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The nutritive value of silages. Digestion of nitrogenous constituents in sheep receiving diets of grass silage and grass silage and barley.

1. Two experiments were conducted to study the digestion of nitrogenous constituents in the rumen, small intestine and caecum and colon of sheep given diets of grass silage or grass silage and barley. Three silages were used. One was made from first-harvest grass in the spring and the other from regrowth grass cut in either early autumn or late autumn. All were of perennial ryegrass (Lolium perenne) and were preserved with formic acid. 2. Expt 1 involved a comparison between the spring silage given alone (644 g dry matter (DM)/d) and the spring silage supplemented with barley (151 g DM/d). The intakes (g/d) of total nitrogen for the silage diet and for the supplemented diet were 14.89 and 17.36. Corresponding values (g/d) for N passage were 15.55 and 18.53 (P < 0.01) and the duodenum, 6.01 and 7.09 at the ileum and 5.06 and 5.52 in the faeces. The barley supplement had no significant (P < 0.05) effect on rumen ammonia-N concentration. 3. Expt 2 involved a comparison between the two autumn-cut silages each offered at a level of feeding of approximately 700 g DM/d. The intakes (g/d) of total N for the early-cut silage and for the late-cut silage were 21.67 and 15.62 respectively. Corresponding values (g/d) for N passage were 17.10 and 16.96 at the duodenum, 6.65 and 6.80 at the ileum and 4.5 and 5.22 in the faeces. The concentration of NH3-N in the rumen was significantly (P < 0.001) higher with the early-cut silage than with the late-cut silage. 4. In both experiments the rates of bacterial crude protein (N x 6.25) synthesis in the rumen, estimated using alpha, epsilon-diaminopimelic acid as a marker, were low, 142 and 161 g crude protein/kg organic matter apparently digested in the rumen for the spring silage and the spring silage and barley diets respectively, and 68 and 103 g crude protein/kg organic matter apparently digested in the rumen for the early-cut autumn silage and the late-cut autumn silage respectively. For all diets there was a relatively low contribution of bacterial crude protein to the duodenal passage of crude protein and the amounts of individual amino acids ingested in the diets had a marked influence on the amino acids passing to the duodenum and as a consequence on the mixture of amino acids taken up from the small intestine. 5. The results are discussed in relation to the nutritive value of silage N for ruminants.

Amino Acids

The nutritive value of silages. Digestion of organic matter, gross energy and carbohydrate constituents in the rumen and intestines of sheep receiving diets of grass silage or grass silage and barley.

1. Two experiments were conducted to study the digestion of organic matter, gross energy and carbohydrate constituents in the rumen, small intestine and caecum and colon of sheep given grass silage diets. Three silages made from perennial ryegrass (Lolium perenne) with formic acid as an additive were used. One was made from first-harvest grass in the spring and the others from regrowth grass cut from a single sward in either early autumn or late autumn. Expt 1 involved a comparison between the spring silage given alone or supplemented with barley (silage:barley, 4:1 dry matter (DM) basis). Expt 2 involved a comparison between the early-cut and late-cut autumn silages. 2. In Expt 1, supplementation of the silage with barley resulted in a non-significant (P > 0.05) reduction in the proportion of digestible energy (DE) and digestible organic matter digested in the rumen and an increase in the proportions digested in the small intestine. There were also pronounced effects of barley on ruminal cellulolysis and the proportion of digestible cellulose broken down in the rumen was reduced (P < 0.05) from 0.90 to 0.77. There was an increased passage of alpha-linked glucose polymers to the duodenum but even with the supplemented diet 0.91 of the dietary polymers were digested in the rumen. The molar proportion of propionic acid in the rumen tended to be reduced and there were increases in the proportions of butyric acid (P < 0.01) and acetic acid. 3. Expt 2, the digestibility of organic matter, gross energy and cellulose in the early-cut silage was higher (P < 0.01) than in the late-cut silage but there were no significant (P < 0.05) differences between silages in sites of digestion of these constituents. However, the molar proportion of acetic acid in the rumen was higher (P < 0.01) and the molar proportion of propionic acid was lower (P < 0.01) with the late-cut silage than the early-cut silage. 4. The results are discussed in relation to the voluntary intake and utilization of high-digestibility silages.

Animal Feed

The nutritive value of silages. Energy metabolism in sheep receiving diets of grass silage or grass silage and barley.

1. Two calorimetric experiments were conducted to study the utilization of energy in sheep given diets of grass silage or grass silage and barley. Three silages were investigated. One was made from first-harvest grass in the spring (S) and the others from regrowth cut either early or late in the autumn (E and L respectively). All were of perennial ryegress (Lolium perenne) and preserved with formic acid. Each silage was given at two levels of feeding, the lower providing approximately a maintenance energy intake. The S and L silages were also given supplemented with barley. 2. The digestibilities of organic matter, cellulose and energy in the silages were high. Measured at maintenance, digestible energy (DE) contents (MJ/kg dry matter (DM))were 11.83, 14.67 and 12.90 for S, E and L respectively. The DE contents of the S and E silages were depressed at the higher level of feeding but the effect was offset by changes in the energy losses as methane and urine. Metabolizable energy (ME) contents (MJ/kg DM) for the three silages, S, E and L were respectively 9.88, 12.54 and 10.73 at the low level of feeding and 9.91, 11.99 and 11.08 at the high level of feeding. The mean ME content of barley calculated by difference was 13.76 MJ/kg DM. 3. The mean efficiencies of utilization of ME for maintenance (km) for the S, E and L silages were 0.69, 0.71 and 0.68 respectively. Corresponding values for fattening (kf) were 0.21, 0.57 and 0.59. Excepting the kf for the S silage which was low, observed efficiencies were in broad agreement with those predicted by the equations of the Agricultural Research Council (1965). Similar agreement was obtained with all diets consisting of silage and barley.

Animal Feed

[Occurrence of Fusarium strains and their mycotoxins in corn silage. 7. Formation of deoxynivalenol (DON) in a silage corn plot artificially inoculated with Fusarium culmorum and the effect of silaging on the stability of the DON formed].

The formation of deoxynivalenol in a maize plot inoculated with Fusarium culmorum was studied over a growing season. Already three weeks after inoculation 4.9 mg/kg of DON were measured in the infected ears. The toxin concentration increased continuously up to harvest after eight weeks to a value of 261 mg/kg. Ensilage experiments in laboratory scale silos have shown that the DON content of naturally contaminated corn-cob-mix was not reduced during the ensilage process. It was concluded that infection of maize plants by toxin-producing Fusarium species followed by DON production in the field seems to be the most probable way of contamination of maize silage with this mycotoxin.

Animals

The effect of formaldehyde or glutaraldehyde application to lucerne before ensiling on silage fermentation and silage N digestion in sheep.

The primary growth of lucerne (Medicago sativa) was ensiled after treatment with either formic acid alone (4.1 litres/t; silage F) or with formic acid and either formaldehyde (30.5 g/kg crude protein (nitrogen X 6.25; CP); silage FF), glutaraldehyde (44.2 g/kg CP; silage FG) or a mixture of the two aldehydes at approximately half their individual application rates (silage FFG). Compared with formic acid alone, both formaldehyde and glutaraldehyde reduced protein breakdown and carbohydrate fermentation during ensiling. The extent of protein protection afforded within the silo was similar for the two aldehydes, whereas formaldehyde was more effective in restricting carbohydrate fermentation. The effect of treatment FFG on silage fermentation was confounded by the silo bag bursting and the development of a clostridial-type fermentation. All aldehyde treatments reduced silage soluble-N content but N disappearance when the silages were incubated in polyester bags in the rumen was high for all silages and reductions due to the aldehydes were small. Silage digestion was studied in four mature sheep each fitted with a rumen cannula and re-entrant cannulas in the proximal duodenum and distal ileum. The apparent digestibility of organic matter (OM) in the whole tract was reduced (P less than 0.05) to a similar extent by both aldehydes, whereas rumen OM digestion was reduced (P less than 0.05) more by glutaraldehyde than by formaldehyde. The effects on digestion appeared to be due to the action of the aldehydes on the foods rather than to any adverse influences of the aldehydes on the metabolism of the rumen microbes because, although rumen ammonia levels were lower (P less than 0.05) when the aldehyde-treated silages were given, rumen casein-degrading activity, the degradation of different feedstuffs when incubated in polyester bags in the rumen and microbial N flow at the duodenum did not differ (P greater than 0.05) between silages. All aldehyde treatments decreased (P less than 0.05) the apparent digestibility of N in the whole tract. Silage N degradability in the rumen was also decreased (P less than 0.05) from 0.82 for silage F to 0.67, 0.60 and 0.62 for silages FF, FG and FFG respectively, and consequently non-ammonia-N (NAN) flow at the duodenum increased (P less than 0.05). The aldehydes did not adversely affect the apparent digestibility of NAN in the small intestine, and net NAN absorption from the small intestine increased from 8.8 g/d with silage F to 11.4, 15.3 and 14.2 g/d with silages FF, FG and FFG respectively.(ABSTRACT TRUNCATED AT 400 WORDS)

Aldehydes

Evaluation of an alfalfa silage-corn silage roughage feeding program for raising dairy heifers.

One hundred and ninety-seven dairy heifers, which included a Holstein line, an Ayrshire line, and a reciprocal cross line, were assigned randomly to two forage treatments at 57 days of age. Forages fed to control heifers consisted of 40% grass-legume hay, 20% wilted grass silage, and 40% corn silage (dry matter). The second, or silage group, was fed a dry matter mixture of 40% corn silage and 60% direct cut alfalfa silage, formic acid treated. Both groups of heifers were fed the same quantity of calf starter and calf grower rations. In general, heifer weight and size were greater with the all-silage forage. At 350 and 574 days of age, heifers on the all-silage ration were heavier than their controls. Heifers fed the all-silage ration had greater heart girths and wither heights. Reproductive performance, as measured by the number of services per conception, was not significantly different in the two treatment group. There were no health problems that could be attributed directly to dietary treatment. Dairy heifers were reared successfully on a forage mixture of corn silage and formic acid treated alfalfa silage when additional concentrate was fed during the 1st yr.

Animal Feed

Effects of silage pH on voluntary intake of corn silage.

We evaluated effects of silage pH on corn silage intake. Sixteen Holstein heifers (292 kg) were fed control corn silage during a 2-wk preliminary period. This was followed by an 8-wk experimental period in which animals were fed silage neutralized with 0, 2, 4, or 6% sodium bicarbonate (dry matter) added prior to feeding with corresponding pH's 3.72, 4.46, 5.62, and 8.05. Organic matter intake was increased 1.0 and 1.2 kg/day by addition of 2 and 4% sodium bicarbonate versus controls whereas 6% sodium bicarbonate reduced intake .7 kg/day. An equation developed to predict organic matter intake from silage pH was: Y = 3.20 + 3.92 (pH) -.35 (pH)2 with coefficient of determination .66. This equation predicted maximum organic matter intake at pH 5.6. It appears that silage pH is a factor that affects voluntary consumption of corn silage and that pH 5 to 6 is optimum, whereas silage pH above and below may reduce intake.

Animal Feed

Biogenic amines in silage. 3. The occurrence of six biogenic amines in farm-scale grass and maize silages.

Concentrations of putrescine (PUT), cadaverine (CAD), spermidine (SPD), spermine (SPM), histamine (HIS) and tyramine (TYR) in 53 grass silages and 54 maize silages were determined using an HPLC method. Concentrations of all amines excluding SPD in grass silages decreased significantly with increasing dry matter (DM) contents and decreasing acetic acid concentrations. The mean concentrations were 1310, 642, 414, 139, 120 and 33.6 mg/kg for TYR, CAD, PUT, SPM, HIS and SPD respectively in grass silages of 20-30% DM. The maximal values found were twice to five times higher than the mean values. The effect of increasing DM on the amines concentrations was not evident in maize silages. The mean concentrations, regardless of the DM content, were surprisingly high: 435, 388, 341, 71.7, 25.1 and 5.4 mg/kg for TYR, PUT, CAD, HIS, SPD and SPM. The maximal values in maize silages were three times to ten times higher than the mean values. Considering that maize silage forms the great proportion of the diets of cattle during long-term winter feeding, further toxicological and physiological research should be carried out.

Animals

Inhibition of proteolysis at harvest using heat in alfalfa silages: effects on silage composition and digestion by sheep.

Heat treatment at harvest was used to investigate the effects of proteolysis on silage composition and digestion by sheep. Four alfalfa (Medicago sativa) silages were prepared, two from mid-bloom and two from pre-bloom crops from the same field. Mid-bloom alfalfa was conserved with formic acid as two unwilted silages, either without (unwilted control; UWC) or after heat treatment (unwilted heated; UWH) applied as steam for 1 min. Pre-bloom alfalfa was ensiled either after 24 h wilting (wilted control; WC) or after heating in a crop dehydrator for 2 min (wilted control; WH). Heated treatments were inoculated with Lactobacillus plantarum. Eight wethers, cannulated in the rumen and duodenum, were given the silages to determine the effects of heat treatment on digestion. Heat treatment inhibited protease activity and reduced protein catabolism in the silo. In unwilted silages, heat treatment had no effects (P greater than .05) on OM or N digestion, but it reduced (P less than .05) CP degradability in the rumen. In wilted silages, heat treatment reduced (P less than .05) apparent OM digestion in the rumen and increased (P less than .05) the proportion of N intake flowing to the intestines as non-ammonia N (NAN). Efficiency of microbial protein synthesis also was increased (P less than .01). Absorption of N posterior to the duodenum was increased (P less than .05) in WH compared to WC, but there was no effect (P greater than .05) of heat treatment on apparent total tract N digestibility.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed

Sodium bicarbonate for early lactation cows fed corn silage or hay crop silage-based diets.

To compare the effects of NaHCO3 in diets based on different forage sources, 16 Holstein cows, in a split-plot design, were assigned at 2 d postpartum to a total mixed diet of either 30% hay crop silage: 70% concentrate or 40% corn silage: 60% concentrate (dry basis) that contained 0 or 1.25% NaHCO3. Over the first 6 wk postpartum, NaHCO3 increased milk fat percentage in cows fed the corn silage-based diet. During wk 2 through 6 postpartum, NaHCO3 increased milk yield with the hay crop silage-based diet and tended to decrease milk yield with the corn silage-based diet. Sodium bicarbonate increased digestion of NDF with both forages and increased excretion of urinary nitrogen with the corn silage-based diet. Responses to NaHCO3 by cows in early lactation may depend on the nature of the dietary forage component.

Animal Feed

Effects of cereal grain silages versus alfalfa silage on chewing activity and reticular motility in early lactation cows.

Four early lactation Holstein cows fitted with ruminal and duodenal cannulas were used in a 4 x 4 Latin square design experiment to determine the effects of whole crop barley, oat, and triticale silages substituted for alfalfa silage in the forage portion of diets on chewing behavior and the relationships between attributes of reticular contractions and voluntary intake and digesta passage from the reticulorumen. Mean ad libitum DMI was 18.3 kg/d and was affected by diet. Duodenal flows of DM, NDF, and OM were not affected by diet. The frequency and duration of reticular contractions during eating, rumination, and resting were not influenced by diet. Amplitude of reticular contractions during eating was lower for cows fed alfalfa than for those fed barley silage. The NDF of alfalfa was transferred less efficiently than that of cereal silages at the duodenum. Cows fed alfalfa and barley silage diets transferred less NDF per unit of duration than cows fed oat and triticale silage diets. However, an equal amount of NDF was transferred per unit of amplitude of contraction, regardless of diet. Changes in DMI and digesta passage could be explained only partially by changes in chewing activity and attributes of reticular contractions required to transfer each unit of digesta from the reticulorumen.

Animal Feed

Group-fed concentrate-silage blend versus individually-fed concentrates and group-fed silage for lactating cows.

System A provided 7.26 kg concentrate per cow daily blended and group fed with silage in 125 total and 97 selected 305-day lactations. System B provided concentrate hand-fed according to each cow's daily production (1 kg concentrate/3.25 kg milk) separate from group-fed silage in 112 total and 75 selected 305-day lactations. The 305-day records excluded those containing more than 150 days open. Both groups had, when available, 2.27 kg hay/cow daily or limited and equal grazing. Combined refusals, 5 to 10% of concentrate-silage blend in A and 5 to 10% of silage in B, plus fresh silage and 2.27 kg concentrate/cow daily constituted the common dry cow ration. Milk, milk fat percentage, and 4% fat-corrected milk, respectively, were 6,475 kg, 3.80%, and 6,270 kg in A and 6,899 kg, 3.69%, and 6,566 kg in B in the total lactations and 5,900 kg, 3.78%, and 5,686 kg in A and 6,098 kg, 3.70%, and 5,818 kg in B in the selected 305-day lactations. Percentages of solids-not-fat and protein in the milk were similar between A and B. Excluding grazing inputs, fat-corrected milk per total digestible nutrients per total lactation was 1.73 kg in both systems. Days open, days in milk, mean Wisconsin Mastitis Test score, body weight, net weight change, days to peak daily milk yield, days to minimum body weight, and minimum body weight were not different between systems in either the total or the selected 305-day lactations. Involuntary culling and culling for low production terminated 18 and 17% of the lactations started on Systems A and B.

Animals

[The effect of feeding corn silage juices on selected indicators of metabolism in dairy cows given feed containing various proportions of silage and hay].

In farm production conditions two comparative trials lasting 58 and 128 days were carried out. Acidogenic exposure to acids of silage juices, silage and haylage (lactic acid, acetic and butyric acids) in feed ration ranged from 0.33 to 2.85 mol per 100 kg live weight. It was proved that it was possible to include 9 to 10 litres of silage juices in feed ration under the condition that the total daily intake would not exceed 1.94 mol of acids per 100 kg live weight. This supplement of silage juices increased milk production. Higher amount of acid (2.65 and 2.85 mol) resulted in acidogenic and ketogenic type of silage juice enriched feed ration, which caused a more rapid decrease of lactation curve in dairy cows fed this feed ration than in those in the control group.

Acids

Effect of ratio of corn silage to grass-legume silage with high concentrate during dry period on milk production and health of dairy cows.

Four corn silage: gross legume silage: concentrate totally mixed diets 1) 75:25:0; 2) 48.75:16.25:35; 3) 50:50:0; and 4) 32.5:32.5:5.35 were fed to Holsteins in second and third lactation for two complete lactations. Cows fed diets 1 or 2 during dry period were fed diet 2 during lactation. Diets did not affect length of dry period or lactation, calf weight, milk protein percent, or milk fat percent. Feeding concentrate during dry period increased gain from .63 to 1.11 kg/day. Feeding higher grass-legume silage diets 3 and 4 increased gain during lactation from .25 to .35 kg/day. On a mature equivalent basis, cows fed high corn silage diets 1 and 2 produced more milk (7,105 versus 6,663 kg), protein (236 versus 216 kg), fat (267 versus 245 kg), solids-not-fat (612 versus 564 kg), and a higher solids-not-fat percent (8.62 versus 8.50%) than diets 3 and 4. Diets did not alter health or reproduction. Production and weight gains favored feeding diet 1 during dry period and diet 2 during lactation.

Animal Feed

Factors affecting the microbial and chemical composition of silage. IV. Effect of wilting on maize silage.

The effect of wilting on the microbial and chemical composition of ensiled maize plants was studied. Wilting stimulated high densities of lactic acid bacteria, with the decrease in counts of undesirable flora, i.e., yeasts, moulds, proteolytic and saccharolytic anaerobes, causing spoilage of silage. Moreover, wilting decreased the losses of dry matter, total acidity, and butyric acid content of silage. Accordingly, wilting proved to be a favourable treatment for the production of good quality silage from maize plants.

Acids

Adrenal response during periparturient period to adrenocorticotropin in dairy cattle fed corn silage and grass legume silage.

Glucocorticoid response to exogenous adrenocorticotropin was used as an indicator of stress in cows fed different diets ad libitum during the dry period and throughout lactation. Twenty-three Holstein cows were administered adrenocorticotropin at 2 d after initiation of the dry period and at 2 d and 35 d postpartum, and plasma glucocorticoid response was evaluated. Cows were fed diets using only corn silage as forage or mixed forage with or without supplemental concentrate. Mean basal plasma glucocorticoid concentrations were 6.1 ng/ml on d 2 after dry off, 4.7 ng/ml on d 2 postpartum, and 6.7 ng/ml on d 35. Cows on the 32.5:32.5:35 corn silage:grass-legume silage:concentrate diet had the lowest basal glucocorticoid concentrations on d 2 postpartum but highest concentrations on d 35 of lactation. Mean glucocorticoid response was 33.2 ng/ml on d 2 of lactation compared with 50.6 ng/ml on d 35 and 48.5 ng/ml plasma on d 2 following drying off. Diet interactions suggest that feed components may be involved with ability to tolerate stresses that occur during lactation and the dry period and thus may lead to altered adrenal function.

Adrenocorticotropic Hormone

Feeding patterns of lactating cows of three different breeds fed hay, corn silage, and grass silage.

Feeding behavior of 35 lactating cows of three different breeds (Holstein-Friesian = HF, Simmental = SI, and Jersey = JER) was compared in the present study. The cows were kept in a loose housing system and fed hay, corn silage (CS) and grass silage (GS) ad lib. Within 7 consecutive days, 2918 meals were recorded and analyzed: 2503 meals were eaten during the light phase (0430-2200), and 57% of these diurnal meals consisted of only one feedstuff (43% hay, 9% CS, 5% GS). During the dark phase, only 23% of the 415 meals consisted of hay alone, but 16% of CS and 21% of GS alone. This circadian distribution showed no breed differences, yet meal patterns did: HF and SI cows ate 11 meals per day, and JER cows ate more than 13 meals/day. Meal size was 9.8MJ, 7.9MJ, and 5.8MJ for HF, SI, and JER cows, respectively. HF cows had the highest 24 h energy intake and JER cows the lowest, but the latter spent the most time feeding. During the light phase all three breeds obtained the most energy from hay, followed by CS and GS. In contrast, during the dark phase most energy was derived from GS, and hay contributed the least. A significant premeal correlation was observed for cows of all breeds, in particular during the time with the highest feeding activity (0800-1300). During the dark phase no premeal correlation could be detected; instead a significant postmeal correlation appeared, in particular in HF and SI cows.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed

Factors affecting the microbial and chemical composition of silage. III. Effect of urea additions on maize silage.

The effect of urea additions on the microbiological and chemical properties of silage, produced from young maize plants (Darawa stage), was studied. Urea treatments, i.e., 0.25%, 0.50%, 0.75%, and 1.00%, stimulated higher densities of the desired microorganisms than the control, while undesired organisms showed lower counts (proteolytic and saccharolytic anaerobes). Addition of 0.25 to 0.50% or urea resulted in the production of high quality silage with pleasant small and high nutritive value, as confirmed by the various microbiological and chemical analyses conducted. Higher levels (0.75 and 1.00%) of urea decreased the quality of the product.

Animal Feed