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At least 19 recordsLinked to original sources

Particle size distribution of Serratia marcescens aerosols created during common laboratory procedures and simulated laboratory accidents.

Andersen air samplers were used to determine the particle size distribution of Serratia marcescens aerosols created during several common laboratory procedures and simulated laboratory accidents. Over 1,600 viable particles per cubic foot of air sampled were aerosolized during blending operations. More than 98% of these particles were less than 5 mu in size. In contrast, 80% of the viable particles aerosolized by handling lyophilized cultures were larger than 5 mu. Harvesting infected eggs, sonic treatment, centrifugation, mixing cultures, and dropping infectious material produced aerosols composed primarily of particles in the 1.0- to 7.5-mu size range.

Accidents

The ceramic reverse three-quarter crown for anterior teeth: laboratory procedures.

The laboratory procedures for making a Hi-ceram porcelain anterior reserve three-quarter crown are described. A core of high-fusing aluminum oxide is used as a coping on which tooth contours are restored with a compatible porcelain. Standard dental laboratory equipment is used and the technique can be quickly learned by any experienced ceramics technician.

Aluminum

Forecasting hospital laboratory procedures.

Improved forecasts of hospital laboratory procedures can provide the basis for better resource planning and enhanced operating efficiency. The research reported here-in describes how multiple regression models can be both a source of insight into causal relationships and a tool for achieving accurate monthly forecasts. Past research in this area may have overstated the statistical significance of findings because of a failure to address the potential effect of serial correlation. The present study uses the Cochrane-Orcutt regression procedure, rather than OLS, to overcome this problem. A model using inpatient admissions, acuity days, length of stay, discharge days and seasonal dummy variables is shown to account for 87% of the variation in the number of billable laboratory procedures. A simpler multiple regression model and a Winters' exponential smoothing model were found to provide excellent forecasts for laboratory procedures. In a one year out of sample evaluation, the annual percent forecast error was 0.7% for the regression model. This compares favorably to a percentage forecast error of 11.6% using subjective forecasting methods.

Forecasting

Laboratory procedures used in the diagnosis of systemic lupus erythematosus: a review.

The literature concerning the laboratory procedures presently available to aid in the diagnosis of systemic lupus erythematosus (SLE) was reviewed to determine which of these techniques could be most valuable in the detection and management of SLE patients. The LE cell test, once the laboratory basis for SLE diagnosis, was concluded to be insensitive, non-specific, and did not correspond to clinical activity of the patient. A second procedure, antinuclear-antibody detection, although very sensitive, was not specific for SLE; therefore, its value is limited for use as a screening technique to rule out SLE. The Farr anti-DNA precipitate immunoassay, used for the measurement of antibodies to DNA, was sensitive and specific, and also correlated well with the clinical condition of the patient. Therefore, the Farr binding assay is recommended as the laboratory procedure of choice since it is useful in monitoring disease activity and may contribute to earlier diagnosis and more precise management of SLE patients.

Antibodies, Antinuclear

Medical student training in the performance of diagnostic laboratory procedures.

A study was conducted to determine what diagnostic laboratory procedures medical students recalled being taught during pre-clerkship training, how well they felt they could perform these procedures on entrance to and exit from their clerkship year, and the estimate of frequency of personal performance during the clerkship year. Surveys were mailed to 223 graduating senior students of a medical school. They were asked to supply data regarding 15 pre-selected procedures. In only seven cases did a majority of students recall being taught a procedure. Higher percentages of students who trained at health science centres and a Veterans Administration hospital recalled being taught procedures compared to students who trained at community hospitals. In general, students who performed their pre-clerkship training at the health science centres rated their ability to perform procedures without assistance on entrance to the clerkship year higher than the other two groups. Students who performed at least one half of their clerkships at a health science centre rated their ability to perform procedures without assistance at the termination of their clerkship year higher than those who performed a majority of clerkships at community hospitals. The former group also reported a higher frequency of performance of the procedures than the community hospital group. Most of the procedures for all groups, however, were performed at a rate of less than one/month.

Chicago

Laboratory procedures for the maintenance of a removable partial overdenture.

Laboratory procedures in support of maintenance therapy for a typical patient with a removable partial overdenture were described. These procedures consisted of the fabrication of a gold metal coping and the reline of the prosthesis with autopolymerizing acrylic resin. In designing the coping contours, the technician must consider the path of insertion of the prosthesis and the space requirement for the overlying resin tooth. Reproduction of the dowel preparation is facilitated by using a plastic dowel as part of the coping wax pattern. The removable partial overdenture was relined with autopolymerizing acrylic resin in a reline jig. Tooth-colored acrylic resin was used in the concavity formed by the overdenture abutment to enhance esthetics. The requirement of a coordinated effort between the dentist and the dental laboratory technician is critical to the successful treatment of the removable partial overdenture patient.

Acrylic Resins

Animal procedure laboratory surveys: use of the animal laboratory to improve physician confidence and ability.

Animal laboratories have been used for many years to teach procedures. Our department has a weekly swine laboratory devoted to training residents, interns and students in resuscitative procedures. Physicians who participated in our swine procedure laboratory over the past three years were queried as to their prelaboratory and postlaboratory comfort levels with six different resuscitative procedures, and 57 (76%) physicians responded. Statistical analysis of the data showed significant improvement in comfort levels for all six procedures. Every responder felt the swine laboratory helped or will help them perform the procedures on humans. We also surveyed all U.S. emergency medicine residency program directors from established programs regarding the status of their animal procedure laboratories, and 67 of the 68 (98%) directors responded. Of these responders, 62% offer an animal procedure laboratory. Overall, 97% of the residency directors rated the laboratory successful, and 97% of the residents rated the laboratory successful. Therefore, we conclude that an ongoing emergency medicine animal procedure laboratory is a valuable tool for improving physician-in-training ability and confidence.

Animals

Laboratory procedures for the fabrication of maxillary occlusal bite plane splint.

Clear communication between the dentist and laboratory technician is important for the production of quality work and the saving of valuable chair time at delivery. This article discusses the laboratory procedures used in the fabrication of occlusal bite plane splint. Traditional heat curing, microwave heat processing, and visible light curing techniques are discussed.

Acrylic Resins

A simplified laboratory procedure to select an appropriate antibiotic for treatment of refractory periodontitis.

THE FEASIBILITY OF USING A simplified laboratory procedure to select an appropriate antibiotic for treatment of refractory periodontitis was investigated. Six different antibiotics were added to commercially available CDC-agar plates. Plaque samples from periodontal pockets were inoculated onto the plates. The percentage kill was determined by comparing colony counts on the antibiotic-containing medium with those on the control plates. The three most effective antibiotics were ranked by visual inspection as well as by colony count. It was determined that visual inspection may be used to determine an effective antibiotic.

Ampicillin

Physician utilization of laboratory procedures to monitor outpatients with congestive heart failure.

Little is known about how different types of physicians use laboratory procedures in the management of outpatients with congestive heart failure. We therefore analyzed data from a national survey of randomly selected general practitioners, internists, and cardiologists to assess their management of outpatients with New York Heart Association class II congestive heart failure. Most of the 2704 respondents (90%) scheduled office visits between two and four months apart. Body weight, serum electrolytes, and chest roentgenograms were followed regularly by 98% or more of respondents, at median intervals of one to two months, three to five months, and 12 to 17 months, respectively. Serum digoxin levels in patients taking digoxin were followed by 90% of respondents at a median interval of 12 months. Echocardiography, radionuclide ventriculography, and exercise testing were used by fewer respondents (81%, 61%, and 61%, respectively), each at a median interval of 18 months or longer. Cardiologists were significantly more likely to follow patients using either echocardiography, radionuclide ventriculography, or exercise testing. The estimated yearly cost of following a class II congestive heart failure outpatient varied nearly fourfold from the lowest quartile of physicians ($303) to the highest ($1167). Cardiologists were disproportionately represented among the high-cost users. In addition, physicians who were younger or who practiced in an urban setting were significantly more likely to be high-cost users. Thus, simple laboratory tests were used most frequently to follow patients with heart failure, but differences in use of more expensive tests led to large differences in cost. Test use for similar patients is affected by characteristics of both the physician and practice setting.

Ambulatory Care

Development of a procedures laboratory evaluation tool.

Historically, it has been a relatively simple matter to assess students' classroom performance via internally developed evaluations. However, procedures laboratory proficiency of a student has been grossly neglected and has often fallen prey to the subjectivity of the evaluator. Accurate evaluation of students in the laboratory setting requires use of an effective evaluation instrument. The evaluation format described in this article was developed to effectively evaluate and critique the student's proficiency in radiographic positioning.

Alabama

Clinical praxis and laboratory procedures in subrenal capsule assay (SRCA).

Subrenal capsule assay (SRCA) is a promising method in cancer research and in the selection of individual chemotherapy for cancer patients. The laboratory procedures of SRCA are as follows: on day zero 1 X 1 X 1 mm pieces of fresh human tumour are carefully prepared. The pieces are transferred by a trocar under the outer capsule of normal immunocompetent mice, one piece to each. The mice (about 30-40/test) are divided randomly into groups of at least five. The size of each piece (initial size) is measured in situ by a stereomicroscope fitted with an ocular micrometer. On days 1-5 cytostatics are administered to the animals. On day 6 the animals are killed and the final tumour size is measured. The difference between the final and initial tumour size describes the response of the tumour to the treatments. The critical point of the assay is the selection of the tumour fragment which has to be representative, as homogeneous as possible and contain living tumour cells. When the sample is in pieces of about 3 X 3 mm (4-5 tumour fragments are sufficient for the assay) in the culture medium, it may be stored in an ice bath or at room temperature for one day. During this time, though as rapidly as possible, the sample has to be delivered to a research laboratory where the SRCA is carried out.

Animals

[Displacement and distortion of non-precious ceramometal fixed bridges during the laboratory procedure. Non-precious metal].

The purpose of this study is to measure the three-dimentional displacement and distortion in the Non-precious (Ni-Cr) ceramometal bridges during laboratory procedure. At the time of pre-soldering stage, the author examined the phosphatic investment (low and high thermal expantion: ph, ps) to addition of gypsum investment which was usually to used to fix the position for soldering. The following results were obtained. 1. The displacement of the framework at the pre-soldering stage, in case of using phosphatic investment mixed by water (ph), it was small on the horizontal and vertical plane. And in the other case of mixing by 100% colloidal silica (ps), a kind of displacement was seen where the curve of the bridge opened as result of the position of two soldering bodies getting out of order from each other. So that phosphatic bonded investment, it can be used to because thermal expantion can be controlled, the soldering block. 2. Throughout the surface grinding of framework to grazing, displacement was minimal, at the time of opaque porcerain firing, the curve of the bridge closed but it had no effect on clinical adaptation. 3. In the case of assembling multi-unit non-precious ceramometal bridge mainly the displacement caused at pre-soldering stage, so it is important to check the adaptation after soldering.

Chromium Alloys

Stereo laser-welded titanium implant frameworks: clinical and laboratory procedures with a summary of 1-year clinical trials.

A new technology for implant framework fabrication (Procera) has been introduced to North America after initial clinical trials in Sweden. This technique is unique because it eliminates the conventional approach to framework fabrication with the lost wax casting technique. Clinical and laboratory procedures associated with the Procera technique are described in this article, as are the most recent developments in this rapidly emerging technology. A report of 1-year results of the first 10 patients treated with the Procera technique at the University of Washington is described. Treatment outcome has been favorable, with no clinical evidence of prosthodonic or soft-tissue complications noted. All 50 of the implants placed in the 10 patients remain integrated after 1 year of service. The Procera technology offers an alternative to current conventional framework fabrication techniques. Continued follow-up of this patient series is pursued to explore the treatment outcome for a longer interval of time.

Aged