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Results for “Gelatinases”

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At least 19 recordsLinked to original sources
PubMed1977

Isolation of Bacteroides corrodens and Eikenella corrodens from human clinical specimens. Comparative study of incidence and methods of identification.

In the course of a systematic search for Bacteroides corrodents and Eikenella corrodens in clinical specimens submitted for microbiological analysis, 61% of the specimens from anal abscesses, 6% of the vaginal specimens and none of the pharyngeal specimens yielded B. corrodens, whereas E. corrodens was recovered from only 9% of the pharyngeal specimens. Some characteristics were found to be useful in differentiating between the two species: B. corrodens strains were strictly anaerobic, cytochrome-oxidase-negative, urease-positive and gelatinase-positive; they were sensitive to lincomycin but resistant to vancomycin. E. corrodens strains on the other hand were facultatively anaerobic, oxidase-positive, urease-negative and gelatinase-negative; they were resistant to lincomycin but sensitive to vancomycin. The pathogenicity of the two species was difficult to assess as in most cases they were recovered from mixed cultures.

Anti-Bacterial Agents↗
PubMed1979

A latent gelatin specific proteinase of human leucocytes and its activation.

1. Gelatin specific proteinase (gelatinase) exists in human leucocytes extracts mainly in a latent form. 2. It is activated by different proteinases as well as by some chemicals (urea, NaSCN, HgCl2). 3. Non-proteolytic activation of latent gelatinase and the decreasing of its molecular weight associated with it strongly suggests that it is an enzyme-inhibitor complex.

Endopeptidases↗
PubMed1977

Preenrichment procedure for detection of Salmonella in gelatin.

Trypticase soy broth was superior to nutrient and lactose broths as a preenrichment medium for the detection of Salmonella in artificially and naturally contaiminated gelatin. The detection rate for Salmonella were further enhanced when homogenization of the gelatin-broth mixture was accomplished by the use of gelatinase rather than by heating of 45 degrees C. Detection rats were also increased by adjusting the pH of the gelatin-broth mixture of 7.0, optimum pH for gelatinase (EC 3.4.23.2) activity.

Culture Media↗
PubMed1975

Extracellular proteins in five clostridial species from human infections.

347 strains from human infections were identified by gas-liquid chromatography of metabolic products and by conventional tests. Simple agar-plate assays were used to analyze the ability to form extracellular proteins. More than 90% of all strains were hemolytic on agar containing rabbit erythrocytes and all were gelatinase producers. All strains of C. bifermantans, C. sordelli, and C. sporogenes were also caseinolytic on skimmed-milk agar, but strains of C. perfringens, C. novyi types A and B were not. Less than 10% of C. perfringens strains were producers of elastase and staphylolytic enzyme and all other species were non-producers. All C. perfringens, C. novyii, C. bifermentans, and C. sordelli were lecithinase producers, but C. sporogenes was not. All strains of C. sporogenes formed deoxyribonuclease, while a varying number of the other species showed a positive reaction.

Bacterial Proteins↗
PubMed1976

Secretion of macrophage neutral proteinase is enhanced by colchicine.

We have studied the effect of colchicine and related compounds on secretion of enzymes by thioglycollated-elicited mouse peritoneal macrophages in culture. Colchicine stimulated secretion of inducible neutral proteinase activities of elastase (EC 3.4.21.11), collagenase (EC 3.4.24.3), gelatinase (pepsin B; EC 3.4.23.2), and azocaseinase 2- to 6-fold for a period of several days, but inhibited the production and release of lysozyme (mucopeptide N-acetylmuramoylhydrolase; EC 3.2.1.17), a noninducible macrophage secretory product. Parallel changes were observed in cell morphology and secretion after treatment with colchicine, Colcemid, and vinblastine, but not with lumicolchicine, and these effects could be gradually reversed by withdrawal of colchicine. Cytochalasin B also stimulated secretion of elastase 2- to 3-fold but did not influence release of lysozyme. These results demonstrate that tubulin-binding drugs may have opposite effects in macrophages than those usually reported for other experimental systems and also provide evidence for the nonparallel discharge of different macrophage secretion products.

Colchicine↗
PubMed1975

Identification of Actinomyces viscosus from canine infections.

Actinomyces viscosus is a gram-positive, non-acid-fact, facultative, catalase-positive, filamentous, or diphtheroidal microorganism. It was isolated from six canine infections during a period of 1.5 years. The organism was cultured from exudate and flaky granules aspirated from infectious granulomas and empyemas. All cultures grew well aerobically and anaerobically with the addition of 10% carbon dioxide. They fermented lactose, produced catalase and acetylmethylcarbinol, reduced nitrates, hydrolyzed aesculin, and did not produce gelatinase or urease. These physiological characteristics distinguish A. viscosus from other morphologically similar organisms.

Actinomyces↗
PubMed1975

Virulence factors of biotypes of Staphylococcus epidermidis from clinical sources.

The biotyping scheme of Baird-Parker was applied to cultures of Staphylococcus epidermidis from patients. In all, 63.6% of 228 cultures belonged to biotype 1, followed by biotypes 4, 3, and 2 in decreasing order of incidence. When classified according to clinical source of isolation, cultures of S. epidermidis were most frequently isolated from urine, with 39.5% of 228 cultures from this source. Each of the four biotypes was distributed throughout all nine catagories of clinical sources. The production of virulence factors was based on the results of three groups of tests: (i) deoxyribonuclease, urease, gelatinase, caseinase, and lysozyme production; (ii) lipolytic activity on the tweens; and (iii) hemolysin production. Enzymatic activity was highest for organisms in biotypes 1, followed by biotypes 3, 4, and 2 in decreasing order. Of the 228 cultures, 76.3% were lysed by lysostaphin. Resistance to antibiotics was highest for tetracycline, ampicillin, and penicillin, with rates of 54.8, 69.3, and 81.6%, respectively. The role of S. epidermidis as an etiological agent was studied by analyzing the laboratory and clinical data of 80 patients selected at random with bacteriuric S. epidermidis. Organisms in biotype 1 were most commonly associated with urinary tract infection. The significance of certain biotypes of S. epidermidis as opportunistic pathogens among compromised hosts in a hospital environment is discussed.

Anti-Bacterial Agents↗
PubMed1977

Enzymatic and hemolytic properties of Propionibacterium acnes and related bacteria.

The production of chondroitin sulfatase, hyaluronidase, deoxyribonuclease, gelatinase, phosphatase, lecithinase, and hemolysins was examined in 95 strains of Propionibacterium acnes and four related species of anaerobic, respectively, microaerophilic coryneform bacteria (P. avidum, P. lymphophilum, P. granulosum, and Corynebacterium minutissimum). All enzymes could be demonstrated in at least one representative of the species tested. Those Propionibacterium species most frequently found in acne vulgaris lesions, i.e., P. acnes and P. granulosum, proved to be the most active organisms concerning the production of the enzymes tested. P. avidum, on the other hand, showed the highest rate of hemolytic activity.

Chondroitinsulfatases↗
PubMed1975

Observations on the distribution and ecology of Clostridium botulinum type E in Alaska.

Environmental samples collected along the coastline and from the interior of Alaska were examined for the presence of Clostridium botulinum. Clostridium botulinum type E was detected in soils from 5 of 12 beaches; in 7 of 115 non-coastal soil samples; in sediments from six of eight locales; in gills of salmon from two fishing areas; and in the feces of 1 of 44 colonic samples from marine mammals. The basic biochemical characteristics of the isolates were determined. Tube tests for demonstrating gelatin liquefaction proved insensitive with these strains, whereas a plate test detected gelatinase in all isolates. The presence of multiple nidi and the continual discharge of organic materials into the environment may contribute to the perpetuation of botulinum spores by which foods prepared form marine animals become contaminated. An emphasis should be placed upon the need for measures to reduce environmental contamination, to reduce contamination during food preparation, and to alert continually the population of the hazard wherever botulism is endemic.

Alaska↗
PubMed1975

Characteristics of Staphylococcus aureus associated with lysogenic conversion to loss of beta-hemolysin production.

Staphylococcus aureus strains 7-8 and 57 that produce beta-hemolysin but not staphylokinase (beta + K-) were lysogenically converted by certain serological group F bacteriophages to the loss of beta-hemolysin production and the gain in staphylokinase production (beta-K+). Serological group A phage 42E was found to convert S. aureus strains 7-8(beta-K-) and 57 (beta + K-) to beta - K-. Conversion of beta-hemolysin by lysogenization of a serological group A phage has not previously been reported. Phage 42E conversions differed from the group F conversions since staphylokinase was not affected. This indicates that conversion to beta-K+ involves separate loci on the phage chromosome. Several characteristics associated with virulence of staphylococci of human or animal origin other than staju;plomase production (coagulase, DNase, lipase, gelatinase, mannitol fermentation, and phage-sensitivity patterns) were not correlated with lysogenic conversions to loss of beta-hemolysin.

Bacteriophage Typing↗
PubMed1979

Microbiology of Legionnaires' disease bacterium.

Legionnaires' disease bacterium in tissue does not readily react with the Gram stain but can be seen by other stains and direct immunofluorescence. It is a slow-growing, aerobic, gram-negative rod that can be cultivated over a narrow temperature range on Mueller-Hinton agar supplemented either with complex biological mixtures or certain ferric salts and cysteine. The bacterium produces unique, branched-chain fatty acids, catalase, oxidase (weakly), and gelatinase and uses starch while ignoring other carbohydrates. Pigment production is related to tyrosine in the medium. In-vitro studies suggest susceptibility to all antibiotics except vancomycin, but a class 1 beta-lactamase has been demonstrated. Analysis of DNA confirmed the unrelatedness of this bacterium to previously recognized prokaryotes. Diagnosis of the disease has depended largely on serologic test findings and the demonstration of the bacterium in tissue and, occasionally, on isolation. Additional, simpler, and more rapid diagnostic tests should soon be available.

Bacteria↗
PubMed1975

Identification of nonfermentative gram-negative bacteria in the clinical laboratory.

A simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. These organisms included apyocyanogenic Pseudomonas aeruginosa, P. fluorescens, P. putida, P. stutzeri, P. maltophilia, P. putrefaciens, P. cepacia, P. alcaligenes, FLAVOBACTERIUM SPECIES, Bordetella bronchiseptica, Acinetobacter anitratum (Herellea vaginicola), A. Iwoffi (Mima polymorpha), Moraxella species, Alcaligenes odorans and Alcaligenes species. The tests used for identification included production of cytochrome oxidase, amylase, deoxyribonuclease, gelatinase, urease and Beta-galactosidase; motility; oxidation of one per cent glucose and ten per cent lactose; fluorescence; indole, hydrogen sulfide and nitrogen gas production; denitrification of nitrites; growth at 42C; penicillin sensitivity and production of an aromatic odor and greenish discoloration on blood agar. Using this scheme, 85 per cent of 243 isolates (unknowns and reference strains) were identified to genus and species. Of the 15 per cent remaining, 11 per cent were identified as alkaline organisms and four per cent were unidentifiable.

Acinetobacter↗