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Macromineral absorption in sheep fed tetany-prone and non-tetany-prone hays.

Two experiments were conducted to determine some of the factors that led to hypomagnesemic tetany associated with the feeding of two orchardgrass hays. Sixteen mature Columbia and Suffolk wethers (62 to 72 kg), four of which were fitted with ruminal and abomasal cannulae, were fed one of two tetany-prone orchardgrass hays or a non-tetany-prone bromegrass hay. In Exp. 1, 12 wethers were used in a completely random design metabolism experiment to measure apparent absorption and retention of macrominerals. In Exp. 2, four wethers with ruminal and abomasal cannulae were used in a completely random design experiment to monitor pre-intestinal mineral absorption and ruminal characteristics. This experiment was replicated once, with wethers remaining on their diets for 16 d in each replication. In the metabolism experiment, apparent absorption and retention of Mg as a percentage of intake were lower (P less than .01 and P less than .05) for one tetany-prone orchardgrass hay compared with the other orchardgrass hay. Pre-intestinal absorption of Mg in the cannulated wethers was greater (P less than .01) for the orchardgrass hays than for the bromegrass hay. Pre-intestinal Mg absorption was higher (P less than .01), both in terms of grams per day and a percentage of intake for the orchardgrass hay with the highest Mg content. In the noncannulated wethers, the percentage of water-soluble Mg in the feces was lower (P less than .01) for the tetany-prone hays, indicating that a decrease in Mg solubility in the intestine may have influenced Mg apparent absorption.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals

Fatigue of isolated mouse muscle due to isometric tetani and tetani with high power output.

The fatiguing effect of a series of isometric tetani and of tetani with shortening and maximum power output were compared using soleus and extensor digitorum longus muscle of mouse. Mean force in test isometric tetani after the series was reduced by up to 35% compared with pre-series controls. Tetani with shortening were always more fatiguing than isometric tetani.

Animals

[Standardization of the electromyographic tetany test in the diagnosis of normocalcemic tetany: the 10-minute Trousseau test in patients and healthy humans].

Eighteen adult patients with typical history and signs of normocalcemic tetany (hyperventilation syndrome) and 18 age- and sex-matched controls were submitted to a provocation test for tetany. This consisted of 10-min ischemia of the right arm produced by suprasystolic cuff compression at the upper arm (Trousseau test) and a postischemic electromyographic recording from the right interosseous I muscle. Postischemic repetitive discharges were found in all patients and predominantly consisted of multiples of 5 times and more. These discharges lasted 8 min on the average. Of the normal controls, as many as 16 subjects (88.8%) also exhibited tetanic discharges. However, these preferred a lower number of repetitions, such as doublets or triplets. Both number of repetition and duration of postischemic tetanic activity were significant parameters discriminating between patients and controls (2P less than 0.001), as was the cumulative total number of spontaneous potentials, amounting, on the average, to 10,266 in the group of patients and to only 320 in the controls (2P less than 0.001). The investigation has shown that the occurrence of postischemic tetanic spontaneous activity per se does not help to discriminate between cases of latent tetany and healthy controls. However, a normocalcemic tetanic condition may be assumed if multiples occur with a complexity of quadruplets or more, if these multiples last at least 2 min, and if they appear in a rhythmic order and in groups.

Adult

Wheat pasture poisoning. II. Tissue composition of cattle grazing cereal forages and related to tetany.

Thirty-two aged Hereford and Angus cows (avg 10.2 yr old) grazed mixed cereal (wheat and rye) pastures from December 6, 1979 to April 23, 1980 (138 d). On d 104 (March 19), five cows developed tetany. On the day of tetany, the mean plasma Ca of the cows with tetany was 4.1 mg/dl. Cows with tetany had plasma Mg concentrations of 1.7 mg/dl on the day of tetany, but 15 d before tetany and 6 d post-tetany, levels were lower (1.2 mg/dl). Cows with tetany had large increases in plasma parathyroid hormone (PHT) as well as vitamin 1, 25 dihydroxy D3 [1,25(OH)2D], plasma glucose, lactic acid and beta-hydroxybutyric acid. Plasma K of these cows followed a similar trend as plant K, except it was not as pronounced. Plasma inorganic P of these same cows was exceedingly low, less than 3 mg/dl several times, although the forage P did not indicate a P deficiency. Levels of plasma hydroxyproline in these cows indicated that bone resorption may have been low during most of the grazing season, even though levels of plasma hydroxyproline were initially high and high subsequent to tetany. High levels of plasma glucose and lactic acid were associated with the time of tetany. Apparently, cows developed inappetence around the time of tetany, as suggested by the high levels of plasma beta-hydroxybutyric acid. Cows with wheat pasture poisoning appeared to respond to the severe hypocalcemia with large increases in plasma 1,25(OH)2D. Plasma 1,25(OH)2D changes were similar to plasma hydroxyproline changes (and possible bone resorption rates) and seemed inversely proportional to plasma Ca and directly proportional to plasma PTH concentrations. Generally, cerebrospinal fluid (CSF) composition was not affected as much as plasma composition, except for the low levels of CSF Ca. These data indicate that a metabolic deficiency of Ca (hypocalcemia) was the cause of tetany.

3-Hydroxybutyric Acid