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At least 19 recordsLinked to original sources
PubMed1963

TIME-LAPSE PHOTOMICROGRAPHY OF THE FORMATION OF A FREE SPHERICAL GRANULE IN AN ESCHERICHIA COLI CELL END.

Hoffman, Heiner (New York University, New York), and Michael E. Frank. Time-lapse photomicrography of the formation of a free spherical granule in an Escherichia coli cell end. J. Bacteriol. 86:1075-1078. 1963.-Only a single case of the formation of a free spherical granule at an Escherichia coli cell end was found among several thousand cells recorded by time-lapse photomicrography. The spherical end body apparently arose immediately upon or soon after binary fission, at the newly formed cell end. Several minutes elapsed between the appearance of the end body and its full separation, apparently by constriction, from the mother cell. The end body showed no cytological changes during a 51-min period of observation after its separation from the mother cell. A hypothesis concerning the nature of end bodies and the mechanisms underlying their production is presented.

Cytoplasmic Granules↗
PubMed1965

TIME-LAPSE PHOTOMICROGRAPHY OF CELL GROWTH AND DIVISION IN ESCHERICHIA COLI.

Hoffman, Heiner (New York University, New York, N.Y.), and Michael E. Frank. Time-lapse photomicrography of cell growth and division in Escherichia coli. J. Bacteriol. 89:212-216. 1965.-Photomicrographs at 15-sec intervals of cells growing at 37 C disclosed that in a cell with a generation time of 21.0 min the processes of furrowing, cross-wall formation, and cell separation are completed within 2.5 min after the division furrow first becomes clearly visible. Among a large number of cultivations examined, only a few cells late in one microculture at 43.5 C failed to separate once the cross wall was completed. Measurements of cell lengths during a 5-min period, extending from just before to just after division, showed that elongation of the cell is a discontinuous process, although the growth rate over the 5-min period is exponential. At the time of cell division, it appears that the synthesis of cell-wall material is diverted entirely into formation of the cross wall.

Cell Cycle↗
PubMed1984

Photomicrography of weakly fluorescent objects--employment of p-phenylene diamine as a blocker of fading.

By adding p-phenylene diamine (PPD) to the embedding medium, the fading of fluorescent objects labeled with FITC or mithramycin is substantially reduced. Thus, a multiple quantity of light, as compared to without additive, may be obtained from the objects and so photomicrography be improved or made possible at all. For microfluorometry as well as for subjective fluorescence microscopy the employment of PPD is not very helpful.

Fluorescent Antibody Technique↗
PubMed1989

Antisperm antibodies and sperm motility: a study using timed exposure photomicrography.

This study examined the effect of antisperm antibodies on sperm motility. Antisperm antibodies present in seminal plasmas with different sperm agglutinating titres were transferred passively to normal donor sperm, and the effects on movement characteristics (velocity of forward progression, amplitude of lateral head displacement, percentage progressive motility and percentage non-progressive motility) were analysed using timed exposure photomicrography. There was no significant association between sperm movement characteristics and the presence of titre of antisperm antibodies in seminal plasma. Furthermore, no differences were detected between those samples that possessed sperm agglutinating versus sperm immobilizing activity. These findings do not support the common belief that antisperm antibodies are a cause of poor sperm motility in semen.

Antibodies↗
PubMed1980

A fibre optic ring illumination system for use in low-powered dark field (including Rheinberg method) video, cine and photomicrography.

A fibre optic ring illuminator normally used as a reflected light source with a low-powered microscope is here used as a substage illuminator with a stereo zoom microscope, to give uniform dark field (dark ground) illumination. This development was primarily for use in colour video recordings giving good contrast pictures but can also be used successfully in cine and photomicrography, at magnifications ranging from X 1.5 to X 124. Its application is particularly suitable for the observation of biological specimens.

Animals↗
PubMed1975

Infrared color photomicrography of soil microorganisms.

The infrared color photomicrography technique for detecting unstained microbial cells in soil is revised for use with Kodak's recently introduced Ektachrome infrared film. Thes new version of the film required achromatic objectives instead of apochromats, and the use of a blue filter in addition to the previously required red filter. These filters also improve the visual focus capability of the operator. It was found that dry soil smears can be photographed as either immersion oil or aqueous mounts. Several makes of bright-field transmitted-light microscopes gave similar results. Phase microscopy, however, produced deteriorated image quality although the proper 'false' colors were produced.

Bacteria↗
PubMed1984

Five common problems in color photomicrography.

The practical mating of the modern microscope and automated camera has been successful. Many time-consuming photographic procedures have been reduced to a minimum in the process. The pathologist can obtain color photomicrographs with minimum knowledge of optical theory or photographic procedures. However, there are several problem areas which require special attention to ensure a constant flow of good photomicrographs. This article reviews and offers solutions to five common problems in photomicrography.

Color↗
PubMedDate not supplied

A simple method for embedding small specimens for photomicrography and sectioning following intracellular microiontophoresis of lucifer yellow CH.

A simple method for the rapid processing of small specimens following intracellular labelling with the fluorescent naphthalimide dye Lucifer Yellow CH is described which involves embedding in glycol methacrylate-based resin on cavity slides. The technique, which may be suitable for other intracellular and extracellular fluorescent markers, permits early fluorescence photomicrography of whole mounts and subsequent recovery of the specimens for serial sectioning and further analysis. In the present study on isolated human eccrine sweat glands, the procedure has facilitated both the identification of cells from which electrical records have been made and the determination of their dye-coupling status.

Eccrine Glands↗
PubMed1977

[Contribution to the problem of color reproduction in photomicrography with color films (author's transl)].

In order to define clearly the possibilities and conformities in reproducing stained microscopic specimens we need an exact system of formal co-ordination and conception. Therefore, an assay was made to find practical criterions allowing the choice of the most suited color films for the photomicrography of stained preparations. This was done by splitting up the transfer of information by adequate reproducible colors of the slides with the color space. Examples are given for Eosin bluish, for the mixture of Light green and Xylidin Ponceau and for the colors of Fujichrome R 100.

Histological Techniques↗