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At least 19 recordsLinked to original sources

Parental care improves offspring survival and growth in burying beetles

Burying beetles (genus Nicrophorus) provide elaborate parental care to their offspring. Parental beetles defend a small vertebrate carcass, which constitutes the sole food source for the larvae. They also manipulate the carcass in various ways and directly regurgitate pre-digested carrion to the young. The benefits of carcass manipulation and regurgitation have been the subject of a few small-scale studies that have yielded conflicting results. In this study, we investigated the benefits of these behaviours and tested for possible beneficial effects on larval survival rates and final body mass in N. vespilloides. In this species: (1) larval survival and mass were significantly higher in broods receiving parental care throughout larval development on the carcass than in broods developing in the absence of adults; (2) parental presence immediately subsequent to larval hatching greatly improved larval survival rates; (3) continued parental presence for several days further improved larval growth, leading to a greater final mass of individual larvae; (4) larval survival and growth were improved by parental preparation of carcasses and by an excision made in the integument of the carcass surface by the parents that allows the larvae ready access to their food; (5) positive effects of parental feeding on larval survival and growth were not mediated by the transfer of symbionts. Copyright 1998 The Association for the Study of Animal Behaviour.

Journal Article↗

Integrative machine learning and transcriptomic analysis reveals molecular mechanisms underlying low survival rate in larval Chinese Bahaba (Bahaba taipingensis).

Chinese Bahaba (Bahaba taipingensis) is a Class I protected marine fish endemic to China. Low larvae survival during artificial breeding severely hinder population recovery. To investigate the molecular mechanism of high mortality in larval fish, this study performed RNA-seq on liver from naturally deceased (ND) and mass-dead (MD) individuals, combined with least absolute shrinkage and selection operator (LASSO) regression and random forest (RF) algorithms to screen for core signature genes. A total of 873 differentially expressed genes (DEGs) were identified, including 112 upregulated and 761 downregulated genes. GO and KEGG enrichment analyses revealed significant enrichment in amino acid metabolism disorders, one‑carbon folate pool impairment, PPAR signaling abnormalities, ECM-receptor interaction, focal adhesion pathway, indicating widespread metabolic suppression accompanied by extracellular matrix remodeling and signaling disturbances in the livers of MD fish. MAD pre-filtering combined with dual machine learning algorithms yielded 18 robust core signature genes, among which SLC38A4, MMP1, FADD, FKBP5, and APOB were consistently identified as high-frequency core genes by both algorithms. SLC38A4 exhibited the highest importance score in the RF model and was significantly downregulated, making it the primary molecule distinguishing ND from MD phenotypes. ROC curve analysis showed that both models achieved an AUC of 1.000 (95% CI lower bound: 0.610), confirming the precise discriminatory ability of the core genes. GSEA further demonstrated significant enrichment of this core gene set in ND samples. This study provides the first systematic elucidation of the molecular mechanisms underlying liver dysfunction in low survival rate B. taipingensis, characterized by amino acid transport impairment, metabolic reprogramming, and structural remodeling, offering theoretical foundations for health assessment, early mortality risk warning, and artificial breeding conservation of this species.

Animals↗

Effect of ivermectin on survival and fecundity of Culex pipiens the vector of Wuchereria bancrofti in Egypt.

The effect of two different doses of ivermectin on the survival and fecundity of Culex pipiens was evaluated. Female mosquitoes (50 in each group), same age and generation, were fed once on blood from ivermectin-treated rabbits. Comparative treatments consisted of two different doses of ivermectin (0.1 mg or 0.4 mg/kg of body weight), and mosquito groups were fed on the rabbits at 3 days and 10 days post-treatment respectively. Mosquitoes were maintained with 10% sucrose solution, at 25 degrees C and 70% relative humidity. Observations were made at 7, 10, 15, 20, 25, and 30 days post-feeding. Mosquitoes fed on rabbits treated with the small dose at three days post-injection showed reduced survival of 94%, 62%, 44%, 8% and 0%, while the 0.4 while the 0.4 mg/kg of body weight (bw) dose reduced survival to 88%, 60%, 6% and 0%. Survival of the control groups were 100%, 90%, 88%, 68% and 52% respectively. Survival of adult females fed at the lower dose, 10 days post injection were reduced to 92%, 90%, 84% 60% and 44%; while the higher dose reduced survival to 86%, 58%, 34% 22% and 0%. Survival of control groups were 96%, 92%, 84%, 78% and 72% respectively. Fecundity resulting from blood meals at 3 and 10 days post injection were 61% and 85% for the lower dose, and 0% and 42% for the higher dose respectively. Hatching of eggs was 82% and 88% for the 0.1 mg/kg bw dose, and no hatching was observed at the higher dose. Larval survival rates resulting from blood meals taken 3 days and 10 days post-injection of the lower dose were 24% and 25% respectively. However, no larvae survived from the high dose groups. Larval survival rates in control groups were 42% and 40%, respectively. These results provide new information about the effect of ivermectin on the vector of lymphatic filariasis, and add a new dimension to the use of ivermectin in filariasis control.

Animals↗

A simple technique for the in vitro cultivation of nocturnally subperiodic Brugia malayi infective larvae.

A simple system for the in vitro cultivation of nocturnally subperiodic Brugia malayi was developed. The manner of cultivation consisted of a 1:1 (v/v) mixture of Iscove's Modified Dulbecco's medium and NCTC-135 medium supplemented with 20% fetal bovine serum by using candle jar incubation at 37 degrees C instead of CO2 incubator. Changing the media: every 2 days, 3 days and changing media on day 7, then every 2 days produced a larval survival rate of 50% (70/140) on day 10, 49% (82/166) on day 6, and 53% (105/200) on day 9. With this technique, up to 50% of the infective stage larvae (L3) survived for up to 10 days and had long life for at least 27 days in all experiments with low larval survival rate in the fourth week. In addition, the culture system promoted molting L3 to fourth stage larvae (L4) after 7 days, as shown by light microscope.

Animals↗

[Sublethal effects of insecticides on fecundity of multicolored Asian ladybird Harmonia axyridis].

The effects of sublethal doses of 6 insecticides (imidacloprid, rotenone, fenvalerate, abamectin, pirimicarb and azadirachtin) on the fecundity of multicolored Asian ladybird Harmonia axyridis were determined respectively. The results showed that in all treatments, the daily average number of eggs per female ladybird was about 40-55. Ladybirds pretreated with imidacloprid by contact and feeding methods, and with rotenone by feeding method produced significantly fewer eggs, compared with control. But the accumulative oviposition of ladybirds pretreated with 6 insecticides at sublethal level was no significant differences, compared with untreated ladybirds. The hatching rates of the eggs laid by ladybirds pretreated with 6 insecticides by two methods were all lower than that of control. The accumulative survivals from egg to pupal stage of all treatments decreased significantly. The developmental duration from egg to pupa of those treated with imidacloprid by two methods and with fenvalerate by contact method prolonged significantly, while that of those treated with rotenone by two methods and with fenvalerate, abamectin, pirimicarb, and azadirachtin by feeding method shortened obviously, but that of those treated with rotenone and abamectin by contact method had no differences with control. After the eggs were immersed with sublethal concentrations of imidacloprid, rotenone, fenvalerate and abamectin, the successful hatching rates were all lower than that of control, and the development periods prolonged clearly. The 1st instar larval survival rates of those treated with fenvalerate decreased significantly, and the survivals from 2nd instar larval to pupal stage also reduced to some extent. The total developmental duration of those treated with rotenone and fenvalerate prolonged significantly. These findings suggested that the effects of insecticides at low doses on the fecundity of ladybird were very complicated. The class, action mode, toxicity and exposure route of insecticides could affect the results of experiments.

Animals↗

Ostreid herpesvirus 1 (OsHV-1) detection among three successive generations of Pacific oysters (Crassostrea gigas).

Ostreid Herpesvirus 1 (OsHV-1) was likely detected in Pacific oysters, Crassostrea gigas, at different stages of development. Viral infections were associated with high mortality rates in the spat and larvae. Furthermore, the persistance of OsHV-1 in asymptomatic adults was demonstrated by detection of viral DNA and proteins. In the present study, three successive generations of C. gigas (G0 and G1 parental oysters, G1 and G2 larvae) were screened for OsHV-1 by PCR. Viral DNA was detected in 2-day-old larvae, indicating that infection may take place at very early stages. Although results strengthen the hypothesis of a vertical transmission, it was not possible to predict the issue of a particular type of cross. Indeed, the detection of viral DNA in parental oysters did not systematically correspond to a productive infection or result in a successful transmission to the progeny. However, the infective status of the parents appeared to have an influence on both the infection and the survival rates of the progeny. Crosses involving an OsHV-1 infected male and a non-infected female resulted in hatching and larval survival rates statistically lower than those observed in the other types of cross. These results suggest that OsHV-1-infected females may transmit to their offspring some kind of protection or resistance against viral infection.

Animals↗

Effect of nitrogen on resistance of sweet potato to sweetpotato weevil (Coleoptera: Curculionidae) and on storage root chemistry.

The effects of nitrogen fertilizer on sweet potato, Ipomoea batatas (L.) Poir., resistance to the sweetpotato weevil, Cylas formicarius elegantulus (Summers), was studied. Adult weevil feeding and oviposition preference, larval survival, and pupal weight were used as measures of sweet potato resistance. Sweet potato resin glycosides and caffeic acid concentrations in the periderm tissue of storage roots also were measured. Sweet potato genotypes (Beauregard, Excel, W-244, W-250, and Sumor) with varying levels of resistance to sweetpotato weevil were grown in the field under three nitrogen regimes (0, 45, and 135 kg N/ha). Harvested storage roots were evaluated in the laboratory for feeding and oviposition activity of sweetpotato weevil female adults under no-choice and choice test conditions. Larval survival rate and pupal weight were determined by rearing the insects individually on storage root sections. Nitrogen level had a significant effect on the number of eggs deposited, but not on the number of feeding punctures. Sweetpotato weevils laid fewer eggs on plants with the highest level of nitrogen. Nitrogen levels did not significantly affect larval survival and pupal weight. Genotype had a significant effect on feeding, oviposition, and larval survival. Beauregard had higher levels of feeding, oviposition, and larval survival compared with the other genotypes. No interaction effects between nitrogen and genotype were significant. Resin glycosides and caffeic acid concentrations were significantly different among genotypes and between years. Nitrogen levels significantly affected the concentrations of caffeic acid in 1997.

Animals↗

Pea lectin expressed transgenically in oilseed rape reduces growth rate of pollen beetle larvae.

In several studies plant lectins have shown promise as transgenic resistance factors against various insect pests. We have here shown that pea seed lectin is a potential candidate for use against pollen beetle, a serious pest of Brassica oilseeds. In feeding assays where pollen beetle larvae were fed oilseed rape anthers soaked in a 1% solution of pea lectin there was a reduction in survival of 84% compared to larvae on control treatment and the weight of surviving larvae was reduced by 79%. When a 10% solution of pea lectin was used all larvae were dead after 4 days of testing. To further evaluate the potential use of pea lectin, transgenic plants of oilseed rape (Brassica napus cv. Westar) were produced in which the pea lectin gene under control of the pollen-specific promoter Sta44-4 was introduced. In 11 out of 20 tested plants of the T0-generation there was a significant reduction in larval weight, which ranged up to 46% compared to the control. A small but significant reduction in larval survival rate was also observed. In the T2-generation significant weight reductions, with a maximum of 32%, were obtained in 10 out of 33 comparisons between transgenic plants and their controls. Pea lectin concentrations in anthers of transgenic T2-plants ranged up to 1.5% of total soluble protein. There was a negative correlation between lectin concentration and larval growth. Plants from test groups with significant differences in larval weights had a significantly higher mean pea lectin concentration, 0.64% compared to 0.15% for plants from test groups without effect on larval weight. These results support the conclusion that pea lectin is a promising resistance factor for use in Brassica oilseeds against pollen beetles.

Animals↗

Interactive effects of diet and temperature on the scope for growth of the scallop Argopecten purpuratus during reproductive conditioning.

Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile were conditioned at two temperatures (16 and 20 degrees C) and three different diets (mixture of pure microalgae, microalgae supplemented with lipids and microalgae supplemented with carbohydrates). The food, equivalent to 3% daily of the animal dry weight was supplied continuously by a peristaltic pump. The rates of different physiological processes were measured on 18 scallops (three replicates per temperature/diet combination) during the third week of conditioning. Nine scallops came from the group conditioned at 16 degrees C and other nine from the group conditioned at 20 degrees C (each scallop from a different tank). As in the conditioning experiment, physiological measurements were made in controlled temperature rooms using the same experimental diets. Argopecten purpuratus did not show significant differences in clearance rate between 16 and 20 degrees C when fed with pure microalgae and microalgae+lipids, showing a well known capability of bivalves to acclimate their physiological rates within a certain range of temperature. On the other hand a clear effect of the composition of the diet on the clearance rate was observed. When microalgae were supplemented with a lipid emulsion, clearance rate was highly stimulated, showing values significantly higher in comparison with either pure microalgae, or a mixed diet of microalgae+carbohydrate. These highest feeding rates can be related with the presence of essential fatty acids in the diet, which are considered as very important compounds during the gametogenesis of invertebrates as well as during the development of ova into normal larvae. Thus A. purpuratus can actively regulate clearance rate and does not simply switch between feeding and non-feeding states. The data also suggest the presence of chemical receptors at the level of the gills and/or labial palps, which seem able to detect specific nutritive compounds present in the diet. Absorption efficiency was independent of temperature and was higher with pure microalgae and with microalgae+lipids. The lowest efficiencies were recorded with the diet supplemented with carbohydrates. The energy expended in oxygen uptake and ammonia excretion was very similar in the different experimental diets and temperatures. Scope for growth in A. purpuratus appears mainly affected by the diet and not by temperature. While the lower SFG seems to be associated with diets composed of pure microalgae and microalgae+carbohydrates, the highest values being found for a diet rich in lipids. The data were in agreement with the reproductive conditioning of A. purpuratus, where the highest percentage of ripe scallops occurred in individuals fed with a diet of microalgae supplemented with lipids at both temperatures. Similarly the highest larval survival rate was obtained from gametes released by scallops conditioned with the diet containing lipids.

Journal Article↗

Female mate choice in a subsocial beetle: male phenotype correlates with helping potential and offspring survival.

In Parastizopus armaticeps (Tenebrionidae), a nocturnal desert beetle, the males excavate breeding burrows and maintain their moisture level while the females provision the larvae with detritus collected on the surface. The beetles court in small groups on the surface at night after rain. Male size distribution in these groups corresponded to that in the population but more large and fewer small females were present than expected and more large beetles of both sexes bred. Offspring number correlated positively with burrow depth and body length for males but not for females. Since large males dig deeper burrows, which results in higher larval survival rate, females should prefer them. In choice experiments, females selected larger males. Behavioural analyses showed that choice was not dependent on differences in male courtship activity or intermale dominance. When the mass of the smaller male was increased experimentally by a dorsally attached weight, the smaller male was preferred, females estimating male size difference by mass. Partner choice is therefore epigamic for a male phenotypic character which correlates with both parenting ability and greater reproductive success for females. Copyright 1998 The Association for the Study of Animal Behaviour.

Journal Article↗

Positive and negative effects of leaf shelters on herbivorous insects: linking multiple herbivore species on a willow.

We experimentally examined the effects on other herbivorous insects of leaf shelters constructed by lepidopteran larvae on a willow, Salix miyabeana. Several insect species occupied the vacant leaf shelters. Our experiment using artificial leaf shelters showed that the number of aphids increased with the number of artificial leaf shelters on a shoot, as did the numbers of three ant species ( Camponotus japonicus, Lasius hayashi, and Myrmica jessensis) that entered leaf shelters to collect aphid honeydew. To determine the ant-mediated effect of leaf shelters on herbivorous insects that do not use leaf shelters, we transferred newly hatched larvae of a common leaf beetle, Plagiodera versicolora, to the leaves of shoots with and without artificial leaf shelters. One day after the transfer, larval survival rate was significantly lower on shoots with shelters than on those without shelters, and shoots with shelters had significantly more ants than did shoots without shelters. Our field experiments demonstrated clearly that shelter-making lepidopteran larvae increased the abundance of both aphids and ants and decreased the survival rate of leaf beetle larvae, probably because the larvae were removed by ants that were attracted to the leaf shelters by the aphid colonies.

Animals↗

Effects of seven antibiotics on the growth and development of Phaenicia sericata (Diptera: Calliphoridae) larvae.

Maggot therapy is used for patients with severe tissue destruction, who often receive antibiotics concurrently. Therefore, we studied the effects on maggots of ampicillin, cefazolin, ceftizoxime, clindamycin, gentamicin, mezlocillin, and vancomycin in concentrations of 1, 10, 100, and 1,000 times the average minimum bactericidal or bacteriostatic concentration against highly susceptible organisms. There was a reduction in larval survival in media with gentamicin at concentrations of 1,000 times the average pharmacologic level, or 4,000 micrograms/ml (2.7% survival), versus lower concentrations (80-88% survival). Maturation of the surviving pupae to adults also was decreased at this concentration. Media with cefazolin concentrations of 100 times the average bacteristatic level, or 800 micrograms/ml, also led to a significant decrease in larval survival (70% versus 80-88%). There were no differences in larval survival, rate of maturation, or pupal weights for organisms reared on media containing ampicillin, ceftizoxime, clindamycin, mezlocillin, or vancomycin. P. sericata matured normally--and thus can be used therapeutically--when exposed to standard pharmacologic levels of the 7 antibiotics tested. Furthermore, the methods employed herein may be helpful to forensic entomologists attempting to develop models for drug ingestion by maggots.

Animals↗

Development of Epiphyas postvittana (Lepidoptera: Tortricidae) on leaves and fruit of orange trees.

Development of Epipyas postvittna (Walker) (Lepidoptera: Tortricidae), on leaves and fruit of 'Valencia', 'Washington navel', and 'Navelina' orange trees was studied under constant and fluctuating temperatures. E. postvittna was able to complete its life cycle feeding exclusively on leaves or fruit of orange trees. However, larval survival rate was very low (< 20%) on orange tissues compared with that on noncitrus hosts. Among the four types of orange tissues, young orange leaves and fruit afforded larvae higher survival rates than mature orange leaves and fruit. Fruit (young or mature) produced heavier pupae than leaves (young or mature). Larvae developed more slowly on mature orange fruit than on other orange materials and more slowly on orange leaves than on leaves of most noncitrus hosts. Degree-day accumulations based on the fastest developmental rates obtained in this study suggested that E. postvittna is capable of completing 4.4-4.7 generations per year in orange orchards in the Riverina region of New South Wales, Australia. Implications of the results in the management of the insect in citrus are discussed.

Animals↗

Electroporation as an effective means of introducing DNA into abalone (Haliotis rufescens) embryos.

Recombinant plasmid containing the Drosophila beta-actin promoter coupled to a beta-galactosidase cassette was linearized and introduced in fertilized eggs of the red abalone (Haliotis rufescens) by electroporation. Fertilized abalone eggs tolerated electroporation well with larval survival rates between 70% and 84% of that for non-electroporated siblings. Dot blot and Southern blot analysis were used to detect if abalone retained the foreign gene at various developmental stages. The inserted construct was retained in 70% to 100% of all abalone sampled with an average of 72% retention in the three- to seven-month-old juveniles. Maximal DNA uptake and retention was observed in abalone electroporated at 30-40 min after fertilization. Southern hybridization analysis suggested that the inserted vector was in head-to-tail concantermers integrated in the abalone genome. This preliminary study demonstrates that electroporation is an efficient means of transferring foreign DNA into abalone embryos.

Actins↗

Mass rearing the weevil Hylobius transversovittatus (Coleoptera: Curculionidae), biological control agent of Lythrum salicaria, on semiartificial diet.

Lythrum salicaria L., purple loosestrife, an invasive Eurasian perennial, is degrading wetlands across temperate North America. Chemical, physical, and mechanical methods failed to provide long-term control. Therefore, four host-specific insect species (two leaf feeders, a root feeder, and a flower feeder) were introduced as biological control agents. To increase the availability of adults of the root feeding weevil Hylobius transversovittatus Goeze for field releases, we developed a semiartificial diet. Suitability of different diet formulations (varying vitamin mixes, salt mixes, antimicrobials, water content, root content) and temperatures for larval development were evaluated. We also monitored how rearing on artificial diet and the number of larvae per container affected larval development time, larval survival, adult weight, and incidences of deformities. Rearing larvae on artificial diet reduced development time from 1-2 yr to 2-3 mo. Larval development was fastest and survival rates highest under constant temperatures of 25 degrees C. Hatch rate and larval survival decreased if eggs were surface sterilized. Using FABCO antimicrobials could not prevent fungal contamination; use of methyl paraben and sorbic acid was successful in suppressing fungal and bacterial growth throughout larval development time to <10%. The moisture content of the diet did not significantly affect larval survival, development, or adult weight. Decreasing the proportion of purple loosestrife roots in the diet reduced adult weight and the proportion of larvae completing development, and increased development time; no larvae were able to complete development in root-free diet. With an increase in the number of larvae per cup, survival rates were reduced. The experiments revealed a female biased sex ratio: females consistently developed faster and were heavier than males. Incidence of adult deformities was consistently below 5%. Increased availability of adults for field release as a result of mass production using the semiartificial diet will accelerate the biocontrol program targeting purple loosestrife. We are able to produce several hundred weevils per week and have adapted the diet to rear other root-feeding weevils.

Animals↗

Laboratory rearing techniques and adult life table parameters for Anopheles sergentii from Egypt.

Techniques are presented for maintaining colonies of Anopheles sergentii, an important malaria vector in Egypt. Larval development time and survival rates were determined for 3 rearing solutions and 4 temperatures. Under optimal conditions larval survival rates averaged 85%. Mean life expectancy at emergence for mated An. sergentii was 23.3 days under insectary conditions of 27 +/- 2 degrees C, 70-80% R.H. The net reproductive rate, mean generation time and instantaneous rate of increase were respectively, 45.8 females per female per generation, 29.7 days and 0.127. In the context of vector potential for malaria transmission, An. sergentii has a daily survivorship rate of 0.95.

Actuarial Analysis↗

Potential of entomopathogenic nematodes for biological control of Acalymma vittatum (Coleoptera: Chrysomelidae) in cucumbers grown in conventional and organic soil management systems.

Acalymma vittatum (F.) is the primary insect pest of fresh-market cucumber and melon crops in much of the eastern United States because of their herbivory and interactions with several diseases, most notably bacterial wilt. A study was conducted to determine how soil management affects viability and infectivity of an entomopathogenic nematode that may be used for the control of A. vittatum. Dose-mortality curves under laboratory conditions suggested several Steinernema spp. as potential biocontrol agents. Field injections combined with soil bioassays showed that Steinernema riobravis Cabanillas, Poinar & Raulston (Rhabditus: Steinernematidae) longevity exceeded A. vittatum immature development time in both conventional and organic soil management systems. Mean root length densities of cucumbers increased in both soil management systems with the inclusion of nematodes. Soil management alone also influenced A. vittatum larval survivorship, with higher survival rates in the organic compared with the conventional soil management system. A 50% reduction in A. vittatum larval survival rates in both soil management systems, as determined by adult A. vittatum emergence, demonstrated the potential of incorporation of entomopathogenic nematodes for integrated pest management of diabroticites in commercial cucumber production.

Agriculture↗

Evolution of accelerated senescence in laboratory populations of Drosophila.

Ecological theories of life history evolution predict that natural selection should favor semelparous life histories in environments where juvenile survival is high relative to adult survival and rates of population growth are high. That is, organisms should complete their entire reproductive effort in a short period of time following maturation. Direct empirical verification of this idea has been lacking. Six independent populations of Drosophila melanogaster were maintained in two different environments, called r and K, for more than 120 generations. In the r environment population size was small, larval survival and rates of population growth were high, and reproduction was limited to a few days after eclosion. In the K environment population size was large and larval survival low, but adults were allowed to reproduce indefinitely. The fecundity of females of different sizes from each environment was measured daily for 4 weeks. No differences in fecundity were seen during the first week of adult life for females from the two environments. By the fourth week, however, the fecundity of large females from the r environment was 47-83% less than that of females from the K environment. The accelerated senescence exhibited by females from the r environment appears to be due to the accumulation of deleterious alleles whose effects are expressed late in life, which is consistent with the mutation accumulation hypothesis for the evolution of senescence.

Aging↗