue-preserving resections than after classical hemihepatectomy. Median follow-up was 36 months. Survival was comparable for the two different surgical approaches for patients with secondary liver malignancies. In contrast, patients with hepatocellular carcinomas lived significantly longer after tissue-preserving resections. Tissue-preserving liver resection is a safe technique allowing maximal preservation of functional parenchyma without compromising radicality. Therefore, tissue-preserving resection is especially useful for patients with hepatocellular carcinomas and cirrhosis.

Adolescent↗
PubMed2003

Ionic liquids in embalming and tissue preservation. Can traditional formalin-fixation be replaced safely?

Ionic liquids (ILs) can be used for embalming and tissue preservation. ILs does not cause tissue damage and the tissue colour remains unaltered after treatment. Microscopical morphology of tissues fixed in ILs is of better quality than that of tissues fixed in formalin. Tissue preservation depends on the type of ILs. Best results were obtained with 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate, the density of which resembles that of water. The salt is nonvaporous and when used as a formalin substitute, it eliminates health hazards in the pathological laboratory.

Animals↗