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Results for “Hematoxylin”

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At least 19 recordsLinked to original sources
PubMed1998

Effect of prolonged staining with hematoxylin on detecting Helicobacter pylori in hematoxylin-eosin-stained gastric mucosa.

Helicobacter pylori (H. pylori) is identifiable in hematoxylin-eosin (HE)-stained preparations, particularly under a prolonged hematoxylin staining condition. The staining intensity of H. pylori bodies was increased when the hematoxylin staining period was doubled from 5 min, the routine period of Meyer's hematoxylin staining, to 10 min. The staining intensity of the background epithelial nuclei was only mildly increased. Hematoxylin-eosin staining employing a prolonged hematoxylin staining period gave detectability of H. pylori comparable to May-Giemsa and immunoperoxidase staining. The modified HE method is thus very useful and practical for identifying H. pylori in routine gastric biopsy specimens.

Adult↗
PubMed1991

New investigations on hematoxylin, hematein, and hematein-aluminium complexes. I. Spectroscopic and physico-chemical properties of hematoxylin and hematein.

Analytically pure hematoxylin (Htx), pentaacetylhematoxylin (PAHtx), and hematein (Hm) were isolated and characterized by 1H-NMR spectroscopy. The VIS/UV spectra of Htx and Hm were recorded in MeOH and in H2O at various pH values. The molar extinction coefficients of the long wavelength absorption bands are reported. The pKa value for the 1st acidic dissociation step of Hm has been determined from the pH dependency of the absorption spectra of Hm in aqueous buffer solutions. Finally, the absorption spectra are qualitatively discussed.

Aluminum↗
PubMed1977

Staining of human pituitary glands with lead hematoxylin in comparison with other histochemical procedures, including the immunoenzyme technique.

Human pituitary glands and pituitary adenomas, obtained by surgery or at autopsy, have been stained with lead hematoxylin and the results were compared with those of other histochemical procedures, including the immunoperoxidase technique. Lead hematoxylin positivity was found in the cytoplasm of melanocorticotroph, thyrotroph as well as gonadotroph cells, indicating that lead hematoxylin has no selective affinity for melanocorticotroph cells. Lead hematoxylin positive cells also stained with the PAS technique. Lead hematoxylin positivity was not revealed in growth hormone cells or in prolactin cells. Basophilic adenomas, composed of melanocorticotroph cells and associated with Cushing's disease or with Nelson's syndrome or unaccompanied by hormonal hypersecretion, showed intense lead hematoxylin positivity. Thus, no correlation was evident between lead hematoxylin positivity and secretory activity. Acidophilic or chromophobic adenomas consisting of growth hormone cells, prolactin cells, stem cells, undifferentiated cells or oncocytes, however, were negative. It can be concluded that the lead hematoxylin staining techniques a reliable, easy and reproducible histochemical method, and its use can be recommended in studies related to adenohypophysial cytology.

Adenoma↗
PubMed2002

Specific staining of tissue components with metal-hematoxylin complexes.

The older metal-hematoxylin stains stain a broad spectrum of tissue components. Several recently introduced metal-hematoxylin stains are highly selective. This selectivity is usually bought at the price of severe limitations on the choice of fixative. A very dilute (2 x 10(-4)M) aluminum hematoxylin is selective for nucleic acids in tissues fixed in organic solvents alone. Vanadate hematoxylin is selective for basic proteins in tissues fixed in formaldehyde or mercuric salts. Bismuth hematoxylin is selective for arginine residues and thus for histones and myelin basic protein in tissues fixed in strong acids (Bouin's fluid or SUSA fluid). Zirconyl hematoxylin is selective for acidic mucins. Zirconyl hematoxylin does not restrict the choice of fixative.

Animals↗
PubMed2006

Virtual microscopy in prostate histopathology: simultaneous viewing of biopsies stained sequentially with hematoxylin and eosin, and alpha-methylacyl-coenzyme A racemase/p63 immunohistochemistry.

PURPOSE: Histopathological diagnosis of small focus carcinomas in prostatic needle biopsies is often assisted by IHC. To make a definitive diagnosis the pathologist must compare IHC findings with hematoxylin and eosin stained tissue morphology. We introduce what is to our knowledge a new application of virtual microscopy, in which hematoxylin and eosin, and IHC stains done sequentially on the same microscope slide can be simultaneously displayed and compared on a computer screen. MATERIALS AND METHODS: A total of 30 hematoxylin and eosin stained prostatic needle biopsies were scanned with a computer controlled microscope. The slides were destained and then immunostained with a cocktail of AMACR and p63 antibodies, which labels the nuclei of nonmalignant basal cells (p63) and the cytoplasm of neoplastic glandular cells suspicious for malignancy (AMACR). The slides were then scanned again and the pairs of virtual slides were aligned for synchronized viewing. RESULTS: The presented technique was found helpful when suspicious lesions were small and when examining the immunoprofile of specimens was warranted, in addition to examining hematoxylin and eosin stained tissue morphology. The usefulness of our approach based on virtual microscopy can be evaluated on the website , which also serves as an educational tool for self-learning the correlation between hematoxylin and eosin stained tissue morphology, and AMACR/p63 IHC in prostate biopsies. CONCLUSIONS: The technology for simultaneously viewing sequentially hematoxylin and eosin and IHC stained prostate biopsies can be readily used for educational purposes, as exemplified by our website, and along with the availability of rapid virtual slide scanners it can also be used for clinical diagnostics.

Biopsy, Needle↗
PubMed1978

PAS-lead hematoxylin as a stain for small-granule endocrine cell populations in the lungs, other pharyngeal derivatives and the gut.

Epithelial cells of the several subtypes that comprise the small-granule cell population of the respiratory system are little studied, partly because adequate silver, monoamine fluorescence and other specific light microscopical preparations have been more difficult to obtain than in the gut and other organs possessing diffuse endocrine systems. Periodic acid-Schiff (PAS) in combination with MacConaill-Solcia's lead hematoxylin has in our hands proven dependable for routine staining of serial 2-micrometer glycol methacrylate sections used in mapping the distributions of these cells along the airway. In lungs of mice, hamsters, kittens, and fetal rabbits, typical small-granule cells stain weakly or not at all with lead hematoxylin alone, hence are easily overlooked. PAS adds to the cytoplasm a diffuse magenta coloration; and because it is diastase-resistant, less brilliant than that of mucus but more so than bronchiolar cell secretions, and finer textured than lysosomal staining of other cells present, the effect is to highlight small-granule cells whether solitary or in clusters. Additional PAS staining of basement membranes and lead hematoxylin staining of cilia enhance the combined stain's resolving power. In thyroid gland, parafollicular cells stand out boldly against follicular elements; in small intestine, hematoxylin-positive endocrine cells are well differentiated from absorptive, mucous, and Paneth cells that surround them. Using a complementary monoamine fluorescence technique on plastic sections of lungs from control and 5-hydroxytryptophan-pretreated animals prior to staining, we can show that fluorescent epithelial cells are identical with those stained by PAS-lead hematoxylin.

Adrenal Medulla↗
PubMedDate not supplied

The long history of hematoxylin.

Hematoxylin is a naturally occurring chemical used as the basis of a dye in laboratories throughout the world to stain nuclei in microscope slide preparations. This chemical is extracted from the logwood tree Hematoxylon campechianum and was discovered by Spanish explorers to the Yucatan in 1502. A vigorous trade soon developed related to growing and preparing hematoxylin for use in dyeing fabrics in Europe. In the mid 1800s, amateur microscopists first used hematoxylin to stain cellular components. Later scientists developed a wide range of techniques to demonstrate different cellular components. Hematoxylin remains the most popular nuclear stain in histology. This paper briefly describes the history of hematoxylin production and use in histology.

Cell Nucleus↗
PubMed2003

Staining of intestinal protozoa with Heidenhain's iron hematoxylin.

Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated.

Animals↗
PubMed1984

Ultrastructure of renal glomerular hematoxylin bodies.

Hematoxylin bodies are considered to be the only pathognomonic feature of systemic lupus erythematosus. In this paper, the ultrastructure of hematoxylin bodies is described in a patient with active lupus glomerulonephritis. For identification of hematoxylin bodies, electron micrographs at low magnification were compared with hematoxylin-eosin-stained, Epon-embedded sections from the same block. One electron micrograph at low magnification displayed a whole glomerulus. The bodies were then studied under higher magnification. Hematoxylin bodies were usually in and around the mesangial cells or in the monocytes in mesangial areas. The bodies appeared to contain elements of cellular origin, i.e., aggregates of clumped chromatin, degenerated granules, vesicles, and vacuoles. Sometimes they also contained electron-dense substances of the same density as electron-dense deposits in the mesangial and subendothelial areas.

Adult↗
PubMed1977

Ultrastructural appearance and morphogenesis of renal glomerular hematoxylin bodies.

The fine morphology of hematoxylin bodies--structures which, by light microscopy, are considered to be pathognomonic for systemic lupus erythematosus--is described in renal glomeruli. These bodies are highly pleomorphic and are predominantly of cellular origin. The nuclear components, which are responsible for the characteristic tinctorial properties, appear as aggregates of clumped chromatin and/or irregular masses of finely granular, moderately dense, lobulated material, generally enveloped by membranes. The cytoplasmic components consist of a multitude of vesicles, vacuoles, degenerating granules, and glycogen. Hematoxylin bodies are usually contained within the bounderies of mesangial cells. A transition from circulating leukocytes to typical hematoxylin bodies could be demonstrated. It is suggested that the changes in leukocytes which culminate in hematoxylin bodies are intiated by the action of antinuclear antibodies and also possibly by the ingestion of immune complexes. This proposed appearance and morphogenesis of hematoxylin bodies is in general agreement with a few previously published investigations on these structures and also with the in vitro counterpart, the lupus erythematosus (LE) cell.

Antibodies, Antinuclear↗
PubMed1991

Indium (III)-hematoxylin as a staining and contrasting agent for light and electron microscopy.

A deeply violet indium (III)-hematoxylin complex is formed when indium trichloride is added to an aqueous solution of oxidized hematoxylin. Treatment of glutaraldehyde fixed and Araldite embedded sections of rat seminiferous tubules with indium-hematoxylin revealed a definite staining and contrasting pattern. Semithin sections showed chromatin and nucleoli in violet-blue. Under the electron microscope, chromatin, nucleoli, ribosomes, synaptonemal complexes, chromatoid bodies, membranous components, and microtubules from sperm tails presented high electron opacity, while the acrosome and basement membrane appeared with a lower contrast. This performed indium-hematoxylin complex, which shows an absorption peak at lambda = 560 nm with shoulders at about lambda = 440 and 400 nm, could be valuable as a new staining and electron contrasting agent.

Animals↗
PubMed2003

Zirconyl hematoxylin vs. alcian blue staining in a case of extramammary Paget's disease.

BACKGROUND: Despite its occasional unavailability, alcian blue has long been the stain of choice for the pathognomonic mucin-secreting cells of extramammary Paget's disease. Zirconyl hematoxylin is a recently developed substitute for alcian blue, which is made from common laboratory materials. METHODS: We have stained sections from three different areas of the same case of extramammary Paget's disease with zirconyl hematoxylin, destained in 7% HCl in ethanol, restained with alcian blue at pH 2.5, destained in 2.5% trifluoroacetic acid in dichloromethane, and restained with zirconyl hematoxylin. RESULTS: Each staining procedure stained the same cells. CONCLUSIONS: Zirconyl hematoxylin is a useful alternative to alcian blue in the diagnosis of extramammary Paget's disease.

Aged↗
PubMedDate not supplied

Use of an improved zirconyl hematoxylin stain in the diagnosis of Barrett's esophagus.

Barrett's esophagus is a precancerous condition characterized by replacement of the normal stratified squamous epithelium by a simple columnar epithelium with goblet cells that secrete an acidic mucin. As originally formulated, fresh solutions of zirconyl hematoxylin stain goblet cells poorly. An improved formula, quintupling the amount of oxidant, yields zirconyl hematoxylin solutions that stain goblet cells darkly even when fresh. The improved zirconyl hematoxylin can be used in place of alcian blue in the diagnosis of Barrett's esophagus. The ingredients of zirconyl hematoxylin are always readily available and are generally recognized as safe.

Alcian Blue↗