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At least 19 recordsLinked to original sources
PubMed1997

Machine milking of Ostfriesian and Lacaune dairy sheep: udder anatomy, milk ejection and milking characteristics.

Mammary cistern anatomy derived from ultrasound measurement, milk ejection in response to exogenous oxytocin and oxytocin release and milking characteristics with and without manual prestimulation in early (months 2-4) and late (months 5-8) lactation were investigated in Lacaune and Ostfriesian dairy ewes. Vertical ultrasound cisternal cross sections of the cisternal cavities did not differ in the two breeds, whereas the cisternal area fraction located lower than the exit into the teat channel was larger in Ostfriesian than in Lacaune sheep. The cisternal area enlarged within 1 min in response to i.v. oxytocin injection, indicating milk ejection. During milking, oxytocin concentrations in Lacaune generally increased dramatically within 0.5 min from the start of prestimulation or milking, whereas only slight or no oxytocin release was detected during milking without prestimulation in Ostfriesian ewes. Prestimulation induced oxytocin release in Ostfriesian sheep within 1-2 min after the start of milking, indicating delayed response to stimulation. Two peaked milk flow curves were observed when oxytocin release and milk ejection occurred only after removal of cisternal milk. This type of milk flow was more frequent in Ostfriesian than in Lacaune ewes and was reduced in both breeds by prestimulation, while the frequency of one peaked milk flow curves increased. During the course of lactation, milk yield, main milk fraction and milk flow rates decreased, while stripping yield was almost unchanged. Although milk yield was similar in both breeds, milk flow was lower and stripping yield was higher in Ostfriesian than in Lacaune ewes. We conclude that milk ejection in ewes occurred in response to elevated oxytocin concentrations. In Ostfriesian ewes reduced and delayed oxytocin response to teat stimulation resulted in milk ejection only during stripping. Therefore, and probably because a larger volume of the cistern was located below the teat exit in the Ostfriesian breed, stripping yield was higher in Ostfriesian than in Lacaune sheep.

Animals↗
PubMed1996

Simultaneous recording of oxytocin release, milk ejection and milk flow during milking of dairy cows with and without prestimulation.

The effects of manual teat stimulation compared with stimulation by the liner of a conventional milking machine and the influence of milk ejection on the course of milk flow during the entire milking were investigated. Twelve cows were milked with or without prestimulation on three quarters and milk flow was recorded while intramammary pressure (IMP) was recorded in the left front quarter and blood samples for the determination of oxytocin (OT) were taken at 1 min intervals. OT concentrations increased very similarly in response to manual teat stimulation or liner stimulation and remained elevated during the entire milking procedure. Milk flow curves were generally bimodal without stimulation and not bimodal during milking after prestimulation. Milk yield was not significantly lower in milkings without than with stimulation, whereas machine-on time was prolonged and peak milk flow rate was reduced during milking without stimulation. Time to reach milk flow plateau, time to reach peak flow rate and IMP profiles were similar in both treatments if time was calculated from the start of stimulation. IMP increased similarly in response to manual or liner teat stimulation. In milkings with and without prestimulation IMP did not reach its maximum until the start of milking. In bimodal milk flow curves the second increase of milk flow occurred concomitantly with the start of IMP increase; ejection IMP generally occurred concomitantly with the milk flow plateau. In some cases during milking without prestimulation only small and transient CT release was observed. In response, IMP did not increase to its maximum and increased further in response to a stripping-related CT release. During the course of milking IMP decreased slightly because of the emptying of the three milked quarters. After the end of milking, and with CT concentrations decreasing to the premilking values, IMP again decreased slightly to another plateau, probably owing to myoepithelial relaxation. Thus the course of milk ejection, i.e. the IMP profile, influenced milk removal markedly during early milking.

Animals↗
PubMed1976

[Milk ejection in goats and its influencing by feed].

By means of an experimental i.v. and i.m. oxytocin administration the quantitative relation between the amount of oxytocin and the quantity of ejected milk in goats was confirmed. With increasing oxytocin doses the latency period after the i.v. administration shortened, from 0.33 min. after 50 mU to 0.21 min. following 1000 mU. After the 2000 mU dose the latency period did not substantially change any more (0.18 min). The situation was nevertheless different after the i.m. oxytocin administration. No ejection followed after the 50 mU dose. Beginning with the 500 mU dose the latency period shortened, from 2.91 min. to 1.30 min. after the 2000 mU dose. With both administration manners the ejection time was prolonged up to the 1000 mU dose, then it did not substantially change. Similarly also the amount of ejected milk increased with the two administration methods up to 1000 mU, amounting to 20.6% after the i.v. and to 17.9% after i.m. administration. After 2000 mU the amount of milk ejected failed to substantially change, amounting to 19.4% with both administration manners. In experiments with manual udder stimulation with respect to feeding, the stimuli during feeding were found to influence the course of the milk ejection reflex. As compared with the pre-feeding experiments, the latency period after feeding was prolonged from 0.83 min. to 1.49 min; the amount of alveolar milk expressed in per cent of the total milk yield obtained decreased from 18.9 to 7.9%, and the ejection time shortened from 2.29 to 0.70 min. In the study the effect of various feeds on the alveolar milk ejection was also followed. Water administration did not call forth dropping off of the milk. Feeding hay called forth the ejection of 2.5% of alveolar milk occurring after an 0.25 min. latency period. Pollard provoked the ejection of 3% and the ejection time shortened from 2.29 toats (20%) can at least partially be ascribed to milk release during food intake.

Animals↗
PubMed1997

The milk-ejection reflex in the peri-partum rat: effects of oestradiol and progesterone on basal milk-ejection frequency and the facilitatory response to central oxytocin.

Experiments were undertaken to examine the effects of ovarian steroids on the functional characteristics of the milk-ejection reflex during late pregnancy. Basal milk-ejection frequency and response to i.c.v. oxytocin (OT) were compared in different experimental groups, using intramammary pressure recordings obtained in suckling tests under urethane anaesthesia. Ovariectomy (OVX) on day 20 of pregnancy significantly (P < 0.05) increased milk-ejection frequency on day 22, compared with sham-OVXed animals. I.c.v. injection of 2.2 ng OT during suckling had no consistent effect on milk ejection in either of these groups. Pretreatment with oestradiol (5 micrograms per day, s.c.) or progesterone (5 mg per day, s.c.) both resulted in a fall in milk-ejection frequency compared to oil-treated OVXed controls. However, whereas oestradiol-treated OVXed rats showed a facilitatory response to i.c.v. OT, with a significant (P < 0.05) increase in milk-ejection frequency in the 20 min period after injection, progesterone-treated OVXed rats showed only a delayed decrease in milk-ejection frequency (significant at P < 0.05 between 20-40 min after injection). Oil-treated OVXed rats showed no significant response to i.c.v. OT at any stage. Electrophysiological recordings from supraoptic OT neurones confirmed that bursting activity was increased by i.c.v. injection of OT in oestradiol-treated, but not progesterone-treated rats. Further experiments with hysterectomized ovariectomized rats indicated that the difference in response to i.c.v. OT in oestradiol- vs progesterone-treated rats was not related to changes in the timing of birth induced steroid treatments. These findings demonstrate the ability of ovarian steroids to alter the characteristics of the milk-ejection reflex in the peri-partum rat. In particular, the rise in oestradiol and fall in progestesterone near term, may contribute to programming of the facilitary response to central OT in preparation for lactation.

Animals↗
PubMed1987

The subfornical organ and relaxin-induced inhibition of reflex milk ejection in lactating rats.

Experiments were carried out on anaesthetized, lactating rats to investigate the possible role of the subfornical organ in mediating relaxin-induced inhibition of reflex milk ejection. Milk ejection was judged by the behavioural response of the sucklings and by transient rises in intramammary pressure. Radiofrequency lesions of the subfornical organ, or control lesions in adjacent areas of the cerebrum, did not affect the pattern or the magnitude of intramammary pressure changes at reflex milk ejection. Purified porcine relaxin given by either i.v. (5 micrograms) or intracerebroventricular (50 ng) injection suppressed reflex milk ejection in intact, sham-lesioned and control-lesioned rats, but had no effect on either the pattern or magnitude of reflex milk ejection in rats with lesions of the subfornical organ. The subfornical organ, which is situated at the interface between the blood, brain and the cerebrospinal fluid appears to mediate, at least in part, the relaxin-induced inhibition of reflex milk ejection in the rat.

Animals↗
PubMed1979

Inhibitory effect of adrenaline on oxytocin release in the ewe during the milk-ejection reflex.

Milk-ejection activity was determined in the blood plasma of ewes during normal milking and during milking when adrenaline was injected intravenously before or after udder stimulation. It was found that administration of adrenaline either before or after udder washing, decreased the oxytocin concentration and milk yield but increased the yield by hand-stripping. Adrenaline also retards the average time for peak oxytocin concentration. These results and the use of a beta-receptor blocker to inhibit the effect of adrenaline at the myoepithelial cell level indicate that in ewes adrenaline can prevent the release of oxytocin from neurohypophysis.

Animals↗
PubMed1994

[The role of vacuum and tactile stimuli during milk ejection from the human breast].

Milk ejection in lactating women was performed by breast pump with vacuum and tactile (touch-compression) components. It was found that milk flow rate changed in a pulsatile manner at about 1-2 min interval and peaks milk flow coincided with peaks intramammary. Switch off the tactile component increased the latency of milk ejection 1.5-2 fold and decreased total flow rate 2 fold. It was concluded that touch-compressions stimuli necessary for generation milk ejection reflex and milk ejection from the women mammary gland.

Breast↗
PubMed1982

The milk ejection reflex in the pig.

1. The milk ejection reflex in response to suckling was studied in conscious sows by continuous recording of intramammary pressure, radioimmunoassay of plasma concentrations of neurohypophysial hormones, and observation of the behaviour of the sows and piglets.2. A regular pattern of nursing, suckling and milk ejection was observed. The mean duration of the suckling period was 6.3 min. Over 144 suckling periods, 113 milk ejections were recorded. Each milk ejection was characterized by a sudden rise in intramammary pressure reaching 20-49 mmHg, and lasting 8-41 sec. Milk ejections occurred only once per suckling period, at a mean interval of 44.3 min.3. Each milk ejection occurred with a mean latency of 2.4 min from the onset of a period of initial massage of the udders by the piglets, and was coincident with a period of quiet suckling when the piglets were consuming milk. The onset of nursing was signalled by the sows grunting in a rhythmic manner. In most cases, the frequency of grunts, at first low, increased suddenly 23 sec before milk ejection.4. During eighteen suckling periods leading to milk ejection, neurohypophysial hormone assays performed on serial blood samples showed an increase in plasma concentration of oxytocin up to 30 sec before milk ejection. The concentration of lysine-vasopressin did not rise above basal levels.5. In 21.4% of the suckling periods, no rise in intramammary pressure was observed. In these ;incomplete sucklings', the sow usually failed to grunt rapidly, and the piglets obtained no milk. For three of these periods, hormone assay showed no increase in oxytocin or vasopressin concentrations in blood.6. Oxytocin given intravenously produced variations in intramammary pressure which depended on the dose and the rate of injection. Rapid injections of 25-50 m-u. oxytocin, caused milk ejections similar to those induced by suckling. When oxytocin was administered at different rates, the faster the injection, the shorter the latency and the higher the amplitude of the response. Plasma concentrations of oxytocin after injection of 25 m-u. were similar to those observed during reflex milk ejection.7. Trains of electrical pulses were applied to the posterior pituitary of four anaesthetized sows. At frequencies of stimulation above 10 Hz, a rise in intramammary pressure and an increase in plasma oxytocin and vasopressin concentrations were observed. At frequencies of stimulation of 30-50 Hz, the response of the mammary gland and the time course of the variations in oxytocin plasma concentrations were similar to those observed during natural reflex milk ejection.8. It is concluded that reflex milk ejections during suckling in the pig are caused by the intermittent and spurt-like release of about 25 m-u. oxytocin, without concomitant vasopressin release. It is postulated that the release of oxytocin is probably precipitated by a brief and massive activation of oxytocin-secreting neurones in the hypothalamus. Central mechanisms controlling the intermittent release of oxytocin are discussed.

Animals↗
PubMed1992

Aetiology of disturbed milk ejection in parturient primiparous cows.

Milk flow in nine primiparous cows with disturbed milk ejection (D) and in six corresponding control animals (C) with normal milk removal was recorded during machine milking and blood samples were taken before and during milking to determine plasma oxytocin, vasopressin, prolactin, cortisol, oestradiol-17 beta, luteinizing hormone, progesterone and beta-endorphin concentrations. Manual teat stimulation before milking lasted for 1 min. After milk flow had stopped, air was blown into the vagina for 2 min. When milk flow had stopped again, 1 i.u. oxytocin and finally 10 i.u. oxytocin were injected to remove residual milk. During and after teat stimulation, oxytocin remained basal in D, but increased in C, whereas prolactin increased in both groups. While 94% of total milk was obtained in C during this period, only 9% could be removed from D, indicating lack of alveolar milk ejection. During vaginal stimulation, oxytocin increased transiently in D and more than by teat stimulation in C. This allowed the removal of 75% of milk in D, whereas almost no more milk was available in C. After oxytocin injections, 3 and 16% of residual milk were obtained in C and D respectively. Basal oestradiol-17 beta concentration was higher in D than in C (11.6 and 2.0 ng/l respectively), whereas beta-endorphin level was lower (24.1 and 86.6 micrograms/l respectively). Basal concentration of luteinizing hormone and progesterone, and concentration of cortisol and vasopressin before and during milking were comparable in C and D. We conclude that in cows with disturbed milk ejection afferent nervous pathways to the hypothalamus were intact, because prolactin was released by teat stimulation. However, oxytocin was only released by vaginal stimulation, i.e. milk ejection was centrally inhibited during teat stimulation.

Animals↗
PubMed2005

Normal and disturbed milk ejection in dairy cows.

More than 80% of the milk stored in the udder, i.e. the alveolar fraction, only available after milk ejection is induced by the release of oxytocin and myoepithelial contraction. Milk ejection is induced by tactile teat stimulation, either manually or by the milking machine. The time from the start of a tactile stimulation until the occurrence of milk ejection spans from 40 s up to more than 2 min and increases with decreasing degree of udder filling. Therefore, cows need a longer pre-stimulation in late stages of lactation or if the milking is performed shortly after the previous milking whereas in full udders, pre-stimulation is less important. Milk ejection can be disturbed under several conditions, for example, during milking in unfamiliar surroundings or for several weeks after parturition in many primiparous cows. We found that a disturbed milk ejection is due to a reduced release or complete absence of oxytocin from the pituitary. During milkings with disturbed milk ejection in unfamiliar surroundings, the concentrations of cortisol and beta-endorphin were elevated. While exogenous glucocorticoids could not induce the disturbance, morphine caused inhibition of oxytocin release during milking. However, spontaneously occurring disturbance of milk ejection could not be abolished by the opioid-antagonist naloxone. On the other hand, severity of disturbed milk ejection and coping capacity towards novel milking environment was inversely related to the cortisol release in response to ACTH, i.e. adrenal cortex activity. Therefore, the susceptibility of individual cows to the inhibition of oxytocin release and milk ejection could be predicted by an ACTH challenge test.

Animals↗
PubMed2004

Ultrasound imaging of milk ejection in the breast of lactating women.

OBJECTIVE: Currently, the methods for assessing milk ejection in women include serial sampling of plasma oxytocin and measurement of intraductal pressure, both of which are invasive and may induce stress. We hypothesized that milk ejection would cause an increase in milk-duct diameter that could be observed noninvasively with ultrasound, and this could be used to investigate the physiology of milk ejection in women. METHODS: One milk duct was scanned in the unsuckled breast in 2 groups of mothers: group BB (n = 21) for the beginning of a breastfeed and group EB (n = 24) for the entire breastfeed. A duct also was monitored for a 5-minute period on 2 separate days in the absence of factors that may induce milk ejection in group EB to provide a baseline duct diameter. Milk intake at a breastfeed was measured by test weighing. RESULTS: A significant increase in milk-duct diameter was observed when milk ejection was sensed and/or the infant changed its swallowing pattern in both groups. Multiple increases and decreases (mean: 2.5 per breastfeed; standard deviation: 1.5; n = 62) in duct diameter occurred in group EB. Duct diameter remained relatively stable between breastfeeds (coefficient of variation: 1.4%-8.3%). Infant milk intake was positively related to the number of milk ejections (r2 =.365; n = 57). CONCLUSIONS: Ultrasound is an objective, noninvasive technique for detecting milk ejection by observing an increase in milk-duct diameter. However, this technique requires an experienced ultrasonographer, adequate imaging time, and surroundings conducive to breastfeeding. Multiple milk ejections were common during breastfeeding, although they were not sensed by mothers. The number of milk ejections influenced the amount of milk the infant consumed.

Adult↗
PubMed2001

Milk ejection in dairy cows at different degrees of udder filling.

Occurrence of milk ejection and course of milk removal were investigated in 18 dairy cows at milking intervals of 4, 8 and 12 h in early, mid or late lactation. Milk ejection occurred fastest in early lactation at a milking interval of 12 h and was delayed at short milking intervals and in late lactation. Storage capacity of the udder was estimated and the actual milk yields of experimental milkings were calculated as a percentage of storage capacity, i.e. degree of udder filling. It was shown that the occurrence of milk ejection after the start of teat stimulation is a function of udder filling. The relationship between the degree of udder filling and the delay from the start of milking until commencement of milk ejection followed a linear regression curve. Changes in occurrence and course of milk ejection have to be considered in practical milking, mainly in late stages of lactation and after short milking intervals. In automatic milking systems where variable and sometimes extremely short milking intervals occur, the duration of pre-milking udder preparation should be adapted to the expected milk yield at each individual milking procedure.

Animals↗