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At least 19 recordsLinked to original sources
PubMed1958

Studies on the effect of triton (WR-1339) on guinea pig tissues. III. Tuberculin hypersensitivity and in vitro cytolysis of leucocytes by tuberculin.

The administration of triton WR-1339 (300 mg./kg. subcutaneously) to tuberculin-sensitive guinea pigs 2 hours before the intradermal injection of PPD depresses slightly their skin sensitivity to tuberculin and essentially obliterates the lytic effect of tuberculin on their circulating leucocytes. When leucocytes from tuberculin-sensitive guinea pigs are exposed to triton in vitro at a concentration level attainable in vivo the cells are partially protected against lysis by PPD.

Animals↗
PubMed1958

Inhibition of tuberculin skin hypersensitivity in guinea pigs by injection of tuberculin and intact tubercle bacilli during fetal life.

FEMALE GUINEA PIGS WERE SUBJECTED TO LAPAROTOMY AT DIFFERENT STAGES OF PREGNANCY, AND THEIR FETUSES INJECTED THROUGH THE UTERINE WALLS WITH ONE OF THE FOLLOWING PREPARATIONS: Old Tuberculin, tubercle bacilli of the BCG strain killed by heat or exposure to phenol, and living BCG. A large number of the animals injected in utero with Old Tuberculin failed to develop skin hypersensitivity to P.P.D. following vaccination with 200 or 800 gamma phenol-killed tubercle bacilli (BCG) in early adulthood. Normal control animals were sensitized by vaccination with such quantities of phenolized BCG. The failure of animals which had been injected with Old Tuberculin in fetal life to respond hypersensitively to P.P.D. after adult vaccination with tubercle bacilli is ascribed to their acquisition of a state of immunological tolerance to tuberculoprotein (tuberculin tolerance). Fetal injection with killed BCG conferred a state of tolerance on a few of the animals, and rendered others tuberculin-sensitive. Fetal injection with living BCG sensitized most of the animals to tuberculin, even when fetal exposure was as early as 46 days before birth, and induced tolerance in none. Fetuses of the same litter, injected simultaneously with identical inocula, often responded differently, some becoming tolerant to tuberculin, others developing hypersensitivity, and still others remaining immunologically unaffected, becoming neither sensitive nor tolerant. The state of tuberculin tolerance induced in these experiments was limited. When tolerant animals were revaccinated with living BCG several weeks after vaccination with phenol-killed bacilli, they developed as high a degree of tuberculin skin sensitivity as the originally non-tolerant animals.

Animals↗
PubMed1953

Effect of infection with M. tuberculosis and of tuberculin shock on the succinic dehydrogenase activity of guinea pig tissues.

The kidney of guinea pigs infected with the H37Rv and BCG strains of M. tuberculosis showed a diminution in succinic dehydrogenase activity when measured by the tetrazolium technique. This effect was also seen in the liver and spleen of animals infected with the BCG strain. Sensitized animals showed similar results when given tuberculin in sublethal doses. The succinic oxidase was also low in the kidneys of animals infected with the H37Rv strain. The depressed enzyme activity of the tissues of infected animals could be restored to normal by addition of normal tissue extract or dialysate. This suggests that the alteration in tissue metabolism observed in tuberculosis may depend upon the loss of some as yet unidentified factor important for succinic dehydrogenase activity.

Animals↗
PubMed1953

The effect of tuberculin and cortisone singly and in combination on explanted tissues of guinea pigs, mice and rabbits.

Tuberculin (P.P.D.) and cortisone acetate, singly and in combination, were added to explanted splenic tissue derived from guinea pigs, mice, and rabbits, and buffy coats obtained from rabbits. These tissues came from normal animals or from animals which had been infected with the tubercle bacillus or immunized with killed tubercle bacilli. 5 microg./ml. of tuberculin or 0.5 microg./ml. of cortisone was the largest concentration of the respective reagents which was tolerated by cultured cells from normal animals. From the results with all tissues studied it would appear that P.P.D. selectively damaged only the growing cells of splenic tissues from sensitive guinea pigs and to a lesser degree the migrating cells of buffy coats obtained from sensitive rabbits. Cortisone appeared to have increased toxicity for only explanted splenic cells from tuberculin-sensitive guinea pigs. The specific effect of P.P.D. on tissues from tuberculin-sensitive animals was not modified by cortisone under the conditions of these experiments. Thus these data furnished no evidence that cortisone had any direct effect on the response of the sensitive cells to P.P.D.

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