THE NEURILEMMA CELL IN PERIPHERAL NERVE DEGENERATION AND REGENERATION.
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Central neurilemma of the mandible.
A rare case of symptomless central neurilemmoma of the mandible is reported. Post-operative recovery was uneventful and, in particular, post-surgical paraesthesia was followed by a return to normal sensation.
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Ultrastructure of the red pulp in spleen innervation in horse and pig.
The innervation of the red pulp in the spleen of horse and pig was investigated by electron microscopy. In addition, the neurilemma was studied by immunohistochemistry specific for S-100 protein. In the pig, a large population of smooth-muscle cells extending from the smooth-muscle trabeculae was present in the red pulp. The cytoplasmic processes of reticular cells enwrapped the smooth-muscle cells, and nerve fibres were distributed between the smooth-muscle cells and the reticular cells. The nerve terminals clustered toward the facing of the smooth-muscle cells. Nerve fibres and terminals were not disclosed within the sheathed artery. Immunohistochemically the neurilemma showed a reaction positive for S-100 protein. In the horse, no smooth-muscle cells were noted in the red pulp. The nerve fibres terminated around the cytoplasmic processes of the reticular cells. Nerve fibres and terminals were disclosed within the sheathed artery, and the terminals contained both large and small dense-cored vesicles. Immunohistochemically the neurilemma showed a reaction negative for S-100 protein. These findings support the presence of the axon-bearing reticular cells described earlier in the horse spleen.
Animals↗
Metamorphic changes in the brain of Chironomus dolichotomus (Diptera:Chironomidae).
The larval brain lacks external differentiation of the three neuromeres. The neurilemma is well developed but the perineurium has few sheath cells. The central complex is represented by well developed and elongated central and ellipsoid bodies. The corpora pedunculata are feebly developed and only two optic lobe anlagen are differentiated from the lateral projections of the larval protocerebrum. The deutocerebrum is represented by a feebly developed medullary mass whereas the tritocerebrum is not well differentiated. In adult, the ellipsoid body becomes much conspicuous and the central body fits in the anterior concavity of the former. The corpora pedunculata has well developed calyx, stalk and beta lobes. The medulla externa becomes enormously large and the lobula and lobula plate get cut off from the protocerebrum. A single optic commissure joins the medulla externa of opposite sides. The deutocerebrum lacks nodulation and deutocerebral commissure joins the two opposite lobes. The tritocerebrum remains feebly developed. In pupal period the neurilemma becomes wavy and degenerates at certain places, however, its complete degeneration is never noted. Haemocytes are not found around the neurilemma. The sheath cells become vacuolated. Ventral tubercles appear on the posterior region of the ellipsoid body.
Animals↗
Cytochemical detection of nucleoside diphosphatase activity in the central nervous system of the rat.
The cytochemical localization of nucleoside diphosphatase and thiamine pyrophosphatase occurs within the "mature face" of the Golgi apparatus and over the neurilemma in neurons of the cerebellum, the cerebral cortex and the brain stem. The hydrolytic reaction product of the brain enzyme differs from that of the liver in that it is not found in the endoplasmic reticulum or nuclear envelope. Hydrolysis of IDP, UDP or GDP is not greater than that of ADP or CDP in brain homogenates, in contrast to that found in the liver. The NDPase activity of brain homogenates is optimal at pH 7.2, stimulated by heavy metals and inhibited by uranyl nitrate. Thick section cytochemistry suggests that the reaction product is restricted to a network of polygonally shaped compartments. NDPase activity on the neurilemma may reflect the role of this enzyme in the synthesis of glycoproteins involved in neuronal surface recognition.
Animals↗
Ultrastructural localization of Na,K-ATPase in the gerbil cochlea.
The transport enzyme Na,K-ATPase has been localized to several different cell types within the inner ear by enzyme cytochemistry, immunohistochemistry, and in situ hybridization. Although these histochemical procedures have provided a fairly consistent pattern of the enzyme's distribution, the precise location of Na,K-ATPase in the cell membrane of some polarized and non-polarized cell types remains uncertain. We addressed this problem in the gerbil cochlea using electron microscopic immunogold cytochemistry. The results confirmed prior ultrastructural localization of Na,K-ATPase along the basolateral plasma membrane of strial marginal and outer sulcus epithelial cells but differed from a previous report in failing to detect the enzyme at the surface of strial intermediate cells. The findings also concurred with and extended previous work in showing immunogold labeling along the entire cell membrane of non-polarized Type II fibrocytes in the inferior portion of the spiral ligament and of subpopulations of fibrocytes in the suprastrial and supralimbal regions. Our observations agreed further with light microscopic immunostaining in displaying uniform gold labeling for Na,K-ATPase in the neurilemma of Type I spiral ganglion neurons, even though these cells are completely ensheathed by myelin. Surprisingly, the enzyme was detectable in the neurilemma of afferent but not that of efferent nerve processes beneath hair cells.
Animals↗
[Effect of different allogenic cells injected into denervated muscles on nerve regeneration in rats].
OBJECTIVE: To study the effect of allogenic different cells injected into denervated muscles on nerve regeneration. METHODS: Thirty-six adult female SD rats, weighted 120-150 g, were divided into four groups randomly (n = 9, each group). Left sciatic nerves were cut down on germfree conditions and given primary suture of epineurium. Different cells were injected into the muscles of calf at once after operation every seven days and in all four times (group A: 1 ml Schwann cells at concentration of 1 x 10(6)/ml; group B: 1 ml mixed cells of Schwann cells and myoblast cells at concentration of 1 x 10(6)/ml; group C: 1 ml extract from the culture medium of kidney endothelial cells; and group D: 1 ml culture medium without FCS as control). After 3 months, the specimen was observed on macrobody and histology, and the densities of neurilemma cell and myoceptor were counted. RESULTS: The means of proximate neurilemma cells were 0.1877 +/- 0.0542 in group A, 0.1551 +/- 0.0321 in group B, 0.0724 +/- 0.0237 in group C, and 0.1877 +/- 0.0542 in group D. The densities of myoceptor were 6.000 +/- 0.866 in group A, 9.000 +/- 2.291 in group B, 12.780 +/- 1.394 in group C, 3.110 +/- 0.782 in group D. CONCLUSION: Schwann cells, mixed cells of Schwann cells with myoblast cells, and the extract from kidney endothelial cells can all accelerate the nerve regeneration. And the effect of extract from the kidney endothelial cell is superior to that of Schwann cell and mixed cell.
Animals↗
[Distribution and origin of corpola amylacea in the vestibulocochlear nerve].
The distribution of the corpora amylacea in the vestibulocochlear nerve is very limited. We studied the relation between the number of corpora amylacea in the cochlear nerve and the aging of cochlea and developed some hypotheses on the origin of corpora amylacea. This study involved 11 subjects, 14 samples. The results were as follows. There was no relationship between the number of corpora amylacea in the cochlear nerve and aging of the cochlea. Corpora amylacea probably have no relation to cochlear disorders. However, it was reported that corpora amylacea were produced more than 10 years after the organ's obstruction. There is a possibility that corpora amylacea increased 10 years after disorders developed in the cochlear nerve. There is a neurilemma-Schwann sheath junction (NSS junction) in the vestibulocochlear nerve. Corpora amylacea characteristically exist only in the central portion of the vestibulocochlear nerve on one side of the NSS junction. There are 3 possibilities regarding the origin of corpora amylacea which include consideration of the hypothesis that the NSS junction is the point where the vestibulocochlear nerve pierces the encephal dura mater. 1) Supposing that the origin of the corpora amylacea is the vestibulocochlear nerve itself, we would expect that the oligodendrocytes which are components of the vestibulocochlear nerve to be the origin of the corpora amylacea because they exist only in the central portion of the nerve, but not in peripheral portions of the nerve. 2) Supposing that the origin of the corpora amylacea is not the vestibulocochlear nerve itself, we would expect that the perineurium from the encephal dura mater to be the origin of corpora amylacea.(ABSTRACT TRUNCATED AT 250 WORDS)
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[Metallophilia in the Ranvier's nodes of nerve fibers in rats of different strains (histochemical study)].
Myelinated nerve fibers formed by the processes of LV spinal ganglion neurons were studied in two lines of rats selected according to high and low thresholds of nerve system excitability to electric current. Before ultrastructural study the fibres have been treated with potassium pyroantimonate. It was demonstrated that specific dense precipitate was deposited in the nodes of Ranvier of the nerve fibers of rats with low excitability thresholds; this precipitate was not found in the fibers of rats with high thresholds. It is suggested that deposition of precipitate is indicative of a high density of sodium channels in neurilemma, i.e. of high functional activity of nerve fibers.
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[Ultrastructural changes of receptor endings in prolonged action of local anesthetics].
Ultrastructural changes of the terminal plates of the bushy receptors in the frog urinary bladder have been studied after two hours' exposition in 0.05% novocaine solution and one hour's exposition in 0.05% dicaine and trimecaine solution. During these periods a steady block of the receptor impulse activity develops. The local anesthetics essentially change ultramicroscopic structure of the terminals. The reaction to the anesthetics investigated has both some features in common and certain peculiarities. At each effect three types of changes can be determined, characterized with various degree of rearrangement in neurilemma, neuroplasm and organelles. Each type of the changes is supposed to reflect a certain phase of the plate reactive response. Specificities of the reaction to novocaine are minimal changes of mitochondria, accumulation of glycogen granules, deformity and decreasing amount of vesicles. Under dicaine effect mitochondria do not change, amount of vesicles increases, their form does not change; under trimecaine effect mitochondria undergo most noticeable alterations. The changes of the terminal plates observed are interpreted as adaptive. The effect of the local anesthetics on the receptors is not limited with the blockade of the sodium canals of the afferent fibers, in parallel, biochemical processes, occurring in cytosol of the terminals also change; their morphological manifestations are the ultrastructural changes observed.
Anesthetics, Local↗
[Ultrastructure of the terminals of bushy receptors].
Patches of bushy receptors in the frog (Rana temporaria) urinary bladder cervix were revealed electron microscopically. The patches have no neurilemma, have specific topography and distribution, they vary in their form and size, their ultrastructural features are presented as specialization of cytolemma, mitochondria of a condenced type, a peculiar receptory matrix, various structure of the basal membrane in different areas. Topographic, morphological and ultrastructural data evidence in favour of a sensitive nature of the bushy receptory terminals described.
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Fine structure of the compound eyes and interfacetal mechanoreceptors of Cicindela tranquebarica Herbst (Coleoptera: Cicindelidae).
The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting only of microvilli from retinula cell seven, a more proximal, rectangular, fused rhabdom formed from six retinula cells, and a basal, eighth retinula cell with a spherical rhabdomere comprise the light sensitive portions of the ommatidium. The "subcorneal layer" consists of lamellae of endocuticular microfibrils and, in surface view, shows 11 concave polygons. Proximal extensions of the crystalline thread form inter-retinular fibres containing microtubules between retinula cells 1/2, 3/4, 5/6, and 7/1. The primary pigment cells are devoid of pigment granules, but are rich in rough endoplasmic reticulum. Proximal to each retinula cell nucleus are two basal bodies, one perpendicular to the other. The more proximal basal body extends two fibrillar feet proximally which fuse to form a horizontally-banded ciliary rootlet extending the length of the retinula peripheral to the rhabdom. Each ommatidium is surrounded by 16 secondary pigment cells. Interfacetal mechanoreceptors between some adjacent lenses each have a single bipolar neuron, with a dendritic sheath, tubular body, cilium, outer and inner sheath cells, and an axon surrounded by a neurilemma sheath cell.
Animals↗
Putative neurohemal areas in the peripheral nervous system of an insect, Gryllus bimaculatus, revealed by immunocytochemistry.
The morphology and position of putative neurohemal areas in the peripheral nervous system (ventral nerve cord and retrocerebral complex) of the cricket Gryllus bimaculatus are described. By using antisera to the amines dopamine, histamine, octopamine, and serotonin, and the neuropeptides crustacean cardioactive peptide, FMRFamide, leucokinin 1, and proctolin, an extensive system of varicose fibers has been detected throughout the nerves of all neuromeres, except for nerve 2 of the prothoracic ganglion. Immunoreactive varicose fibers occur mainly in a superficial position at the neurilemma, indicating neurosecretory storage and release of neuroactive compounds. The varicose fibers are projections from central or peripheral neurons that may extend over more than one segment. The peripheral fiber varicosities show segment-specific arrangements for each of the substances investigated. Immunoreactivity to histamine and octopamine is mainly found in the nerves of abdominal segments, whereas serotonin immunoreactivity is concentrated in subesophageal and terminal ganglion nerves. Immunoreactivity to FMRFamide and crustacean cardioactive peptide is widespread throughout all segments. Structures immunoreactive to leucokinin 1 are present in abdominal nerves, and proctolin immunostaining is found in the terminal ganglion and thoracic nerves. Codistribution of peripheral varicose fiber plexuses is regularly seen for amines and peptides, whereas the colocalization of substances in neurons has not been detected for any of the neuroactive compounds investigated. The varicose fiber system is regarded as complementary to the classical neurohemal organs.
Amines↗
Immunocytochemical localization of an insulin-like substance in the synganglion of the tick Ornithodoros parkeri (Acari: Argasidae).
Immunocytochemical staining based on the peroxidase-antiperoxidase method has shown that some neurosecretory cells (NSC) in the synganglion of the adult female tick Ornithodoros parkeri react with an antibody to bovine insulin. There are 18 regions of paraldehyde fuchsin-positive NSC of which three regions showed specific insulin-like immunoreactivity: anterolateral cheliceral, anteromedial stomodeal and posterior opisthosomal. Immunoreactivity can also be found in the extracellular surface of the neurilemma of the synganglion. This suggests a possible neurohemal site and release of neurohormone in a diffuse manner.
Animals↗
Demonstration of Fc gamma receptors on human peripheral nerve fibres.
Cryostat sections of human peripheral nerves adsorbed sheep erythrocytes (E) sensitized with rabbit IgG antibodies (A) (EA). No adsorption occurred using unsensitized E or E sensitized with IgM or F(ab')2 fragments of IgG. The binding of EA was inhibited by IgG and by Fc fragments of IgG, but not by IgA, IgM, F(ab')2 fragments of IgG or albumin, and thus indicated the presence of receptors for the Fc part of IgG (FcR). IgG1 and IgG3 subclasses inhibited the haemadsorption. IgG4 was less inhibitory than IgG1 and IgG3, whereas IgG2 did not inhibit the binding of EA. The receptors were sensitive to periodic acid, formaldehyde and heat. FcR were present in sections of both unmyelinated and myelinated nerves, indicating that the binding was not to myelin. FcR were also demonstrated in sections of fetal peripheral nerves at a gestational age of approximately 21 weeks. Using immune complexes of horseradish peroxidase (HRP) and rabbit IgG antibodies to HRP and a monoclonal antibody against FcR, the receptors were shown on the nerve fibres, probably on the Schwann cell membrane (neurilemma).
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Giant axonal neuropathy and leukodystrophy.
An 11-year-old Persian boy, born to consanguineous parents, manifested a progressive gait abnormality beginning at 5 years of age. A severe cerebellar disorder developed with associated dysfunction of the peripheral nervous system, but no sign of mental impairment. The sensory and motor nerve conduction velocities were greatly reduced, especially in the lower extremities. Cerebrospinal fluid protein was normal. Computed tomography and magnetic resonance imaging revealed leukoencephalopathy, especially in the cerebellum, but also in periventricular areas. The diagnosis of giant axonal neuropathy was established by biopsy of the sural nerve. The few previous histologic examinations have documented hyperplasia of the microfibrils which accumulate in the axons as well as in neurilemma, endothelial, and perineural cells. This is the first report of involvement of supraspinal portions of the central nervous system documented by postmortem examination after in vivo imaging methods corroborated the morphologic concomitants of the clinical symptoms.
Axons↗
Electrical activity in the chemoreceptors of the blowfly. II. Responses to electrical stimulation.
An analysis of the various parts of the electrical responses to the chemical and electrical stimulation of a single labellar chemosensory hair of the blowfly, Phormia regina, indicates that the recording conditions for the spike potentials approximate the intracellular recordings made in other types of sense cells. The large positive resting potential probably arises from the basement membrane of the hypodermal cells and neurilemma rather than from the neurons at the base of the chemosensory hair. The responses to polarizing currents passed through single chemosensory hairs support this analysis. The behavioral responses to similar polarizing currents are shown to result from the action of the current on the neurons at the bases of the adjacent chemosensory hairs. The reported neural interaction of the two chemosensory neurons associated with the chemosensory hair is probably due to the physical-chemical attributes of the stimulating solution rather than to any real neural interaction. Observations on the latency of the initial nerve impulse in response to chemical stimulation indicate that the chemosensory neurons are normally free from spontaneous spike activity.
Action Potentials↗
New perspectives on the role of autonomic nervous system in the genesis of arrhythmias.
Sudden cardiac death (SCD) is a major cause of morbidity and mortality in patients with coronary artery diseases and myocardial infarction (MI). There is a circadian variation of the frequency of SCD. Beta-blocker therapy significantly reduces the incidence of SCD after MI. These clinical observations suggest a close association between ventricular arrhythmia and sympathetic activity in patients with MI. Following injury, peripheral nerves undergo Wallerian degeneration, which may be followed by neurilemma cell proliferation and axonal regeneration (nerve sprouting), resulting in sympathetic hyperinnervation. It is possible that the increased innervation after myocardial injury may result in increased sympathetic nerve density, which in turn increases the propensity for cardiac arrhythmia. While this Nerve Sprouting Hypothesis seemed to be intuitive, there was no experimental proof of a causal link between sympathetic nerve sprouting and arrhythmogenesis. We therefore performed several studies to determine the relationship between nerve sprouting and cardiac arrhythmia. We also performed direct sympathetic nerve recording in an animal model of SCD. We found that cardiac sympathetic nerves are highly plastic. In addition to MI and rapid pacing, nerve sprouting and heterogeneous sympathetic hyperinnervation may also be induced by radiofrequency ablation, hypercholesterolemia, and stem cells transplantation. The coexistence of denervated and hyperinnervated area in the diseased myocardium could result in increased electrophysiological heterogeneity during sympathetic activation, leading to ventricular arrhythmia and SCD.
Animals↗