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Results for “Histological Techniques”

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At least 19 recordsLinked to original sources
PubMed1977

Evaluation of a self-instructional unit for use in educational programs in histologic technique.

A linear programmed instructional booklet was developed and used to evaluate the acceptability of this learning strategy to histologic technique instructors and their students. Comparisons of students' cognitive knowledge of the relevant topics before and after using the booklet indicated that most were achieving the booklet's objectives. The post-test score was not significantly affected either by prior experience with programmed materials or by the way the booklet was used. The verbal attitude responses of the students were encouraging, and most indicated the booklet was an efficient way for them to learn, and it was helpful in clarifying concepts. The verbal attitudes of the instructors were also favorable. The indications were that booklet use shortened subsequent lecture time and that the booklet was most valuable as a supplement to conventional instructional methods.

Achievement↗
PubMed1976

Comparative evaluation of various histological techniques for the rapid diagnosis of brain tumours.

Intraoperative procedures for the rapid histological diagnosis of space occupying intracranial processes are required. These currently include three major techniques: 1. crush preparations; 2. frozen sections with prefixation, and 3. frozen sections without prefixation. We have compared these techniques, using identical tissue material. While frozen sections of samples subjected to rapid fixation produce the best specimens, crush preparations are preferred wherever a well equipped laboratory is not available.

Astrocytoma↗
PubMed1977

The atrioventricular node, His bundle and bundle branches-a new histologic technique.

A technique for examination of the conducting system of the heart is described. A block of tissue embracing the ostium of the coronary sinus, the pars membranacea, the septal leaflet of the tricuspid valve, and appropriate amounts of interatrial and interventricular septum is flattened and fixed in a Kaiserling I solution. Blocks are subsequently cleared in methyl salicylate and trimmed. Sections are cut from the blocks after paraffin embedding beginning from the endocardial surfaces of the right heart chambers. The sections are mounted and stained on 35 mm unperforated leader film and covered with an acrylic preservative. For examination of the conducting system many fewer sections are required than with previous techniques.

Atrioventricular Node↗
PubMed1979

Testicular biopsy in the study of male infertility: its current usefulness, histologic techniques, and prospects for the future.

Testicular biopsy has been widely used for the diagnosis of male infertility for more than three decades. During that time, with advances in cytogenetics, radioimmunoassay, and endocrinology, the role of testicular biopsy has changed. Testicular biopsy is still useful in the diagnosis of azoospermic and oligospermic males without stigmata of gonadotropic insufficiency or Klinefelter's syndrome. A classification and description is presented of pathologic changes in the testis as seen on testicular biopsy by light microscopy. The present rationale for testicular biopsy in infertility, the processing and staining of histologic material, and the role of testicular biopsy in infertility and infertility related situations, including cryptorchidism, malignant disease, and chemotherapy related changes, are discussed. Further understanding of testicular function and disease will depend upon the correlation of histologic and ultramicroscopic changes, immunohistologic localization of hormones, and epidemiologic and endocrinologic data.

Adolescent↗
PubMed1978

[Histological technique for intramural ganglia in thick tissue preparation (author's transl)].

A simple staining technique for gastrointestinal tract neurons in Auerbach's plexus is proposed in thick tissue preparation. The ganglion cells between two muscular layers are easily seen by optical microscope examination in "spread" preparations up to 300 micron in thickness, following dehidratation. Routine paraffin sections can be used. Neurons in thicker preparations (over 300 micron) like in human small intestine can also be seen but only by using small to medium magnifications. A special fixation procedure was developed for this technique.

Adult↗
PubMed1977

Techniques for histological reconstruction of brain stem recording sites.

The masseter muscle of Sprague-Dawley rats was injected with 10 mg of horseradish peroxidase. The electrical activity of the trigeminal motor nucleus was investigated 24-36 hours later using micropipettes filled with a 2.0 M NaCl-7% fast green FCF solution. A parallel series of penetrations at 200 micron intervals was made through the motor complex at known depths. The grid formed from these penetrations was reconstructed using the following techniques. Marks were made at known depths in one or more of the tracts by iontophoresing fast green from the microelectrode tip with a hyperpolarizing current of 10 muA for 10 minutes. To assure proper alignment of the tissue containing the green marks, two agar-India ink plugs were placed caudal and parallel to the recording sites. The frozen tissue was sectioned on a microtome, first through the agar plugs and then through the tissue containing the green marks. The tissue could be incubated for the peroxidase reaction product or stained for Nissl substance. These combined procedures offer a means to correlate the structure and function of brain stem nuclear groups.

Agar↗
PubMed1978

An improved technique using dental histology for estimation of adult age.

Multiple regression analysis has allowed considerable improvement of age estimates on adult human teeth. Not only were the estimates more precise, but they also involved fewer variables, decreasing the probability of observer error. There was consistent evidence that the second molar (Position 7) was the best to use for histological aging techniques. The reduction in variables to just secondary dentin and root transparency has also resulted in a technique that can be used with some confidence in populations other than the one sampled. Thus, dental aging can be used in the same way as epiphyseal fusion, osteon aging, cranial sutures, and changes in the public symphysis that have been used with other contemporary and prehistoric populations.

Adult↗
PubMed1979

A differential staining technique for vertebrate histology.

A staining method is described for studying micro-anatomy of different vertebrate tissues in the light microscope. A staining sequence of celestin blue--erythrosin--orange G--fast green with mordanting in phosphomolybdic acid yields a satisfactory differentiation and fine colour contrast in various tissues. The efficacy of the method was tested on different avian and mammalian tissues.

Adipose Tissue↗
PubMed1976

Methodoloigcal study on examiniation of primary tumors of the respiratory tract "in toto" and histologically.

The results of histological evaluation of DENA-induced tracheobronchial tumors in the Syrian golden hamster are described and compared with those of an in toto method of visualization. In 55 animals, roughly twice as many tumors were detected in the lungs in the in toto preparation as those found by the histological technique [92:42]. The examiniation of the respiratory tract, as a whole, would consequently appear to afford an efficient and economical means of quantifying the effects of, for instance, chemotherapeutic agents in the growth of such tumors, which can subsequently be complemented, especially in the qualitative sense, by histological techniques.

Animals↗
PubMed1977

Treatment failure sites according to irradiation technique and histology in patients with endometrial cancer.

Three hundred and seventy-two patients with endometrial cancer treated from 1948 through 1969 and having complete follow-up were reviewed. All patients completed definitive therapy with preoperative irradiation and surgery or irradiation alone and have separated into two main groups: 1) those treated with irradiation by radium alone or 2) those treated with external irradiation plus diminished amounts of radium. Pathology material from each patient was reviewed by one pathologist. Treatment by radium alone led to fewer pelvic failures than did external irradiation plus diminished amounts of radium, when evaluated by stage, grade, and specific histology. Both irradiation modalities led to about the same incidence of failures due to distant metastasis only.

Adenocarcinoma↗
PubMed1976

En bloc transplantation of liver, pancreas, duodenum, and spleen in the rat. Description of technique and histological findings.

En bloc transplantation involving liver, spleen, pancreas, and duodenum was successfully carried out in the rat. While operative mortality due to technical failure was 31%, of those that survived over 95% of the isografts had normal histology up to 4.5 months. Rejection reaction in the allografts first appeared in the liver and the spleen, followed by the pancreas and the duodenum, and was complete by day 14. No gross evidence of graft-versus-host reaction was observed.

Animals↗
PubMed1979

Methodological aspects of some inner ear vascular techniques.

Modifications of preparation technique assessing cochlear vasculature together with suggestions regarding their applicability to various types of research projects are presented. Detailed information is given regarding the 'soft-surface' dissection and preparation technique along with selected parameters for evaluating the cochlear vasculature. Some basic requirements for any histological technique studying the inner ear blood supply are suggested.

Animals↗