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[Sclerosis of the intestinal lymphatic vessels].

Fibrosis of the intestinal lymphatic vessels, produced in one case by tuberculosis and, in the other, by appendicitis and peritonitis, caused blockage of the main lymphatic vessels causing, clinically, a protein-losing enteropathy similar to that noted in congenital lymphatic diseases of childhood. In the laboratory, there was noted a fall in serum protein, lipid and cholesterol. A fat absorption test was very abnormal showing a flat curve. During laparotomy, there was discovered on the small intestine, the same layout of lymph vessels, resembling a lace network, as that observed in congenital malformations. Intestinal lymphography showed considerable stasis of the opaque substance and absence of injection of the lymph vessels in the mesentery.

Chylous Ascites

Periodic acid methenamine silver (pam) staining of the human subcutaneous lymphatic vessel.

The fine structure of the subcutaneous lymphatic vessel taken from the dorsum pedis of the human adult was investigated without and after impregnation by periodic acid methenamine silver (PAM). To check the stainability of another tissue, the renal glomerulus of the rat was also examined in the same way as outlined above. The membrane system of various cells stains positive. No special surface coat of the endothelial cell of the lymphatic vessel was detected. The fine intracellular filament as well as a continuous basal lamina of the endothelial cell were almost PAM-negative, although a part of the basal lamina was occasionally weakly positive. The chemical composition of the basal lamina of the lymphatic vessel is considered to differ from that of the glomerular blood capillary of the kidney, which is PAM-positive. This difference is thought to be due to the smaller polysaccharide content in the former than in the latter.

Adult

Lymphatic vessels of healthy and inflamed gingiva.

Lymphatic vessels were traced in healthy and early inflamed gingivae of various nonhuman primates. Lymphatic capillaries and primary collecting vessels originated in the connective tissue papillae and stroma near or adjacent to the attachment epithelium. They emptied into thin-walled irregular-shaped collecting vessels that drained into the periodontal ligament. In early inflammation, all the lymphatic vessels of luminal caliber were distended within and/or surrounding the inflammatory infiltrate.

Animals

[Anatomical variants of the lymphatic vessels connecting the inguinal lymph nodes].

The study of anatomical variants of lymphatic vessels connecting inguinal lymph nodes was carried out on 56 corpses of adult persons of both sex whose deaths were not connected with lesions in the lymphatic system of the pelvis and lower extremities. The inguinal lymph nodes and their afferent and efferent lymphatic vessels were detected by the method of intradermal injection and by the method of direct injection into the lymphatic vessels. It was stated that groups of the inguinal lymph nodes, as well as the nodes in every group determined, can serve as nodes of different stages for afferent lymphatic vessels running from different parts of the body and organs.

Female

Lymphatic vessels of the mammalian heart.

An in situ heart lung preparation was developed to label lymphatics of the actively beating dog heart with subsequent fixation by vascular perfusion. Immediately after interstitial injections of trypan blue and colloidal carbon, a rich plexus of lymphatic vessels was visualized in the epicardium of the actively beating heart. With this method of fixation, tissue preservation is generally excellent and uniform throughout the heart. In thin sections examined with the electron microscope, lymphatic vessels are easily recognized by the content of plasma proteins which is preserved as an electron dense precipitate that is evenly dispersed throughout the lumen. An extensive plexus of thin walled lymphatic vessels is observed throughout the epicardial, myocardial and subendocardial regions. Numerous anchoring filaments are observed closely apposed to the abluminal endothelial surface which extend into the surrounding connective tissue. The distribution and ultrastructure of the cardiac lymphatic vessels are discussed in relation to their role in the removal of interstitial fluid from the heart.

Animals

Scanning electron microscopy of collecting lymphatic vessels and their comparison to arteries and veins.

The morphology of canine thoracic duct and peripheral collecting lymphatics was determined using light microscopy together with scanning and transmission electron microscopy (SEM and TEM). The thoracic duct was compared to the thoracic aorta and to the vena cava. Luminal surface detail was determined using the secondary imaging mode of the SEM. Subsurface nuclear and connective tissue detail was determined using back-scattered electron imagining combined with Willard's modification of Gomori's Methenamine Silver Stain. Central and peripheral lymphatic vessels have surface morphology distinct from either arteries or veins. The endothelial cell density in lymphatic vessels is less than in arteries or veins. The nuclear chromatin of lymphatic endothelial cells is coarsely granular and evenly distributed. This contrasts with nuclei from arteries or veins in which the chromatin is segmented. The distribution and orientation of lymphatic subsurface connective tissue fibers also differs from that seen in arteries and veins. It is concluded that canine lymphatic vessels have a unique surface and subsurface morphology and can be unequivocally identified by SEM.

Animals

[The lymphatic vessels of the stomach during phylogenesis and ontogenesis (author's transl)].

The stomach lymphatic channel of all classes of vertebrata representatives beginning from cartilagnous fish, was discovered. The lymphatic channel of cartilaginous and bony fish is presented only with lymphatic capillaries. The lymph is removed along peri- and paravascular lymphatic capillaries. The channel of the stomach of amphibians and reptiles is differentiated in lymphatic capillaries and lymphatic small trunks, while in birds and mammalia it is differentiated in lymphatie capillaries and lymphatic vessels. In human beings, macacus, dogs and cats the removing lymph pathways of the stomach wall in ontogeny undergo the following stages: 1) a lymphatic capillary, 2) a "wide" lymphatic capillary and 3) a lymphatic vessel. These stages are considered to be recapitulation of the developing process of the removing lymph pathways in vertebrate phylogenesis. The stomach lymphatic channel in vertebrate species examined is lined with a continous layer of endothelium cells.

Anatomy, Comparative

Lymphatic vessels of the human dental pulp.

Noncarious teeth obtained from individuals 15 to 50 years of age were used to study the lymphatic drainage of the human pulp. Thick sections (50 to 150 microns) were stained with iron hematoxylin for the demonstration of lymph and blood vessels. Lymph capillaries originated as blind sacs in the odontoblastic layer and in the pulp proper near the pulpo-odontoblastic border. They drained into small thin-walled collecting vessels that were irregular in shape and showed great variability in their drainage patterns. Communications between these vessels were very common. The larger conducting lymphatic vessels accompanied the blood vessels and nerves in their course through the pulp. They could be identified by their thin walls and small size. The large caliber lymphatic vessels contained valves, a structure not present in the veins of the same size. The conducting lymphatic vessels passed through the roots as individual units without draining into a large single vessel. The lymphatic vessels of the human pulp must be considered as a pathway for the removal of excessive tissue fluid in normal and diseased pulps.

Adolescent

[Our experience with methods of studying the visceral lymphatic vessels].

The authors relate their experience on the investigation of the visceral lymphatic vessels. Their experience concerns the lung, the pancreas and essentially the stomach. This work has been carried out as well on the corpse using the technique of Papamiltiades as on the living. On the corps, only descriptive and topographic results may be taken into account; actually, to consider drainage in such a case seems to be a misinterpretation considering absence of lymphatic circulation and the importance of technique on the results. Only confrontation with results in vivo allows an interpretation.

Humans

Papillary endothelial proliferation in cystic lymphangiomas. A lymphatic vessel counterpart of Masson's vegetant intravascular hemangioendothelioma.

Papillary endothelial proliferation similar to that of Masson's "vegetant intravascular hemangioendothelioma" of the blood vessels was observed in two cases of cystic lymphangiomas. They are believed to be the lymphatic vessel counterpart of Masson's entity. Twenty-five other specimens of various lymphangiomas examined (18 patients) did not contain the same change. As was emphasized previously for Masson's lesions, lymphangiomas containing similar endothelial changes should also not be mistaken for malignant vascular tumors, since in these two cases, no unusual clinical course supervened. Although the histogenesis of the lesion as proposed by Masson has been disputed, both primary and secondary endothelial proliferation seem to remain possible in the formation of these lesions.

Cell Division

Morphological changes in lymphatic vessels in pulpal inflammation.

Noncarious, carious, and restored human teeth obtained from individuals 20 to 65 years of age were used to study the differences in lymphatic drainage between healthy and inflamed pulps. In carious teeth, the lymphatic vessels located within and/or surrounding the inflammatory lesion were distended, a situation not demonstrable in pulps of noncarious and restored teeth.

Adult

Observations on the deep lymphatic circulation of the limbs and on its function: (the deep lymphatic vessels in the post-phlebitic syndrome of the lower extremities).

In 60% of patients with post-phlebitic syndrome lymphography of the lower extremity shows involvement of the deep system. In 77% of the patients there was increase in the number and caliber of the deep lymphatic vessels. They frequently appear elongated, tortuous, and ectatic but usually do not show signs of insufficiency. It appears as if these vessels act as collaterals and compensate for difficiencies of the deep venous circulation. Multiple lympholymphatic and lymphovenous communications have been observed.

Adult

The fine structure of neoplastic invasion: invasion of liver, skeletal muscle and lymphatic vessels by the Rd/3 tumour.

Neoplastic invasion as seen in the rat Rd/3 neoplasm has been studied electron microscopically in three sites: abdominal wall, footpad and liver. This neoplasm invades by peripheral cell division and active cell migration. Neoplastic cells protrude many fine processes and compress host cells. Neoplastic cells invade lymphatic vessels in a way similar to that in which white blood cells penetrate them - by the protrusion of fine cytoplasmic processes and passage, sometimes in clumps through open cell junctions. There is no ultrastructural evidence of secretory phenomena.

Abdominal Muscles

[Lymphatic vessels in the uterus of aging nulliparous and multiparous mice].

The morphological changes in the uterine lymphatics in aging nulliparous and multiparous mice, as shown by the application of dyestuffs (patient blue violet and Japan ink) are described, and their appearance corresponds to that described in earlier papiers by Fabian (1976, 1977, 1978). It is apparent on the basis of these findings that with the cessation of the sexual cycle, the previous regularly repeated morphological changes in the mouses uterus move into another stage, and associated with this the lymphatics inevitably take part in the aging process. According to Lindner (1975) this is essentially a dynamic process, rather than one which is purely passive, dengerative or involutional.

Aging