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Results for “Liver Extracts”

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At least 19 recordsLinked to original sources
PubMed1989

[Metabolic studies of 5-ethyl-2'-deoxyuridine with liver extract, liver cells and liver cell culture].

5-Ethyl-2'-deoxyuridine (EtUdR) was converted with rat liver extract, rat liver cells and rat liver cell cultures into its metabolites 5-ethyluracil and 5-(1-hydroxyethyl) uracil. The analytical identification of the metabolites as well as the investigation of the biotransformation process was performed by thin layer chromatography, autoradiography and high pressure liquid chromatography.

Animals↗
PubMed1977

[Liver extract effect on liver microsomal system and on an experimental model of intoxication].

A lyophilized liver extract (FLR) lengthens hexobarbital action in control rats ; it decreases slightly the induction of hepatic microsomal enzymes and normalizes diphenylhydantoin protection against electroshock seizure in phenobarbital-treated animals ; in the event of a CC14 intoxication, this extract reestablishes values close to normal for mebubarbital metabolism in mice and for BSP clearance in rats. FLR could act in regularizing drug metabolism.

Animals↗
PubMed1977

In vitro inhibition of tritiated thymidine uptake in Morris hepatoma cells by normal rat liver extract: a possible liver chalone.

A phosphate-buffered saline extract of normal rat liver tissue almost completely inhibited DNA synthesis in Buffalo rat Morris hepatoma 7777 cells in vitro. This effect was tissue-specific because it did not occur with extracts of kidney, spleen, heart, lung, muscle, and hepatoma. The liver extracts did not inhibit in vitro human prostate carcinoma or phytohemagglutinin-stimulated human and rat peripheral blood lymphocyte cultures. The addition of 10% fetal calf serum in the incubation medium had no effect on this inhibition. We determined that doses of liver extract that inhibit thymidine incorporation were not toxic inasmuch as treated cells remained viable, as indicated by trypan blue exclusion. The liver extract may thus contain a cell-specific mitotic inhibitor, a chalone for hepatoma cells.

Animals↗
PubMed1987

Modulation of lymphocyte proliferation by murine liver extract.

Murine liver extract (LEx) purified by ammonium sulfate (45-70% saturation) possesses a strong inhibitory effect on human lymphocyte proliferation. We have shown that the inhibitory effect of LEx is not via a cytotoxic effect and that it is proportional to the length of incubation with LEx. Mitogen-prestimulated lymphocytes are more resistant to LEx inhibition than cells not prestimulated. B cells stimulated by PWM are more susceptible to LEx-induced inhibition than PHA- or Con A-stimulated T cells. In Con A cultures, there may be a population of cells more resistant to LEx inhibition. This population is not yet identified. The degree of reversibility of LEx inhibition was different in cells prestimulated by different mitogens. The inhibitory activity of LEx decreased in the presence of an increasing number of cells in the culture.

Animals↗
PubMed1981

Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts.

Rat and hamster liver extracts were found to contain DNA glycosylases capable of removing 3-methyladenine and 7-methylguanine from methylated DNA. The activity of 7-methylguanine-DNA glycosylase was greater tin hamster than in rat liver extracts. This finding is consistent with previous reports that the half-life of 7-methylguanine in DNA after treatment with the carcinogen dimethylnitrosamine is longer in rats than in hamsters. These enzymes may, therefore, play an important role in the removal of abnormal alkylation products from mammalian cell DNA. Rodent liver extracts also contained a DNA glycosylase able to remove from alkylated DNA the imidazole-ring-opened form of 7-methylguanine which is produced by treatment with alkali. Although this product may occur in vivo after treatment with alkylating agents to only a very small extent, the enzyme may be needed to minimize its potentially harmful biological effects.

Adenine↗
PubMed1969

A controlled trial of a protein-free liver extract in the treatment of chronic liver disease.

A double-blind trial of a protein-free liver extract (Ripason) which was administered both orally and intramuscularly is described in 20 patients with stable cirrhosis. Control and treated groups were similar in composition except for age. Both groups showed some symptomatic improvement during the trial. Fewer patients were recorded as worse on Ripason but no significant changes in body weight, haemoglobin, or liver function tests could be detected.

Adult↗
PubMed1990

[A comparative study of the action of liver extracts from C57Bl and CBA mice on hepatocyte adhesion].

The adhesiometric methods were used for the investigation of influence of the preparations extracted from liver of CBA mice, predisposed to spontaneous blastomogenesis, and stable C57BL mice on cell adhesion. The extraction was carried out at 4 degrees C or 20 degrees C under condition of Ca2(+)-deficiency, so four preparations were investigated. The differences in adhesive activity between these liver preparations of two mouse lines were determined. The influence of the liver extracts (20 degrees C) from C57BL and CBA mice on cell adhesion was different. These preparations from C57BL mice contained the cell surface and membrane connected adhesion factors. The liver extracts (4 degrees C) from C57BL mice contained the cell surface adhesion factors influenced adhesive properties of hepatocytes under condition of cell surface preservation, and acted only on hepatocytes of C57BL mice. Our results concluded that, first, there were different adhesion factors extracted at 4 degrees C and 20 degrees C in liver preparations from C57BL mice; second, there was the strong change in cell surface of CBA mouse hepatocytes.

Animals↗
PubMed1977

Effect of mercurascan and tetracycline on ischaemia-altered immunogenicity of liver extract alloantigens in mice.

The action of Mercurascan and Tetracycline on ischaemic liver in mice was investigated. The effect was reflected in different immunogenicity (in allotrasplantation reaction) of liver extracts 1 mg. membrane fraction) derived from treated and normal organs in the donor--recipient strain combination B10--B10.LP. In the recipients treated with a single administration of extract from ischaemic liver the survival time of skin grafts was shortened as compared to the untreated control group and the control group given normal liver extract. Immunogenicity of the liver extracts from Mercurascan- or Tetracycline-treated mice was diminished.

Animals↗
PubMed1975

[Inhibition of the incorporation of tritiated thymidine in fetal rat liver in cell culture by a fractioned liver extract].

A dialyzed rat liver extract has been chromatographed on "Sephadex G 25" and then on "Sephadex G 50". Thus we found a fraction which inhibits the incorporation of tritiated thymidine and stimulates the incorporation of labelled orotic acid into foetal rat liver cells in culture, but has no effect on DNA synthesis in cell lines derived from lymphocytes and fibroblasts.

Animals↗