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At least 19 recordsLinked to original sources
PubMed1983

Antigen-specific proliferation responses of peripheral blood lymphocytes to Trichomonas vaginalis antigen in patients with Trichomonas vaginitis.

This report describes the development of an assay system which overcomes the difficulty of detecting immune responses of patients with Trichomonas vaginitis by making use of peripheral blood leukocytes obtained from such patients. When peripheral blood leukocytes from the patient were stimulated in microcultures with the soluble antigen extracted from Trichomonas vaginalis, significant degrees of proliferation ensued, as measured by the incorporation of [methyl-3H]thymidine 4 to 5 days after initiation. The antigen-induced proliferation response of peripheral blood leukocytes is specific for T. vaginalis antigen. The T. vaginalis-specific [methyl-3H]thymidine incorporation is mediated by Leu-1-positive cells, namely, T lymphocytes, in the peripheral blood leukocyte population. This assay system should prove useful for the analysis of the immune response to the protozoa in patients with Trichomonas vaginitis.

Antigens↗
PubMed1994

Identification of the lipid moiety and further characterization of the novel lipophosphoglycan-like glycoconjugates of Trichomonas vaginalis and Trichomonas foetus.

The lipid moiety of the lipophosphoglycan (LPG)-like glycoconjugates of Trichomonas vaginalis and Trichomonas foetus, parasites of the urogenital tract of human and cattle, respectively, has been isolated and characterized by a combination of enzymatic and chemical degradation, chromatography, and mass spectrometry. The carbohydrate composition of the glycan inositol lipid core is also reported. The glycan inositol core of trichomonad glycoconjugates is unique in having more than one GlcN and is significantly larger than any other glycan core reported so far. T. vaginalis glycoconjugate binds strongly to the lectin RCA-I, which suggest that the macromolecule possesses terminal beta 1,4-linked galactosyl residues. The binding of T. foetus glycoconjugate to the lectin UEA-I suggests the presence of terminal alpha 1,2-linked fucose. Acid hydrolysis of deaminated and reduced LPG products yields a [3H]anhydromannitol-containing product, indicating the presence of unacetylated glucosamine in the trichomonad LPGs. Reductive radiomethylation has been applied to label free amino groups in the hexosamine or other free amine-containing residues of the trichomonad glycoconjugates. Treatment of the LPGs with phosphatidylinositol-specific phospholipase C from Bacillus thuringiensis liberates a ceramide substituent. Treatment of LPGs with nitrous acid releases a phospholipid moiety containing myo-inositol and ceramide, implying that the LPGs are anchored in the membrane via an inositol-phosphate-ceramide. Structural characterization of the ceramide by gas-liquid chromatography (GC) and GC-mass spectrometry indicated the presence of the major long-chain base sphinganine (d 18: 0 dihydrosphingosine) and a C 16:0 N-acyl group. Lipophosphoglycans from both parasites contain ceramide as their only lipid moiety. These results suggest that T. vaginalis and T. foetus anchor their LPG-like glycoconjugates on the cell surface via inositol-phosphoceramide and also the glycan inositol core of the macromolecule appears to be unique in nature.

Animals↗
PubMed1973

[Fine Structures Of Trichomonas Tenax And Trichomonas Hominis]

Trichomonas tenax(T. tenax) and Trichomonas hominis (T. hominis) were collected, cultured and sampled for comparative microscopical studies using electron microscope. 1. Both flagellates were oval in shape and surrounded by a distinct outer membrane. Five recurrent flagella and one anterior flagellum had, each, 9 paris of peripheral and 1 pair of central fibrils, Undulating membrane was curved over the recurrent flagella, and bended in the middle at right angles with cell surface. Cytostome, engulfing bacteria, was observed in T. hominis. 2. In the cytoplasm, there were fine dense glycogen particles, and vacuoles containing ingested materials. Dense pigment rods were also observed in both flagellates, but the rods were not distributed around the vacuoles in T. hominis. 3. In T. tenax axostyle appeared as a cup-shaped structure comprising a single row of 41 fibrils, each about 120 a in diameter. It enclosed glycogen particles, and the open side was faced to the nucleus. 4. Endoplasmic reticulum was observed around the nucleus, but it was less developed in T. hominis. 5. Nucleus was ovoid having double nuclear membrane, which was clearly defined in T. hominis. 6. Blepharoplast, parabasal body, Golgi appartus and mitochondrion was not observed in both flagellates.

Journal Article↗
PubMed1991

[Virulence of Trichomonas vaginalis strains in relation to clinical forms of Trichomonas vaginitis].

Estimation of virulence of T. vaginalis strains injected subcutaneously to mice was done. T. vaginalis strains were obtained from the urogenital tracts of patients with different clinical forms of trichomoniasis. The virulence of T. vaginalis strains measured by infiltration size was found to depend upon a clinical form of trichomoniasis . It was moreover shown that the cutaneous infiltration size is proportional to a dose of Trichomonas administered subcutaneously. Histopathological analysis of cutaneous infiltration itself is not decisive as to the strains virulence, yet in addition to macroscopic examination it may be a criterium in the studies on strain pathogenicity.

Acute Disease↗
PubMed1993

The divergence of Trichomonas vaginalis virus RNAs among various isolates of Trichomonas vaginalis.

Trichomonas vaginalis virus is a double-stranded (ds) RNA virus found in the parasitic protozoan T. vaginalis. To examine the possible existence of related viruses in various T. vaginalis strains and isolates, we screened 20 such isolates and found the characteristic 5.5-kb viral ds RNA in 16 of them. An additional 0.5-kb ds RNA band was also identified in 10 of these virus-infected T. vaginalis isolates. In a local isolate T1, this 0.5-kb ds RNA cross-hybridized with the 5.5-kb ds RNA. Preliminary evidence from CsCl buoyant density gradient centrifugations suggests that the two ds RNA species are encapsidated in separate viral particles by the same capsid protein. The 5.5-kb ds RNA in isolate T1 exhibited moderate hybridization with the prototype 5.5-kb ds RNA in T. vaginalis stratin NIH-Cl. Little cross-hybridization was observed among the 5.5-kb ds RNAs from T. vaginalis isolates JH32A No. 4, T1 and T5, although the viral proteins in these isolates are of similar molecular weight and share common epitopes as viewed by Western blotting. Hybridization characteristics of the 5.5-kb ds RNAs in individual isolates were further analyzed, and the results suggest great ds RNA divergence among the T. vaginalis viruses. Interestingly, the 0.5-kb ds RNA in different isolates of T. vaginalis also exhibited divergence in patterns closely associated with that of the 5.5-kb ds RNA.

Animals↗
PubMed1975

[Cultivation of Trichomonas gallinarum and Trichomonas tenax on a trimed medium].

Studied was the possibility to isolate and culture Tr. gallinarum and Tr. tenax on a medium Trimed, proposed by the authors for the isolation and cultivation of Tr. vaginalis. The growth and development of the two Trichomonas species were followed up at various temperatures -- 38 degrees, 36 degrees, and 32 degrees C, in order to establish the most appropriate temperature for continuous cultivation at longer intervals of reseeding as well as the temperature optimum for the fast deposition of great amounts of biomass. It was found that the Trimed medium is suitable for the isolation and cultivation of Tr. gallinarum and Tr. tenax. Temperatures of 38 degrees and 36 degrees contribute to the accumulation of greater amounts of biomass, while at 32 degrees C the growth of these protozoa is delayed and reseeding is to be carried out at greater intervals.

Animals↗