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At least 19 recordsLinked to original sources
PubMed2004

Teaching physicians geriatric principles: a randomized control trial on academic detailing plus printed materials versus printed materials only.

BACKGROUND: We compared the effectiveness of academic detailing with printed materials, versus printed materials only, on promoting geriatric knowledge among physicians. METHODS: 31 physicians were randomly assigned to receive academic detailing plus printed materials (group 1 intervention, n = 16), or printed materials alone (group 2 control, n = 15), on 5 geriatric topics identified from a needs assessment survey. Two participants withdrew from group 2, leaving 13 in the control group. Both groups received printed educational materials between baseline and time 1, and at time 2. Only group 1 received an additional 15-minute, one-on-one education session with a geriatrician at time 2. The primary endpoint was knowledge retention, measured by the numeric score of a 5-item questionnaire (range 0 to 5), comprised of items from the Geriatrics Knowledge Test. Knowledge retention was measured at baseline, 1 week (time 1), and on average 29 weeks later (time 2). RESULTS: Most participants were postgraduate trainees. The mean knowledge score in all participants decreased from 3.6 +/- 1.2 at baseline to 3.1 +/- 1.2 at time 1 (p =.006). 19 participants (9 in group 1 and 10 in group 2) completed the knowledge questionnaire at time 2. At baseline, group 1 scored lower than group 2 (3.4 +/- 1.3 versus 3.8 +/- 1.1, p =.39); whereas at time 2, group 1 scored significantly higher than group 2 (4.7 +/- 0.7 versus 3.9 +/- 0.7, p =.034). Academic detailing plus printed materials produced higher mean score change from baseline (1.1 +/- 1.3) than printed materials alone (0.0 +/- 1.1, p =.053). CONCLUSIONS: Academic detailing plus printed materials improved knowledge retention among physicians, whereas printed materials only did not.

Education, Medical, Continuing↗
PubMed2004

Tissue-print and print-phoresis as platform technologies for the molecular analysis of human surgical specimens: mapping tumor invasion of the prostate capsule.

Molecular profiling of human biopsies and surgical specimens is frequently complicated by their inherent biological heterogeneity and by the need to conserve tissue for clinical diagnosis. We have developed a set of novel 'tissue print' and 'print-phoresis' technologies to facilitate tissue and tumor-marker profiling under these circumstances. Tissue printing transfers cells and extracellular matrix components from a tissue surface onto nitrocellulose membranes, generating a two-dimensional anatomical image on which molecular markers can be visualized by specific protein and RNA- and DNA-detection techniques. Print-phoresis is a complementary new electrophoresis method in which thin strips from the print are subjected to polyacrylamide gel electrophoresis, providing a straightforward interface between the tissue-print image and gel-based proteomic techniques. Here we have utilized these technologies to identify and characterize markers of tumor invasion of the prostate capsule, an event generally not apparent to the naked eye that may result in tumor at the surgical margins ('positive margins'). We have also shown that tissue-print technologies can provide a general platform for the generation of marker maps that can be superimposed directly onto histopathological and radiological images, permitting molecular identification and classification of individual malignant lesions.

Biomarkers↗
PubMed2003

Writer identification using hand-printed and non-hand-printed questioned documents.

Several federal district court judges have recently referred to the purported lack of information on the proficiency of forensic document examiners (FDEs) in identifying writers of hand-printed documents. In order to provide the necessary information, we have re-analyzed data on writer identification that were collected in 1996 from 90 forensic document examiners and 34 laypersons. These data were used previously to assess the proficiency of FDEs using handwritten documents in several different types of writing. In the new analysis we separated data on hand-printed (HP) documents from data on non-hand-printed (NHP) documents and compiled error rates and statistics in each category. The main findings are: (1) whether or not the documents were hand-printed, the performance of FDEs was much better than that of laypersons; (2) statistical tests found no difference between the data provided by the FDEs in the HP and NHP categories; (3) statistical tests found no difference between the data provided by laypersons in the HP and NHP categories; and (4) statistical tests found differences between the data provided by the FDEs and the laypersons in both the HP and NHP categories. Similar results were obtained when hand-printed documents were compared to cursive documents and when cursive documents were compared to non-cursive documents. All the evidence indicates that in our proficiency test the performance of FDEs in writer identification was much better than the performance of laypersons in each one of the following document categories: (1) hand-printed; (2) non-hand-printed; (3) cursive; and (4) non-cursive.

Data Interpretation, Statistical↗
PubMed2007

Increasing print awareness in preschoolers with language impairment using non-evocative print referencing.

PURPOSE: This study examined the extent to which using non-evocative, explicit referencing of print concepts during shared storybook reading in the context of language therapy facilitated print concept knowledge in children with language impairment. METHOD: Five children, ages 4 to 5 years, were provided scripted input on 20 print concepts during shared storybook reading that was incorporated into individualized 30-min language intervention sessions that were conducted in the children's classroom twice weekly. The children were not required to make any response to the input on print concepts, and the input was secondary to instruction in the language targets during the 10-min shared storybook reading activity. RESULTS: Using a single-subject, multiple probe design across subjects, results indicated that children's knowledge of print concepts improved markedly when the procedure was incorporated into shared storybook reading and that they continued to learn and maintain knowledge of print concepts with repeated input. CLINICAL IMPLICATIONS: These findings suggest that children with language impairment may benefit from simple non-evocative, explicit referencing strategies that can be easily incorporated into the context of storybook reading during language therapy, thus providing speech-language pathologists with an additional tool for facilitating children's literacy skills.

Awareness↗
PubMed2001

Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules.

A novel totally screen-printed flow-through cell for immunoanalysis is presented. It contained screen-printed carbonaceous electrodes, which allowed the determination of peroxidase activity through the electrochemical reduction of p-benzoquinone. As different electrode materials differ strongly in their electrochemical properties, electrodes resulting from various screen-printable carbonaceous pastes were characterized using the hydroquinone/ p-benzoquinone redox couple. For most of the electrodes, cyclic voltammogram peak separations of between 550 and 670 mV were observed indicating only quasi-reversible electrochemical behavior. This was confirmed by variation of the peak separation with scan rate. Heterogeneous electron transfer rates of ca. 0.5 - 1 x 10(-3) cm s(-1) and electrochemical activation energies of ca. 20 kJ mol(-1) were found. These flow-through cells were not only applied to electrochemical peroxidase activity determinations but also, in combination with a separate detector, as affinity reactors. After biotinylation of screen-printed layers, streptavidin and then biotinylated peroxidase could be bound. However, as signals were only 10-20% of those obtained with a column filled with biotinylated glass beads, only the screen-printed electrochemical detector was applied to the detection of antibodies against the African Swine Fever Virus.

Animals↗
PubMed2005

Bacterial printing press that regenerates its ink: contact-printing bacteria using hydrogel stamps.

This paper describes the use of micropatterned agarose stamps prepared by molding against PDMS masters to print patterns of bacteria on agar plates. Topographically patterned agarose stamps were inked with suspensions of bacteria; these stamps generated patterns of bacteria with features as small as 200 microm over areas as large as 50 cm2. Stamps with many small features (>200 microm) were used to study patterns of bacteria growing on media containing gradients of small molecules; stamps with larger features (>750 microm) were used to print different strains of bacteria simultaneously. The stamp transfers only a small percentage of cells that are on its surface to the agar at a time; it is thus possible to replica-pattern hundreds of times with a single inking. The use of soft stamps provides other useful functions. Stamps are easily customized to provide a range of patterns. When culture media is included in the agarose stamp, cells divide and thrive on the surface. The resulting "living stamp" regenerates its "ink" and can be used to pattern surfaces repetitively for a month. This method is rapid, reproducible, convenient, and can be used to control the pattern, spacing, and orientation between colonies of different bacteria.

Aliivibrio fischeri↗
PubMed2005

Submerged microcontact printing (SmuCP): an unconventional printing technique of thiols using high aspect ratio, elastomeric stamps.

A technique for microcontact printing of thiols in liquid media is presented. Elastomeric poly(dimethyl siloxane) stamps are used to pattern gold surfaces with thiol-based self-assembled monolayers. The liquid (water in this case) has been used as an incompressible support and, advantageously, also acts as a medium in which alkylthiol ink molecules are poorly miscible. Consequently, we have been able to produce patterned thiol monolayers using stamps with aspect ratios unsuitable for conventional microcontact printing (i.e., 15:1) and present evidence to suggest that it is possible to use stamps with aspect ratios of up to 100:1.

Journal Article↗
PubMed2002

PRINTS and PRINTS-S shed light on protein ancestry.

The PRINTS database houses a collection of protein fingerprints. These may be used to make family and tentative functional assignments for uncharacterised sequences. The September 2001 release (version 32.0) includes 1600 fingerprints, encoding approximately 10 000 motifs, covering a range of globular and membrane proteins, modular polypeptides and so on. In addition to its continued steady growth, we report here its use as a source of annotation in the InterPro resource, and the use of its relational cousin, PRINTS-S, to model relationships between families, including those beyond the reach of conventional sequence analysis approaches. The database is accessible for BLAST, fingerprint and text searches at http://www.bioinf.man.ac.uk/dbbrowser/PRINTS/.

Amino Acid Motifs↗
PubMed2006

Spreading on and penetration into thin, permeable print media: application to ink-jet printing.

This paper examines spreading and penetration of surfactant-laden drops on thin-permeable media with reference to ink-jet printing. A detailed review of the interaction of both pure liquids and surfactant containing solutions with porous substrates is given for individual spreading and penetration and for the combined processes. A new model based on energy arguments is derived and compared to current hydrodynamic equations used to describe simultaneous spreading and penetration. Three studies of how surfactant solutions interact with thin commercial ink-jet photographic quality papers are presented. Here, two relevant systems are examined: Tergitol 15-S-5 and 1,2-octanediol. The first study examines the spreading and penetration profiles for surfactant solutions over a range of concentrations spanning their critical micelle concentration. As expected, these profiles depend on the concentration of surfactant and the chemistry of the medium with which it interacts. In many cases, partial vertical penetration of the region directly beneath the drop dominates at low interaction times and will be significant in ink-jet applications. The second study consists of a parametric investigation of the energy-based model derived herein. It shows that the model can capture all of the behaviors observed in the first study. In the final study, the ability of the energy-based model to fully predict the spreading behavior of Tergitol 15-S-5 solutions is tested. It is found that the model produces good quantitative agreement at the highest concentrations and, as such, will be useful in screening spreading dynamics concentrated systems like ink-jet inks. Agreement at low to intermediate concentrations is often limited by finite induction periods prior to significant spreading and penetration. Possible corrections that could improve the agreement for weakly concentrated solutions are discussed, and directions for future studies of simultaneous spreading and penetration are proposed.

Journal Article↗
PubMed2000

PRINTS-S: the database formerly known as PRINTS.

The PRINTS database houses a collection of protein family fingerprints. These are groups of motifs that together are diagnostically more potent than single motifs by virtue of the biological context afforded by matching motif neighbours. Around 1200 fingerprints have now been created and stored in the database. The September 1999 release (version 24.0) encodes approximately 7200 motifs, covering a range of globular and membrane proteins, modular polypeptides and so on. In addition to its continued steady growth, we report here several major changes to the resource, including the design of an automated strategy for database maintenance, and implementation of an object-relational schema for more efficient data management. The database is accessible for BLAST, fingerprint and text searches at http://www.bioinf.man.ac. uk/dbbrowser/PRINTS/

Database Management Systems↗