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Development, survival, and oviposition of the rabbit tick, Haemaphysalis leporispalustris (Packard) (Acari: Ixodidae), at constant temperatures.

The relationship between development, survival, and oviposition rates, and five constant temperatures is described for the developing and reproductive life stages of the rabbit tick, Haemaphysalis leporispalustris (Packard). Development was most rapid at 29.7 C for egg and larvae, and 35.6 C for nymphs. Survival was greatest between 14.7 and 29.7 C, but was reduced at 35.6 C for eggs and larvae. Oviposition was optimal between 19.9 and 29.7 C with a maximum mean daily oviposition of 253 eggs/female/day on the third day of oviposition at 29.7 C. Maximum mean total eggs per female produced was 1,157 with a range of 277 to 3,327. The total number of eggs produced per female correlated with the weight of the engorged female. Both linear and sigmoid curve equations were used to approximate the relationship between the temperature and development rates of eggs, larvae, and nymphs. The nymphs had the highest heat requirements of the 3 development stages. Comparisons with field host-parasite data indicate that the rabbit tick has a 2-yr life cycle in southwestern Nova Scotia.

Animals

Oviposition site preference in Drosophila.

Comparative studies of oviposition site preference (OSP) in Drosophila suggest that choice of oviposition site is an important adaptive behavior which influences individual fitness and the potential of populations for speciation. OSP has been investigated under conditions which provided females with a choice of standard medium or medium containing ethanol for oviposition. OSP is an extremely labile behavior in the laboratory, but a technique has been developed which minimizes variation between replicates and allows the detection of OPS differences between semispecies of a single species. An analysis of the OSP of 14 Drosophila species shows that this behavior is not correlated with phylogenetic relationships. OSP with respect to ethanol may be correlated with the presence of ethanol in the environment and the activity of alcohol dehydrogenase in the species tested.

Animals

Light trap collections of ovipositing Simulium squamosum in Ghana.

At a site near Boti waterfalls in southern Ghana a total of 14 644 female and two male Simulium squamosum were caught in four nights in Monks Wood light traps. The highest catch, of 6520 females, was obtained in a single night with an ultraviolet light tube that had a one-second flash rate. About 12% of the females caught were gravid and dissections of non-gravids showed that they had oviposited very recently. The traps were clearly catching females before or soon after oviposition. The same traps caught none or very few blackflies when placed in two other localities near Akosombo. Trap location appeared very important in sampling ovipositing females of S. squamosum.

Animals

Emptying of the uterovaginal sperm storage glands in the absence of ovulation and oviposition in the domestic hen.

Domestic hens were treated with pregnant mare serum to study the temporal pattern of sperm gland emptying in the absence of ovulation and oviposition. After a cessation of ovulation and oviposition, the hens were artificially inseminated with .034 ml of pooled semen. Subsequent histological examination of the uterovaginal sperm glands on days 1, 6, and 12 post-insemination indicated that the temporal rate of sperm-gland emptying was not significantly different from that of control birds which were laying normally. This observation suggests that ovulation and oviposition, along with their assoicated humoral factors, have a minimal effect on the emptying of the uterovaginal sperm storage glands.

Animals

Penetration into caterpillar cells of virus-like particles injected during oviposition by parasitoid ichneumonid wasps.

Particles originating from the ovarial calyx epithelium of two different species of ichneumonid wasp are injected into host caterpillars during oviposition. At 1 3/4 h post oviposition, many calyx fluid particles are either associated with or have oenetrated through the basement membranes surrounding various tissues. Shortly thereafter, apparently intact particle nucleocapsids are observed in both the cytoplasm and nucleus of host cells. An unusual tubular protrusion of the viral envelope appears to be involved in either or both of penetration of basement membranes and entry of nucleocapsids into host cells.

Animals

Plasma concentrations of progesterone and corticosterone during the ovulation cycle of the hen (Gallus domesticus).

The observation that injections of either ACTH or corticosterone induced ovulation in the hen has prompted speculation which implicates the adrenal gland in the mechanisms controlling ovulation. In this study, the role of the major adrenal steroid in birds was further examined by measuring the plasma concentration of corticosterone during the 24 hr period preceding mid-sequence ovipositions and the 28 hr period preceding terminal ovipositions. Progesterone, which is an accurate index of preovulatory ovarian activity, was also measured in the same samples. An increase in plasma progesterone preceded mid-sequence ovipositions which are accompanied by an ovulation. This preovulatory surge in plasma progesterone began approximately 7 hr before ovulation, reached a maximum of 6.9 ng/ml 3 to 2 hr before ovulation, and returned to baseline concentrations at the time of ovulation. A major preovulatory peak of progesterone was not observed during the interval between sequences, although a small but statistically significant rise in progesterone was observed between midnight and 0600 hr on the day of the last oviposition of the sequence. The concentration of corticosterone increased approximately two-fold during the dark portion of the photoperiod regardless of the position of the oviposition in the sequence. Baseline concentrations during the illuminated portion of the photoperiod ranged between 1 and 4 ng of corticosterone per ml of plasma whereas during the period of darkness, they ranged between 2 and 6 ng of corticosterone per ml of plasma. It was concluded, therefore, that the circulating concentration of corticosterone was regulated by a circadian rhythm which operates independently of follicular maturation.

Animals

Alteration of structure and penetrability of the vitelline envelope after passage of eggs from coelom to oviduct in Xenopus laevis.

The vitelline envelope (VE) that surrounds an egg released from the ovary into the coelom of Xenopus laevis differs markedly, in structure and penetrability, from the VE surrounding an oviposited egg. In a coelomic egg, the filaments that form the VE are arranged in distinct fascicles or bundles. The exterior surface of the VE is irregular in contour and is permeated by channels. In an oviposited egg, the filaments are evenly dispersed and lack a fasciculated arrangement; the exterior surface is smooth and no channels are present. The fascicular arrangement of fibrils in the coelomic VE is maintained only at neutral pH, and is not visibly altered by the cortical reaction. VEs from coelomic eggs retain their fasciculated morphology after isolation from the egg. In an in vitro test system, sperm penetrated VEs isolated from oviposited eggs, but failed to penetrate VEs isolated from coelomic eggs. The structural transformation of the VE from the coelomic type to the oviposited type occurs in the first 1-cm segment of the oviduct, prior to addition of jelly to the egg. Neither intact jelly, solubilized jelly, nor jelly extracts were capable of altering the structural organization of coelomic VEs, suggesting that the structural transformation of the VE is effected by some oviducal factor other than jelly.

Animals