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At least 19 recordsLinked to original sources
PubMed1986

Prevalence of enteric parasites in homosexual patients attending an outpatient clinic.

A total of 372 pooled stool specimens from 274 homosexual men with diarrhea were submitted for parasitologic examination over a 2.5-year period. Each two-vial pooled specimen set contained portions of stool from 3 consecutive days in Formalin and polyvinyl alcohol. Of the 274 patients, 133 (48.5%) harbored one or more intestinal protozoa, with 161 (43.3%) of the 372 specimens submitted being positive for one or more organisms. The parasites identified included Entamoeba histolytica (71 patients), Giardia lamblia (22 patients), Endolimax nana (106 patients), Entamoeba coli (39 patients), Entamoeba hartmanni (25 patients), Dientamoeba fragilis (3 patients), Iodamoeba bütschlii (2 patients), and Chilomastix mesnili (2 patients). Cryptosporidium sp. (2 patients) and Isospora belli (1 patient) were also detected. Results of this study support the experience of other workers regarding high rates of infection with intestinal parasites in the homosexual population and also indicate that symptomatic individuals belonging to this acquired immunodeficiency syndrome risk group be screened for both common and uncommon intestinal pathogens.

Adult↗
PubMed1989

Coincidence of deficient delayed hypersensitivity and intestinal protozoa in homosexual men.

Stool samples of 134 subjectively and apparently healthy male homosexual volunteers were studied, and in 86 men (64.2%) 190 parasite infestations, up to 6 per host, were identified. The most frequent species were Endolimax nana (40), Entamoeba coli (38), Entamoeba histolytica (34), Entamoeba hartmanni (32), and Iodamoeba bütschlii (22); Cryptosporidium was not found. All 11 isolates of Entamoeba histolytica analysed belonged to a noninvasive zymodeme: 10 were of zymodeme I and I of zymodeme III. In 172 healthy control adults 6 parasite infestations were found. In the homosexual men, a negative tuberculin test was more frequent in the presence than in the absence of parasites. Entamoeba histolytica (p = 0.005) Entamoeba coli (p = 0.013) and Iodamoeba bütschlii (p = 0.004) were associated with the anergy.

Amoeba↗
PubMed1992

Absence of an association between enteric parasites in the manifestations and pathogenesis of HIV enteropathy in gay men. The GI/HIV Study Group.

49 gay men confirmed to be infected with the human immunodeficiency virus (HIV) and 9 HIV seronegative gay men participated in a pilot study comparing clinical status and enteric parasite load with gastrointestinal structure, function and symptomatology. Cases included 16/49 (33%) men who were CDC stage II, 7/49 (14%) who were CDC stage III, and 26/49 (53%) who were CDC stage IV. The mean CD4-lymphocyte count was 476 +/- 199 (SD)/microliter. The prevalence of enteric parasitic flora was similar in HIV seropositive patients and controls. Seven cases had enteric infection with pathogenic agents including 3 patients with Entamoeba histolytica, and 4 patients with Giardia lamblia, one of whom also had cryptosporidiosis. Other cases were most frequently colonized with Blastocystis hominis (44%) and Endolimax nana (41%) regardless of the HIV clinical status. HIV seropositive patients with enteric parasitic colonization tended to have lower mean levels of serum IgA than cases without parasites. Duodenal morphometric mucosal changes demonstrated a significant decrease in the mean villous height (p < 0.01) with no elongation of the crypt depth in HIV-infected patients with and without diarrhea compared to controls. Despite gastrointestinal symptoms including diarrhea and weight loss being more prevalent in HIV infected individuals than controls, no correlations were found between the presence of particular enteric parasites, gastrointestinal symptomatology, the clinical HIV status of the CD4-lymphocyte count, the malabsorption of D-xylose or morphometric changes in the duodenum.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1993

Application of the polymerase chain reaction to the epidemiology of pathogenic and nonpathogenic Entamoeba histolytica.

We used the polymerase chain reaction (PCR) to study the epidemiology of pathogenic and nonpathogenic Entamoeba histolytica in a rural community in Mexico. Formalin-fixed stool samples were used for extraction of DNA. The PCR amplifications were performed using two sets of primers that discriminate between pathogenic or non-pathogenic E. histolytica. A total of 201 randomly selected individuals were studied. Among them, 25 (12%) were diagnosed to be infected with E. histolytica by microscopy; PCR identified 24 of these as positive (sensitivity = 0.96) and of 176 negative individuals, only three were identified as positive (specificity = 0.98). The PCR analysis defined three populations: 14 cases were positive for both pathogenic and nonpathogenic E. histolytica, nine cases were positive for pathogenic and negative for nonpathogenic E. histolytica, and only one case was negative for pathogenic and positive for nonpathogenic E. histolytica. Infection by E. histolytica was strongly associated to infection with Entamoeba coli (odds ratio [OR] = 9.41, 95% confidence interval [CI] = 3.09, 28.65, P < 0.0004) and Endolimax nana (OR = 6.15, 95% CI = 2.03, 18.17, P < 0.0004). This new technique has high specificity and sensitivity; it is simple, reproducible, fast, avoids the need to culture trophozoites, and can be applied in the field for epidemiologic studies.

Adolescent↗
PubMed1975

Surveillance of some infectious diseases among aircrew personnel in Southeast Asia.

A 2-year analysis of specimens among aircrew personnel in Southeast Asia is reported. Stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (SGOT) and antibodies to Entamoeba histolytica, Toxoplasma gondii, Chikungunya and Japanese encephalitis viruses, Rickettsia tsutsugamushi, Leptospira sp. and Pseudomonas pseudomallei. One to four specimens each were obtained from 537 adult males and 20 females. There were 56 subjects with intestinal parasites as follows: Giardia lamblia (3.8%), Endolimax nana (2.6%), Entamoeba histolytica (1.4%), Entamoeba hartmanni (0.9%), Entamoeba coli (0.7%), Trichiuris trichiura (1.7%), Ascaris lumbricoides (1.4%), hookworm (0.9%), and Clonorchis sinensis (1.2%). Two individuals had malaria, Plasmodium vivax (0.6%). Pathogenic enteric bacteria were isolated from seven stool specimens; Shigella groups B and D (0.9%), Salmonella paratyphi (0.3%), and Arizona group (0.9%). Significantly elevated SGOT levels were found in 0.9% and antibodies to Japanese encephalitis virus in 1.5%, to Rickettsia tsutsugamushi in 1.2%, to Pseudomonas pseudomallei in 0.3%, to Entamoeba histolytica in 0.9% and to Toxoplasma gondii in 10.1%. No antibodies were found to Chikungunya virus or Leptospira sp.

Aerospace Medicine↗
PubMed1978

Contribution to the problem of the so-called nonpathogenic amoebae in the intestine of man.

Among 10,418 patients of a Prague hospital, a plain infection with intestinal parasitic protozoans was identified in 1,319 persons (12.7%). Of these, 3.5% were infested with Giardia intestinalis, 0.3% with Entamoeba histolytica forma minuta, 5.7% with Endolimax nana. We evaluated the frequency of findings of protozoans in various clinical diagnoses. A statistically significant increase in frequency was recorded for E. nana in diagnoses of eosinophilia, giardiasis, amoebiasis and helminthiasis. A slight increase above the average was found for Entamoeba coli in diagnoses of giardiasis and helminthiasis. Most cases of infection with Entamoeba histolytica were associated with a stay abroad. No increase in the frequency of these protozoans was recorded for patients with diarrhea. An analysis of the results indicated that a nonpathogenic amoeba might participate in the origin of intestinal disorders in man.

Adult↗
PubMed1984

[A copro-parasitological study on the wrestlers of the national team].

This copro-parasitological research was made on the 18 wrestlers of the national team in the Talas training camp in Kayseri, The majority of them came to our laboratory with gastro-intestinal complaint such as nausea colicky pains anorexia and dyspepsia and other complaints such as exhaustion and fatigue and 16 of them were found infested with one or more than one parasites. As a result of our coprologic examination finding parasites and their infection rate were: Entamoeba histolytica (50%), Entamoeba coli (16,6%), iodamoeba bütschlii (16,6%), Endolimax nana (11,1%), Giardia lamblia (11,1%), Enteromonas hominis (11,1%) Ascaris lumbricoides (5,5%) and Hymenolepis nana (5,5%). It has been indicated that, 11 of total wrestlers were found infected with one kind, 4 of them were found infected with two kinds and 1 was found infested with three kinds parasites.

Amoeba↗
PubMed1983

Parasitic rheumatism presenting as rheumatoid arthritis.

A symmetrical polyarthritis with low titer positive rheumatoid factor occurred in a young man who also complained of chronic diarrhea after returning from Vietnam. Endolimax nana grew on stool culture. Both the patient's diarrhea and arthritis responded effectively to therapy with metronidazole. The diagnosis of parasitic rheumatism was made in retrospect. Diagnostic consideration should be given to this entity in patients with unusual arthropathies, particularly if they have stayed in an endemic parasitic country and have gastrointestinal symptoms.

Adult↗
PubMedDate not supplied

[A coprological study of intestinal infections in Northern Morocco (provinces of Tangier, Tetuán and Larache)].

BACKGROUND: An epidemiological study on intestinal parasites has been carried out in Northern Morocco (province of Tangier, Tetuan and Larache). A comparative Study has been made at a provincial, city and areas (urban and rural) level. Also the SPI (simple parasitism index) and the CPI (comparative parasitism index) distribution. METHODS: The coprological method used were: direct, concentration (Fulleborn, Telemann and Kato) method and ye test technics (Ferric Hematoxylin and E. Chlorazol Black). RESULTS: The number of faecal samples were 4643, parasitism people 2637 and the total number of parasites were 4816. SPI 56.79% and CPI 1.83. Among the Protozoa, Entamoeba coli, followed by Endolimax nana, Giardia lamblia and Iodamoeba buetschlii prevailed, the other species coming a lung distance behind. Among the Helminths, Trichuris trichiura prevailed much more than the others; Ascaris lumbricoides followed and on the third place were Enterobius vermicularis and Hymenolepis nana. The percentage found for E. vermicularis was very high, since an appropriate technique of diagnosis was not used. The data have been statistically tested. CONCLUSIONS: We found a high rate of parasitism in the 3 provinces that have studied and an approximate number of two parasites per person. SPI and CPI are greater in Tetuan and Larache than in Tangier.

Adult↗
PubMed1976

Parasitic infections in humans in West Kalimantan (Borneo), Indonesia.

A survey was carried out among inhabitants of eight villages in West Kalimantan Province (Borneo), whereby blood smears were examined for malaria, stools examined for intestinal parasites and sera tested by the indirect hemagglutination test for antibodies to Entamoeba histolytica and toxoplasma gondii. The prevalence of malaria among 3017 people examined was 5.6% (Plasmodium vivax 2.8%, Plasmodium falciparum 2.8%). Brugia malayi microfilariae were found in 3.6% and Wuchereria bancrofti in 0.3%. Ninety-seven percent of 2101 stool specimens examined contained evidence of intestinal parasites. Trichuris trichiura (90%) was most common followed by Ascaris lumbricoides (76%), hookworm, (60%), Etamoeba coli (23%), Entamoeba histolytica (6%), Endolimax nana (6%), Iodamoeba butschlii (4%), Giardia lamblia (3%), Chilomastix mesnili (1%) and Strongyloides stercoralis (1%). Other parasites found were Entamoeba hartmanni, Trichomonas hominis, Balantidium coli, Enterobius vermicularis, Hymenolepis nana, Echinostoma sp. and Physalopterid, Dicrocoeliid, and Heterophyid type-eggs. The amoeba prevalence rate was 30%. Indirect hemagglutination antibody titers equal to or greater than 1:128 for Entamoeba histolytica and 1:256 for Toxoplasma gondii were detected in 7% and 3%, respectively, of 1511 sera tested.

Adolescent↗
PubMed1981

[Parasitic disease in schoolchildren in a village in Swiss Jura].

In a village of the Swiss Jura we examined 134 schoolchildren (7 to 16 years old) clinically, parasitologically and serologically for parasites. Half of the children showed an insufficient hygiene of hands, feet and/or anus. In 7 out of 107 anus-scotch-tests there were eggs of Enterobius vermicularis. Protozoans could be demonstrated in 8 of 133 MIF-stool-samples (2 cases of Giardia lamblia, 2 Endolimax nana and 4 Jodamoeba bütschlii). Using the procedure of enrichment of Telemann no helminth eggs were detected. Altogether parasites were found in 10% of the children. 31 children have antibodies against Toxoplasma gondii (IF titre greater than or equal to 1:40). 5 children showed a positive Toxocara-serology, additional 5 an uncertain positive reaction. The immunodiagnostic of Echinococcosis (ELISA, IF, IHA, CIE) was doubtfully positive in 4 children. In this study, intestinal protozoans and E. vermicularis were the most commonly occurring parasites followed by asymptomatic toxocariasis.

Adolescent↗
PubMed1979

Electrophoretic isoenzyme patterns of the pathogenic and non-pathogenic intestinal amoebae of man.

Cultured stocks of Entamoeba hartmanni, Endolimax nana, Iodamoeba buetschlli and Dientamoeba fragilis were compared with the four Entamoeba histolytical groups already described (SARGEAUNT et al., 1978), by the electrophoretic patterns of three enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM) and L-malate: NADP+ oxidoreductase (oxalacetate-decarboxylating) (ME). All the species were easily distinguished by their characteristic patterns.

Amoeba↗