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PubMedDate not supplied

Early reproductive loss due to bovine pestivirus infection.

Bovine pestivirus infection has been mainly recognized as the cause of mucosal disease, a syndrome which occurs sporadically in weaned and adult cattle as a late sequel to foetal infection during the first 3-4 months of gestation. Infection has also been associated with the occurrence of congenital malformations, especially of the central nervous system. Following the development of improved diagnostic and research techniques, pestivirus has now been shown to be associated with significant early reproductive loss including fertilization failure, embryonic mortality and abortion. The principal determinant of the outcome of in utero infection in the bovine is the age of the conceptus when infection occurs.

Abortion, Veterinary↗
PubMed1992

Pestivirus infection of ruminants in Australia.

Pestivirus infections are commonly diagnosed in cattle but are relatively uncommon in other ruminant species in Australia. Virus isolation is a very reliable technique for detecting pestivirus in specimens, especially when group reactive monoclonal antibodies are used with immunoperoxidase staining to detect non-cytopathogenic virus. Care must be taken to prevent adventitious pestivirus contamination of serum or cells used for cell culture. A recently developed antigen capture enzyme-linked immunosorbent assay has been extensively evaluated and found to be extremely accurate. This test is also much quicker and less expensive than virus isolation. Procedures are outlined to reliably certify animals to be free of pestivirus infection for export or as donors of semen or embryos.

Animals↗
PubMed1990

Ruminant pestivirus infection in pigs.

Ruminant pestivirus infections of pigs have a worldwide distribution. The prevalence is varied and depends mainly on (i) contact with cattle, (ii) age of pigs and (iii) degree of homology of virus strains used for serology, with field strains of bovine virus diarrhoea virus (BVDV) infecting pigs. Emphasis should be laid on sources of BVDV other than cattle, e.g. contaminated vaccines and fetal calf serum. The need for differentiation of pestiviruses (hog cholera, bovine virus diarrhoea and Border disease viruses) is highlighted by the fact that clinical disease syndromes, e.g. growth retardation and wasting, are reminiscent of hog cholera. Monoclonal antibodies are available which differentiate between hog cholera virus (HCV) and ruminant pestiviruses, presumably BVDV. An up-to-date account of the antigenic relationship between pestiviruses is included in the review. Analysis of the in vitro host range of these viruses is considered to be important and may explain infections of pigs with pestiviruses other than HCV. Recent results have shown the existence of "specialists" amongst BVDV strains for bovine cells, and a few isolates also performed well in cultures of the PK15 cell line. In contrast, multipotent BVDV strains presumably have additional attachment sites for ovine and porcine cells. Identification of receptors on ovine and porcine cells could contribute to a clear distinction between BVDV and HCV infections of pigs. Immediate control measures for BVDV infections of pigs are not required. However, such infections may interfere with serologic surveys and surveillance on a herd basis and, therefore, impair eradication programmes and efforts to maintain the status in countries declared free of hog cholera.

Animals↗
PubMed1997

The effect of bovine pestivirus infection on the superovulatory response of Friesian heifers.

The pathogenesis of reproductive loss associated with bovine pestivirus infection during the preovulatory period was investigated using superovulated heifers. Twenty-five Friesian heifers were selected and randomly assigned to either a control group (n = 12) which did not become infected or to a treatment group (n = 13) which became infected following intranasal instillation of 2 ml of serum inoculum containing 5.5 log(10) TCID(50)/ml non-cytopathic virus, 9 d prior to artificial insemination (AI). Transrectal ultrasonography was used to monitor follicular development and ovulation during the superovulatory period. Animals were superovulated using a standard protocol of twice-daily injections of FSH-P and then were inseminated twice commencing 12 h after the onset of estrus. The intensity of expression of estrus was higher in the control heifers than in the pestivirus-infected heifers. Of 13 pestivirus-infected heifers, only 3 heifers displayed standing estrus compared with that in the control group, in which 10 of 12 heifers exhibited standing estrus. The mean number of ova/embryos recovered from the control group heifers was 5.75 +/-2.31, of which 4.00 +/- 0.72 were evaluated as transferable quality embryos. In comparison, heifers in the pestivirus-infected group yielded only a mean of 0.60 +/-0.34 ova/embryos, of which 0.23 +/- 0.22 were transferable quality embryos. Based on ultrasonographic examination, 24 h after the first AI 82% of the presumptive ovulatory follicles had ovulated in the control group compared with an ovulation rate of only 17% in the treated group. The results of this experiment demonstrated that bovine pestivirus infection during the preovulatory period could adversely affect ovulation, thus leading to a significant reduction in the number of palpable corpora lutea and in the number and quality of embryos recovered.

Journal Article↗
PubMed1990

Pestivirus infections in ruminants other than cattle.

Pestiviruses infect a wide range of domestic, captive and free-living ruminants. Among domestic livestock, Border disease virus is a well recognised cause of an important congenital disease of sheep in virtually all sheep-rearing countries of the world. The clinical signs, pathogenesis, diagnosis, epidemiology and control of this disease are described in detail. One natural outbreak of Border disease in domestic goats has been described and there is serological and virological evidence that pestiviruses occur widely in this species. A pestivirus has been isolated from a farmed red deer (Cervus elaphus) and there is serological evidence of a widespread low prevalence of infection among this new domestic species. Pestiviruses have been associated also with outbreaks of disease among captive ruminants in zoological collections. Among free-living ruminants, pestiviruses have been recovered from dead roe deer (Capreolus capreolus), fallow deer (Dama dama), African buffalo (Syncerus caffer), giraffe (Giraffa camelopardalis) and wildebeest (Connochaetes spp.) but in all these instances the contribution of the virus to the cause of the disease was uncertain. Serological surveys have shown that many species of free-living ruminants in North America, Europe and Africa have varying prevalence rates of antibodies to pestiviruses.

Animals↗
PubMed1999

Pestivirus infection in small ruminants: virological and histopathological findings.

Pestivirus infection was detected in several flocks of sheep and goats located in the south of Italy by means of serological, virological and histopathological investigations. From four animals, two lambs and two kids, showing enteric symptoms which died during the first week of life, four pestivirus strains were isolated and typed as BVDV-like (three isolates) and "tipic" BDV strains (one isolate). The histopathological lesions consisted of areas of hypomyelination in the brain, and a slight depletion of thymic medullary lymphocytes associated with an increase in reticular cells.

Animals↗
PubMed1995

Comparison of an antigen capture enzyme-linked assay with reverse transcription--polymerase chain reaction and cell culture immunoperoxidase tests for the diagnosis of ruminant pestivirus infections.

A study to compare the merits of three different tests for the diagnosis of ruminant pestivirus infections was carried out. Sensitivity studies using reference strains of bovine viral diarrhoea virus (BVDV) and buffy coat samples from persistently infected (PI) carriers showed the reverse transcription-polymerase chain reaction (RT-PCR) had a greater sensitivity than the other tests. The antigen capture enzyme-linked immunosorbent assay (ELISA) was least sensitive and could only be used on samples containing cells (tissue or blood). When 169 clinical samples were examined, the RT-PCR detected the most positives (42) compared to the ELISA (32) and the immunoperoxidase test (IPT) (20). The RT-PCR was more successful when specific antibody was also present in the sample. The lower sensitivity of the IPT was related to the use of a 1 passage (4-day) test and the testing of toxic or contaminated samples. The ELISA was found to be most suitable for large-scale testing for the diagnosis and control of pestivirus infections.

Animals↗
PubMed1990

Incidence, epidemiology and control of bovine pestivirus infections and disease in Australia and New Zealand.

Pestivirus infection of cattle is widespread and common in both Australia and New Zealand. The majority of adult animals, of the order of 60%, carry antibody. Associated disease is almost entirely that resulting from infection in utero. This includes death of the conceptus, at any stage from conception through pregnancy, or, in those which are born as persistently infected carriers, mucosal disease, most commonly in a chronic form. Little or no disease is recognised as a result of the post-natal infection of non-pregnant animals and these appear to be of little consequence as spreaders of infection. Transmission and enzootic maintenance depend primarily on the persistently infected carriers that are immunotolerant after early in utero infection and range clinically from normal, or nearly normal, to overtly mucosal diseased. The expulsion of an infected conceptus, and associated discharges, also provides an effective source of infection. There is generally little active control attempted. Vaccines are not available in Australia and are not widely used in New Zealand. However, interest in control is growing in those areas of the industry, especially in breeding by artificial insemination and embryo transfer, where it is perceived that the pathogenic impact of the virus may be amplified.

Animals↗
PubMed1995

Ruminant pestivirus infections in animals other than cattle and sheep.

The current knowledge on infection with ruminant pestiviruses in animals other than cattle and sheep and reviewed. Natural infection is found to occur in goats, in captive and free-living ruminants, as well as in pigs. The viral agent, susceptible species, epidemiology, clinicopathologic lesions, pathogenesis, diagnosis, control, and consequences for control programs in domestic ruminants are discussed.

Animals↗
PubMed1991

Prevalence of ruminant pestivirus infections in Namibia.

Following several clinical cases of suspected bovine virus diarrhoea (BVD) on three Namibian cattle farms, a serological survey was conducted on bovine, ovine, caprine and wild ruminant sera originating from different regions of the country. Neutralizing antibodies to BVD virus (BVDV) were detected in 58% of 1,014 cattle sera, 14% of 618 sheep sera and 4.6% of 1,118 goat sera. Sera from seven of ten wildlife species were positive with kudu, eland and giraffe having prevalence rates greater than 40%. BVDV was isolated from six clinically affected bovines and three healthy heifers persistently infected with BVDV. The survey demonstrated that pestivirus infections are widespread in Namibia in both domestic and wild ruminants.

Animals↗
PubMed2002

Pathogenesis of transplacental virus infection: pestivirus replication in the placenta and fetus following respiratory infection.

Although transplacental virus infections account for considerable morbidity and mortality in both animals and humans, very little is so far known about the pathways whereby virus reaches the conceptus, the subsequent virus-host interactions in the early phases of the infections, and the establishment of persistent non-lethal infection. Using a natural animal model we recently demonstrated that bovine pestivirus can spread from the site of infection to the ovine fetus within 72 h, despite the expression of interferon in the reproductive tract [1]. In the present study we demonstrate that pestivirus first establishes infection and spread within the allantoic and amniotic membranes and then the fetus, followed several days later by infection of the uterine glands. However, virus replication and spread within the fetus is, at least in part, controlled by fetal developmental factors. In fetuses less than 25 days of gestational age, the virus remains restricted to the bulbis cordis, the first brachial pouch and occasionally the aorta. Over the next few days the virus spreads to multiple tissues, in addition to becoming more widespread and pronounced within the initially infected tissues. A potential role for the binucleated cells of the allantochorion in the spread of the virus from the fetal to the maternal tissues was also found. These cells expressed high levels of viral antigen just prior to and during the time period in which virus antigen became detectable in the epithelial cells of the uterine glands, in endothelial cells of uterine vessels and in scattered macrophage-like cells in the uterine stroma. Most likely this relatively late virus transfer is inconsequential for the mother, since it occurs at a time when a maternal virus-specific antibody response is becoming measurable. This is in contrast to the fetus, where the infection will have established itself widely prior to the development of lymphoid tissues and a functional immune response, thus setting the scenario for development of specific tolerance to the persisting virus.

Animals↗
PubMed1997

Immunohistochemical diagnosis of pestivirus infection associated with bovine and ovine abortion and perinatal death.

OBJECTIVE: To establish a reliable, rapid, economical method for detection of pestivirus infection in bovine and ovine fetuses and to examine participation of these viruses in abortions and neonatal mortality. ANIMALS: 213 bovine and 31 ovine fetuses, as well as 36 newborn calves and 25 lambs, which had died within 3 days after birth, were tested for bovine viral diarrhea virus (BVDV) and border disease virus by use of different methods. PROCEDURE: Detection of BVDV in fetuses was performed by immunohistochemical methods, using a panel of monoclonal antibodies against pestivirus antigens on cryostat and paraffin sections and by virus isolation in cell culture; in some instances, an antigencapture ELISA was performed. Results of the various methods were compared. RESULTS: Sensitivity of BVDV detection by immunohistochemical methods and virus isolation in cell culture was equal; however, it decreased in association with autolysis. In autolytic fetuses, use of formalin-fixed, paraffin-embedded brain sections was the most favorable method. Antigen detection by ELISA was less sensitive. CONCLUSIONS: Immunohistochemical analysis of cryostat sections of brain, skin, thyroid gland, abomasum, and placenta is a rapid, sensitive method for detecting pestiviruses in fetuses. In the presence of advanced autolysis, this method used on formalin-fixed, paraffin-embedded brain sections is recommended over the other described methods.

Abortion, Veterinary↗
PubMed1991

[Use of monoclonal antibodies for the differential diagnosis of pestivirus infections in swine].

Monoclonal antibodies (mab) specific for hog cholera virus (HCV), bovine viral diarrhoea virus (BVDV) or pestivirus were applied for the differential diagnosis of pestivirus infections in pigs. Field virus isolated from 8 confirmed classical swine fever outbreaks and one suspect case was propagated in PK(15) cell cultures and identified by direct immunofluorescence (IFA) and peroxidase linked antibody (PLA) assays. Peroxidase-linked HCV, BVDV and pestivirus specific mab were applied in direct PLA for differentiation. Nine isolates were classified as members of the genus pestivirus. Eight isolates showed a positive reaction with an HCV mab. One isolates reacted with BVDV specific mab only. For further characterization an indirect PLA was performed using a collection of different HCV and BVDV specific mabs. Some of the HCV isolates also showed a weak reaction with BVDV specific mab.

Animals↗
PubMed1990

Pestivirus infections in Norway. Epidemiological studies in goats.

During one breeding season, 2335 female goats in 39 herds in different parts of Norway were examined for pestivirus infection and for reproductive performance. Before breeding, all animals were examined for neutralizing antibodies against the NADL strain of pestivirus, 83 (3.6 per cent) positive animals in 12 herds being demonstrated. The herd prevalences ranged from 1 to 63 per cent. Antibody titres varied from 1 in 4 to 1 in 2048. Of 1816 females in 30 herds for which post-breeding information was available, a total of 178 (9.8 per cent) animals in 25 of the herds demonstrated gestation failure. Three of these goats began to produce antibodies against the NADL strain during gestation. Sera from the 83 animals with NADL antibodies were titrated for neutralizing antibodies against three additional strains of pestivirus, the highest geometrical mean titre being found for antibodies against a Norwegian bovine strain.

Abortion, Veterinary↗
PubMed1997

Inhibition of pestivirus infection in cell culture by envelope proteins E(rns) and E2 of classical swine fever virus: E(rns) and E2 interact with different receptors.

Pure preparations of envelope glycoproteins E(rns) and E2 of classical swine fever virus (CSFV) synthesized in insect cells were used to study infection of porcine and bovine cells with the pestiviruses CSFV and bovine viral diarrhoea virus (BVDV). Almost 100% inhibition of infection of porcine kidney cells with CSFV was produced by 100 microg/ml E(rns). After removal of the virus no E(rns) was needed in the overlay medium (growth medium) to maintain this level of inhibition. In contrast, 100% inhibition of infection of porcine kidney cells with CSFV by 10 microg/ml E2 was only achieved when E2 was added to the overlay medium. When E2 was omitted, a maximum of 50% inhibition was achieved. This indicated that after the virus and E2 were removed from the cells, infection still occurred, by virus particles which were still bound to the cell surface. Treatment with 100 microg/ml E(rns) released these particles from the cell surface. Furthermore, E(rns) bound irreversibly to the surface of cells susceptible or unsusceptible to pestivirus infection and cell-to-cell spread of CSFV was completely inhibited by E2 but not by E(rns). These results demonstrated that E(rns) and E2 interacted with different cell surface receptors. Inhibition of BVDV infection of porcine and bovine cells by CSFV E2 suggested that CSFV E2 and BVDV E2 share an identical receptor. BVDV strain 5250 isolated from pigs was efficiently inhibited by CSFV E(rns), whereas several BVDV strains isolated from cattle were not, suggesting that the conformation of E(rns) plays a role in host tropism.

Animals↗
PubMed2001

Enzyme-linked immunosorbent assay using a virus type-specific peptide based on a subdomain of envelope protein E(rns) for serologic diagnosis of pestivirus infections in swine.

Peptides deduced from the C-terminal end (residues 191 to 227) of pestivirus envelope protein E(rns) were used to develop enzyme-linked immunosorbent assays (ELISAs) to measure specifically antibodies against different types of pestiviruses. The choice of the peptide was based on the modular structure of the E(rns) protein, and the peptide was selected for its probable independent folding and good exposure, which would make it a good candidate for an antigenic peptide to be used in a diagnostic test. A solid-phase peptide ELISA which was cross-reactive for several types of pestivirus antibodies and which can be used for the general detection of pestivirus antibodies was developed. To identify type-specific pestivirus antibodies, a liquid-phase peptide ELISA, with a labeled, specific classical swine fever virus (CSFV) peptide and an unlabeled bovine viral diarrhea virus peptide to block cross-reactivity, was developed. Specificity and sensitivity of the liquid-phase peptide ELISA for CSFV were 98 and 100%, respectively. Because the peptide is a fragment of the E(rns) protein, it can be used to differentiate between infected and vaccinated animals when a vaccine based on the E2 protein, which is another pestivirus envelope protein, is used.

Amino Acid Sequence↗
PubMed1990

Experimental pestivirus infections in newborn goat kids.

Two experiments were carried out in which 37 healthy newborn goat kids were inoculated with a non-cytopathic ovine (BDV) or a cytopathic bovine pestivirus (BVDV) by intramuscular or intracerebral injection. No kids showed signs of disease or gross lesions which could be attributed to these viruses, but inoculated kids had lower mean growth rates than the controls. Significant histological changes in the CNS of 14 kids were restricted largely to the white matter and consisted mainly of hypercellular foci comprising microglial/histiocytic cells and mild perivascular infiltration by mononuclear cells. Varying degrees of infiltration of the myocardium by lymphocytes and plasma cells were observed. All kids remained negative for neutralizing antibodies against pestivirus until 2 to 3 weeks after infection. Titres increased during the following weeks. Pestiviruses were recovered from kids necropsied 10 days after inoculation, but not from any kids killed 20 days after inoculation or later. Non-cytopathic virus was isolated from various tissues of four kids that had received BDV and three kids that had been given BVDV. Cytopathic viruses were not recovered from any kids. Mean white blood cell counts in all kids were within the normal range at 4 and 8 weeks after inoculation. The lymphocyte response to stimulation by phytohaemagglutinin was significantly increased on both sampling occasions in the BDV-inoculated kids, while in the BVDV-inoculated animals, a similar increase was seen only at 8 weeks.

Animals↗