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Nematode larvae (Spirurida: Physalopteridae) causing infarction of the bowel in an infant.

A case is reported of an 11-month-old baby suffering from gangrene of the distal portion of the small bowel due to infestation by larval worms belonging to the order Spirurida, family Physalopteridae and probably the genus Physaloptera. Microscopically, the resected bowel showed ischaemic necrosis, eosinophilic granulomata and larval worms in the lumen and wall of blood vessels. The inflammatory process extended into the mesentery, where the vessels showed focal necroses, thrombosis and endarteritis. Infection in this child in the Queensland countryside is considered to have resulted from ingestion of insects on grass eaten when at play, the grass having been contaminated by bandicoots.

Humans

[Gongylonema pithyusensis n. sp. (Nematoda: Spiruridae), a parasite of the oesophagus of the dormouse Eliomys quercinus ophiusae Thomas, 1925 (Rodentia: Gliridae) in Formentera (Balearic Islands) (author's transl)].

A new Nematode, Gongylonema pithyusensis n. sp., parasitizing the oesophagus mucosa of the dormouse Eliomys quercinus ophiusae Thomas, 1925 in the balearic island of Formentera (Spain) is described. The male of the new species is characterized by the size of spicules and gubernaculum; the female by the morphology of the tail, the posteriour situation of the vulva and the dimensions of the eggs. It is the only Gongylonema species to be known parasitizing Rodents of the family Gliridae.

Animals

Granulomatous and neoplastic diseases of the skin of horses.

Lesions encountered in a clinicopathological study of cutaneous lesions resembling 'swamp cancer' from horses in North Queensland included 37 cases of subcutaneous phycomycosis, 5 of which were also infected with Habronema sp larvae. In addition 9 cases of primary cutaneous habronemiasis, 58 sarcoids, 12 fibromas, 8 fibrosarcomas, 10 squamous cell carcinomas, 4 haemangiomas, 3 melanomas, 1 papilloma, 1 palpebral adenocarcinoma and 7 cases of simple granulation were diagnosed. Subcutaneous phycomycosis resulted in the most extensive lesions. These were rapidly growing and provided the poorest prognosis of all conditions studied. The incidence of phycomycosis in north Queensland was high in comparison to more temperate areas and appeared to be related to the wetter seasons. Young horses were more commonly affected. A fungus isolated from 3 phycomycotic lesions was tentatively identified as Hyphomyces destruens as no sporulation was observed.

Animals

prot4EST: translating expressed sequence tags from neglected genomes.

BACKGROUND: The genomes of an increasing number of species are being investigated through generation of expressed sequence tags (ESTs). However, ESTs are prone to sequencing errors and typically define incomplete transcripts, making downstream annotation difficult. Annotation would be greatly improved with robust polypeptide translations. Many current solutions for EST translation require a large number of full-length gene sequences for training purposes, a resource that is not available for the majority of EST projects. RESULTS: As part of our ongoing EST programs investigating these "neglected" genomes, we have developed a polypeptide prediction pipeline, prot4EST. It incorporates freely available software to produce final translations that are more accurate than those derived from any single method. We show that this integrated approach goes a long way to overcoming the deficit in training data. CONCLUSIONS: prot4EST provides a portable EST translation solution and can be usefully applied to >95% of EST projects to improve downstream annotation. It is freely available from http://www.nematodes.org/PartiGene.

Animals

Critical tests of suspension, paste, and pellet formulations of cambendazole in the horse.

Three formulations of cambendazole were evaluated for anthelmintic activity by critical testing method in 21 horses. Cambendazole suspension was administered via stomach tube at the dose rate of 10 mg/kg to 3 horses and at the dose rate of 20 mg/kg to 3 horses. Cambendazole paste was given intraorally to 8 horses, and the pellet formulation was fed to 7 horses at the dose rate of 20 mg/kg. Anthelmintic activity of cambendazole was similar for all dose rates and formulations. Gasterophilus intestinalis, Gasterophilus nasalis, Draschia megastoma, Anoplocephala perfoliata, and Anoplocephala magna appeared to be refractory. The 3 formulations removed (1) 100% of Parascaris equorum and mature Oxyuris equi, (2) 82 to 100% of Strongylus vulgaris, (3) 80 to 100% of Strongylus edentatus, (4) 85 to 99% of small strongyles, and (5) 79 to 100% of immature Oxyuris equi. Probstmayria vivipara was present in only 1 horse and removal was 99%. There was some evidence of activity on Habronema muscae. Toxicosis was not observed.

Administration, Oral