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PubMedDate not supplied

Historical review and assessment of clinical hemoglobinometry in the United States.

An historical review of clinical hemoglobinometry has been presented. Investigators who have made important contributions to our knowledge of hemoglobin have been cited. A questionnaire was distributed to 750 clinical laboratories in the United States to ascertain (a) the type of instrumentation used routinely to measure hemoglobin; (b) the methods that are now being used for clinical hemoglobinometry; (c) reference material that is used for daily quality control; and (d) the methods used for primary standardization. The results of the 263 responses to the questionnaire are reported. It is noteworthy that at the present time, 78 percent of the responding laboratories use the same instrumentation system. An assessment has been made of the precision of hemoglobin measurements undertaken in clinical laboratories in the United States over the past four decades. The data indicate that hemoglobin measurements during the past four years have become significantly more precise. There is probably no single factor responsible for this timely improvement; however, it may be inferred that the shift from manual to automated methodology in recent years is an important contributing factor.

Europe↗

PubMed1983

Nondiscrete heterogeneity of human erythrocytes: comparison of Coulter-principle flow cytometry and Soret-hemoglobinometry image analysis.

In the blood of normal subjects, the volumes of single erythrocytes are distributed with a coefficient of variation (CV) of 10.8 +/- 1.8%; while in hemoglobinopathies, CV increases proportionately to the degree of anemia produced. Using single cell Soret-band hemoglobinometry and focused-aperture impedance counting, we compared the distribution of red cell volume, area, hemoglobin content, and hemoglobin concentration in normals and subjects with anemic disorders. The CV, nondiscrete heterogeneity, is first, a general characteristic of biologic measurement, second, a sensitive indicator of abnormality of erythropoiesis, and third, consistently less for hemoglobin concentration than for volume, area, or hemoglobin content of the same cells.

Analysis of Variance↗

PubMedDate not supplied

Bedside hemoglobinometry in hemodialysis patients: lessons from point-of-care testing.

The HemoCue B-hemoglobin test system (HemoCue, Inc., Mission Viejo, CA) is a photometric method for rapid bedside determination of hemoglobin (Hb). We compared the performance of HemoCue measured Hb against Coulter STK-S (CSTK) measured Hb in chronic hemodialysis (HD) patients in two different settings. In the first setting, Hemocue analysis was performed by multiple HD technicians (n = 132). In the second setting, a nurse trained in proper specimen handling performed the HemoCue analysis (n = 74). Simultaneous measurement of Hb by the CSTK method was performed. First setting: Hb was 11.1+/-1.66 (SD) g/dl by CSTK and 11.7+/-2.29 g/dl by HemoCue. The HemoCue method consistently overestimated Hb by an average (SD) of 0.63 (1.267) g/dl (95% CI = 0.42 to 0.85). Hb was overestimated in 25.7% and underestimated in 2.3% of the patients by 1 g/dl or more. Thus, the HemoCue system was accurate within 1 g/dl only 72% of the time. Second setting: HemoCue overestimated Hb by an average (SD) of 0.29 (0.52) g/dl (95% CI, 0.17 to 0.41). Only 4% of all patients had errors in estimation of 1 g/dl or more. Thus, HemoCue was accurate in 96% of the patients within 1 g/dl. After reviewing the two protocols, the primary difference in the two studies was the technique used to obtain the specimens. When performed properly, Hb testing using the HemoCue testing system had a high level of agreement with CSTK. Appropriate training in specimen handling, as well as test performance, will increase accuracy and reliability of bedside hemoglobinometry.

Adult↗

PubMed1988

Hemoglobinometry: evaluation of a new method with a stable primary standard.

Using purified chlorohemin as a stable standard, a newly described hemoglobinometry method was evaluated. All blood samples as well as chlorohemin gave identical absorption spectra in an alkaline-detergent solution with a broad peak at 575 nm. Both the reagent mixture and the chlorohemin standard were stable for at least 250 days when stored between 8 degrees C and 32 degrees C in brown glass bottles. The new method showed close correlation when compared to the cyanmethemoglobin technique. Compared to the cyanmethemoglobin method the advantages included a stable standard, shorter conversion time, reduced plasma background and improved hemoglobin ligands conversion. We conclude that the new method confers significant advantages over the cyanmethemoglobin method and in particular provides a stable primary standard with a long shelf-life.

Hemin↗

PubMed1997

[How to achieve analytical reliability of hemoglobinometry].

Three kits of reagents manufactured by RENAM Research and Production Unit provide the analytical reliability of hemoglobinometry. The kits contain: 1) dry powder transforming agent; 2) four calibration standard solutions of hemoglobin cyanate requiring no dilution; 3) three highly purified stable solutions of hemoglobin (simulating human blood) with attested concentrations of hemoglobin from 50 to 200 g/liter.

Hemoglobinometry↗

PubMed1965