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At least 19 recordsLinked to original sources
PubMed1999

An ultrastructural study of the biostimulative effect of He-Ne laser radiation on the early postnatal differentiation of the rat submandibular gland.

Submandibular gland of the rat during the first week of postnatal development was used for the study of the ultrastructural changes after the He-Ne laser radiation. In the experimental group we observed an increase of the dark cells with greater quantity of the granular endoplasmic reticulum and an increased number of specific secretory granules. These changes show positive biostimulative effect of He-Ne laser radiation on the morphologic differentiation of the submandibular gland of the rat in the early postnatal period.

Aging↗
PubMed1987

Ultrastructure of the ferret submandibular gland.

Submandibular glands of two male and two female adult ferrets were examined using electron microscopy. The secretory endpiece consisted of mucous acini and seromucous demilunes. The acinar cells were filled with mucous granules of moderate electron density. Each granule had a fine fibrillar substructure. Two types of demilune cells could be identified, based on the differing electron density of their secretion granules. In some cells the granules were more electron-dense than in others. These granules may contain the two types of mucosubstances identified in an earlier study. The demilunes probably drained into the intercellular canaliculi present in between them and eventually into the acinar lumen through a demilune projecting into it. The intercalated ducts were shorter compared to those of the parotid glands. Many of the striated duct cells contained electron-dense secretion granules. The basal plasmalemma of these cells was invaginated to form linear folds. Few agranular cells were interspersed among the granulated cells of the striated ducts.

Animals↗
PubMed1985

Effects of hypothyroidism on the DNA, carbohydrate, soluble protein and sialic acid contents of rat submandibular glands.

Submandibular glands are a target organ of thyroid hormones. This study examined the effects of hypothyroidism on the biochemical characteristics of these glands in the rat. There were no effects on the neutral sugar and DNA contents. However, soluble protein concentrations (micrograms/mg wet weight) were significantly decreased and sialic acid concentrations micrograms/mg soluble protein) were significantly elevated.

Animals↗
PubMed1991

Malignant epithelial tumors in the minor salivary glands, the submandibular gland, and the sublingual gland. Prognostic factors and treatment results.

Ninety-five malignant tumors in the submandibular gland, the sublingual gland, and the minor salivary glands seen in a 25-year period were reviewed. The patients were retrospectively staged using the Union Internationale Contre le Cancer (UICC) classification. The most frequent tumor was adenoid cystic carcinoma, followed by adenocarcinoma. The submandibular gland was the most frequent location. Five-year and 10-year crude survival rates were 62% and 43%, respectively. Clinical stage was the most important prognostic factor. Survival was not correlated with location of tumor, although recurrence and metastases occurred more frequently in patients with cancer of the submandibular gland. Histologically, the 5-year and 10-year survival was significantly better for patients with adenoid cystic carcinoma compared with the other types; however, although still significant, this difference diminished at 10 years, confirming the need for a long observation time for patients with this tumor.

Adolescent↗
PubMed1985

Differential actions of testosterone and its metabolites on mice submandibular gland.

Submandibular glands from male mice castrated at 21 days of age and killed 60 days thereafter exhibited impaired development of the granular ducts (GD), an effect which is directly related to its androgen-dependent feature. Testosterone and other related steroids of natural occurrence were given to these animals in order to determine the mechanism by which those compounds bring about recovery of their glandular histophysiology. The size and number of GD were reconstituted by the steroids in the following order of potency: 5 alpha-androstane-3 alpha, 17 beta-diol greater than testosterone propionate greater than 5 alpha-dihydrotestosterone greater than or equal to testosterone greater than 5 alpha-androstane-3 beta, 17 beta-diol. All of the drugs stimulated protein synthesis, but only 3 alpha-diol was also able to increase DNA synthesis. Results support the assumption that 3 alpha-diol exerts both proliferative and hypertrophic effects on mice SMG, possibly through some receptor-independent pathway(s); the action of the other steroids is essentially hypertrophic in nature.

Androstane-3,17-diol↗
PubMed1992

[Peptidergic innervation of human salivary glands (parotid gland and submandibular gland)].

Immunocytochemistry and a radioimmunoassay were used to investigate the existence and distributions of various regulatory peptide immunoreactivities (ir) in human submandibular and parotid glands. Numerous nerve fibers containing vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM), or neuropeptide tyrosine (NPY) and C-flanking peptide of NPY (CPON)-ir were found in close proximity to acini, ducts and blood vessels. Only a few calcitonin gene-related peptide (CGRP)- and substance P (SP)-ir nerve fibers could be demonstrated and were mainly localized around blood vessels and ducts. Galanin and the recently discovered peptides helospectin and pituitary adenylate cyclase activating peptide were unable to be detected in the salivary glands studied. Preliminary quantitative investigations of four human submandibular glands using radioimmunoassay showed that VIP-ir had the highest concentration, followed by NPY-ir and CGRP-ir; SP-concentrations were below the detection limit. The possible physiological significance of these peptides for salivary secretion is discussed.

Adenoma, Pleomorphic↗
PubMedDate not supplied

Submandibular gland peptide-T (SGP-T) modulates ventricular function in response to intravenous endotoxin.

A novel peptide hormone isolated from salivary glands, submandibular gland peptide-T (SGP-T), protects against the enhanced hypotensive response to intravenously administered lipopolysaccharide (LPS; 3.5 mg/kg; Salmonella typhosa) in rats with their submandibular glands removed (sialadenectomy). In this study, we examined the effects of SGP-T on LPS-provoked perturbations of heart function. Sialadenectomy did not alter basal heart function, although the sialadenectomized rats exhibited more pronounced reductions in ventricular peak systolic pressure (VPSP), ventricular pressure at max dP/dt (Pmax dP/dt), and maximum ventricular negative dP/dt (-dP/dt) relative to unoperated controls following LPS challenge. These changes were primarily due to changes in afterload (blood pressure). However, in sialadenectomized rats LPS-induced changes in Pmax -dP/dt (ventricular pressure at maximum -dP/dt) did not correlate with changes in VPSP, which suggests that sialadenectomy may alter the systolic component of the heart beat. The peptide SGP-T, at doses of 1 and 3.5 microg/kg, corrected Pmax -dP/dt and all other endotoxin-induced changes in heart function. Since Pmax -dP/dt is a measure of relaxation during diastole, the submandibular glands appear to play a role in protecting the heart against the adverse effects of acute endotoxin administration on ventricular relaxation. These effects of the submandibular glands may be mediated by the release of the peptide SGP-T.

Animals↗
PubMed1998

[Reconstructive lacrimal gland with free submandibular gland transfer for management of xerophthalmia].

OBJECTIVE: In order to seek more efficient surgical method to treat total xerophthalmia. METHOD: Microvascular submandibular gland transfer was used to reconstruct lacrimal gland for management of the dry eye. 5 cases (6 eyes) with total xerophthalmia were operated with this method. RESULTS: All the patients were followed up for 1 - 30 months. The results were satisfactory, the submandibular salivary gland relieved the dryness and improved visual acuities with negligible side effect. CONCLUSION: Microvascular submandibular gland transfer is potentially the best surgical solution to total xerophthalmia.

Adolescent↗
PubMed1999

Immunocytochemical detection of estrogen and progesterone receptors in the rabbit submandibular gland.

Rabbit submandibular glands produce secretions involved in olfactory communication. The histology of these glands and their secretory activity are: sexually dimorphic; vary across the female reproductive cycle; and are modified by gonadectomy. This suggests that gonadal steroids regulate the structure and function of such glands. To further support this idea we assessed by immunocytochemistry the presence of estrogen and progesterone receptors in male and female rabbit submandibular glands. Immunoreactivity was detected only in the nucleus of acini cells. The number of estrogen receptor-immunoreactive cells/field varied among estrus (26 +/- 6; mean +/- S.E.), ovariectomized (19 +/- 2), and ovariectomized-estrogen-treated animals (13 +/- 3). Intact males showed a significantly smaller number of estrogen receptor-immunoreactive cells/field (12 +/- 1) than estrous females. Interestingly, progesterone receptor-immunoreactive cells were more abundant in estrous (32 +/- 7) than in ovariectomized animals (7 +/- 1). Estradiol benzoate (5 micrograms daily for 5 days) increased the number of progesterone receptor-immunoreactive cells/field in ovariectomized females (17 +/- 1). Intact males showed fewer progesterone receptor-immunoreactive cells/field (16 +/- 2) than estrous females. Results show that the rabbit submandibular gland is a target for estrogen and progesterone and support the idea that these hormones participate in regulating the physiology of this gland.

Animals↗
PubMed1986

Morbidity study of submandibular gland excision.

Submandibular gland excision is an operation frequently performed by many different surgical specialists. It is often associated with a variety of postoperative complications, the commonest being neurological deficits related to damage to the marginal mandibular, lingual or hypoglossal nerves. Other patients develop further problems at a later date due to the development of calculi from gravel retained in the duct remnant. This study aims to discuss how these complications can be avoided.

Adolescent↗
PubMed1992

Characterization of a new kallikrein-like enzyme (KLP-S3) of the rat submandibular gland.

The submandibular gland of the rat contains several enzymes belonging to the kallikrein family. These include tissue kallikrein, antigen gamma (T-kininogenase), esterase B and tonin. In the present study, a new member of this family, which we have named KLP-S3, was identified and purified from the submandibular gland. KLP-S3 was classified as a kallikrein-like enzyme on the basis of its immunological similarity to other kallikrein-like enzymes and its showing 70% and 73% identity in partial amino acid sequence with tissue kallikrein and tonin respectively. Furthermore, the 44 sequenced amino acid residues showed complete correspondence to the mRNA S3 of the kallikrein gene family, which was the rationale for the name kallikrein-like protein (KLP) S3. KLP-S3 consisted of three isoenzymes with pI 6.75, 6.90 and 6.95, which significantly differed from those of other kallikrein-like enzymes. In conjunction with its immunological relationship to kallikrein, this parameter (pI) was considered robust enough to identify the enzyme during purification, since a specific physiological substrate for KLP-S3 has yet to be identified. In SDS/PAGE the three isoenzymes ran as one band with a molecular mass of 25,800 Da, which after reduction with 2-mercaptoethanol was split into two chains with molecular masses of 16,500 and 13,300 Da. In common with other kallikrein-like enzymes, KLP-S3 was inhibited by phenylmethanesulphonyl fluoride, and was thus classified as a serine protease. It was also inhibited by soya-bean trypsin inhibitor but not by aprotinin. It showed weak reactivity against the chromogenic substrates S2288, S2266, S2366 and S2302 (D-Ile-Pro-Arg 4-nitroanilide, D-Val-Leu-Arg 4-nitroanilide, Glu-Pro-Arg 4-nitroanilide and D-Pro-Phe-Arg 4-nitroanilide respectively) and did not cleave rat T-kininogen or dog high-molecular-mass/low-molecular-mass kininogen. Its specific angiotensin II-generating activity (angiotensin I as substrate) was 0.04% of that of rat tonin. KLP-S3 (1-100 nM) induced a statistically significant angiotensin-independent contraction of isolated rat aorta rings. The maximum contraction was 15% of the response to the alpha-adrenoceptor agonist phenylephrine (1 microM). The concentration of KLP-S3 in the rat submandibular gland was by single radial immunodiffusion estimated to be 47 +/- 3 micrograms/mg of protein.

Amino Acid Sequence↗
PubMed2000

The effect of ageing on parenchymal cell populations in adult female mouse submandibular gland.

The submandibular gland shows an array of responses that accompany ageing, which are usually modest. However, the submandibular acinar-cell mucin shows a substantial decline in total amount per gland. In the submandibular gland, there is also a loss of secretory parenchyma. A number of factors that could influence either parenchymal loss or a change in its cellular composition were examined in three ages of adult female mice. The goal was to see if there are ageing-related cellular changes that might have an effect on mucin production or secretion. The factors examined included DNA, protein, rates of cell division and apoptosis, cell volume and cellular composition of the parenchymal population. The parenchymal cell composition showed significant differences during ageing, with a substantial decrease in the percentage of acinar cells and increases in the percentage of both types of ductal cell components. This decline in the proportion of acinar cells in the parenchyma also reflected an overall reduction in the total number of acinar cells in the gland. Thus, the change in proportions of cells may potentially be a direct cause of the ageing-related decline in the submandibular acinar-cell mucin. The alteration in cellular composition was not attributable to changes in the cell-division indices; however, there was an increased rate of apoptosis for acinar cells that was significantly different between 3 and 28 months. The apoptotic rate doubled for acinar cells but showed no significant change in ductal cells. This selective change in the rate of apoptosis with ageing suggests that it is one of the main reasons for the decline in the proportion of acinar cells in the submandibular gland.

Aging↗
PubMed2000

Transduction of TAT-HA-beta-galactosidase fusion protein into salivary gland-derived cells and organ cultures of the developing gland, and into rat submandibular gland in vivo.

We have studied the transduction of TAT-HA-beta-galactosidase fusion protein into two cell lines of rat salivary gland origin, A5 and C6-21, into cells of fetal mouse submandibular glands in organ culture, and into rat submandibular gland after retrograde duct injection, using a histochemical method to demonstrate beta-galactosidase activity. Transduction of the fusion protein into A5 and C6-21 cells was concentration- and time-dependent. Therefore, the intensity of the beta-galactosidase staining, which was cytoplasmic, was less after 1 hr of exposure compared to exposures up to 24 hr. However, the fusion protein was transduced into 100% of both types of cultured cells. When explants of mouse fetuses at 13 days of gestation were exposed to the fusion proteins, both epithelial and mesenchymal cells were stained for the enzyme, with a conspicuous accumulation of the reaction product at perinuclear cytoplasmic regions. The histochemical staining of the mesenchymal cells was more intense compared to that seen in epithelial cells. TAT-HA-beta-galactosidase fusion protein was also delivered to rat submandibular glands by retrograde duct injection. Histochemical staining for beta-galactosidase activity of cryostat sections prepared from the injected glands revealed that the transduction of the fusion protein was also time- and dose-dependent. In the glands of rats sacrificed from 10 min to 1 hr after the retrograde injection, essentially all acinar and duct cells showed cytoplasmic staining. The intensity of the staining then declined, and was not seen in the glands of rats killed 24 hr after the injection of the fusion proteins. These results indicate that a full-length, active TAT fusion protein can be targeted to salivary gland cells both in vitro and in vivo to analyze physiological, developmental, and pathophysiological processes.

Animals↗