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Exchanges of magnesium and phosphorus at different sites in the ruminant stomach.

Three ruminating calves were each provided with a rumen cannula (from which reticulum samples could be obtained), an abomasal cannula and a sleeve sutured at the omasal-abomasal orifice that permitted digesta flowing from the omasum to be diverted and collected. The calves were each given in turn a high roughage (HR) and high concentrate (HC) diet consisting mainly of dried grass and flaked maize in the proportions by weight of 3:2 and 1:2 respectively. A water soluble non-absorbable marker (polyethylene glycol) and a particulate marker (103Ru-phenanthroline complex) were also given and the diets were sometimes supplemented with MgSO4. For each diet (HR(O), HR(Mg), HC(O), HC(Mg)) samples were taken from the reticulum and omasal outflow. Magnesium, phosphorus, PEG and 103Ru were measured in the samples as taken and liquid-rich and solid-rich fractions prepared from them. From their distributions relative to the two markers, Mg and P flows into and out of the omasum were estimated. Results were too few to show significant differences in absorption efficiency between diets but overall approximately 10-40% of the Mg entering the omasum was absorbed as a net effect implying that this organ may be the major site of Mg absorption in young cattle. For P also substantial amounts entering the omasum (approximately 10-40%) were absorbed in that organ.

Animals↗

H1- and H2-receptors in the smooth muscle of the ruminant stomach.

1 The effects of histamine on the longitudinal and circular smooth muscle from the rumen and reticulum of the bovine stomach have been analyzed by the use of H1- and H2-receptor antagonists. 2 Histamine caused three different types of response of the smooth muscle preparations: a contraction, contraction followed by relaxation, and a relaxation. These responses were resistant to the effect of tetrodotoxin, atropine, combined treatment with alpha- and beta- adrenoceptor blocking agents, hexamethonium and also to guanethidine. 3 Mepyramine antagonized the contractile responses and metiamide antagonized the relaxation responses in a highly selective and competitive manner. 4 It is concluded that histamine-induced excitatory and inhibitory responses of the smooth muscles from the bovine forestomach are mediated by histamine H1- and H2-receptors, respectively.

Animals↗