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At least 19 recordsLinked to original sources
PubMed1991

Number of beta-receptors in rhinitic pollinic patients.

The study was carried out on venous blood from 67 patients with seasonal rhinoconjunctivitis caused by sensitization to grass pollen and 30 control individuals. Total IgE determination, antigen-specific histamine release test against two concentrations of Phleum pratense, and quantification of beta 2-adrenergic receptor numbers in lymphocyte membrane of peripheral blood were done on all patients. Those pollinic patients who were asymptomatic at the time of the study had 500.07 +/- 237.27 receptors/cell; no significant differences were established with the control group, with 541.53 +/- 123.63 receptors/cell. However, both the control group and asymptomatic patients had receptor numbers which were significantly higher than those of symptomatic pollinic patients, with 376.81 +/- 158.65 receptors/cell (p < 0.01 and p < 0.05, respectively). The average decrease in number of receptors in symptomatic pollinic patients was 30.42% in relation to controls and 24.65% in relation to asymptomatic patients. Within the subgroup of pollinic patients, studied both in and out of season, the number of beta 2-adrenergic receptors had an average decrease of 13.22% during pollination, with 363.7 receptors/cell. Once the pollination season was over, this figure increased to 419.1 receptors/cell, establishing significant differences with p < 0.025. The number of beta 2-adrenergic receptors did not correlate with total seric IgE figures or with antigen-specific histamine release. These data indicate that the decrease of these receptors does not constitute the causal factor of atopic diseases; it seems more likely to be a consequence of the same.

Adolescent↗
PubMed1979

Structural changes in the pollinated and unpollinated avocado stigma and style.

Structural changes in the pollinated and unpollinated avocado (Persea americana Mill) stigma and style up to 42 h after first opening of the flower were investigated using light and electron microscopy. The pollen tubes grew in the stigma secretion and intercellular substance and initial contact occurred between the plasma membrane of the male and the cuticle and stigma secretion of the female. The pollen tube wall started to develop 15 min after pollination and increased in thickness up to 24 h after pollination. By 18 h after first opening of the flower, starch had disappeared and cell wall thickenings were present in both the pollinated and unpollinated stigma and style. The wall thickenings developed more slowly in the unpollinated than in the pollinated tissue. They contained lipid and were bounded by callose. Degeneration of the cytoplasm of some of the papilla and transmitting tissue cells occurred only following the passage of the pollen tubes and may be of importance in tube nutrition. There was no degeneration in the unpollinated stigma and style and the cytoplasm did not start to lose clarity until 42 h after first opening of the flower.

Cell Wall↗
PubMed1992

Model curriculum in Medical Crisis Counseling. Linda Pollin Foundation/NIMH Workshop.

This document describes a curriculum model for a year-long fellowship training program in Medical Crisis Counseling designed for health care professionals selected as Linda Pollin Fellows. The document was developed at the Second Annual Workshop, cosponsored in 1990 by the National Institute of Mental Health and the Linda Pollin Foundation. Conference participants included experts in the field of mental health--representing psychiatry, nursing, psychology, and social work--who were divided into task forces focusing on various content areas: clinical, sociocultural, managerial and administrative, liaison/educational, and research and evaluation. The document develops the content in these areas. Although designed for Pollin Fellows, the curriculum, once implemented and evaluated, may be modified for use in the basic education of a variety of health professionals. The philosophy upon which the curriculum is based incorporates the following core concepts: 1) each Fellow must have a skilled supervisor (Mentor), 2) the supervisor-trainee relationship should embody the essence of the future relationship between trainees and their patients, and 3) Medical Crisis Counseling is essentially a multidisciplinary enterprise.

Chronic Disease↗
PubMed1991

Oriental orchid (Cymbidium pumilum) attracts drones of the Japanese honeybee (Apis cerana japonica) as pollinators.

The discovery that drones of the Japanese honeybee (Apis cerana japonica) pollinate the oriental orchid (Cymbidium pumilum) is reported. Drones are attracted to the orchid flower aroma mainly during their mating flights in April through May. Some drones cluster on the flower racemes and others insert their heads deep into the flowers. Drones with pollinia on their scutellum visit other orchids, which facilitates pollination. Individual workers and swarming colonies are also strongly attracted by the flower aroma, but the allopatric western honeybee (Apis mellifera) is not attracted.

Animals↗
PubMed1979

Histochemical localization of enzymes in the stigma and style of Gossypium hirsutum L. during pre- and post-pollination stages.

The distributional pattern of some enzymes (esterase, beta-D-galactosidase, succinate dehydrogenase and malate dehydrogenase) is described in the dry stigma and closed style of Gossypium Hirsutum L. during pre- and post-pollination stages. All the four enzymes indicated granular reaction and increased activity in the transmitting tissue and its surrounding cells during post pollinated stage. The possible physiological role of these enzymes in pollen tube growth in vivo in stigma and style is discussed.

Esterases↗
PubMed2026

Resolving the "Yucca queretaroensis problem": Phylogenomic analysis of Yucca reveals the identity of an enigmatic species and the origin of an obligate pollination mutualism.

PREMISE: The genus Yucca is a group of ~50 species of woody monocots endemic to the North American arid regions. Their obligate pollination mutualism with yucca moths is considered a "textbook example" of coevolution and is hypothesized to have promoted rapid diversification. However, testing this hypothesis has been difficult due to uncertainty about the placement of a rogue taxon, Yucca queretaroensis, a rare endemic of the Sierra Gorda region of central Mexico. Past work placed this species in different positions within the Agavoideae, producing starkly different age estimates for Yucca (25 to 4 million years). METHODS: We generated new sequence capture data for 353 nuclear genes and for all coding regions of the plastid genome from wild-collected plants and samples included in previous studies to provide a new phylogeny and new age estimate for Yucca. RESULTS: The data presented here suggest that Y. queretaroensis is closely related to other species of Yucca. A relaxed molecular clock analysis of the plastid genome produced an estimated age for the genus of approximately 6.8 million years. CONCLUSIONS: The results resolve a mystery that has bedeviled evolutionary biologists for decades and provide a surprisingly young estimate for the age of Yucca, suggesting rapid diversification. The past difficulties in identifying the correct placement of Y. queretaroensis appear to be the product of laboratory errors, mistakes in field identification, and frequent hybridization with co-distributed taxa. The "Yucca queretaroensis problem" reaffirms the essential role for traditional botanical tools in phylogenomics.

ASTRAL↗
PubMed2026

Inducing fixation of transgenic alleles in open-pollinated populations.

The progeny of chlorsulfuron-resistant forage rape (Brassica napus L. subsp. napus, cv. Giant) and potato (Solanum tuberosum L. cv. Iwa) plants hemizygous for a single transgenic locus were screened for transgene segregation following an application of either water or chlorsulfuron. The transgenic locus contained three transgenes conferring kanamycin resistance (NOS-NPTII-NOS), &#x3b2;-glucuronidase (GUS) activity (35S-GUS-OCS), and chlorsulfuron resistance (a complete acetohydroxyacid synthase gene with a proline197 to serine substitution). In the absence of the herbicide application, plants segregated for GUS activity as expected for single locus inheritance. However, the progeny of plants sprayed with chlorsulfuron exhibited a highly distorted segregation for GUS activity, with a significant excess of transgenic progeny. Inducing such biased segregation provides a simple treatment to rapidly drive the fixation of transgenic alleles to homozygosity in open pollinated populations during seed increases of new cultivars.

Alleles↗
PubMed2026

Museum specimens reveal the genomic consequences of long-term population decline in an insect pollinator.

Global insect pollinator populations are under threat, with reported declines attributed to increasing habitat loss, pesticide use, and disease. Tracking how genetic diversity has changed over time could reveal the rate and extent of these declines, and the adaptive capacity of affected species-providing an important complement to habitat-based conservation efforts. However, few studies have been able to reconstruct suitable historical baselines to link genomic changes with population change. Here, we use whole genome data from 101 museum specimens of the declining moss carder bumblebee (Bombus muscorum) collected across Britain and Ireland between 1894 and 2019 to reveal a dramatic drop in genetic diversity over the last century. We find a substantial (&#x223c;24.6%) reduction in genome-wide heterozygosity across Britain during this period. In England and Wales, where habitat fragmentation is most pronounced, we observe a 2.86-fold increase in runs of homozygosity, commensurate with population fragmentation and isolation. Our results reveal the extent to which human-induced environmental change can lead to severe decadal-scale genomic erosion in a functionally important insect. Identified using DNA from historic museum collections, our approach has widespread applicability for insect conservation and understanding the evolutionary consequences of environmental change.

Animals↗
PubMed1992

Roles for the extracellular matrix in plant development and pollination: a special case of cell movement in plants.

Pattern formation in plants is now thought to be primarily dependent on positional information during development. We discuss the prevalent theories on how position is deciphered by cells in an organism and highlight the recent advances implicating molecules of the cell wall or extracellular matrix (ECM) in this process. We compare the functions of the ECM in plants and animals and describe the various cell and substrate adhesion molecules of the animal ECM which play a role in morphogenesis and cell movement. We propose that analogous molecules may occur in plants and provide evidence for the presence of a substrate adhesion molecule like vitronectin in plants and algae. We provide a model for how substrate adhesion molecules may be involved in a special case of cell movement in plants, pollination.

Animals↗
PubMedDate not supplied

Action of ketotifen on histamine release and intracellular cAMP levels in basophil cultures from pollinic subjects.

Basophils from peripheral blood of 22 pollinic patients were isolated and were cultured in the presence of different concentrations of ketotifen. We studied the effect of this drug on the specific histamine release (Technicon autoanalyzer) and the levels of intracellular cAMP during days 1, 6 and 12 of culture period. We observed that ketotifen induced an inhibition of specific histamine release throughout the culture days, but was greater on the 12th day. The cAMP levels likewise increased during the period of culture in the presence of ketotifen.

Adult↗
PubMed1989

Comparative study of maize cultivars 30 days after pollination (30 DAP): carbohydrates and protein.

A comparative study of the carbohydrates and protein of five maize cultivars at 30 days after pollination (DAP) showed a higher protein content for the Supersweet variety, and better protein quality for the Maya Opaque-2, Nutrimaiz, the Supersweet, and the Hybrid L90 x L41, when compared to the Maya Normal. The lipid content also proved to be higher in the four genetically-modified cultivars in relation to the Maya Normal. In contrast, the Maya Normal and the Opaque-2 were higher in starch content, lower in total and reducing soluble sugars, and exhibited only very small amounts (approximately equal to 1%) of phytoglycogen, a water-soluble polysaccharide. Nutrimaiz and the Hybrid L90 x L41 had approximately half the starch of the Maya Normal cultivar, but presented an appreciable content of phytoglycogen (23-26%), which is typical for sugary maize mutants, and a higher amount of soluble sugars, mainly sucrose, in relation to Maya Normal. The Supersweet had about half the starch content and a very high (approximately equal to 24%) amount of soluble sugars, mainly sucrose, in relation to the Maya Normal cultivar. The Supersweet also had a negligible amount of phytoglycogen. Nutrimaiz and the Hybrid L90 x L41 showed a much higher proportion of grain in the ears and, as a consequence, a higher yield of dry solids at 30 DAP, when compared with the Maya Normal cultivar.

Amino Acids↗
PubMed1989

Comparative study of maize cultivars 30 days after pollination (30 DAP): characterization of starch.

Characterization of starch was comparatively studied in five maize cultivars, i.e., Maya Normal, Nutrimaiz, two hybrids (L90 x L78, L90 x L41) and Supersweet (SRR "Duro"), 30 days after pollination (30 DAP). Amylose content was found to be higher in the Maya Normal starch (31.3%) and lower (23 to 27%) in the genetically-modified cultivars. The absolute density of the isolated starches did not show significant differences among cultivars. Solubility and swelling studies suggested the presence of more homogenous forces maintaining the granular matrix on the starch from Maya Normal in relation to the other maize cultivars. The starch from the genetically-modified cultivars had a reduced solubility in dimethylsulfoxide and lower values for the gelatinization temperature range, in comparison to the Maya Normal. Information from the Brabender amylogram indicated greater resistance of the gelatinized maize starches to granule breakdown by mechanical stirring, in the new genotypes, resembling those starches modified by cross-linking.

Amylose↗
PubMed1979

North American pollinosis due to insect-pollinated plants.

In warmer regions of North America many newly introduced plants are cultivated widely and others are becoming aggresive naturalized weeds. A large number are insect-pollinated and shed considerable quantities of airborne pollen. Levels of allergenicity based on skin test data, numbers of patients having immediate hypersensitivity and localities where airborne pollen grains have been identified are presented for each genus of entomophilous plants considered incitants of pollinosis. Among the most relevant are Acacia, Brassica, Citrus, Ligustrum, Olea and Schinus.

Air Pollution↗