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At least 19 recordsLinked to original sources
PubMed1991

Myocardial dysfunction and norepinephrine release in the isolated rat heart injured by electrolysis-induced oxygen free radicals.

In the present investigation, we used electrolysis as a source of oxygen free radicals to test their possible role in norepinephrine release, as well as in the mechanism of cellular injury, cardiac dysfunction and arrhythmias. In the isolated rat heart perfused under constant pressure, according to the Langendorff technique, electrolysis of the Krebs-Henseleit solution (10 mA d.c. current for 1 min) produced myocardial irreversible dysfunction within 5 min. Fifteen minutes after electrolysis, significant falls in the left ventricular pressure (from 87.5 +/- 6.8 to 33.7 +/- 5.2 mmHg), dP/dt max (from 1230 +/- 90 to 375 +/- 59 mmHg/s), heart rate (from 287 +/- 18 to 119 +/- 13.5 beats/min) and coronary flow (from 14.8 +/- 9 to 3.4 +/- 1.7 ml/min) were observed, along with an increase in left ventricular end diastolic pressure from 10 to 50 +/- 3.5 mmHg (n = 8, P less than 0.01). AV conduction block and/or sinus bradycardia were noted in all preparations. An increase in norepinephrine washout from 298.5 +/- 84 at baseline to 610 +/- 110 pg/min/g 5 min after electrolysis was measured (n = 8, P less than 0.05) and a 44.8 +/- 9.2% and 35 +/- 7.5% reduction, respectively in right and left ventricular tissue norepinephrine content was also found at 30 min (n = 5, P less than 0.05). Pretreatment of the hearts 10 min before electrolysis and throughout the experimental period by superoxide dismutase (SOD; 100 U/ml), catalase (150 U/ml), a combination of SOD + catalase or mannitol (50 mM) partially blocked the deleterious effect of free radicals and permitted a functional recovery of 50 to 60%, mannitol being the more potent protective agent. Furthermore, these scavengers also significantly reduced norepinephrine washout.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

Electrolysis with different electrode materials and combined with irradiation for treatment of experimental rat tumors.

In order to make electrolytic tumor destruction more effective new electrode materials were tested (Part I) as well as a combination of electrolysis and megavolt therapy (Part II). All tests were performed in experimental tumors implanted subcutaneously in rats. Altogether in 41 rats in 5 series (Part I) electrodes made of rhodium (Rh), copper (Cu), or brass (Zn-Cu alloy) were investigated but the effect was not found to be better than that of the previously tested platinum (Pt). Oxidation and corrosion made Rh and Cu electrodes less suitable for electrolysis compared to Pt, while brass electrodes became isolated through zinc-salt-formation and performed unsatisfactorily. The radiosensitizing properties of electrolysis were tested in 55 rats with experimental tumors (Part II). One control group had only Co-irradiation, while in 2 other groups Cu- or Pt-electrolysis of the tumors was carried out before irradiation. The combined treatment resulted in a significantly better effect on the tumors, registered as inhibition of tumor growth or disappearance of tumor. As the electrolyzed, necrotic tissue remained in the tumor the effect might not be mediated through diminished target volume. An inflammatory reaction around the electrolytic lesion with increased blood flow and higher oxygenation of the tumor could cause a more positive response to megavolt treatment.

Animals↗
PubMed1991

Protective effects of ceruloplasmin against electrolysis-induced oxygen free radicals in rat heart.

The potentially injurious effects of oxygen-derived free radicals (OFR) on the myocardium can be prevented in part by pretreatment with OFR scavengers or antioxidants. Since ceruloplasmin (CP) has been shown to possess potent antioxidant activity and scavenge a variety of OFR in vitro, we have undertaken to study its protective effects against myocardial injury induced by OFR. CP was freshly purified by a fast method that minimized proteolytic enzyme degradation. Free radicals were generated by the electrolysis (10 mA DC current for 1 min) of a Krebs-Henseleit solution perfusing an isolated rat heart preparation under constant pressure conditions. CP (0.25 microM) afforded 80 and 63% protection (n = 8, p less than 0.05), respectively, against the deleterious effects of electrolysis-induced OFR on left ventricular pressure and coronary flow. The increase in left ventricular end diastolic pressure used here as an index of heart failure did not occur in the presence of 0.25 microM CP. Moreover, CP significantly reduced the increase of norepinephrine washout in the effluent perfusate after electrolysis suggesting a protection against free radical-induced injury to sympathetic nerve endings.

Animals↗
PubMed1992

Synergism between electrolysis and methylene blue photodynamic action in Escherichia coli.

There is interest in the use of photodynamic therapy for the treatment of certain diseases, including cancer. However, weak penetration of visible light in tissues has restricted its use. In this study the possibility of enhancing photodynamic effects by the use of energies that penetrate more deeply in tissues was investigated. Weak electric currents (1.0 mA) applied to Escherichia coli cells for short periods, producing little or no lethal damage, was found to act synergistically with the photodynamic action of methylene blue, significantly enhancing the effects of this treatment. This synergism exists also between electrolysis and X-rays but not between electrolysis and UV-254 nm. It is suggested that this synergism might eventually be used to improve the results obtained in therapeutic practice based on the utilization of photodynamic action.

Electricity↗
PubMed1992

Assay of thiols and disulfides based on the reversibility of N-ethylmaleimide alkylation of thiols combined with electrolysis.

A simple and specific method for analyzing thiols and disulfides on the basis of the reversibility of N-ethylmaleimide (NEM) alkylation of thiols is described. When the adduct of NEM and glutathione (GSH) was electrolyzed at neutral pH, all of the GSH was recovered. When the adduct was exposed to pH 11.0 for 15 min at 30 degrees C before electrolysis, GSH was not detected. The same behavior was observed after protein thiols reacted with NEM. This pH-dependent production of thiol from the adduct was used to assay GSH and oxidized glutathione in yeast cells, to assay sulfhydryl groups and disulfide bonds in authentic proteins, and to protect thiols from oxidation during enzymatic digestion of protein. This method is useful for assay of thiols and disulfides of both small and large molecules and can be used to identify labile thiols in biological samples that are oxidized during extraction procedures.

Alkylation↗
PubMed1977

Activation analysis of mercury in urine samples of electrolysis cell workers.

The activation method of determination of trace amounts of Hg in human urine has been studied by introducing a simple amalgam deposition method. Human urine samples were obtained from the alkali chloride, electrolysis plant equipped with Hg cells. The capacity of Cu powder for Hg quantity, the pH conditions, and the shaking time were described. The results showed that the Hg contents in workers' urine samples varied from 33 ppb to 60 ppb depending on working conditions.

Activation Analysis↗
PubMed1991

["Layer" distribution of various gaseous chemical substances in the air during zinc electrolysis].

Studies performed at a zinc plant's electrolysis shop revealed a reason accounting for the uneven distribution of different chemical compounds in the working zone air. It was established that the 'layer' distribution of the chemical substances depended on the ir respective specific weights, which is important for hygienic assessments of occupational conditions.

Air Pollutants, Occupational↗
PubMed1991

[Hygienic evaluation of electrolysis oxygen obtained from the water of different chemical composition].

Influence of electrolysis gaseous oxygen (EGO) mixed with nitrogen (1:4) on the living organism was studied in a 30-day 24-hour experiment on guinea pigs. A preliminary conclusion states, that EGO, obtained in the system with a solid polymeric electrolyte out of moist materials of different chemical composition, can be recommended for the formation of artificial gas atmosphere in hermetic objects in the mixture with gaseous nitrogen in the ratio 1:4.

Animals↗
PubMed1978

[Determination of local muscular blood flow in the hindlimbs of dogs following electrolysis of conducting vascular prostheses previously implanted in the abdominal portion of the aorta].

Peripheral blood flow in the posterior limbs in electrolysis of electroconductive vascular prosthesis preliminarily implanted into the abdominal aorta was experimentally determined on 25 mongrel dogs. Following reestablishment of the main blood flow a positive electrical potential (3--4 V) was fed on the prosthesis by means of a current conductor. Tissue blood flow was radiographically studied with xenon-133. Results of this investigation showed that during feeding the positive potential to the electroconductive prosthesis an increase of tissue blood flow occurred in the canine posterior limbs. After the cessation of the current feeding to the prosthesis the tissue blood flow occurred in the canine posterior limbs. After the cessation of the current feeding to the prosthesis the tissue blood flow decreased to the initial values. A conclusion was drawn that a positive potential from the source of direct current should be fed constantly to the electroconductive prosthesis or prolonged stable tissue blood flow increase.

Animals↗
PubMed1978

Formation of amino acids from aliphatic nitriles and aliphatic amines by contact glow discharge electrolysis.

Contact glow discharge electrolyses (CGDE) were carried out relative to the prebiotic formation of amino acids by amination of aliphatic nitrile in aqueous ammoniacal solution, and by cyanization of aliphatic amine by sodium cyanide. The CGDE of propionitrile by amination followed by hydrolysis resulted in the formation of glycine, alanine and beta-alanine. The reaction of ethylamine by cyanization, gave glycine, alanine, beta-alanine, aspartic acid, and serine. In these reactions, a relatively high ratio of glycine was observed. This could be explained by the cleavage of the alpha,beta-carbon bond, which was broken easily, due to the strong electron-attracting property of the nitrile group of propionitrile and the resulting alpha-aminopropionitrile.

Amines↗
PubMed1979

Electrolysis between the feet and the ground and its probable health effects.

This paper describes experiments which show that a person with bare feet on the ground or with conducting sole shoes, sets up between his feet a redox cell, and that current of a significant magnitude flows up one leg and down the other. It suggests that this electrolytic activity could have very wide significance in various aspects of health.

Electrolysis↗