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Eliminative split of pectic substances by phytopathogenic soft-rot bacteria.

Culture filtrates of Erwinia carotovora and Bacillus polymyxa split pectic substances to yield a product that absorbs at 230 mmicro, and which reacts with thiobarbituric acid to form a substance that absorbs at 547 mmicro. This product is believed to be a C-4,5-unsaturated oli-gouronide. The preferred substrate is polygalacturonic acid rather than pectin; the enzyme, provisionally named polygalacturonic-trans-eliminase, is activated by calcium.

Bacillus↗