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At least 19 recordsLinked to original sources
PubMed2003

Processing of sorghum (Sorghum bicolor) and sorghum products alters procyanidin oligomer and polymer distribution and content.

Sorghum procyanidins were characterized and quantified from two brown sorghum varieties and their processed products by normal phase HPLC with fluorescence detection. The DP of the procyanidins was determined by thiolysis. Quantification was done by using purified oligomeric and polymeric cocoa procyanidins as external standards. Sorghum procyanidins were composed mostly of high MW (DP > 10) polymers. Significant differences were observed in levels as well as distribution of the different MW procyanidins between the sorghums. Processing of the sorghum brans into cookies and bread significantly reduced the levels of procyanidins; this effect was more pronounced in the higher MW polymers. Cookies had a higher retention of procyanidins (42-84%) than bread (13-69%). Extrusion of sorghum grain resulted in an increase in the levels of procyanidin oligomers with DP </= 4 and decrease in polymers with DP >/= 6. This suggests a possible breakdown of the high MW polymers to the lower MW constituents during extrusion. Processing changes not only the content of procyanidins in sorghum products but also the relative ratio of the different molecular weights.

Biflavonoids↗
PubMed2003

Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products.

Specialty sorghums, their brans, and baked and extruded products were analyzed for antioxidant activity using three methods: oxygen radical absorbance capacity (ORAC), 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH). All sorghum samples were also analyzed for phenolic contents. Both ABTS and DPPH correlated highly with ORAC (R(2) = 0.99 and 0.97, respectively, n = 18). Phenol contents of the sorghums correlated highly with their antioxidant activity measured by the three methods (R(2) >or= 0.96). The ABTS and DPPH methods, which are more cost effective and simpler, were demonstrated to have similar predictive power as ORAC on sorghum antioxidant activity. There is a need to standardize these methods to allow for data comparisons across laboratories.

Antioxidants↗
PubMed2000

Canalization as a non-genetic source of adaptiveness during morphogenesis: experimental evidence from analysis of reproductive development in Sorghum bicolor.

In Sorghum bicolor, perturbations in reproductive development observed following salt-treatment also influence progeny grown in the absence of NaCl. However, a developmental reversion of these modifications may be observed throughout two successive generations. This response, termed canalization, does not spontaneously occur following growth in the absence of NaCl, but is triggered by the level of perturbation in parental expression of reproductive characters. Moreover, canalization is not specific to the perturbed character, but it includes modifications in reproductive development as a whole. A decrease in developmental variability coincides with amplitude of the developmental reversion. This phenomenon is interpreted as an evidence for orientation of the developmental process towards the lowest free-energy state of the 'epigenetic landscape'. Involvement of this phenomenon of canalization in developmental stability, adaptiveness, and evolution is discussed. Moreover, these results point to the need for a posteriori methods of investigations in order to analyze self-organized transformations in biological systems.

Adaptation, Physiological↗
PubMed1998

Effect of environmental complexity on salt-adaptation in Sorghum bicolor.

Individual plants of Sorghum bicolor mature different adaptive reactions to salinity. The positive correlation between the frequency of specific reactions and the tolerance level of plants with that specific reaction results from adaptive determinism (AD). The effect of complexity in environmental conditions on AD in plants adapting for the first time to salinity, A0, and in their offspring A1 was compared. Environmental complexity increases when uncontrolled factors besides salinity affect plants. The diversity of reactions of plants correlates positively with environmental complexity. AD correlates negatively to environmental complexity in A1. In contrast, environmental complexity has no effect on AD in A0. A1 are more tolerant to salinity than A0: A1 inherited adaptive information. The likelihood that A1 plants are exposed to the same uncontrolled factor as their parent is low, and so the decrease of AD in A1 due to environmental complexity suggests that: (1) the information inherited is specific to saline conditions; (2) AD results mainly from inherited pre-existing information; plants react as informatively closed systems: saline conditions trigger the expression of an adaptive, pre-existing program. For A0, conclusions are: (1) the adaptive reaction is not an expression of pre-existing information; (2) AD emerges during the adaptation process: the environment imprints adaptively the plant's development. In this case, plants react as open systems in terms of information.

Adaptation, Physiological↗
PubMed1981

Microbial Changes in Sweet Sorghum (Sorghum bicolor) Juices.

Juice freshly expressed from Sorghum bicolor for making sweet sorghum syrup contained 10 microorganisms per ml. The dominant bacterium was Leuconostoc mesenteroides, followed by gram-negative rods. Lactobacilli, yeasts, and nonfecal coliform bacteria each comprised about 1% of the microbial population. Spoilage of juice, manifested by a sour odor, discoloration, and foaming, occurred between 5 and 12 h at ambient temperatures. Spoilage was correlated with a drop in pH from 4.9 to 4.5 L. mesenteroides was the dominant spoiling agent at 20 degrees C, and Lactobacillus plantarum was the dominant spoiling agent at 32 degrees C, as determined by pure culture studies. Juice may be stored for 14 days at 4 degrees C if promptly refrigerated.

Journal Article↗
PubMed1994

Chemically Induced Cuticle Mutation Affecting Epidermal Conductance to Water Vapor and Disease Susceptibility in Sorghum bicolor (L.) Moench.

Analysis of Sorghum bicolor bloomless (bm) mutants with altered epicuticular wax (EW) structure uncovered a mutation affecting both EW and cuticle deposition. The cuticle of mutant bm-22 was about 60% thinner and approximately one-fifth the weight of the wild-type parent P954035 (WT-P954035) cuticles. Reduced cuticle deposition was associated with increased epidermal conductance to water vapor. The reduction in EW and cuticle deposition increased susceptibility to the fungal pathogen Exserohilum turcicum. Evidence suggests that this recessive mutation occurs at a single locus with pleiotropic effects. The independently occurring gene mutations of bm-2, bm-6, bm-22, and bm-33 are allelic. These chemically induced mutants had essentially identical EW structure, water loss, and cuticle deposition. Furthermore, 138 F2 plants from a bm-22 x WT-P954035 backcross showed no recombination of these traits. This unique mutation in a near-isogenic background provides a useful biological system to examine plant cuticle biosynthesis, physiology, and function.

Journal Article↗
PubMed2002

Genetic mapping of QTLs associated with greenbug resistance and tolerance in Sorghum bicolor.

Ninety three recombinant inbreds of Sorghum bicolor (L. Moench) were derived from a cross between two sorghum lines GBIK and Redlan. This population was used to identify quantitative trait loci (QTLs) for resistance and tolerance to greenbug ( Schizaphids graminum Rondani) Biotypes I and K. One hundred and thirteen loci (38 SSRs and 75 RAPDs) were mapped in 12 linkage groups covering 1,530 cM. In general, nine QTLs were detected affecting both resistance and tolerance to greenbug (GB) Biotypes I and K. The phenotypic variance explained by each QTL ranged from 5.6% to 38.4%. Four SSRs and one RAPD marker were associated with the expression of all resistance and tolerance traits. These markers appear to be linked to biotype non-specific resistance and tolerance genes. Four additional markers were associated with biotype-specific resistance or tolerance traits. The detection of more than one locus for each biotype supports the hypothesis that several regions, which represent different genes, control the expression of resistance and tolerance to greenbug in sorghum. The results can be used for marker-assisted selection and the breeding of greenbug-tolerant sorghum cultivars.

Journal Article↗
PubMed1995

Genetic Regulation of Development in Sorghum bicolor (X. Greatly Attenuated Photoperiod Sensitivity in a Phytochrome-Deficient Sorghum Possessing a Biological Clock but Lacking a Red Light-High Irradiance Response).

The role of a light-stable, 123-kD phytochrome in the biological clock, in photoperiodic flowering and shoot growth in extended photoperiods, and in the red light-high irradiance response was studied in Sorghum bicolor using a phytochrome-deficient mutant, 58M (ma3R ma3R), and a near-isogenic wild-type cultivar, 100M (Ma3 Ma3). Since chlorophyll a/b-binding protein mRNA and ribulose bisphosphate carboxylase small subunit mRNA cycled in a circadian fashion in both 58M and 100M grown in constant light, the 123-kD phytochrome absent from 58M does not appear necessary for expression or entrainment of a functional biological clock. Although 58M previously appeared photoperiod insensitive in 12-h photoperiods, extending the photoperiod up to 24 h delayed floral initiation for up to 2 weeks but did not much affect shoot elongation. Thus, although 58M flowers early in intermediate photoperiods, a residual photoperiod sensitivity remains that presumably is not due to the missing 123-kD phytochrome. Since rapid shoot elongation persists in 58M under extended photoperiods despite delayed floral initiation, long photoperiods uncouple those processes. The observed absence of a red light-high irradiance response in 58M, in contrast to the presence of the response in 100M, strengthens the suggestion that the 123-kD phytochrome missing from 58M is a phyB.

Journal Article↗
PubMed1975

The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor.

A microsomal fraction from seedlings of Sorghum bicolor (Linn) Moench has been shown to catalyze the conversion of L-tyrosine to p-hydroxymandelonitrile via p-hydroxyphenylacetaldoxime. This transformation is consistent with the general pathway for cyanogenic glycoside biosynthesis proposed on the basis of in vivo experiments. When the microsomal fraction was combined with a protein fraction from the soluble portion of the cell and uridine diphosphate glucose, it was possible to demonstrate the synthesis of the cyanogenic glycoside dhurrin from L-tyrosine.

Aerobiosis↗
PubMed2026

Genome-Wide Identification and Expression Pattern of the ANK Gene Family in Sorghum bicolor Under Salt Stress.

The Ankyrin-repeat proteins (ANKs) play a key role in plant development and in response to abiotic stress. This research identified family members of the ANK genes in Sorghum bicolor at the whole-genome level, analyzed their sequence characteristics, evolutionary relationships, and expression patterns, and provided a scientific basis for elucidating the functionality of SbANK genes and for salt-tolerant breeding. Using bioinformatics methods, this study conducted a comprehensive identification of the SbANK gene family, analyzing its physicochemical properties, domain composition, chromosomal distribution, colinearity relationships, promoter cis-acting elements, and conserved protein motifs. Transcriptomic data and qRT-PCR were used to detect changes in their expression under salt stress. A total of 186 ANK family members were identified in the Sorghum bicolor genome, classified into 13 subfamilies and unevenly distributed across 10 chromosomes. Intra-species colinearity analysis revealed 7 pairs of duplicated genes, while inter-species colinearity analysis showed that S. bicolor and Oryza sativa share 88 pairs of orthologs, far exceeding the number found in Arabidopsis thaliana (11 pairs). Promoter analysis indicated that SbANK genes are enriched with cis-acting elements associated with hormone responses (particularly MeJA elements, accounting for 51.7%) and stress responses (particularly anaerobic-inducible elements, accounting for 60.9%). Transcriptomic expression analysis revealed that SbANK genes exhibit distinct tissue specificity, with the ANK-IQ subfamily highly expressed in leaves and the ANK-M subfamily showing the most widespread response under salt stress. Expression levels of the 10 candidate genes showing the most significant responses to salt stress were analyzed using qRT-PCR. The results indicated that SbANK91, SbANK135, and SbANK136 were significantly upregulated under 200 mmol/L NaCl treatment. The SbANK family is distinguished by a large number of member genes and structural diversity, with the ANK-M subfamily being the primary group responding to salt stress. SbANK91, SbANK135, and SbANK136 are identified as putative candidate genes for salt stress responses.

Sorghum↗
PubMed2006

Collagen and muscle stain obtained from Sorghum bicolor.

Crude ethanolic extracts of the leaves of Sorghum bicolor L. Moench were used as stains for tissue sections. The alkaline mixtures did not stain any of the tissues used but the acidic and neutral alcoholic mixtures stained collagen fibres, muscles and red blood cells in shades of pinkish-yellow. Good contrast was obtained when nuclei were pre-stained with Weigert's haematoxylin. The ethanolic extract of S. bicolor was run through a column using silica gel (60-120 mesh) as the stationary phase and various dilutions of hexane, chloroform and ethanol as the mobile phase. Eight major fractions monitored with thin-layer chromatography were obtained but the staining compound was contained in the reddish-brown compound. Re-chromatography of this fraction showed a pure dye, which was used as a stain and subsequently eluted from the sections. Phytochemical screening of the eluted compound revealed that this is a flavonoid, previously described as apigeninidin, (3-deoxypelargonidin).

Anthocyanins↗
PubMed2005

Molecular tagging of gene conferring leaf blight resistance using microsatellites in sorghum [Sorghum bicolor (L.) Moench].

Resistance to leaf blight in sorghum [Sorghum bicolor (L.) Moench] accession G-118 was found to segregate as a single dominant trait in a cross to susceptible cultivar, HC-136. Molecular marker(s) linked to the locus for disease resistance was identified using simple sequence repeat (SSR) markers coupled with bulk segregant analysis. Genomic DNA from the parental cultivars and bulks were screened by PCR amplification with 50 simple sequence repeat primer pairs. Out of these, 38 SSR primers produced polymorphism between parents. After screening of these 38 SSRs with resistant and susceptible bulk, one SSR primer, Xtxp 309 produced a unique band of approximately 700 bp only in resistant parent and resistant bulk and a unique band of 450 bp only in susceptible parent and susceptible bulk. Upon screening with individual resistant and susceptible recombinant inbred lines (RILs), marker Xtxp 309 produced amplification in 23 of the 26 resistant RILs and no amplification was produced in any of the 25 susceptible RILs. The same marker Xtxp 309 produced amplification in 21 of the susceptible RILs and 3 of the resistant RILs of 450 bp band. This was found to be located at a distance of 3.12 cM away from the locus governing resistance to leaf blight which was considered to be closely linked and 7.95 cM away from the locus governing susceptibility to leaf blight. This marker may prove useful in MAS for gene introgression, plant genetic diagnostics and gene pyramiding for resistance via genetic transformation for disease resistance in plants.

Fungi↗
PubMed1981

Phloem Transport and the Regulation of Growth of Sorghum bicolor (Moench) at Low Temperature.

Leaf expansion in Sorghum bicolor (Moench) was severely retarded by low night temperatures (5 C). However, this was not reflected in the early measurements of relative growth rate, indicating that the response was not associated with a deterioration of the photosynthetic system. For plants grown at 30/25 C (day/night) and subsequently held at an ambient temperature of 30 C, phloem transport, as measured either by the movement of (14)C-photosynthate through a zone of controlled temperature or by accumulation of dry matter distal to this zone, was inhibited by temperatures below 10 C. The speed of movement of (32)P through the temperature controlled zone was more sensitive to temperature with reductions apparent below 20 C. Although there was some recovery in the movement of (32)P following 3 days equilibration at low temperature (1 to 10 C), the new values (approximately 100 centimeters per hour) were still only about one-third of those obtained in the high temperature controls. For plants held at an ambient temperature of 21 C, which is well below the optimum for growth, translocation was only inhibited by temperatures below 5 C. Although low temperature may reduce the carrying capacity of the phloem of S. bicolor, this is unlikely to be an important factor in regulating the growth of the plants at low temperatures.

Journal Article↗
PubMed1998

Physical mapping of the liguleless linkage group in Sorghum bicolor using rice RFLP-selected sorghum BACs.

Physical mapping of BACs by fluorescent in situ hybridization (FISH) was used to analyze the liguleless (lg-1) linkage group in sorghum and compare it to the conserved region in rice and maize. Six liguleless-associated rice restriction fragment length polymorphism (RFLP) markers were used to select 16 homeologous sorghum BACs, which were in turn used to physically map the liguleless linkage group in sorghum. Results show a basic conservation of the liguleless region in sorghum relative to the linkage map of rice. One marker which is distal in rice is more medial in sorghum, and another marker which is found within the linkage group in rice is on a different chromosome in sorghum. BACs associated with linkage group I hybridize to chromosome It, which was identified by using FISH in a sorghum cytogenetic stock trisomic for chromosome I (denoted It), and a BAC associated with linkage group E hybridized to an unidentified chromosome. Selected BACs, representing RFLP loci, were end-cloned for RFLP mapping, and the relative linkage order of these clones was in full agreement with the physical data. Similarities in locus order and the association of RFLP-selected BAC markers with two different chromosomes were found to exist between the linkage map of the liguleless region in maize and the physical map of the liguleless region in sorghum.

Basic-Leucine Zipper Transcription Factors↗
PubMed2006

Functional characterization of desaturases involved in the formation of the terminal double bond of an unusual 16:3Delta(9,12,150) fatty acid isolated from Sorghum bicolor root hairs.

Sorgoleone, produced in root hair cells of sorghum (Sorghum bicolor), is likely responsible for much of the allelopathic properties of sorghum root exudates against broadleaf and grass weeds. Previous studies suggest that the biosynthetic pathway of this compound initiates with the synthesis of an unusual 16:3 fatty acid possessing a terminal double bond. The corresponding fatty acyl-CoA serves as a starter unit for polyketide synthases, resulting in the formation of 5-pentadecatrienyl resorcinol. This resorcinolic intermediate is then methylated by an S-adenosylmethionine-dependent O-methyltransferase and subsequently dihydroxylated, yielding the reduced (hydroquinone) form of sorgoleone. To characterize the corresponding enzymes responsible for the biosynthesis of the 16:3 fatty acyl-CoA precursor, we identified and cloned three putative fatty acid desaturases, designated SbDES1, SbDES2, and SbDES3, from an expressed sequence tag (EST) data base prepared from isolated root hairs. Quantitative real-time RT-PCR analyses revealed that these three genes were preferentially expressed in sorghum root hairs where the 16:2 and 16:3 fatty acids were exclusively localized. Heterologous expression of the cDNAs in Saccharomyces cerevisiae revealed that recombinant SbDES2 converted palmitoleic acid (16:1Delta(9)) to hexadecadienoic acid (16:2Delta(9,12)), and that recombinant SbDES3 was capable of converting hexadecadienoic acid into hexadecatrienoic acid (16:3Delta(9,12,15)). Unlike other desaturases reported to date, the double bond introduced by SbDES3 occurred between carbons 15 and 16 resulting in a terminal double bond aliphatic chain. Collectively, the present results strongly suggest that these fatty acid desaturases represent key enzymes involved in the biosynthesis of the allelochemical sorgoleone.

Amino Acid Sequence↗
PubMed2003

Antioxidant constituents from the stem of Sorghum bicolor.

The EtOAc soluble fraction from the stem of Sorghum bicolor showed a strong free radical scavenging activity. Five major compounds were isolated from this fraction. They were identified by spectral data as methyl ferulate (1), methyl p-hydroxycinnamate (2), p-hydroxybenzaldehyde (3), tricin (4), and quercetin 3,4'-dimethyl ether (5). Among these compounds, 1 exhibited a strong, free radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) with an IC50 value of 0.7 microM. We further studied the effects of these isolated compounds on the lipid peroxidation in rat liver microsomes induced by non-enzymatic method. All five compounds showed anti-lipid peroxidation activity (IC50 values of 0.5, 0.4, 0.3 and 0.3 microM, respectively).

Animals↗
PubMed2000

Leaf sheath cuticular waxes on bloomless and sparse-bloom mutants of Sorghum bicolor.

Leaf sheath cuticular waxes on wild-type Sorghum bicolor were approximately 96% free fatty acids, with the C28 and C30 acids being 77 and 20% of these acids, respectively. Twelve mutants with markedly reduced wax load were characterized for chemical composition. In all of the 12 mutants, reduction in the amount of C28 and C30 acids accounted for essentially all of the reduction in total wax load relative to wildtype. The bm2 mutation caused a 99% reduction in total waxes. The bm4, bm5, bm6, bm7 and h10 mutations caused more than 91% reduction in total waxes, whereas the remaining six mutants, bm9, bm11, h7, h11, h12 and h13, caused between 35 and 78% reduction in total wax load. Relative to wild-type, bm4 caused a large increase in the absolute amount of C22, C24 and C26 acids, and reduction in the C28 and longer acids, suggesting that bm4 may suppress elongation of C26, acyl-CoA primarily. The h10 mutation increased the absolute amounts of the longest chain length acids, but reduced shorter acids, suggesting that h10 may suppress termination of acyl-CoA elongation. The bm6, bm9, bm11, h7, h11, h12 and h13 mutations increased the relative amounts, but not absolute amounts, of longer chain acids. Based on chemical composition alone, it is still uncertain which genes and their products were altered by these mutations. Nevertheless, these Sorghum cuticular wax mutants should provide a valuable resource for future studies to elucidate gene involvement in the biosynthesis of cuticular waxes, in particular, the very-long-chain fatty acids.

Acetyl Coenzyme A↗
PubMed2005

Perturbed reproductive development in salt-treated Sorghum bicolor: a consequence of modifications in regulation networks?

In Sorghum bicolor, tolerance to salinity is improved by a 3-week treatment with 150 mM NaCl during early vegetative development. However, a strong decrease in fertility is also observed, suggesting that reproductive development becomes perturbed by this adaptive response to salinity. This study is an attempt to clarify the origin of such a paradoxical phenomenon. The relationships between end-cycle characters are modified by the NaCl treatment: some linkages disappear, while others are strengthened, especially those linking fertility with plant height. In parallel, a transient reduced level of linkage between leaf characters is observed around the unfolding of the eighth to the tenth leaves, defining a critical period in vegetative development separating two discrete phases. A relationship is observed between events occurring during this short critical period and the NaCl-induced perturbations in fertility. This suggests that reproductive development is conditioned by the influence of salinity on events occurring during a short period of vegetative development, independently of the level of tolerance to salinity quantified by the rate of vegetative growth.

Adaptation, Physiological↗