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Results for “SPERMATOZOA/chemistry”

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At least 19 recordsLinked to original sources
PubMed1961

Decrease in nuclear Feulgen-positive material (DNA) upon aging in in vitro storage of bovine spermatozoa.

The Feulgen-DNA content of sperm cells from 5 bulls was studied by means of microspectrophotometry after storage at 5 degrees C for 2, 3, 5, and 10 days in a yolk-citrate diluent permitting slow aerobic metabolism. A subsample of sperm cells from each bull was subjected to the Feulgen technique on each of the storage days selected. The cells sampled on each of these days received a standard 12 minute, 60 degrees C hydrolysis. Absorption measurements at 546 mmicroof the individual cells indicated a marked progressive decrease in the Feulgen-DNA content of the stored spermatozoa. The loss of 30 per cent of the initial DNA at the end of 5 days' storage was highly significant statistically. This decrease approximately parallels the known decrease in fertility of stored sperm cells, as well as the increase in apparent embryonic mortality resulting from the use of similarly aged spermatozoa for artificial insemination.

Animals↗
PubMed1959

A quantitative estimation of the uptake of two new electron stains by the cytoplasmic membrane of ram sperm.

From microdensitometer measurements on electron micrographs of sectioned sperm heads it has been found that the electron stains, triiodobenzoyl chloride, and triiodophenylisocyanate, increase the image contrast of the cell membrane above its immediate background by about 40 per cent and 70 per cent respectively, while the nucleus remains unstained. Assumptions based on current electron scattering theory have been used to deduce the uptake by weight of the stains in terms of the density of the nucleus, which was estimated from complementary measurements made with the interference microscope and electron microscope. The uptake of the stains was found to be about 7 per cent and 12 per cent by weight respectively. It is suggested that the method used in this work could be applied generally for the density measurement of cell structures unresolved by the light microscope.

Animals↗