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Results for “PYROGENS/chemistry”

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11 recordsLinked to original sources
PubMed1960

Studies on the pathogenesis of fever. VII. Preliminary chemical characterization of leucocytic pyrogen.

Study of the chemical properties of the pyrogenic component of rabbit polymorphonuclear leucocytes reveals it to contain an essential, non-dialyzable protein which: (a) is precipitated by perchloric acid, (b) is removed by extraction with phenol, (c) is soluble in 50 per cent methanol and 33 per cent saturated ammonium sulfate, and (d) is destroyed by the proteolytic action of both trypsin and pepsin. By combined chemical and chromatographic techniques the leucocytic pyrogen has been purified approximately 50-fold. The partially purified material contains less than 1 per cent carbohydrate, is resistant to periodate oxidation, is unaffected by extraction with butanol and contains at least two immunologically active components when tested by the Ouchterlony gel-diffusion technique. Its chemical properties distinguish it from other known pyrogenic substances which have been implicated in the pathogenesis of fever.

Animals↗
PubMed1959

The international pyrogen reference preparation.

In accordance with authorization given by the WHO Expert Committee on Biological Standardization in 1950, the National Institute for Medical Research, London, obtained samples of two bacterial pyrogens and arranged for an international study of their pyrogenic properties in rabbits. The results of this study showed that the dose response curves were flat and there was a large variation in response within and between colonies of rabbits.It was agreed that some reference preparation was needed. There was no evidence to indicate the most suitable type of preparation, and there was insufficient as regards either of the two which had been studied. It was therefore decided to make available a quantity of a preparation of the O somatic antigen of Shigella dysenteriae for the International Reference Preparation. Such a substance has been obtained for this purpose and the distribution and characteristics are described.

Animals↗