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At least 19 recordsLinked to original sources
PubMed2005

Larvicidal, miracidiacidal and cercaricidal activities of the Egyptian plant Iris pseudacorus.

The larvicidal properties of Iris pseudacorus leaves ethanol extract against second-instar larvae of two mosquito species, Culex pipiens and Aedes caspius (Diptera: Culicidae) were studied. It was observed that the larvicidal effect of this extract was dose-dependent. The LC50 values of I. pseudacorus against C. pipiens and A. caspius were 10.36 and 16.43 mg/l within 24 hr, and 7.36 and 10.1 mg/l within 48 hr, respectively. The miracidiacidal and cercaricidal properties of I. pseudacorus extract were directly tested against Schistosoma mansoni miracidia and cercariae and a time-concentration relationship was observed. The concentrations needed to kill all miracidia (LC100) within 5 min., 30 min. and an hr of exposure were 2.7, 1.6 and 0.9 mg/l respectively. The concentrations needed to kill all cercariae (LC100) within 5 min. 30 min. and an hr of exposure were 1.5, 1.0 and 0.6 mg/l respectively.

Aedes↗
PubMed2003

[RbcL sequence analysis of Belamcanda chinensis and related medicinal plants of Iris].

AIM: To identify "Shegan" [Belamcanda chinensis (L.) DC.] and relative medicinal plants of Iris including Iris tectorum Maxim., I. dichotoma Pall., I. germanica L. and I. japonica Thunb. by ribulose 1,5-bisphosphate carboxylase Large Gene (rbcL) sequence analysis. METHODS: General DNA was isolated from the fresh leaves of Belamcanda chinensis and 4 Iris spp. by CTAB. A pair of primers was designed to amplify the rbcL gene and PCR Preps DNA kit was used to purify the PCR products. The rbcL sequences were determined by ABI (Applied Biosystems Inco.) Prism 310 Genetic Analyzer. RESULTS: A fragment of about 750 bp of rbcL gene from Belamcanda chinensis and 4 Iris spp. were amplified and sequenced. The rbcL sequences of Iris tectorum, I. dichotoma Pall. and I. japonica were reported for the first time. The rbcL sequences of 5 species of Iridaceae were aligned and analyzed using Clustal (Version 8.0) and MEGA (Version 2.0.) programs. The nucleotide number of difference is from 1.000 to 20.000. The tranversions is from 0.000 to 9.000 and the transitions is from 0.000 to 14.000. Phylogenetic tree based on rbcL partial sequence data indicated that the eleven samples of 5 species clustered separately. CONCLUSION: The sequence variation of rbcL can be used to identify Belamcanda chinensis and 4 species of relative medicinal plants of Iris. The molecular phylogenetic tree accords with the classical taxonomy.

Base Sequence↗
PubMed2001

Intimately linked or hardly speaking? The relationship between genotype and environmental gradients in a Louisiana Iris hybrid population.

Several models of hybrid zone evolution predict the same spatial patterns of genotypic distribution whether or not structuring is due to environment-dependent or -independent selection. In this study, we tested for evidence of environment-dependent selection in an Iris fulva x Iris brevicaulis hybrid population by examining the distribution of genotypes in relation to environmental gradients. We selected 201 Louisiana Iris plants from within a known hybrid population (80 m x 80 m) and placed them in four different genotypic classes (I. fulva, I. fulva-like hybrid, I. brevicaulis-like hybrid and I. brevicaulis) based on seven species-specific random amplified polymorphic DNA (RAPD) markers and two chloroplast DNA haplotypes. Environmental variables were then measured. These variables included percentage cover by tree canopy, elevation from the high water mark, soil pH and percentage soil organic matter. Each variable was sampled for all 201 plants. Canonical discriminant analysis (CDA) was used to infer the environmental factors most strongly associated with the different genotypic groups. Slight differences in elevation (-0.5 m to +0.4 m) were important for distinguishing habitat distributions described by CDA, even though there were no statistical differences between mean elevations alone. I. brevicaulis occurred in a broad range of habitats, while I. fulva had a narrower distribution. Of all the possible combinations, I. fulva-like hybrids and I. brevicaulis-like hybrids occurred in the most distinct habitat types relative to one another. Each hybrid class was not significantly different from its closest parent with regard to habitat occupied, but was statistically unique from its more distant parental species. Within the hybrid genotypes, most, but not all, RAPD loci were individually correlated with environmental variables. This study suggests that, at a very fine spatial scale, environment-dependent selection contributed to the genetic structuring of this hybrid zone.

Altitude↗
PubMed2002

[Variability of life cycle traits in Iris pumila juvenile plants: plant shoots and roots dynamics].

In this study I analyzed dynamic of ramet number on 18 half-sib and 63 full-sib families of rhizomatous perennial herb Iris pumila originating from two Deliblato Sand populations and grown in two nutrient levels (full strength and 1/10 of full strength Hoagland solution). Contrary to most studies dealing with response to different nutrient availability, I failed to detect significant mean nutrient level effect on dynamic of module emergence. Such absence of consistent response to tenfold variation in solution strength indicate that I. pumila have low saturation rates of nutrient uptake and presumably experienced both utilized nutrition levels as rich ones. Genetic variability for plastic response was lacking either. On the other hand, presence of between-population differentiation for the trait itself indicates that I. pumila harbors considerable amounts of within-species genetic variability for ramet dynamics.

Crosses, Genetic↗
PubMed2005

Variation in survival of Iris pumila L. juvenile plants grown in two nutrient regimes.

I analyzed variability of early life survival on 881 Iris pumila juvenile plants in their prereproductive period. Seedlings were produced by applying half-sib mating scheme on clones originating from two Deliblato Sand populations that were accommodated to common garden conditions. Nineteen clones served as pollen donors (sires), and the other 90 clones served as pollen recipients (dames). Seedlings were grown in two nutrient levels (full strength and 1/10 of full-strength Hoagland solution). While I failed to detect significant mean nutrient level effect as well as significant between population differentiation for prereproductive survival, a statistically significant additive genetic variability for this ultimate prereproductive measure of fitness was detected.

Crosses, Genetic↗
PubMed2004

The type-1 and type-2 ribosome-inactivating proteins from Iris confer transgenic tobacco plants local but not systemic protection against viruses.

The antiviral activity of the type-2 ribosome-inactivating protein (RIP) IRAb from Iris was analyzed by expressing IRAb in tobacco (Nicotiana tabacum L. cv. Samsun NN) plants and challenging the transgenic plants with tobacco mosaic virus (TMV). Although constitutive expression of IRAb resulted in an aberrant phenotype, the plants were fertile. Transgenic tobacco lines expressing IRAb showed a dose-dependent enhanced resistance against TMV infection but the level of protection was markedly lower than in plants expressing IRIP, the type-1 RIP from Iris that closely resembles the A-chain of IRAb. To verify whether IRIP or IRAb can also confer systemic protection against viruses, transgenic RIP-expressing scions were grafted onto control rootstocks and leaves of the rootstocks challenged with tobacco etch virus (TEV). In spite of the strong local antiviral effect of IRIP and IRAb the RIPs could not provide systemic protection against TEV. Hence our results demonstrate that expression of the type-1 and type-2 RIPs from Iris confers tobacco plants local protection against two unrelated viruses. The antiviral activity of both RIPs was not accompanied by an induction of pathogenesis-related proteins. It is suggested that the observed antiviral activity of both Iris RIPs relies on their RNA N-glycohydrolase activity towards TMV RNA and plant rRNA.

Disease Susceptibility↗
PubMed2006

Detecting adaptive trait introgression between Iris fulva and I. brevicaulis in highly selective field conditions.

The idea that natural hybridization has served as an important force in evolutionary and adaptive diversification has gained considerable momentum in recent years. By combining genome analyses with a highly selective field experiment, we provide evidence for adaptive trait introgression between two naturally hybridizing Louisiana Iris species, flood-tolerant Iris fulva and dry-adapted I. brevicaulis. We planted reciprocal backcross (BC1) hybrids along with pure-species plants into natural settings that, due to a flooding event, favored I. fulva. As expected, I. fulva plants survived at much higher rates than I. brevicaulis plants. Backcross hybrids toward I. fulva (BCIF) also survived at significantly higher rates than the reciprocal backcross toward I. brevicaulis (BCIB). Survivorship of BCIB hybrids was strongly influenced by the presence of a number of introgressed I. fulva alleles located throughout the genome, while survivorship in the reciprocal BCIF hybrids was heavily influenced by two epistatically acting QTL of opposite effects. These results demonstrate the potential for adaptive trait introgression between these two species and may help to explain patterns of genetic variation observed in naturally occurring hybrid zones.

Chimera↗
PubMed2005

Nucleocytoplasmic trafficking in plant cells.

In a eukaryotic cell, the nuclear envelope (NE) separates genetic information from the environment of biosynthesis and metabolism. Transfer of macromolecules across the NE involves the nuclear pores--large multisubunit protein complexes--and machinery that facilitates rapid, directional, and selective transport. While core elements of the transport process are conserved between kingdoms, different solutions to similar problems have also evolved. Although the structure and composition of the yeast and mammalian nuclear pore have been unraveled recently, the plant nuclear pore remains largely enigmatic. Like any other process, nucleocytoplasmic transport can be regulated. Several examples from plants are discussed that promise insights into the regulation of signaling pathways. While controlling the partitioning of cellular components, the nuclear envelope also presents an obstacle to viruses and transforming agents that need access to the genome, and different mechanisms have evolved to overcome this obstacle. Finally, the recent recognition of the importance of small RNAs for gene regulation emphasizes the need to understand small RNA nuclear export and the levels of its regulation. This review attempts to wed our molecular-mechanistic understanding of nucleocytoplasmic trafficking drawn from all model systems with the intriguing examples of regulated nucleocytoplasmic partitioning in plants.

Active Transport, Cell Nucleus↗
PubMed2003

Anti-inflammatory isoflavonoids from the rhizomes of Iris germanica.

The anti-inflammatory activity of nine isoflavonoids 5,7-dihydroxy-3-(3'-hydroxy-4',5'dimethoxy)-8-methoxy-4H-1-benzopyran-4-one 1, 5,7-dihydroxy-3-(3'-hydroxyl-4', 5'-dimethoxy)-6-methoxy-4H-1-benzopyran-4-one 2, 5, 7-dihydroxy-3-(4'-hydroxy)-6-methoxy-4H-1-benzopyrane-4-one 3, 5-hydroxy-3-(4'-hydroxy)-6,7-methylenedioxy-4H-1-benzopyran-4-one 4, 5-hydroxy-3-(4'-methoxy)-6,7-methylenedioxy-4H-1-benzopyran-4-one 5, 5-methoxy-3-(4'-hydroxy)-6,7-methyenedioxy-4H-1-benzopyran-4-one 6, 5,7-dihydroxy-3-(3'-hydroxy-4'-methoxy)-6-methoxy-4H-1-benzopyran-4-one 7, 5,7-dihydroxy-3-(3'-methoxy-4'-hydroxy)-6-methoxy-4H-1-benzopyran-4-one 8, and isopeonol 9 determined by a spectrophotometric assay using the activated human neutrophils. These isoflavonoids were isolated from an important folkloric medicinal plant Irsa (Iris germanica L.), a member of the family Iridaceae. Structures of these compounds were identified by spectral comparison with the reported data and active members of this group adds into the growing number of non-steroidal anti-inflammatory agents.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1998

[Karyotype analysis on Iris lactea ssp. chinensis].

In this paper, the chromosome number, karyotype and chromosome volume of Iris lactea ssp. chinensis were reported. The normal diploid was 2n = 32. Karyotype formula was based on Levan's publication in 1964, k (2n) = 32 = 22 m + 8 sm + 2T. According to the method of Kuo SR, the species chromosome based on the relative length was 2n = 32 = 6L + 4 M2 + 18 M1 + 4 S which belong to "2A" type according to the Stebbins' karyotype classification. The AS.K% was 62.5% from Arano's method. The total volume of chromosome groups was 34.95 micron 3.

Base Sequence↗