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At least 19 recordsLinked to original sources

Argyria: clinical implications of exposure to silver nitrate and silver oxide.

This article reports the clinical findings in a work force of 30 individuals who were exposed to silver nitrate and silver oxide. Six individuals had argyria and 20 had argyrosis (deposition of silver in the eye). Measurements of blood silver levels were included as part of the examination. The results of this examination generally support the benign nature of argyria, although the question of silver causing a decrement in kidney function and night vision is not settled. Periodic slit lamp examinations as well as monitoring of silver air concentrations are necessary to assure that engineering controls are actually limiting worker exposure to silver.

Adult

A modified method for the differential staining of spermatozoa using alcoholic acidic silver nitrate.

Differential silver staining patterns have been demonstrated in mammalian spermatozoa, using an aqueous silver nitrate reagent. In the present study, mouse, rabbit, and human spermatozoa were stained using a modification of the earlier method. In the modified method, an alcoholic acidic silver nitrate stain, with subsequent differentiation in alcoholic ammonia, was used. This method enhanced the intensity of staining of the head, mid-piece, and tail. In particular, marked differentiation of the acrosomal, subacrosomal, and postacrosomal regions was obtained, which facilitated determination of acrosomal integrity. Moreover, background interference was reduced, yielding better clarity of the stained smears. The staining was carried out in the cold. This modified technique offers an advantage in the assessment of sperm morphological anomalies and membrane and acrosomal integrity, and is a simple, reliable, and useful method for the evaluation of sperm function.

Acrosome

The colors of silver with silver nitrate staining in dental materials.

Silver nitrate has been used as a stain to study the permeability, degradation, and marginal integrity of dental composite restorations. The reagent results in various colors in the composite or marginal interface. So that the cause of these colors could be investigated, this study compared the appearances of the stained layers by light and electron microscopy. The results indicated that the colors could be explained by the distribution of silver particles ranging from minute colloidal particles (20 nm) in the composite or dentin, to clusters of large particles (0.1-1 micron) in marginal interfaces. The colors can be explained by the absorption and scattering of light by these particles. At elevated temperatures, fused concretions of silver formed in the composite subsurface, resulting in direct reflection of incident light. The colors which appear with silver nitrate staining may indicate the size and distribution of microporosity in the material.

Color

The early development of the human skeleton: a radiographic study of fetuses impregnated with silver nitrate.

The use of a silver nitrate impregnation technique and radiography for delineation of the bones of the developing skeleton is not widely known. Using this method, a study of skeleton development was undertaken over a three-year period and special attention was paid to 20 fetuses under the age of 16 weeks. The dates at which ossification in cartilage occurred and the sequence of bone development were noted. Although the technique may be used to delineate skeletal dysplasias in the stillborn, it is best suited to fetuses aged 9--20 weeks. It may become possible to show the earliest age at which bone dysplasias affect the skeleton though this is speculative at the moment.

Bone Development

Systemic argyria secondary to topical silver nitrate.

A case of generalized argyria secondary to topical silver nitrate use on the oral mucosa is described, and the nature and history of argyria reviewed. The patient has extreme pigmentation of her skin and abdominal viscera, as seen on physical examination, gastroduodenoscopy, and laparotomy. Her use of silver nitrate applicators was uncontrolled by her dentist and pharmacist, and the diagnosis of argyria was not made until 2 1/2 years of heavy usage had passed. Avoidance of similar cases requires the careful scrutiny of silver-containing medicinal usage by all health practitioners and pharmacists. Although the systemic distribution of silver in both elemental and ionic forms can be linked with no systemic toxicity, the resultant pigmentation can be devastating to the patient.

Argyria

Topical chemoprophylaxis with silver sulphadiazine and silver nitrate chlorhexidine creams: emergence of sulphonamide-resistant Gram-negative bacilli.

Controlled trials of 0.5% silver nitrate compresses (SN), 1% silver sulphadiazine cream (SSD), and a cream containing 0.5% silver nitrate and 0.2% chlorhexidine digluconate (SNC) showed that all were comparably effective in protecting burns from infection. SN compresses were much less active against miscellaneous Gram-negative bacilli than the other preparations, and the mean morning and evening temperatures and respiration rates in the patients treated with SN compresses were higher then those of patients treated with SSD. Pseudomonas aeruginosa and Proteus spp, though rare in all groups, were less often found in the patients treated with SN compresses. Sulphonamide-resistant Gram-negative bacilli became predominant during the trial of SSD cream on extensive burns and the prophylactic effectiveness of that preparation was thus reduced in the later stages of the trial.

Administration, Topical

[Silver nitrate burn after Credé's preventive treatment. A roentgen analytic and scanning electron microscopy study].

Following Credé's prophylaxis with silver-nitrate, the cornea of a newborn presented greyish-brown, lime-like plaques on the nasal part of the right eye. A paracentral ulcerating stromal opacification undermined these appositions, when the patient was admitted to the eye-clinic at Aachen. In the material taken in a lamellar keratectomy scanning electron microscopical examination was able to prove the existence of granules, previously described in light-microscopy. These granules measured 100 to 300 nm in diameter and were placed up to 110 microns deep into the corneal stroma of the specimen. An earlier chemical analysis of necrotic material showed no silver specific reaction. By means of EDX-Analysis these granules could be identified as silver-containing. This was once reassured by a newly developed modification of van-Kossa's-staining-method. The fact that the granular deposits contained mainly silver proves that the onset of a sodium-chloride-irrigation did not promote an intended therapeutic silver-chloride-precipitation and therefore had no effects on the silver-nitrate's penetration abilities. Injuries by silver-nitrate-solutions used for Credé's prophylaxis are seldom but still reported. The mechanism of injury in this case of a child, born by sectio remains unknown. Neither the use of an unusual silvernitrate solution, that was taken from a disposable ampoule (Mova-Nitrat) was reported, nor any corneal injury during sectio mentioned.(ABSTRACT TRUNCATED AT 250 WORDS)

Burns, Chemical

A comparison of silver nitrate with erythromycin for prophylaxis against ophthalmia neonatorum.

Although prophylactic eyedrops to treat ophthalmia neonatorum is mandated nationwide, states and hospitals are free to choose specific drugs. To compare two of these agents, we studied the incidence and characteristics of ophthalmia neonatorum in two UCLA teaching hospitals over a five-year period. One, which used 1% silver nitrate solution exclusively, had 50 cases in 34,772 births, a frequency of 0.14%. The other used 0.5% erythromycin ointment exclusively and had 43 cases in 12,652 births, a frequency of 0.34%. Ophthalmia neonatorum was more frequent in the hospital using erythromycin (p less than 0.001), as was chlamydial conjunctivitis (p less than 0.02). Although not statistically significant, gonococcal conjunctivitis was found in four infants, all in the hospital using silver nitrate. Because silver nitrate was found more effective in decreasing the total frequency of all cases of ophthalmia neonatorum, and cases caused by Chlamydia and gram-negative bacteria specifically, this drug still should be considered as a primary prophylactic agent against ophthalmia neonatorum.

Bacteria

Efficacy of ammoniacal silver nitrate in root canal therapy.

In order to find a simple and effective method for endodontic treatment of recalcitrant cases, the efficacy of ammoniacal silver nitrate was re-evaluated. The modified serial tube dilution method was conducted to determine the effective antimicrobial concentration of ammoniacal silver nitrate on selected test microorganisms and clinical isolates. The effective antimicrobial concentration for the most resistant microorganism, Streptococcus faecalis, was 5.0 X 10(-10) g/ml and the effectiveness persisted for several days. The diffusion ability of 4% ammoniacal silver nitrate into the dentinal tubules from root canal wall was also tested with freshly extracted teeth treated as in routine procedure for root canal therapy. The diffusibility was evident and the extent of infiltration was various, with more infiltration at cervical dentin than at apical one.

Ammonia

Anuria following silver nitrate irrigation for intractable bladder hemorrhage.

Intravesical silver nitrate has been used for intractable bladder hemorrhage. A case of anuria resulting from this technique is reported. The etiology of anuria in this patient was probably owing to obstruction from deposition of silver salts. The level of obstruction was the ureterovesical junction on 1 side and the collecting ducts at the renal papillae on the other side. Therapy consisted of vigorous endoscopic evacuation of deposited silver salts from the bladder and hemodialysis with good results.

Adult

Neutralization effect of some agents on the antimicrobial activity of ammoniacal silver nitrate.

The effect of some agents on the antimicrobial activity of ammoniacal silver nitrate, an endodontic medicament, was tested with Streptococcus faecalis by the serial tube dilution method. Its results indicated that sodium hypochlorite, hydrogen peroxide, and blood had a marked inhibitory effect. However, the presence of dentin, necrotic tissue, saliva, and hydrogen sulfide gas liberated from protein decomposition showed no or little effect on the antibacterial properties of the chemical. Since antiseptics or antibiotics generally may be decomposed by necrotic tissues, these findings suggested that the use of ammoniacal silver nitrate not only may resolve the problem of recalcitrant cases in endodontic treatment but also may simplify the disinfecting procedure for root canals.

Animals

Is alcoholic acidic silver nitrate reagent really specific for the histochemical localization of ascorbic acid.

The histochemical localization of ascorbic acid in plant tissues with the alcoholic acidic silver nitrate reagent is shown here to be not specific for ascorbic acid, since some of the polyphenolic substances, including flavonoids, which are known to be widely distributed in plant tissues, are also able to reduce the acidic alcoholic silver nitrate reagent at low temperature (0-4 degrees C) and at pH 2 to 2.5 in dark. This method may perhaps be used for animal tissues where flavonoid pigments do not occur in such large quantities as they do in plants. I therefore, come to the inevitable conclusion that the use of alcoholic acidic silver nitrate reagent in localizing ascorbic acid in plant tissues may be highly misleading.

Ascorbic Acid

Silver nitrate burns following treatment for umbilical granuloma.

Three infants treated for umbilical granuloma with silver nitrate suffered chemical burns to the periumbilical area which prompted visits to the emergency department. Treatment was conservative, and the outcome was good in all cases. We recommend caution when applying silver nitrate to the umbilicus, careful drying of the umbilical exudate to prevent spillage, and discussion with parents that burns may occur but apparently are not serious. The possibility of secondary infection is discussed.

Burns, Chemical

Silver nitrate ophthalmic solution and chemical conjunctivities.

The current status of nursery routines of prophylaxis against ophthalmia neonatorum were surveyed by mail questionnaire to 100 leading maternity hospitals. More than 20% of the respondents were not using silver nitrate, mainly because of chemical conjunctivitis. The clinical significance and incidence of chemical conjunctivitis were studied in 1,000 newborns whose eyes were handled differently. Rinsing after instillation of silver nitrate does not reduce the conjunctival irritation. Although 90% of the infants had conjunctivitis in the first hours of life, the majority cleared within 24 hours. Chemical conjunctivitis did not increase secondary infection, neither did it mask bacterial infection. Silver nitrate is effective in vitro against Neisseria gonorrhoeae and Staphylococcus aureus in a concentration of 0.1% and against Escherichia coli in a concentration of 0.01%.

Conjunctivitis

A comparison between low background silver diammine and silver nitrate protein stains.

Several methods of silver staining of proteins after sodium dodecyl sulfate-electrophoresis in polyacrylamide gels were compared. The most rapid methods were found to be less sensitive than more time-consuming methods. Among the long methods, those using glutaraldehyde treatment of the gel and silver diammine complex as the silvering agent were found to be the most sensitive, at the expense of the use of a modified polyacrylamide matrix and higher silver concentrations.

Ammonia

Prophylaxis of ophthalmia neonatorum: comparison of silver nitrate, tetracycline, erythromycin and no prophylaxis.

From November, 1989, to October, 1991, 4544 neonates were born at our hospital. Neonatal ocular prophylaxis immediately after birth was used with 1% tetracycline ophthalmic ointment in 1156 neonates, 0.5% erythromycin ophthalmic ointment in 1163 neonates and 1% silver nitrate drops in 1082 neonates. No prophylaxis for neonatal conjunctivitis was given to 1143 neonates. A total of 302 infants (6.7%) developed conjunctivitis during the first 4 weeks of life. Between December, 1991, and January, 1992, 425 neonates were born at our hospital and all were given 0.5% erythromycin ophthalmic ointment twice in the first 24 hours after birth for ocular prophylaxis. Thirty-one (7.3%) infants developed conjunctivitis during the neonatal period. The incidence rates of neonatal chlamydial conjuctivitis in the tetracycline, erythromycin, silver nitrate, no prophylaxis and erythromycin twice groups were 1.3, 1.5, 1.7, 1.6 and 1.4%, respectively. We conclude that neonatal ocular prophylaxis with erythromycin (one or two doses) or tetracycline or silver nitrate does not significantly reduce the incidence of neonatal chlamydial conjunctivitis compared with that in those given no prophylaxis.

Conjunctivitis, Bacterial

Treatment of allergic and vasomotor rhinitis by the local application of different concentrations of silver nitrate.

In 1980, Bhargava et al. reported a new treatment of allergic and vasomotor rhinitis by the local application of 15 per cent silver nitrate. The results of further studies of the treatment by using different concentrations of silver nitrate ranging from 5 per cent to 25 per cent and normal saline as placebo are presented here; 15 per cent has been found to be the most effective concentration giving successful results in 75.7 per cent of cases. It is applied to the anterior portion of both inferior turbinates and the anterior part of the nasal septum once a week on five occasions. Best relief was obtained from the most annoying symptoms of sneezing and rhinorrhoea. Asthma was controlled in 50 per cent of such patients who had attacks of allergic rhinitis followed by asthma. For this widely prevalent disease, the treatment was found to be simple, easy and useful, with negligible side-effects.

Administration, Topical

Correspondence of silver nitrate staining patterns in decalcified bone withe the microradiographic image.

A procedure for differential staining of decalcified bone with silver nitrate showed major histological features which appeared to correspond closely to microradiographic images. The extent to which this is actually the case was investigated directly by preparing microradiographs of ground sections of baboon and dog radii and then decalcifying and staining the same sections. The many detailed similarities indicate that this staining procedure is a useful adjunct to microradiography. Thus, poorly mineralized osteons or layers of circumferential lamellae are darker stained by silver nitrate, and the variably mineralized layers of circumferential lamellae are closely duplicated by light and dark bands in the stained sections. These similarities imply that there is a relationship between the mineral density of bone and some condition of the organic matrix which is probably related to maturation changes in the collagen.

Age Factors