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At least 19 recordsLinked to original sources
PubMed1984

Photomicrography of weakly fluorescent objects--employment of p-phenylene diamine as a blocker of fading.

By adding p-phenylene diamine (PPD) to the embedding medium, the fading of fluorescent objects labeled with FITC or mithramycin is substantially reduced. Thus, a multiple quantity of light, as compared to without additive, may be obtained from the objects and so photomicrography be improved or made possible at all. For microfluorometry as well as for subjective fluorescence microscopy the employment of PPD is not very helpful.

Fluorescent Antibody Technique↗
PubMed1989

Antisperm antibodies and sperm motility: a study using timed exposure photomicrography.

This study examined the effect of antisperm antibodies on sperm motility. Antisperm antibodies present in seminal plasmas with different sperm agglutinating titres were transferred passively to normal donor sperm, and the effects on movement characteristics (velocity of forward progression, amplitude of lateral head displacement, percentage progressive motility and percentage non-progressive motility) were analysed using timed exposure photomicrography. There was no significant association between sperm movement characteristics and the presence of titre of antisperm antibodies in seminal plasma. Furthermore, no differences were detected between those samples that possessed sperm agglutinating versus sperm immobilizing activity. These findings do not support the common belief that antisperm antibodies are a cause of poor sperm motility in semen.

Antibodies↗
PubMed1980

A fibre optic ring illumination system for use in low-powered dark field (including Rheinberg method) video, cine and photomicrography.

A fibre optic ring illuminator normally used as a reflected light source with a low-powered microscope is here used as a substage illuminator with a stereo zoom microscope, to give uniform dark field (dark ground) illumination. This development was primarily for use in colour video recordings giving good contrast pictures but can also be used successfully in cine and photomicrography, at magnifications ranging from X 1.5 to X 124. Its application is particularly suitable for the observation of biological specimens.

Animals↗
PubMed1975

Infrared color photomicrography of soil microorganisms.

The infrared color photomicrography technique for detecting unstained microbial cells in soil is revised for use with Kodak's recently introduced Ektachrome infrared film. Thes new version of the film required achromatic objectives instead of apochromats, and the use of a blue filter in addition to the previously required red filter. These filters also improve the visual focus capability of the operator. It was found that dry soil smears can be photographed as either immersion oil or aqueous mounts. Several makes of bright-field transmitted-light microscopes gave similar results. Phase microscopy, however, produced deteriorated image quality although the proper 'false' colors were produced.

Bacteria↗
PubMed1984

Five common problems in color photomicrography.

The practical mating of the modern microscope and automated camera has been successful. Many time-consuming photographic procedures have been reduced to a minimum in the process. The pathologist can obtain color photomicrographs with minimum knowledge of optical theory or photographic procedures. However, there are several problem areas which require special attention to ensure a constant flow of good photomicrographs. This article reviews and offers solutions to five common problems in photomicrography.

Color↗
PubMedDate not supplied

A simple method for embedding small specimens for photomicrography and sectioning following intracellular microiontophoresis of lucifer yellow CH.

A simple method for the rapid processing of small specimens following intracellular labelling with the fluorescent naphthalimide dye Lucifer Yellow CH is described which involves embedding in glycol methacrylate-based resin on cavity slides. The technique, which may be suitable for other intracellular and extracellular fluorescent markers, permits early fluorescence photomicrography of whole mounts and subsequent recovery of the specimens for serial sectioning and further analysis. In the present study on isolated human eccrine sweat glands, the procedure has facilitated both the identification of cells from which electrical records have been made and the determination of their dye-coupling status.

Eccrine Glands↗
PubMed1977

[Contribution to the problem of color reproduction in photomicrography with color films (author's transl)].

In order to define clearly the possibilities and conformities in reproducing stained microscopic specimens we need an exact system of formal co-ordination and conception. Therefore, an assay was made to find practical criterions allowing the choice of the most suited color films for the photomicrography of stained preparations. This was done by splitting up the transfer of information by adequate reproducible colors of the slides with the color space. Examples are given for Eosin bluish, for the mixture of Light green and Xylidin Ponceau and for the colors of Fujichrome R 100.

Histological Techniques↗
PubMed1975

In vivo photomicrography of the corneal endothelium.

A technique and apparatus for observing and photographing the corneal endothelium in vivo at a magnification of approximately times 200 is described. The method is suitable for animal experimentation and for diagnostic observation and clinical research in humans.

Aging↗
PubMed1981

In vivo photomicrography of the crystalline lens.

We describe a technique and apparatus for observing and photographing the crystalline lens in vivo and in the enucleated eye. Magnifications of x25 to x100 are easily obtainable on the film plane. The method is suitable for diagnostic observation and clinical research on cataract formation and prevention in humans, as well as for animal experimentation studies.

Animals↗
PubMed1998

Use of a digital film scanner to enhance low-power bright field photomicrography.

Low-power bright field photomicrographs often suffer from insufficient sharpness, uneven illumination, and colour hues. Using a film scanner, commercially available and designed for digitizing 35-mm transparencies, we directly scanned microscopic slides that carried dye-labelled and stained sections. The digital images covered a field of up to 24 x 36 mm and revealed excellent sharpness, absolutely even illumination and superior colour reproduction as compared to conventional photomicrographs taken with binoculars, macro lenses, or microscopes. As the method requires neither specialized instrumentation nor expert knowledge of photomicrographic techniques, it reduces costs and saves time. The high-quality digital survey micrographs can easily be used for image processing, image analysis and morphometry. Thus, this new method is valuable not only for pathology, embryology, histochemistry, and the neurosciences, but also for the exchange of low-power micrographs via the internet and for computer media that are increasingly used in medical education.

Image Enhancement↗
PubMed1985

Three-dimensional reconstruction from serial sections III. AUTOSCAN, a software package in FORTRAN for semiautomated photomicrography.

A FORTRAN program, called AUTOSCAN, is described. This program permits the collection of photomicrographic data from serial sections to be semiautomated. In essence the user defines a box around a microscopic field of interest. Then the program drives the stage incrementally in the x and y directions, taking photographs of contiguous subfields. The box is defined by the use of a joystick and the "return" key. That is, movements of a joystick cause the stage to translate in the x and y directions. When a corner of the object is reached, as defined by cross-hairs in the microscope eyepiece, the user hits the return key. Repetition of this process at each corner defines a "box" within which photographs are to be taken. AUTOSCAN then calculates the step size and the number of frames to be taken from the user-defined values for the magnification. The actual movements of the stage in the x and y directions and the photography are fully automated. Each frame of film has the x and y coordinates of the center of the subfield being photographed imprinted in one corner, along with other relevant data. The x and y coordinates permit the resultant information to be assembled correctly into a two-dimensional montage.

Computers↗
PubMed1999

Virtual microscopy: high resolution digital photomicrography as a tool for light microscopy simulation.

Recent advances in microcomputers and high resolution digital video cameras provide pathologists the opportunity to combine precision optics with digital imaging technology and develop new educational and research tools. We review recent advances in virtual microscopy and describe techniques for viewing digital images using a microcomputer-based workstation to simulate light microscopic examination, including scanning at low power to select features of interest and zooming to increase magnification. Hardware and software components necessary to acquire digital images of histological and cytological slides, and closely simulate their examination under a light microscope are discussed. The workstation is composed of a MicroLumina digital scanning camera (Leaf Systems, Southborough, MA), light microscope (Olympus Optical Co., Lake Success, NY), Pentium (Intel Corp., Santa Clara, CA) 166 MHz microcomputer configured with 64 megabytes of random access memory (RAM), a MGA Millenium Powerdesk graphics card (Matrox Graphics, Inc., Montreal, Canada) and Photoshop software (Adobe Systems Inc., San Jose, CA) running in a Windows 95 (Microsoft Corp., Redmond, WA) environment. Images with spatial resolutions of up to 2700 x 3400 pixels in 36-bit color, can be displayed simultaneously as distinct images in a montage, or merged into a single composite image file to highlight significant features of a histological or cytological slide. These image files are saved in Joint Photographers Experts Group (JPEG) format using compression ratios of up to 80:1 without detectable visual degradation. The advantages and technical limitations of various workstation components are addressed and applications of this technology for pathology education, proficiency testing, telepathology, and database development are discussed.

Forecasting↗