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Treatment of Salmonella-Arizona-infected turtle eggs with terramycin and chloromycetin by the temperature-differential egg dip method.

Attempts to eliminate Salmonella and Arizona infection from newly hatched turtles were made by dipping fresh eggs in cold solutions of Terramycin and Chloromycetin at 1,000, 1,200, 1,500 and 2,000 mug per ml for either 10, 20, or 30 min. Control groups consisted of hatchings produced from nondipped eggs or eggs dipped in chilled water. In two of the four experiments 5 to 10 eggs were blended on days 15, 30, and 45 post antibiotic dip treatment. Twenty-five to 60 hatchlings from each control or experimental dip groups were held in containers and the water was tested (excretion method) for Salmonella and Arizona every 15 or 30 days for 180 to 210 days after hatching. Representative turtles were homogenized (blending method) to determine if systemic infections were present. All specimens tested were enriched in tetrathionate and selenite cystine broth. Nondipped eggs and water-dipped eggs routinely showed Salmonella and Arizona present in egg homogenate and hatchlings emerging from these eggs excreted these pathogens. Terramycin- and Chloromycetin-dipped eggs were uniformly negative for these pathogens, only if fresh eggs were dipped. Bacteriological assay of container water and whole turtle homogenate from hatchlings were negative for Salmonella and Arizona if eggs were dipped in 1,000 mug of Terramycin early in the egg laying season or if eggs were dipped in 1,500 or 2,000 mug of Terramycin per ml late in the egg laying season. The results of temperature-differential egg dip studies suggest that this is a feasible and promising method by which to eradicate Salmonella and Arizona from the turtle.

Animals↗

Terramycin in urinary tract infections.

Terramycin(R) is an effective agent in the control of urinary tract infections with organisms of the enteric group. The drug is tolerated by mouth and no serious side-reactions occur. In cases in which there is no organic or obstructive disease, the response to Terramycin as a urinary antiseptic is prompt and effective. In several cases in which there was severe organic and obstructive disease and the organism was highly resistant, the course of the disease was not altered by giving Terramycin.

Anti-Infective Agents, Urinary↗

Adaptation of Bacillus megatherium to terramycin (oxytetracycline).

B. megatherium cultures contain a few cells per hundred million which are able to grow in the presence of terramycin. Growth of the other cells is inhibited by the antibiotic with the result that the resistant cells overgrow the culture. The resistant cells are present in the culture, without contact with terramycin, and are able to grow in the presence of terramycin, without further modification. The resistant cells are mutants of sensitive cells.

Bacillus megaterium↗

Effect of terramycin in balantidiosis of pigs.

In an industrial pig farm in Lubumashi, Zaïre, Balantidium coli produced severe clinical and fatal disease. Terramycin at a dose rate of 15 mg/kg body weight administered twice daily with concentrates gave clinical recovery in all the pigs treated. The complete parasitological recovery was obtained in 14 out of 20 animals. In the remaining 6 there was significant reduction in the number of B. coli. Keeping in view the large spectrum of activity of terramycin in various infections as well as B. coli, terramycin can be useful in the treatment of balantidiosis of pigs.

Animals↗

[Anaerobic hydrolysis of terramycin crystallizing mother solution].

The terramycin crystallizing mother solution contained high organics and high nitrogen. There were many kinds of bioinhibition in it but not enough electronic donor. Anaerobic hydrolysis of terramycin crystallizing mother solution was completed with up anarobic sludge bed in order to improve the biodegradability of wastewater and electronic donor in it. The variations of pH, COD, NH4+, and SO4(2-) were monitored. The COD removal was in a narrow range between 10% and 16.4% even when the HRT of the reactor was changed from 1.5 h to 6 h. pH increased because of formation of NH3 and reduction of SO4(2-). Most of SO4(2-) was reduced to S2- when the HRT was longer than 2 h. Batch experiments on hydrolyzed wastewater demonstrated that reaction rates of nitrification and denitrification increased by 90.9% and 45.2%, respectively.

Anaerobiosis↗

A dermatophilosis outbreak in southern Sudan treatment trial with terramycine long activity.

Dermatophilosis is a contagious enzootic disease in tropical areas but which sporadically appears in temperate countries. During an outbreak in Sudan, the resistance level of the Nilotic cattle (Dinka breed) and various crosses has been recorded. The use of Long Acting Terramycin for treating infected cattle is discussed as well. A single injection of Long Acting Terramycin (20 mg/kg) is curative in 35.9% of the cases. The less severe the infections are, the best results are obtained. Treatment response does not seem modified by the breed of the treated cattle.

Actinomycetales Infections↗

The proportion of terramycin-resistant mutants in B. megatherium cultures.

The number of terramycin-resistant mutants in Bacillus megatherium cultures, their mutation rate, and the growth rate of the wild and mutant cells have been determined under various conditions. These values are in agreement with the following equations (Northrop and Kunitz, 1957):- See PDF for Equation lambda = mutation rate, A = growth rate constant of wild cells, B = growth rate constant of mutants, See PDF for Equation equilibrium. The value of the mutation rate as determined from equation (6) agrees with that found by the null fraction method.

Bacillus megaterium↗