The physical properties of elastic tissue.
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Amino Acids↗
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Enzymatic destruction of the elastic lamella at the mouth of cerebral berry aneurysm? An ultrastructural study with special regard to the elastic tissue.
Destruction of the elastic tissue is probably an acquired lesion and is decisive for the appearance of cerebral arterial aneurysms at the sites of congenital media defects. The elastic component in the mouths of aneurysms has therefore been studied by electron microscopy, using two new staining methods, i.e. ruthenium-red staining and prolonged osmium-tetroxide treatment. The hypertrophic, duplicated, elastic lamellae showed a disintegration of their luminal portions not earlier described. In close connection with the disintegrated portions, extracellular lysosome-like granules were observed. It is hypothesized that discharged leucocyte grannules containing elastase help to destroy the elastic lamellae.
Adult↗
An electron microscopic technique for the study of elastic tissue in small arteries and arterioles of the kidney.
Elastic tissue content and distribution were studied by electron microscopy of small arteries and arterioles of kidneys obtained from normotensive Wistar and spontaneously hypertensive rats, normal dogs and patients with hypertension and glomerular diseases. Specificity and sensitivity of silver tetraphenyl porphyrin sulfonate stain are discussed. This specific electron microscopy technique should prove useful in analysis of elastic tissue in pathologic states of other organs, especially where elastic tissue normally appears to be sparse or absent.
Animals↗
Effect of thiouracil upon canine arterial elastic tissue.
Unlike some other mammalian species, the dog is relatively resistant to the development of elevated levels of serum cholesterol after prolonged cholesterol feeding. This may be overcome by suppressing thyroid activity with thiouracil. Information regarding possible activity of thiouracil itself upon the arterial tissues is almost nonexistent. The present investigation was undertaken to test whether this drug has any such action, especially upon the arterial elastic tissues. Destructive changes were observed in arterial elastic tissues in dogs given thiouracil for three and six months. The changes consisted of accentuation of the elastic fibrillar components, formation and subsequent coalescence of clefts, and fragmentation and ultimate "dissolution" of the elastic elements. The results suggest that thiouracil may exert a damaging effect upon the arterial elastic fibers; thus, it is possible that one of the mechanisms by which thiouracil and cholesterol administration induces experimental atherosclerosis in the dog is by elastic tissue destruction, possibly promoting the subsequent lipid accumulation in the arterial wall.
Animals↗
Elastic tissue dysplasia of the internal carotid artery.
Elongation with kinking of the internal carotid artery is not an uncommon finding. Cerebral vascular insufficiency has been reported secondary to this abnormality. Although arteriosclerosis is a frequent cause in adults, the etiology in children has never been explained. Three children with significant kinking of the internal carotid artery are reported here. Each underwent surgical correction by excision and reconstruction. Histological review showed the elastic tissue to be disrupted, diminished in amount, and unevenly distributed. Retrospective examination of this elastic tissue dysplasia showed it to be localized, there being normal morphology both proximally and distal to the involved area. There was recurrence of elongation and kinking in one patient due to incomplete excision. Correction was accomplished after re-excision of the involved tissue with a vein graft interposition. From this study, it can be concluded that elongation and kinking of the internal carotid artery in children is secondary to elastic tissue dysplasia. It is imperative that the entire involved area be removed in order to prevent recurrence. The excised specimen should be studied histologically with elastic tissue stains. Such stains cannot be performed as frozen section. Should permanent studies show the margins of resection not to be beyond the dysplastic tissue, careful followup of these children is essential to recognize recurrence.
Carotid Artery Diseases↗
Suppression of aortic elastic tissue autofluorescence for the detection of viral antigen.
Suppression of aortic elastic tissue autofluorescence was achieved by employing a modification of Verhoeff's elastic tissue staining procedure. Consquently, coxsackievirus B antigen present in the aortic media was detected by conventional fluorescent antibody staining.
Animals↗
The histomorphology of the elastic tissue system in the skin of the human hand.
This study compares the effectiveness of the Verhoeff and Puchtler-Sweat resorcin fuschin methods of staining the elastic tissue of palmar and dorsal skin specimens of the human hand. The resorcin stain demonstrates the "oxytalan" fibres of the elastic tissue system in the dermal papillary layer of the palmar skin. Oxytalan fibres are not generally demonstrated by the Verhoeff method and are not demonstrated in the dorsal skin specimens. These studies substantiate the existence of a system of elastic tissue fibres in the skin of the hand. The inter-relationship of the elastic and collagenous fibres appears to complement each of their functions; namely, in anchoring the dermo-epidermal junction, absorbing the stresses of stretch and compressive forces and return the tissues to their original state of tension, and providing protection to the dermal appendages in addition to lending a gradient mobility transmitted through the dermis to the subcutaneous layer to give suppleness and mobility to the skin.
Adult↗
Elastic tissue in the limiting membrane of the human seminiferous tubule.
The structure and distribution of elastic tissue were studied in the limiting membrane of the seminiferous tubule from normal human testis. The elastic and elastic-related fibers (oxytalan and elaunin) were recognized by their tinctorial and ultrastructural characteristics. The connective structures of the limiting membrane, including the fibrotubules and the amorphous material of the elastic system, were studied after tannic acid-glutaraldehyde fixation. Fibrotubules (oxytalan fibers) were observed in almost all intercellular spaces of the limiting membrane, closely related to the contractile cells; the elaunin fibers (patches of amorphous material surrounded by bundles of fibrotubules) were evident in the outermost layers. The function of this system of elastic tissue and myoid cells is discussed, considering the permeability membrane and the role of the myoid cells in the elastogenesis and contractility of the seminiferous tubule.
Elastic Tissue↗
Pulmonary elastic tissue in generalized elastolysis (cutis laxa) and Marfan's syndrome: a light and electron microscopic study.
We have studied elastic tissue changes in the lungs from patients with Marfan's syndrome, generalized elastolysis, patients with emphysema of other cause, and those dying from unrelated causes. Degenerative changes were seen in the elastic tissue fibers in patient's with Marfan's syndrome; they varied from mild to severe. Elastic fibers in the lungs of patients with cutis laxa showed by light and electron microscopy the same morphologic changes previously reported in the skin and vessels. While the alterations in individual elastic fibers in the alveolar septa appeared similar, in cutix laxa there was severe involvement of almost all fibers, whereas in Marfan's syndrome the involvement was not so severe, nor did it affect all fibers. On examination of lung sections from patients with cutis laxa or Marfan's syndrome, both were readily separable from normal and abnormal controls.
Adult↗
The elastic tissue of the adult dilated ureter.
Adult dilated ureters have been examined in order to calculate the amount of the elastic tissue. From the histological observations and by the chemical analyses performed on the extracted elastin a decrease of the elastic tissue, as compared to the normal adult ureters has been noted. In fact, in the adult normal ureters, the elastin accounts for 9% of the dry matter, while in the abnormal the elastin represents 6.5%.
Adult↗
[Normal and pathological elastic tissue under the electron microscope on thin and ultrathin sections (author's transl)].
Three simple methods (adapted from optical techniques) for normal and pathological elastic tissue caracterisation in electron microscopy on thin and ultrathin sections are proposed. Two of these methods (orcein and fuchsin resorcin) seem to have a specificity for arterial and breast cancer elastic tissue. Weigert's method gives the best contrast.
Arteriosclerosis↗
Wrinkles due to idiopathic loss of mid-dermal elastic tissue.
Widespread areas of fine wrinkling of the skin had been noted for 2 years by a 42-year-old healthy white woman. Histological study disclosed a selective absence of elastic tissue strictly limited to the mid-dermis of the involved areas. It was postulated that an antecedent urticarial process may have led to such a specific zonal destruction of elastic tissue. The elastolytic process did not change or extend, and no urticarial lesions were seen during a year of observation.
Adult↗
Studies of elastic tissue formation in the developing bovine ligamentum nuchae.
The foetal ligamentum nuchae showed two distinct stages of development, each characterised by its cell population and fibre products. Fibroblasts of the early phase have extensive ergastoplasm apparently associated with collagen production; those of the late phase have an "attenuated" cytoplasm and coincide with an increase of elastic tissue content. The elastic fibre comprised a core of fine elastin fibrils (100--130 nm diameter) and a surrounding mantle of microfibrillar protein. As development proceeded, the elastin fibrils appeared to coalesce with each other and also with elastin fibrils from adjacent fibres to form composite mature elastic fibre--a process which was accompanied by a loss of microfibrils. A characteristic of elastic fibres from adult ligament, not apparent in the foetal tissue, was that of bifurcation.
Animals↗
The identification of glycoproteins associated with elastic-tissue microfibrils.
Cell cultures derived from foetal bovine ligamentum nuchae accumulate extracellular fibrils morphologically identical with elastic-tissue microfibrils. Two glycoproteins synthesized by the ligament cells are closely related to the matrix microfibrils as assessed by immunological and chemical extraction techniques.
Animals↗
Ultrastructural changes of elastic tissue in hamster lung during elastase-emphysema.
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Animals↗
Iron gallein elastic method---a substitute for Verhoeff's elastic tissue stain.
A method for staining elastic fibers in formalin fixed, paraffin embedded sections is described. After deparaffinizing and dehydration, sections are stained for 30 minutes in a solution prepared by mixing equal parts of 1% gallein dissolved in ethylene glycol and absolute alcohol (1:4), and 1.16% aqueous ferric chloride in 1% hydrochloric acid. The sections are washed in water and then differentiated in 2% ferric chloride for 2 minutes. After washing in water, the sections are counterstained with a variant of Van Gieson's picric acid-acid fuchsin for 1 minute. The results are similar to Verhoeff's elastic stain with elastic fibers staining black. An advantage to this staining procedure is that visually controlled differentiation is not necessary.
Aorta↗
Resolution and repair of elastic tissue calcification in pseudoxanthoma elasticum.
Biopsy specimens of a skin lesion from a patient with pseudoxanthoma elasticum were obtained at the beginning and end of a 1 1/2-year period of clinically documented regression of the lesion. Light microscopic studies of these specimens showed that, during the period of regression, there was a decrease in the number of abnormal calcified elastic fibers in the dermis. Electron microscopic findings were interpreted as indicating breakdown and removal of mineralized elastic fibers at the beginning and formation of new elastic fibers at the end of the regression period. Cytoplasmic processes of active fibroblasts were intimately associated with fibers undergoing dissolution. The case thus represents a clinically and morphologically documented example of resolution and repair of elastic tissue calcification.
Biopsy↗
An evaluation of elastic tissue staining.
The Special Procedures Laboratory of the Department of Pathology at M.D. Anderson Hospital was concerned with the nationwide shortage of hematoxylin. The amount of this dye required for various staining solutions was calculated to determine thrifty usage. Some solutions required more hematoxylin than was available and prompted the serious investigation of existing techniques and new methods for routine laboratory use. Evaluation of various techniques resulted in a significantly successful modification of Gomori's aldehyde fuchsin technique, which was subsequently adopted as a routine procedure for elastic tissue staining in the Special Procedures Laboratory.
Aldehydes↗