IV. Occurrence and distribution of pesticide residues. Pesticide residues in the atmosphere.
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Residues of agricultural pesticides have the potential to enter the organism of farm animals and, subsequently, they can be transferred to milk. Infant formulas are manufactured from dried hydrolyzed cow's milk. Therefore, attention should be paid to possible contamination of the raw material for the manufacture of infant formulas with pesticide residues, especially those of persistent triazine herbicides. Dried milk based formulas are often the only food of young children in their first months of life. Thus, the control of infant milk formulas for pesticide residues is an important issue.
A gel permeation chromatographic (GPC) method for the determination of pesticide residues in fats and oils was subjected to two co-operative trials. Results for organochlorine pesticides (beta-HCH, gamma-HCH, dieldrin, p,p'-DDE and heptachlor) were compared with those obtained using an alumina column clean-up method. No significant differences in the mean values were found between the two techniques using the t-test (5% significance level). Horrat values demonstrated acceptable inter- and intralaboratory precisions for both clean-up techniques. Two organophosphorus pesticides (diazinon and propetamphos) and the synthetic pyrethroid deltamethrin were also determined using the GPC method. However, these compounds were not recoverable using the alumina column method, so no comparisons between the two methods were possible.
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The Codex Committee on Pesticide Residues (CCPR), in its development of international standards, has been considering during the last few years the implications of residues of acutely toxic pesticides in food commodities. CCPR has asked its scientific advisory body, the Joint FAO/WHO Meeting on Pesticide Residues (JMPR), for advice on the safety of the standards that are being developed. This work began in 1993. The 1994 JMPR first decided to use the 'acute reference dose' as a toxicological benchmark for a 'short-term ADI'. A number of acute reference doses have been allocated at subsequent meetings. The 1998 JMPR decided to consider the allocation of an acute reference dose whenever a full evaluation of a pesticide is undertaken. General guidance for the allocation of an acute reference dose was provided by the 1998 JMPR, which is discussed in this paper.
This work describes the development of a method for analyzing pesticide residues in olive oil by GC-MS and HPLC-MS. Pesticides were separated from the oily matrix by size-exclusion chromatography. After extraction, 20 pesticides were separated and analyzed by GC-MS and 11 others HPLC-MS in electrospray mode. The development of this method enabled us to identify and quantify the pesticides of interest.
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Pesticide residues in water and their toxicity to the freshwater shrimp (Paratya compressa improvisa) were studied in a river mouth in Takahamairi Bay, Lake Kasumigaura, from April to July in 1987. Concentrations of fenthion, diazinon and fenobucarb in water of the littoral zone were lower than that in the pelagic zone. The maximum concentration of fenthion, diazinon, iprobenfos and simetryn in water were 1.9, 0.8, 6.5, and 1.1 micrograms 1(-1), respectively. The 4-day mortality of the freshwater shrimp increased in lake water at Takahamairi Bay, reaching 50% at maximum in May. The mortality was probably due to residual insectside fenthion.
An unknown peak was detected in a GC chromatogram of many kiwi fruit extracts during analysis for pesticide residues. It was identified by GC/MS as diphenyl 2-ethylhexyl phosphate (DPEHP), used as a plasticizer and flame retardant. The concentration of DPEHP was investigated in 15 samples of kiwi fruit, and it was detected at between 0.02 and 0.14 microgram/g in 10 of the samples. It might be due to migration of DPEHP into the fruit from the printed portion of the polyethylene terephthalate (PET) package.
Four unknown peaks were detected in GC chromatograms during analysis of organophosphorus pesticide residues in foods. They were identified by using GC/MS as triethyl phosphate (TEP), tributyl phosphate (TBP), diphenyl 2-ethylhexyl phosphate (DPEHP) and N-ethyltoluenesulfoneamide (NETSA), which are used as plasticizers or flame retardants in food packaging. These chemical substances were detected in the range of tr. (below 0.01 microg/g) to 11 micro/g from 29 samples, and they were also detected in the packaging. It was supposed that they were transferred to the foods from the packaging. Furthermore, they were detected in some cereals and cereal products which contain fat and had been preserved for a long time.