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At least 19 recordsLinked to original sources

Serological characterization of group-A streptococci associated with skin sepsis in meat handlers.

A series of outbreaks of skin sepsis among meat handlers in England during 1974 and 1975 afforded an opportunity to study the group-A streptococci commonly isolated from the lesions. Few of these streptococci could be M typed with existing antisera. Intensive study of strains from two outbreaks of sepsis in one abattoir in Shropshire led to the recognition of three new provisional M types. The streptococci were first sorted according to T-typing pattern and ability to produce opacity factor. Opacity-factor producing strains with the same T pattern were then screened for inhibition of opacity production by the sera of convalescents from the same outbreak. Finally, M antisera were made in rabbits against representative cultures. Sera against the three new provisional types were used to re-examine streptococci from 20 other outbreaks or incidents of sporadic infection among meat handlers. This increased the proportion of typable strains from 3% to 55%. Two of the new provisional types (nos. 2015 and 1658; both T25/Imp 19, opacity-factor positive) were confined to the Shropshire outbreak, but the third (no. 2681; T14, opacity-factor negative) was found among strains from meat workers in eight other geographically distinct areas. In all, 31% of 131 distinct strains from meat workers, but less than 1% of 2816 strains from other British sources, belonged to provisional type 2681. Thus, in Britain, one M-type of group-A streptococcus appears at present to be almost exclusively associated with sepsis in meat workers.

Abattoirs

Microbiology of bruised tissue.

No significant differences could be found in the microbial quality of bruised and unbruised tissue provided that the two types of tissue were treated identically. This suggests that there is no good reason for the condemnation of bruised tissue, which could well be used in manufactured products.

Animals

Antibody to Escherichia coli enterotoxin in meat-packing workers.

Meat-packing plant employees exposed to raw animal products had serological evidence of higher infection rates with heat-labile toxin producing enterotoxigenic Escherichia coli (LT-EEC). In those employees with multiple sera available for study over a ten-year period, a drop in mean anti-LT-EEC titer was observed, suggesting altered ecology of or exposure to the organism during this time. Prospective studies need to be done to determine if meat-packing workers actually experience a greater incidence of LT-EEC-induced diarrheal disease.

Adult

[Professional allergic diseases in CSSR (author's transl)].

The authors have watched more than 300 workers of various fodder manufactures in allergological, immunological and clinical way. The findings have been evaluated to the part of the exposition, hygiene and professional allergens. The symptoms of the latent sensitisation so as clinical symptoms after longer time of employment give evidence about a great importance and higher biological activity of the most fodders of modern farming and about new possibilities of the professional allergisation.

Animal Feed

Microbiological investigation of meat wholesale premises and beef carcases in Johannesburg.

Microbiological surveillance by swabbing meat wholesaler premises revealed ineffective cleaning and build-up of bacteria. Proper cleaning, sanitation and handling resulted in a vast improvement during 1975-77. Beef samples from the neck of carcases in the wholesale trade were investigated by microbiological methods. Excessive total bacterial counts were obtained from numerous carcases. Most carcases carried coliform organisms. Roughly 90% of carcases were contaminated with E. coli I; counts exceeded 10(3)/g in 18% of carcases tested. Twenty serotypes of Salmonella were identified. Salmonella contamination decreased from nearly 5% in 1975 to less than 0,5% in 1977, and S. aureus contamination from 52% to 36% during the same period. Approximately 30% of carcases revealed contamination with unidentified clostridial species. The results indicate the need for stricter control over the production and slaughter of animals and over the handling of carcases in the wholesale trade.

Animals

[Epidemiological studies on salmonella in a particular area ("Walcheren Project"). III. The incidence of salmonella in man, insects, gulls as well as foods scrapings from butcher's blocks, effluents of sewage treatment plants and drains from butcher's shops (author's transl)].

In continuation of previous studies, various materials (meat and meat products, insects, gull droppings, scrapings from butcher's blocks, effluents of sewage treatment plants, drains from butcher's shops and faeces of patients) were examined again at the same time for the presence of Salmonella in a relatively small are (Walcheren) over a period of three months. As was also the case in previous studies, S. typhi murium (27.5 per cent), S. panama (22.2 per cent) and S. brandenburg (9.2 per cent) were the three serotypes most frequently isolated. The three most frequently isolated phage types of S. typhi murium were II 505 (62.1 per cent), II 502 (5.3 per cent) and I 650 (4.2 per cent). The serotypes and phage types were present in nearly all the materials studied which again emphasizes the fact that there are contamination cycles of Salmonella. These studies showed that the route of contamination divides in the butcher's shop. Salmonella ogranisms carried with the meat frome the slaughter-house find their way into the drains on the one hand, and, by meat and meat products, to consumers on the other. Moreover, the high degree of contamination of effluents is not in accordance with the small number of cases of salmonellosis.

Animal Feed

[Assessment of bacterial counts in the meat-processing industry. I. A review of the literature (author's transl)].

Frequent examination of large numbers of samples of the surface of carcasses and apparatus is essential to gain a clear insight into the hygiene of the meat-processing line. To make this possible, efforts should be made te reduce the number of sampling sites of each carcass as well as the number of groups of bacteria to the studied, and to save time and material. Methods so far developed to the assessment of bacterial counts are reviewed in the present paper. It is concluded that the spray-pistol method, the Stomacher method, the drop method, the membrane filter method, microcolony counts and the spiral method are particularly eligible for closer examination. The methods referred to should be tested by the following criteria: (1) simplicity; (2) reliability and reproducibility and (3) saving of time and material.

Bacteriological Techniques

[Cross-infection during scalding and plucking of broiler chickens (author's transl)].

In two poultry-processing plants, experiments were carried out to detect cross-infection during scalding and plucking of broiler chickens. These trials showed that cross-infection was only detectable when experimental infection with the indicator micro-organism Escherichia coli K12 was applied externally to the broiler chickens. When infection was applied internally, only a small degree of transmission was observed. Indicator micro-organisms applied externally to broiler chickens prior to scalding and plucking, were present on the cooled product in larger numbers than those applied internally in the intestines of broilers. The number of carcasses which were positive after cooling was found to have decreased in poultry-processing plant B compared with the situation after plucking, whereas this number was not affected to any appreciable extent in processing plant A.

Animals